北京华越洋生物
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225

pDC512

pDC512

pDC512

 

编号

载体名称

北京华越洋VECT10020

pDC512

 

pDC512载体基本信息:

载体名称:

pDC512

质粒类型:

腺病毒表达载体

高拷贝/低拷贝:

--

启动子:

--

克隆方法:

--

载体大小:

3277 bp

5' 测序引物及序列:

--

3' 测序引物及序列:

--

载体标签:

--

载体抗性:

氨苄青霉素(Ampicillin

筛选标记:

--

备注:

克隆宿主菌:DB3.1

稳定性:

--

组成型:

组成型

病毒/非病毒:

腺病毒

 

pDC512载体质粒图谱和多克隆位点信息

pDC512载体序列:

 

ORIGIN

        1 TTCATCAATA ATATACCTTA TTTTGGATTG AAGCCAATAT GATAATGAGG GGGTGGAGTT

       61 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG

      121 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG

      181 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT

      241 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA

      301 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG

      361 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC

      421 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCTCTAGA GTCGACGAGC TCAAGCTTAG

      481 ATCTGCTAGC AGGCCTGAAT TCGGATCCGA AGTTCCTATT CTTACTAGAG TATAGGAACT

      541 TCGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG

      601 CCGGCAGCGC TCTGGGTCAT TTTCGGCGAG GACCGCTTTC GCTGGAGCGC GACGATGATC

      661 GGCCTGTCGC TTGCGGTATT CGGAATCTTG CACGCCCTCG CTCAAGCCTT CGTCACTGGT

      721 CCCGCCACCA AACGTTTCGG CGAGAAGCAG GCCATTATCG CCGGCATGGC GGCCCTGCAT

      781 TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC

      841 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA

      901 AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA

      961 AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG

     1021 CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG

     1081 ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT

     1141 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT

     1201 TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC

     1261 TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT

     1321 GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT

     1381 AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC

     1441 TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA

     1501 AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT

     1561 TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT

     1621 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA

     1681 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA

     1741 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC

     1801 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT

     1861 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC

     1921 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT

     1981 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA

     2041 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG

     2101 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT

     2161 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC

     2221 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT

     2281 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC

     2341 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAACACG GGATAATACC

     2401 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA

     2461 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC

     2521 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA

     2581 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT

     2641 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA

     2701 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT

     2761 GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACTCTA

     2821 GGCAAAATAG CACCCTCCCG CTCCAGAACA ACATACAGCG CTTCACAGCG GCAGCCTAAC

     2881 AGTCAGCCTT ACCAGTAAAA AAGAAAACCT ATTAAAAAAA CACCACTCGA CACGGCACCA

     2941 GCTCAATCAG TCACAGTGTA AAAAAGGGCC AAGTGCAGAG CGAGTATATA TAGGACTAAA

     3001 AAATGACGTA ACGGTTAAAG TCCACAAAAA ACACCCAGAA AACCGCACGC GAACCTACGC

     3061 CCAGAAACGA AAGCCAAAAA ACCCACAACT TCCTCAAATC GTCACTTCCG TTTTCCCACG

     3121 TTACGTAACT TCCCATTTTA AGAAAACTAC AATTCCCAAC ACATACAAGT TACTCCGCCC

     3181 TAAAACCTAC GTCACCCGCC CCGTTCCCAC GCCCCGCGCC ACGTCACAAA CTCCACCCCC

     3241 TCATTATCAT ATTGGCTTCA ATCCAAAATA AGGTATA

//

 

pDC512其他腺病毒表达载体:

pNTAP-Shuttle-B

pAAV-CAG-RFP

pAAV-CaMKIIa-EGFP

pCTAP-Shuttle-A

pAAV-CMV-Rluc

pNTAP-Shuttle-A

pAAV-CAG-GFP

pBHGloxdelE13cre

pCTAP-Shuttle-C

pDC511

pDC515

pDC312

pAdTrack-CMV

pAAV-IRES-hrGFP

pShuttle-IRES-hrGFP-1

pShuttle-CMV-EGFP-C

pacAd5 9.2-100

pacAd5 CMV-GFP

pAAV-Syn-Rluc

pCMV-MCS

pShuttle-IRES-hrGFP-2

pShuttle-CMV

pAAV-Syn-Rluc

pAAV-hSyn-RFP

pAAV-CMV-iRFP

pAAV-UbC

pDC415

pAAV-CAG-Fluc

pAAV-LacZ

pNTAP-Shuttle-B

pDC316

pDC516

pAAV-CA

pDC416

pNTAP-Shuttle-C

pDC311

pDC512

pAAV-hrGFP

pDC411

pAAV-RC

pAdTrack

pacAd5 CMVK-NpA

pAdEasy-1

pDC315

pAAV-MCS

pShuttle

pHelper

pBApo-CMV-neo

pShuttle-CMV-lacZ

pBApo-CMV

pAAV-TRE-Syn-Fluc

pBApo-CMV-Pur

pAAV-minCMV-mCherry