pAAV-UbC
pAAV-UbC
编号 | 载体名称 |
北京华越洋VECT10041 | pAAV-UbC |
pAAV-UbC载体基本信息：
载体名称: | pAAV-UbC |
质粒类型: | 腺病毒载体；RNAi载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | UbC |
载体大小: | 5185 bp |
5' 测序引物及序列: | pCAG fwd primer 5’CAACGTGCTGGTTATTGTG 3’ |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 或 NEB Stble |
宿主细胞（系）: | 常规哺乳动物细胞系 |
备注: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 腺病毒 |
pAAV-UbC载体质粒图谱和多克隆位点信息：
pAAV-UbC载体序列：
ORIGIN
1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCG GCCTCCGCGC CGGGTTTTGG CGCCTCCCGC
181 GGGCGCCCCC CTCCTCACGG CGAGCGCTGC CACGTCAGAC GAAGGGCGCA GCGAGCGTCC
241 TGATCCTTCC GCCCGGACGC TCAGGACAGC GGCCCGCTGC TCATAAGACT CGGCCTTAGA
301 ACCCCAGTAT CAGCAGAAGG ACATTTTAGG ACGGGACTTG GGTGACTCTA GGGCACTGGT
361 TTTCTTTCCA GAGAGCGGAA CAGGCGAGGA AAAGTAGTCC CTTCTCGGCG ATTCTGCGGA
421 GGGATCTCCG TGGGGCGGTG AACGCCGATG ATTATATAAG GACGCGCCGG GTGTGGCACA
481 GCTAGTTCCG TCGCAGCCGG GATTTGGGTC GCGGTTCTTG TTTGTGGATC GCTGTGATCG
541 TCACTTGGTG AGTAGCGGGC TGCTGGGCTG GCCGGGGCTT TCGTGGCCGC CGGGCCGCTC
601 GGTGGGACGG AAGCGTGTGG AGAGACCGCC AAGGGCTGTA GTCTGGGTCC GCGAGCAAGG
661 TTGCCCTGAA CTGGGGGTTG GGGGGAGCGC AGCAAAATGG CGGCTGTTCC CGAGTCTTGA
721 ATAGAACCTT CGCTAATGCG GGAAAGCTCT TATTCGGGTG AGATGGGCTG GGGCACCATC
781 TGGGGACCCT GACGTGAAGT TTGTCACTGA CTGGAGAACT CGGTTTGTCG TCTGTTGCGG
841 GGGCGGCAGT TATGGCGGTG CCGTTGGGCA GTGCACCCGT ACCTTTGGGA GCGCGCGCCC
901 TCGTCGTGTC GTGACGTCAC CCGTTCTGTT GGCTTATAAT GCAGGGTGGG GCCACCTGCC
961 GGTAGGTGTG CGGTAGGCTT TTCTCCGTCG CAGGACGCAG GGTTCGGGCC TAGGGTAGGC
1021 TCTCCTGAAT CGACAGGCGC CGGACCTCTG GTGAGGGGAG GGATAAGTGA GGCGTCAGTT
1081 TCTTTGGTCG GTTTTATGTA CCTATCTTCT TAAGTAGCTG AAGCTCCGGT TTTGAACTAT
1141 GCGCTCGGGG TTGGCGAGTG TGTTTTGTGA AGTTTTTTAG GCACCTTTTG AAATGTAATC
1201 ATTTGGGTCA ATATGTAATT TTCAGTGTTA GACTAGTAAA TTGTCCGCTA AATTCTGGCC
1261 GTTTTTGGCT TTTTTGTTAG ACGAAGCTAA GGCGCGCCTG AGAACTTCAG GGTGAGTTTG
1321 GGGACCCTTG ATTGTTCTTT CTTTTTCGCT ATTGTAAAAT TCATGTTATA TGGAGGGGGC
1381 AAAGTTTTCA GGGTGTTGTT TAGAATGGGA AGATGTCCCT TGTATCACCA TGCATGGACC
1441 CTCATGATAA TTTTGTTTCT TTCACTTTCT ACTCTGTTGA CAACCATTGT CTCCTCTTAT
1501 TTTCTTTTCA TTTTCTGTAA CTTTTTCGTT AAACTTTAGC TTGCATTTGT AACGAATCTA
1561 GACAATCAGG GTATATTATA TTGTACTTCA GCACAGTTTT AGAGAACAAT TGTTATAATT
1621 AAATGATAAG GTAGAATATT TCTGCATATA AATTCTGGCT GGCGTGGAAA TATTCTTATT
1681 GGTAGAAACA ACTACATCCT GGTCATCATC CTGCCTTTCT CTTTATGGTT ACAATGATAT
1741 ACACTGTTTG AGATGAGGAT AAAATACTCT GAGTCCAAAC CGGGCCCCTC TGCTAACCAT
1801 GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT
1861 CATCAAGCTT GGATCCGAAT TCGCTAGCGA TATCTCGAGT CTAGAGTTAA CGGCCATATT
1921 GGCCAATCTA CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG
1981 TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG
2041 ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG
2101 TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG
2161 CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC
2221 CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT
2281 GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA
2341 TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC
2401 CTGTCCTTCT GATTTTGTAG GTAACCACGT GCGGACCGAG CGGCCGCAGG AACCCCTAGT
2461 GATGGAGTTG GCCACTCCCT CTCTGCGCGC TCGCTCGCTC ACTGAGGCCG GGCGACCAAA
2521 GGTCGCCCGA CGCCCGGGCT TTGCCCGGGC GGCCTCAGTG AGCGAGCGAG CGCGCAGCTG
2581 CCTGCAGGGG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG
2641 CATACGTCAA AGCAACCATA GTACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG
2701 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT
2761 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG
2821 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG
2881 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG
2941 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC
3001 TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT
3061 GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGTT TACAATTTTA
3121 TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG
3181 CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA
3241 GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC
3301 GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG
3361 GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA
3421 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT
3481 CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC
3541 TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA
3601 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT
3661 AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT
3721 CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC AAGAGCAACT CGGTCGCCGC
3781 ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG
3841 GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG
3901 GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC
3961 ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA
4021 AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA CAACGTTGCG CAAACTATTA
4081 ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT
4141 AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA
4201 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG
4261 CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT
4321 AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT
4381 TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG
4441 AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA
4501 GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA
4561 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA
4621 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT
4681 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA
4741 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT
4801 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG
4861 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG
4921 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA
4981 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT
5041 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG
5101 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
5161 TTTTGCTGGC CTTTTGCTCA CATGT
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pAAV-UbC其他腺病毒表达载体：
