北京华越洋生物
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首页    载体及质粒    pLVTH慢病毒载体
1

pLVTH慢病毒载体

pLVTH

pLVTH


 

编号

载体名称

北京华越洋VECT231325

pLVTH

 

pLVTH载体基本信息:

载体名称:

pLVTH

质粒类型:

哺乳动物细胞慢病毒表达载体

高拷贝/低拷贝:

高拷贝

启动子:

EF-1   alpha

克隆方法:

多克隆位点,限制性内切酶

载体大小:

11086   bp

5' 测序引物及序列:

EF-1a   Forward:   TCAAGCCTCAGACAGTGGTTC

3' 测序引物及序列:

EGFP-N: CGTCGCCGTCCAGCTCGACCAG

载体标签:

C-EGFP

载体抗性:

氨苄

备注:

载体使用Stbl3感受态细胞扩增质粒,可以减少载体基因重组几率。该载体已经被pLVTHM载体替代,pLVTHM载体允许shRNA的定向克隆。

稳定性:

/

组成型:

--

病毒/非病毒:

慢病毒

 

 

 

pLVTH载体质粒图谱和多克隆位点信息

 

pLVTH载体序列:

ORIGIN

    1 TTGGAAGGGC TAATTCACTC CCAAAGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC

   61 ACACAAGGCT ACTTCCCTGA TTAGCAGAAC TACACACCAG GGCCAGGGGT CAGATATCCA

  121 CTGACCTTTG GATGGTGCTA CAAGCTAGTA CCAGTTGAGC CAGATAAGGT AGAAGAGGCC

  181 AATAAAGGAG AGAACACCAG CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG

  241 GAGAGAGAAG TGTTAGAGTG GAGGTTTGAC AGCCGCCTAG CATTTCATCA CGTGGCCCGA

  301 GAGCTGCATC CGGAGTACTT CAAGAACTGC TGATATCGAG CTTGCTACAA GGGACTTTCC

  361 GCTGGGGACT TTCCAGGGAG GCGTGGCCTG GGCGGGACTG GGGAGTGGCG AGCCCTCAGA

  421 TCCTGCATAT AAGCAGCTGC TTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG

  481 AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC

  541 TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT

  601 CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA

  661 GCGAAAGGGA AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG

  721 GCAAGAGGCG AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG

  781 AAGGAGAGAG ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG

  841 GAAAAAATTC GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG

  901 GCAAGCAGGG AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC

  961 TGTAGACAAA TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA

 1021 TCATTATATA ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC

 1081 ACCAAGGAAG CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAC CACCGCACAG

 1141 CAAGCGGCCG CTGATCTTCA GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA

 1201 ATTATATAAA TATAAAGTAG TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA

 1261 GAGAAGAGTG GTGCAGAGAG AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT

 1321 CTTGGGAGCA GCAGGAAGCA CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG

 1381 ACAATTATTG TCTGGTATAG TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA

 1441 ACAGCATCTG TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC

 1501 TGTGGAAAGA TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT

 1561 CATTTGCACC ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT

 1621 TTGGAATCAC ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT

 1681 ACACTCCTTA ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA

 1741 ATTAGATAAA TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT

 1801 AAAATTATTC ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT

 1861 TTCTATAGTG AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC

 1921 AACCCCGAGG GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG

 1981 AGACAGATCC ATTCGATTAG TGAACGGATC TCGACGGTAT CGCGATGTCG ACGATAAGCT

 2041 TTGCAAAGAT GGATAAAGTT TTAAACAGAG AGGAATCTTT GCAGCTAATG GACCTTCTAG

 2101 GTCTTGAAAG GAGTGGGAAT TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC

 2161 ACAGTCCCCG AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG

 2221 CGCGGGGTAA ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG

 2281 GGAGAACCGT ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC

 2341 GCCAGAACAC AGGTAAGTGC CGTGTGTGGT TCCCGCGGGC CTGGCCTCTT TACGGGTTAT

 2401 GGCCCTTGCG TGCCTTGAAT TACTTCCACT GGCTGCAGTA CGTGATTCTT GATCCCGAGC

 2461 TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC

 2521 GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC

 2581 TTCGCGCCTG TCTCGCTGCT TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG

 2641 CTGCGACGCT TTTTTTCTGG CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG

 2701 GTATTTCGGT TTTTGGGGCC GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT

 2761 CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG

 2821 GCCGGCCTGC TCTGGTGCCT GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA

 2881 GGCTGGCCCG GTCGGCACCA GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG

 2941 CAGGGAGCTC AAAATGGAGG ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC

 3001 AAAGGAAAAG GGCCTTTCCG TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG

 3061 CGCCGTCCAG GCACCTCGAT TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG

 3121 GGGAGGGGTT TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC

 3181 CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT

 3241 TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG

 3301 GAATTTCGAC ATTTAAATTT AATTAATCTC GACGGTATCG GTTAACTTTT AAAAGAAAAG

 3361 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC

 3421 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCCGATC ACGAGACTAG

 3481 CCTCGAGGTT TAAACTACGG GATCTTCGAA GGCCTAAGCT TACGCGCGCG TCCTAGCGCT

 3541 ACCGGTCGCC ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT

 3601 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG

 3661 CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT

 3721 GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC

 3781 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA

 3841 GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA

 3901 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA

 3961 CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA

 4021 CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG

 4081 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT

 4141 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG

 4201 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA

 4261 GCTGTACAAG TCCGGACTCA GATCTCGATA GCTAGTAGCT AGCTAGCTAG TCGAGCTCAA

 4321 GCTTCGGGGA CTAGTCATAT GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA

 4381 CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT

 4441 TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT

 4501 TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG

 4561 TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG

 4621 GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC

 4681 GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC

 4741 TGACGTCCTT TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT

 4801 TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG

 4861 CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG

 4921 CCGCCTCCCC GCATCGGTAC GTATGGCCAG GTACCTTTAA GACCAATGAC TTACAAGGCA

 4981 GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC

 5041 CAACGAAGAC AAGATGGGAT CAATTCACCA TGCTAGTGGA TCCCCCTCGA GTTTACCACT

 5101 CCCTATCAGT GATAGAGAAA AGTGAAAGTC GAGTTTACCA CTCCCTATCA GTGATAGAGA

 5161 AAAGTGAAAG TCGAGTTTAC CACTCCCTAT CAGTGATAGA GAAAAGTGAA AGTCGAGTTT

 5221 ACCACTCCCT ATCAGTGATA GAGAAAAGTG AAAGTCGAGT TTACCACTCC CTATCAGTGA

 5281 TAGAGAAAAG TGAAAGTCGA GTTTACCACT CCCTATCAGT GATAGAGAAA AGTGAAAGTC

 5341 GAGTTTACCA CTCCCTATCA GTGATAGAGA AAAGTGAAAG TCGGGGCTGC AGGAATTCGA

 5401 ACGCTGACGT CATCAACCCG CTCCAAGGAA TCGCGGGCCC AGTGTCACTA GGCGGGAACA

 5461 CCCAGCGCGC GTGCGCCCTG GCAGGAAGAT GGCTGTGAGG GACAGGGGAG TGGCGCCCTG

 5521 CAATATTTGC ATGTCGCTAT GTGTTCTGGG AAATCACCAT AAACGTGAAA TGTCTTTGGA

 5581 TTTGGGAATC TTATAAGTTC TGTATGAGAC CACAGATCTC CAAGCTTATC GATACCGTCG

 5641 CATGGGAATA ACTTCGTATA GCATACATTA TACGAAGTTA TGCTGCTTTT TGCTTGTACT

 5701 GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA

 5761 CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG

 5821 TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC

 5881 AGCATCTAGA ATTAATTCCG TGTATTCTAT AGTGTCACCT AAATCGTATG TGTATGATAC

 5941 ATAAGGTTAT GTATTAATTG TAGCCGCGTT CTAACGACAA TATGTACAAG CCTAATTGTG

 6001 TAGCATCTGG CTTACTGAAG CAGACCCTAT CATCTCTCTC GTAAACTGCC GTCAGAGTCG

 6061 GTTTGGTTGG ACGAACCTTC TGAGTTTCTG GTAACGCCGT CCCGCACCCG GAAATGGTCA

 6121 GCGAACCAAT CAGCAGGGTC ATCGCTAGCC AGATCCTCTA CGCCGGACGC ATCGTGGCCG

 6181 GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG

 6241 AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT ATGGTGGCAG

 6301 GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA TGCACCATTC CTTGCGGCGG

 6361 CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT AATGCAGGAG TCGCATAAGG

 6421 GAGAGCGTCG AATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG

 6481 CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC

 6541 GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA

 6601 TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC

 6661 ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC

 6721 CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC

 6781 TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC

 6841 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG

 6901 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT

 6961 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC

 7021 ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA

 7081 CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA

 7141 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT

 7201 GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT

 7261 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT

 7321 GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG

 7381 CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG

 7441 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT

 7501 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG

 7561 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG

 7621 GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG

 7681 TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA

 7741 AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT

 7801 TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT

 7861 TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT

 7921 TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG

 7981 ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA

 8041 GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT

 8101 AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG

 8161 GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG

 8221 AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC

 8281 AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA

 8341 AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT

 8401 TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA

 8461 CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT

 8521 TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG

 8581 ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT

 8641 CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG

 8701 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG

 8761 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC

 8821 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC

 8881 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG

 8941 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG

 9001 GCTTTTGCAA AAAGCTTGGA CACAAGACAG GCTTGCGAGA TATGTTTGAG AATACCACTT

 9061 TATCCCGCGT CAGGGAGAGG CAGTGCGTAA AAAGACGCGG ACTCATGTGA AATACTGGTT

 9121 TTTAGTGCGC CAGATCTCTA TAATCTCGCG CAACCTATTT TCCCCTCGAA CACTTTTTAA

 9181 GCCGTAGATA AACAGGCTGG GACACTTCAC ATGAGCGAAA AATACATCGT CACCTGGGAC

 9241 ATGTTGCAGA TCCATGCACG TAAACTCGCA AGCCGACTGA TGCCTTCTGA ACAATGGAAA

 9301 GGCATTATTG CCGTAAGCCG TGGCGGTCTG TACCGGGTGC GTTACTGGCG CGTGAACTGG

 9361 GTATTCGTCA TGTCGATACC GTTTGTATTT CCAGCTACGA TCACGACAAC CAGCGCGAGC

 9421 TTAAAGTGCT GAAACGCGCA GAAGGCGATG GCGAAGGCTT CATCGTTATT GATGACCTGG

 9481 TGGATACCGG TGGTACTGCG GTTGCGATTC GTGAAATGTA TCCAAAAGCG CACTTTGTCA

 9541 CCATCTTCGC AAAACCGGCT GGTCGTCCGC TGGTTGATGA CTATGTTGTT GATATCCCGC

 9601 AAGATACCTG GATTGAACAG CCGTGGGATA TGGGCGTCGT ATTCGTCCCG CCAATCTCCG

 9661 GTCGCTAATC TTTTCAACGC CTGGCACTGC CGGGCGTTGT TCTTTTTAAC TTCAGGCGGG

 9721 TTACAATAGT TTCCAGTAAG TATTCTGGAG GCTGCATCCA TGACACAGGC AAACCTGAGC

 9781 GAAACCCTGT TCAAACCCCG CTTTAAACAT CCTGAAACCT CGACGCTAGT CCGCCGCTTT

 9841 AATCACGGCG CACAACCGCC TGTGCAGTCG GCCCTTGATG GTAAAACCAT CCCTCACTGG

 9901 TATCGCATGA TTAACCGTCT GATGTGGATC TGGCGCGGCA TTGACCCACG CGAAATCCTC

 9961 GACGTCCAGG CACGTATTGT GATGAGCGAT GCCGAACGTA CCGACGATGA TTTATACGAT

10021 ACGGTGATTG GCTACCGTGG CGGCAACTGG ATTTATGAGT GGGCCCCGGA TCTTTGTGAA

10081 GGAACCTTAC TTCTGTGGTG TGACATAATT GGACAAACTA CCTACAGAGA TTTAAAGCTC

10141 TAAGGTAAAT ATAAAATTTT TAAGTGTATA ATGTGTTAAA CTACTGATTC TAATTGTTTG

10201 TGTATTTTAG ATTCCAACCT ATGGAACTGA TGAATGGGAG CAGTGGTGGA ATGCCTTTAA

10261 TGAGGAAAAC CTGTTTTGCT CAGAAGAAAT GCCATCTAGT GATGATGAGG CTACTGCTGA

10321 CTCTCAACAT TCTACTCCTC CAAAAAAGAA GAGAAAGGTA GAAGACCCCA AGGACTTTCC

10381 TTCAGAATTG CTAAGTTTTT TGAGTCATGC TGTGTTTAGT AATAGAACTC TTGCTTGCTT

10441 TGCTATTTAC ACCACAAAGG AAAAAGCTGC ACTGCTATAC AAGAAAATTA TGGAAAAATA

10501 TTCTGTAACC TTTATAAGTA GGCATAACAG TTATAATCAT AACATACTGT TTTTTCTTAC

10561 TCCACACAGG CATAGAGTGT CTGCTATTAA TAACTATGCT CAAAAATTGT GTACCTTTAG

10621 CTTTTTAATT TGTAAAGGGG TTAATAAGGA ATATTTGATG TATAGTGCCT TGACTAGAGA

10681 TCATAATCAG CCATACCACA TTTGTAGAGG TTTTACTTGC TTTAAAAAAC CTCCCACACC

10741 TCCCCCTGAA CCTGAAACAT AAAATGAATG CAATTGTTGT TGTTAACTTG TTTATTGCAG

10801 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT

10861 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCA

10921 ACTGGATAAC TCAAGCTAAC CAAAATCATC CCAAACTTCC CACCCCATAC CCTATTACCA

10981 CTGCCAATTA CCTAGTGGTT TCATTTACTC TAAACCTGTG ATTCCTCTGA ATTATTTTCA

11041 TTTTAAAGAA ATTGTATTTG TTAAATATGT ACTACAAACT TAGTAG

//


pLVTH其他相关慢病毒载体:

pLVX-DsRed-Monomer-N1

pLVX-PAmCherry-C1

Tet-pLKO-neo

pLVX-DsRed-Express2-C1

pLVX-mCherry-C1

pLVX-AcGFP1-N1

pLKO.1-puro

FUW-tetO-hSOX2

pLVX-IRES-Puro

pCDH-MSCV-MCS-EF1-copGFP-T2A-Puro

pLVX-EF1α-DsRed-Monomer-C1

pLVX-MetLuc

pLVX-EF1α-mCherry-C1

pLVX-EF1α-IRES-mCherry

pLVX-MetLuc   Control

pLVX-EF1α-AcGFP1-N1

pLVX-Het-2

pLVX-IRES-Neo

pSIH1-H1-CopGFP

pLentilox   3.7

pRSV-rev

pLVX-DD-AmCyan1   Reporter

pLVX-Het-1

pMDLg-pRRE

pLVX-PTuner

pLVX-PTuner-Green

pLVX-rHom-1

pLOX-CWBmi1

pLVX-TetOne-Luc

pCDH-EF1-MCS-T2A-Puro

pLVX-TetOne-Puro-Luc

pLVX-DD-AcGFP1-Actin

pLVX-DD-tdTomato   Control

pLVX-TetOne

pLVX-DD-AmCyan1    Control

pLVX-rHom-Nuc1

pLenti6.3-MCS-IRES2-EGFP

pLVX-Hom-Nuc1

pLenti6.3/V5-GW/EmGFP

pLVX-PTuner2

pLVX-TRE3G-mCherry

pLVX-CherryPicker   Control

pCDH-EF1-MCS-T2A-copGFP

pCDH-CMV-MCS-EF1-Hygro

pCDH-MCS-T2A-Puro-MSCV

pCDH1-MCS2-EF1-copGFP

pCDH-CMV-MCS-EF1-RFP-T2A-Puro

pWPXL

FUGW

pLenti6.3-EmGFP-BveI   miR

pGIPZ

pLenti6.3-MCS

pLenti6.3-BveI     miR

pSicoR

pcDNA6.3-EmGFP-NC-     II

pLVX-TRE3G-IRES

VSV-G

pLentG-KOSM

pMDLg/pRRE

pFUGW

pLVX-TRE3G-Luc     Control

pLP2

pSico

pLenti6.3-MCS-IRES2-DsRed2

pGensil-1

pcDNA6.2-EmGFP-NC-   I

FUW-tetO-hOCT4

pSico   PGK Puro

FUW

pLVX-shRNA2

pCDH-MSCV-MCS-EF1-copGFP

pLKO.1-GFP-shRNA

pLKO.1-TRC     control

pPACKH1-GAG

pMD2.G

pLKO.1-TRC

pLVX-AmCyan1-N1

FUW-tetO-hOKMS

pLKO.1-hygro

FUW-tetO-hKLF4

pCMV-dR8.2-dvpr

pLVX-AcGFP1-C1

Tet-pLKO-puro

pLVX-DsRed-Express2-N1

pLVX-mCherry-N1

pLVX-PAmCherry-N1

pLVX-IRES-ZsGreen1

pLVX-PTuner2-C

pPRIME-TREX-GFP-FF3

pLVX-DD-ZsGreen1    Control

pCDH-CMV-MCS-EF1-copGFP

pCDH-UbC-MCS-EF1-Hygro

pLOX-TERT-iresTK

pCDH-CMV-MCS-EF1-RFP

pCDH-EF1-MCS-(PGK-Puro)

pCDF1-MCS2-EF1-copGFP

pLVX-Hom-1

pLVX-shRNA1

pLVX-mCherry-Actin

pcDNA6.2-DsRed2-BsmBI   miR

pLVX-CherryPicker1

pLVX-TRE3G

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro

pCDH-CMV-MCS-EF1-copGFP-T2A-Puro

pCMV-DsRed-Express2

pLVX-TRE3G-Hom1

pLVX-IRES-mCherry

pLP1

pLVX-tdTomato-C1

pLOX-Ttag-iresTK

pLVX-Tet-On-Advanced

pCMV-dR8.91

pLVX-IRES-Hyg

pCgpv

pLVX-EF1α-AcGFP1-C1

pLVTHM

pLVX-EF1α-IRES-ZsGreen1

pPACKH1-REV

pLVX-Hom-Mem1

pLKO.1-puro-GFP-siRNA

pCDH-CMV-MCS-EF1-Puro

FUW-tetO-hMYC

pPRIME-TET-GFP-FF3

FUW-M2rtTA

pLOX-CW-CRE

pLVX-AmCyan1-C1

pLL3.7

pLVX-DsRed-Monomer-C1

pLVX-DD-tdTomato     Reporter

pLVX-ZsGreen1-C1

pLVX-CherryPicker2

pLVX-IRES-tdTomato

pLVX-TetOne-Puro

pLVX-tdTomato-N1

pLVX-rHom-Sec1

pLVX-Tight-Puro

pLVX-Het-Nuc1

pLVX-EF1α-mCherry-N1

pLVX-DD-ZsGreen1     Reporter

pLVX-EF1α-IRES-Puro

pCDH-CMV-MCS-EF1-Neo

pLVX-Het-Mem1

pCDF1-MCS2-EF1-Puro

pLP/VSVG

pLVX-TRE3G-ZsGreen1

pcDNA6.2-EmGFP-MCS1   miR

pLenti6/V5-GW/lacZ

pcDNA6.2-BsaI   miR

pLenti6.3-DsRed2-BveI   miR

pTRIPZ

psPAX2

LeGO-iC2

pcDNA6.2-DsRed2-MCS1   miR

pLKO.3G

pcDNA6.2-EmGFP-BsaI   miR

pLVX-Puro

pLEX-MCS

pSicoR   PGK Puro