北京华越洋生物
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首页    载体及质粒    pDC411
224

pDC411

pDC411

pDC411

 

编号

载体名称

北京华越洋VECT10021

pDC411

 

pDC411载体基本信息:

载体名称:

pDC411

质粒类型:

腺病毒表达载体

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

--

载体大小:

5574 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

--

备注:

克隆宿主菌:DB3.1

稳定性:

--

组成型:

组成型

病毒/非病毒:

腺病毒

 

pDC411载体质粒图谱和多克隆位点信息:

pDC411载体序列:

 

ORIGIN

        1 TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT

       61 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG

      121 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG

      181 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT

      241 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA

      301 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG

      361 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC

      421 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGNTCTAGA GAATTCAGGC CTGCTAGCAG

      481 ATCTAAGCTT GAGCTCGTCG ACTCGAAGAT CGATCTGGGC GTGGTTAAGG GTGGGAAAGA

      541 ATATATAAGG TGGGGGTCTT ATGTAGTTTT GTATCTGTTT TGCAGCAGCC GCCGCCGCCA

      601 TGAGCACCAA CTCGTTTGAT GGAAGCATTG TGAGCTCATA TTTGACAACG CGCATGCCCC

      661 CATGGGCCGG GGTGCGTCAG AATGTGATGG GCTCCAGCAT TGATGGTCGC CCCGTCCTGC

      721 CCGCAAACTC TACTACCTTG ACCTACGAGA CCGTGTCTGG AACGCCGTTG GAGACTGCAG

      781 CCTCCGCCGC CGCTTCAGCC GCTGCAGCCA CCGCCCGCGG GATTGTGACT GACTTTGCTT

      841 TCCTGAGCCC GCTTGCAAGC AGTGCAGCTT CCCGTTCATC CGCCCGCGAT GACAAGTTGA

      901 CGGCTCTTTT GGCACAATTG GATTCTTTGA CCCGGGAACT TAATGTCGTT TCTCAGCAGC

      961 TGTTGGATCT GCGCCAGCAG GTTTCTGCCC TGAAGGCTTC CTCCCCTCCC AATGCGGTTT

     1021 AAAACATAAA TAAAAAACCA GACTCTGTTT GGATTTGGAT CAAGCAAGTG TCTTGCTGTC

     1081 TTTATTTAGG GGTTTTGCGC GCGCGGTAGG CCCGGGACCA GCGGTCTCGG TCGTTGAGGG

     1141 TCCTGTGTAT TTTTTCCAGG ACGTGGTAAA GGTGACTCTG GATGTTCAGA TACATGGGCA

     1201 TAAGCCCGTC TCTGGGGTGG AGGTAGCACC ACTGCAGAGC TTCATGCTGC GGGGTGGTGT

     1261 TGTAGATGAT CCAGTCGTAG CAGGAGCGCT GGGCGTGGTG CCTAAAAATG TCTTTCAGTA

     1321 GCAAGCTGAT TGCCAGGGGC AGGCCCTTGG TGTAAGTGTT TACAAAGCGG TTAAGCTGGG

     1381 ATGGGTGCAT ACGTGGGGAT ATGAGATGCA TCTTGGACTG TATTTTTAGG TTGGCTATGT

     1441 TCCCAGCCAT ATCCCTCCGG GGATTCATGT TGTGCAGAAC CACCAGCACA GTGTATCCGG

     1501 TGCACTTGGG AAATTTGTCA TGTAGCTTAG AAGGAAATGC GTGGAAGAAC TTGGAGACGC

     1561 CCTTGTGACC TCCAAGATTT TCCATGCATT CGTCCATAAT GATGGCAATG GGCCCACGGG

     1621 CGGCGGCCTG GGCGAAGATA TTTCTGGGAT CACTAACGTC ATAGTTGTGT TCCAGGATGA

     1681 GATCGTCATA GGCCATTTTT ACAAAGCGCG GGCGGAGGGT GCCAGACTGC GGTATAATGG

     1741 TTCCATCCGG CCCAGGGGCG TAGTTACCCT CACAGATTTG CATTTCCCAC GCTTTGAGTT

     1801 CAGATGGGGG GATCATGTCT ACCTGCGGGG CGATGAAGAA AACGGTTTCC GGGGTAGGGG

     1861 AGATCAGCTG GGAAGAAAGC AGGTTCCTGA GCAGCTGCGA CTTACCGCAG CCGGTGGGCC

     1921 CGTAAATCAC ACCTATTACC GGGTGCAACT GGTAGTTAAG AGAGCTGCAG CTGCCGTCAT

     1981 CCCTGAGCAG GGGGGCCACT TCGTTAAGCA TGTCCCTGAC TCGCATGTTT TCCCTGACCA

     2041 AATCCGCCAG AAGGCGCTCG CCGCCCAGCG ATAGCAGTTC TTGCAAGGAA GCAAAGTTTT

     2101 TCAACGGTTT GAGACCGTCC GCCGTAGGCA TGCTTTTGAG CGTTTGACCA AGCAGTTCCA

     2161 GGCGGTCCCA CAGCTCGGTC ACCTGCTCTA CGGCATCTCG ATCCAGCATA TCTCCTCGTT

     2221 TCGCGGGTTG GGGCGGCTTT CGCTGTACGG CAGTAGTCGG TGCTCGTCCA GACGGGCCAG

     2281 GGTCATGTCT TTCCACGGGC GCAGGGTCCT CGTCAGCGTA GTCTGGGTCA CGGTGAAGGG

     2341 GTGCGCTCCG GGCTGCGCGC TGGCCAGGGT GCGCTTGAGG CTGGTCCTGC TGGTGCTGAA

     2401 GCGCTGCCGG TCTTCGCCCT GCGCGTCGGC CAGGTAGCAT TTGACCATGG TGTCATAGTC

     2461 CAGCCCCTCC GCGGCGTGGC CCTTGGCGCG CAGCTTGCCC TTGGAGGAGG CGCCGCACGA

     2521 GGGGCAGTGC AGACTTTTGA GGGCGTAGAG CTTGGGCGCG AGAAATACCG ATTCCGGGGA

     2581 GTAGGCATCC GCGCCGCAGG CCCCGCAGAC GGTCTCGCAT TCCACGAGCC AGGTGAGCTC

     2641 TGGCCGTTCG GGGTCAAAAA CCAGGTTTCC CCCATGCTTT TTGATGCGTT TCTTACCTCT

     2701 GGTTTCCATG AGCCGGTGTC CACGCTCGGT GACGAAAAGG CTGTCCGTGT CCCCGTATAC

     2761 AGACTTGAGA GGCCTGTCCT CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT

     2821 TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC

     2881 AACTCGTAGG ACAGGTGCCG GCAGCGCTCT GGGTCATTTT CGGCGAGGAC CGCTTTCGCT

     2941 GGAGCGCGAC GATGATCGGC CTGTCGCTTG CGGTATTCGG AATCTTGCAC GCCCTCGCTC

     3001 AAGCCTTCGT CACTGGTCCC GCCACCAAAC GTTTCGGCGA GAAGCAGGCC ATTATCGCCG

     3061 GCATGGCGGC CCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG

     3121 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG

     3181 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG

     3241 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC

     3301 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC

     3361 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC

     3421 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT

     3481 CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG

     3541 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT

     3601 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG

     3661 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT

     3721 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC

     3781 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA

     3841 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG

     3901 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA

     3961 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA

     4021 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA

     4081 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC

     4141 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA

     4201 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA

     4261 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT

     4321 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG

     4381 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC

     4441 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG

     4501 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG

     4561 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT

     4621 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT

     4681 CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC

     4741 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC

     4801 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG

     4861 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA

     4921 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA

     4981 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC

     5041 CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA

     5101 ATAGGCGTAT CACTCTAGGC AAAATAGCAC CCTCCCGCTC CAGAACAACA TACAGCGCTT

     5161 CACAGCGGCA GCCTAACAGT CAGCCTTACC AGTAAAAAAG AAAACCTATT AAAAAAACAC

     5221 CACTCGACAC GGCACCAGCT CAATCAGTCA CAGTGTAAAA AAGGGCCAAG TGCAGAGCGA

     5281 GTATATATAG GACTAAAAAA TGACGTAACG GTTAAAGTCC ACAAAAAACA CCCAGAAAAC

     5341 CGCACGCGAA CCTACGCCCA GAAACGAAAG CCAAAAAACC CACAACTTCC TCAAATCGTC

     5401 ACTTCCGTTT TCCCACGTTA CGTAACTTCC CATTTTAAGA AAACTACAAT TCCCAACACA

     5461 TACAAGTTAC TCCGCCCTAA AACCTACGTC ACCCGCCCCG TTCCCACGCC CCGCGCCACG

     5521 TCACAAACTC CACCCCCTCA TTATCATATT GGCTTCAATC CAAAATAAGG TATA

//

 

pDC411其他腺病毒表达载体:

pNTAP-Shuttle-B

pAAV-CAG-RFP

pAAV-CaMKIIa-EGFP

pCTAP-Shuttle-A

pAAV-CMV-Rluc

pNTAP-Shuttle-A

pAAV-CAG-GFP

pBHGloxdelE13cre

pCTAP-Shuttle-C

pDC511

pDC515

pDC312

pAdTrack-CMV

pAAV-IRES-hrGFP

pShuttle-IRES-hrGFP-1

pShuttle-CMV-EGFP-C

pacAd5 9.2-100

pacAd5 CMV-GFP

pAAV-Syn-Rluc

pCMV-MCS

pShuttle-IRES-hrGFP-2

pShuttle-CMV

pAAV-Syn-Rluc

pAAV-hSyn-RFP

pAAV-CMV-iRFP

pAAV-UbC

pDC415

pAAV-CAG-Fluc

pAAV-LacZ

pNTAP-Shuttle-B

pDC316

pDC516

pAAV-CA

pDC416

pNTAP-Shuttle-C

pDC311

pDC512

pAAV-hrGFP

pDC411

pAAV-RC

pAdTrack

pacAd5 CMVK-NpA

pAdEasy-1

pDC315

pAAV-MCS

pShuttle

pHelper

pBApo-CMV-neo

pShuttle-CMV-lacZ

pBApo-CMV

pAAV-TRE-Syn-Fluc

pBApo-CMV-Pur

pAAV-minCMV-mCherry