北京华越洋生物
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首页    载体及质粒    pBTB-5
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pBTB-5

@pBTB-5

 

pBTB-5



 

编号

载体名称

北京华越洋VECT75146

pBTB-5

 

pBTB-5载体基本信息:

载体名称:

pBTB-5

质粒类型:

广宿主载体;克隆载体;原核表达载体

高拷贝/低拷贝:

--

克隆方法:

限制性内切酶,多克隆位点

启动子:

araBAD

载体大小:

3161 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

甲氧氨苄嘧啶

克隆菌株:

DH5α

宿主细胞(系):

多个菌属

备注:

--

组成型/诱导型:

诱导型

 

pBTB-5载体质粒图谱和多克隆位点信息:

 

 

pBTB-5载体序列:

ORIGIN      

        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga

       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg

      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca

      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc

      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag

      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc

      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc

      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg

      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt

      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg

      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg

      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac

      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg

      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa

      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat

      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg

      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca

     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg

     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt

     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg

     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca

     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta

     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat

     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg

     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga

     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca

     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa

     1621 ggaagctaaa atgggtcaaa gtagcgatga agccaacgct cccgttgcag ggcagtttgc

     1681 gcttcccctg agtgccacct ttggcttagg ggatcgcgta cgcaagaaat ctggtgccgc

     1741 ttggcagggt caagtcgtcg gttggtattg cacaaaactc actcctgaag gctatgcggt

     1801 cgagtccgaa tcccacccag gctcagtgca aatttatcct gtggctgcac ttgaacgtgt

     1861 ggcctaagaa tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg

     1921 ctatggaggt caggtatgat ttttatgaca acttgacggc tacatcattc actttttctt

     1981 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga

     2041 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg

     2101 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa

     2161 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg

     2221 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct

     2281 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca

     2341 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc

     2401 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc

     2461 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc

     2521 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt

     2581 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc

     2641 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc

     2701 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg

     2761 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca

     2821 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt

     2881 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta

     2941 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct

     3001 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca

     3061 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta

     3121 ctgtttctcc atacccgttt ttttgggcta gagaattcgt c

//

 

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