pAAV-CAG-Fluc

pAAV-CAG-Fluc

pAAV-CAG-Fluc载体基本信息：

pAAV-CAG-Fluc载体质粒图谱和多克隆位点信息：

pAAV-CAG-Fluc载体简介：

This AAV is a fLuc construct driven by the ubiquitous CAG promoter.

The roughly 1.8Kb CAG promoter (also known as CBA promoter or CAGGS promoter) is a strong synthetic promoter frequently used to drive high levels of gene expression in mammalian cells. The CAG promoter is composed of the following regulatory elements: (C) cytomegalovirus (CMV) early enhancer element; (A) the promoter region, the first exon, and the first intron of chicken beta-Actin gene, and (G) the splice acceptor of the rabbit beta-Globin gene. Like the EF1a promoter, the CAG promoter is commonly used as an alternative for the CMV promoter, from which the expression is decreased due to methylation/silencing. In many cell types tested, the CAG and EF1a promoters give much higher levels of expression than other commonly used cellular promoters such as the UBC and PGK promoters.

Firefly luciferase (fLuc) is an oxidative enzyme used in bioluminescence and is distinct from a photoprotein. In the luciferase reaction, light is emitted when luciferase acts on the appropriate luciferin substrate. Photon emission can be detected by a light-sensitive apparatus such as a luminometer or modified optical microscope. Firefly luciferase is commonly used as a reporter to assess the transcriptional activity in cells.

pAAV-CAG-Fluc载体序列：

ORIGIN     

        1 GGGGGGGGGG GGGGGGGGTT GGCCACTCCC TCTCTGCGCG CTCGCTCGCT CACTGAGGCC

       61 GGGCGACCAA AGGTCGCCCG ACGCCCGGGC TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA

      121 GCGCGCAGAG AGGGAGTGGC CAACTCCATC ACTAGGGGTT CCTAGATCTA GGATCACGCG

      181 TTCTAGTCCT AGGGATGTAC CATTGACGTC AATAATGACG TATGTTCCCA TAGTAACGCC

      241 AATAGGGACT TTCCATTGAC GTCAATGGGT GGAGTATTTA CGGTAAACTG CCCACTTGGC

      301 AGTACATCAA GTGTATCATA TGCCAAGTAC GCCCCCTATT GACGTCAATG ACGGTAAATG

      361 GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC TTTCCTACTT GGCAGTACAT

      421 CTACGTATTA GTCATCGCTA TTACCATGGT CGAGGTGAGC CCCACGTTCT GCTTCACTCT

      481 CCCCATCTCC CCCCCCTCCC CACCCCCAAT TTTGTATTTA TTTATTTTTT AATTATTTTG

      541 TGCAGCGATG GGGGCGGGGG GGGGGGGGGG GCGCGCGCCA GGCGGGGCGG GGCGGGGCGA

      601 GGGGCGGGGC GGGGCGAGGC GGAGAGGTGC GGCGGCAGCC AATCAGAGCG GCGCGCTCCG

      661 AAAGTTTCCT TTTATGGCGA GGCGGCGGCG GCGGCGGCCC TATAAAAAGC GAAGCGCGCG

      721 GCGGGCGGGA GTCGCTGCGC GCTGCCTTCG CCCCGTGCCC CGCTCCGCCG CCGCCTCGCG

      781 CCGCCCGCCC CGGCTCTGAC TGACCGCGTT ACTCCCACAG GTGAGCGGGC GGGACGGCCC

      841 TTCTCCTCCG GGCTGTAATT AGCGCTTGGT TTAATGACGG CTTGTTTCTT TTCTGTGGCT

      901 GCGTGAAAGC CTTGAGGGGC TCCGGGAGGG CCCTTTGTGC GGGGGGAGCG GCTCGGGGCT

      961 GTCCGCGGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC GGCTTCTGGC

     1021 GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC

     1081 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTGGATC

     1141 TAGTCGATTC GAATTCATGG AAGACGCCAA AAACATAAAG AAAGGCCCGG CGCCATTCTA

     1201 TCCGCTAGAG GATGGAACCG CTGGAGAGCA ACTGCATAAG GCTATGAAGA GATACGCCCT

     1261 GGTTCCTGGA ACAATTGCTT TTACAGATGC ACATATCGAG GTGAACATCA CGTACGCGGA

     1321 ATACTTCGAA ATGTCCGTTC GGTTGGCAGA AGCTATGAAA CGATATGGGC TGAATACAAA

     1381 TCACAGAATC GTCGTATGCA GTGAAAACTC TCTTCAATTC TTTATGCCGG TGTTGGGCGC

     1441 GTTATTTATC GGAGTTGCAG TTGCGCCCGC GAACGACATT TATAATGAAC GTGAATTGCT

     1501 CAACAGTATG AACATTTCGC AGCCTACCGT AGTGTTTGTT TCCAAAAAGG GGTTGCAAAA

     1561 AATTTTGAAC GTGCAAAAAA AATTACCAAT AATCCAGAAA ATTATTATCA TGGATTCTAA

     1621 AACGGATTAC CAGGGATTTC AGTCGATGTA CACGTTCGTC ACATCTCATC TACCTCCCGG

     1681 TTTTAATGAA TACGATTTTG TACCAGAGTC CTTTGATCGT GACAAAACAA TTGCACTGAT

     1741 AATGAACTCC TCTGGATCTA CTGGGTTACC TAAGGGTGTG GCCCTTCCGC ATAGAACTGC

     1801 CTGCGTCAGA TTCTCGCATG CCAGAGATCC TATTTTTGGC AATCAAATCA TTCCGGATAC

     1861 TGCGATTTTA AGTGTTGTTC CATTCCATCA CGGTTTTGGA ATGTTTACTA CACTCGGATA

     1921 TTTGATATGT GGATTTCGAG TCGTCTTAAT GTATAGATTT GAAGAAGAGC TGTTTTTACG

     1981 ATCCCTTCAG GATTACAAAA TTCAAAGTGC GTTGCTAGTA CCAACCCTAT TTTCATTCTT

     2041 CGCCAAAAGC ACTCTGATTG ACAAATACGA TTTATCTAAT TTACACGAAA TTGCTTCTGG

     2101 GGGCGCACCT CTTTCGAAAG AAGTCGGGGA AGCGGTTGCA AAACGCTTCC ATCTTCCAGG

     2161 GATACGACAA GGATATGGGC TCACTGAGAC TACATCAGCT ATTCTGATTA CACCCGAGGG

     2221 GGATGATAAA CCGGGCGCGG TCGGTAAAGT TGTTCCATTT TTTGAAGCGA AGGTTGTGGA

     2281 TCTGGATACC GGGAAAACGC TGGGCGTTAA TCAGAGAGGC GAATTATGTG TCAGAGGACC

     2341 TATGATTATG TCCGGTTATG TAAACAATCC GGAAGCGACC AACGCCTTGA TTGACAAGGA

     2401 TGGATGGCTA CATTCTGGAG ACATAGCTTA CTGGGACGAA GACGAACACT TCTTCATAGT

     2461 TGACCGCTTG AAGTCTTTAA TTAAATACAA AGGATACCAG GTGGCCCCCG CTGAATTGGA

     2521 GTCGATATTG TTACAACACC CCAACATCTT CGACGCGGGC GTGGCAGGTC TTCCCGACGA

     2581 TGACGCCGGT GAACTTCCCG CCGCCGTTGT TGTTTTGGAG CACGGAAAGA CGATGACGGA

     2641 AAAAGAGATC GTGGATTACG TCGCCAGTCA AGTAACAACC GCGAAAAAGT TGCGCGGAGG

     2701 AGTTGTGTTT GTGGACGAAG TACCGAAAGG TCTTACCGGA AAACTCGACG CAAGAAAAAT

     2761 CAGAGAGATC CTCATAAAGG CCAAGAAGGG CGGAAAGTCC AAATTGCTCG AGGGCAGAGG

     2821 AAGTCTTCTA ACATGCGGTG ACGTGGAGGA GAATCCCGGC CCTGCACCGG GATCCATGTC

     2881 CAGATTAGAT AAAAGTAAAG TGATTAACAG CGCATTAGAG CTGCTTAATG AGGTCGGAAT

     2941 CGAAGGTTTA ACAACCCGTA AACTCGCCCA GAAGCTAGGT GTAGAGCAGC CTACATTGTA

     3001 TTGGCACGTG CGCAACAAGC AGACTCTTAT GAACATGCTT TCAGAGGCAA TACTGGCGAA

     3061 GCATCACACC CGTTCAGCAC CGTTACCGAC TGAGAGTTGG CAGCAGTTTC TCCAGGAAAA

     3121 TGCTCTGAGT TTCCGTAAAG CATTACTGGT CCATCGTGAT GGAGCCCGAT TGCATATAGG

     3181 GACCTCTCCT ACGCCCCCCC AGTTTGAACA AGCAGAGGCG CAACTACGCT GTCTATGCGA

     3241 TGCAGGGTTT TCGGTCGAGG AGGCTCTTTT CATTCTGCAA TCTATCAGCC ATTTTACGTT

     3301 GGGTGCAGTA TTAGAGGAGC AAGCAACAAA CCAGATAGAA AATAATCATG TGATAGACGC

     3361 TGCACCACCA TTATTACAAG AGGCATTTAA TATTCAGGCG AGAACCTCTG CTGAAATGGC

     3421 CTTCCATTTC GGGCTGAAAT CATTAATATT TGGATTTTCT GCACAGTTAG ATGAAAAAAA

     3481 GCATACACCC ATTGAGGATG GTAATAAACC AAAAAAGAAG AGAAAGCTAG CAGTGTCAGT

     3541 GACATTTGAA GATGTGGCTG TGCTCTTTAC TCGGGACGAG TGGAAGAAGC TGGATCTGTC

     3601 TCAGAGAAGC CTGTACCGTG AGGTGATGCT GGAGAATTAC AGCAACCTGG CCTCCATGGC

     3661 AGGATTCCTG TTTACCAAAC CAAAGGTGAT CTCCCTGTTG CAGCAAGGAG AGGACCCGTA

     3721 TCTCGAGGGC AGAGGAAGTC TTCTAACATG CGGTGACGTG GAGGAGAATC CCGGCCCTGC

     3781 ACCGGGATCC ATGGTGAGTG TGATTAAACC AGAAATGAAG ATGAGGTACT ACATGGACGG

     3841 CTCCGTCAAT GGGCATGAGT TCACAATTGA AGGTGAAGGC ACAGGCAGAC CTTACGAGGG

     3901 ACATCAAGAG ATGACACTAC GCGTCACAAT GGCCAAGGGC GGGCCAATGC CTTTCGCGTT

     3961 TGACTTAGTG TCACACGTGT TCTGTTACGG CCACAGACCT TTTACTAAAT ATCCAGAAGA

     4021 GATACCAGAC TATTTCAAAC AAGCATTTCC TGAAGGCCTG TCATGGGAAA GGTCGTTGGA

     4081 GTTCGAAGAT GGTGGGTCCG CTTCAGTCAG TGCGCATATA AGCCTTAGAG GAAACACCTT

     4141 CTACCACAAA TCCAAATTTA CTGGGGTTAA CTTTCCTGCC GATGGTCCTA TCATGCAAAA

     4201 CCAAAGTGTT GATTGGGAGC CATCAACCGA GAAAATTACT GCCAGCGACG GAGTTCTGAA

     4261 GGGTGATGTT ACGATGTACC TAAAACTTGA AGGAGGCGGC AATCACAAAT GCCAATTCAA

     4321 GACTACTTAC AAGGCGGCAA AAAAGATTCT TAAAATGCCA GGAAGCCATT ACATCAGCCA

     4381 TCGCCTCGTC AGGAAAACCG AAGGCAACAT TACTGAGCTG GTAGAAGATG CAGTAGCTCA

     4441 TTCCTGATGT ACAAGTAAAG CGGCCGCAAG CTTATCGATA ATCAACCTCT GGATTACAAA

     4501 ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC

     4561 GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC

     4621 TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT

     4681 GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC

     4741 TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC

     4801 GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG

     4861 GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT

     4921 CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC

     4981 CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT

     5041 CGGATCTCCC TTTGGGCCGC CTCCCCGCCT GATCGATACC GTCGACTAGA GCTCGCTGAT

     5101 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT

     5161 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT

     5221 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG

     5281 GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGAGAG ATCTGAGGAA CCCCTAGTGA

     5341 TGGAGTTGGC CACTCCCTCT CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC

     5401 CCGGGCGTCG GGCGACCTTT GGTCGCCCGG CCTCAGTGAG CGAGCGAGCG CGCAGAGAGG

     5461 GAGTGGCCAA CCCCCCCCCC CCCCCCCCTG CAGCCTGGCG TAATAGCGAA GAGGCCCGCA

     5521 CCGATCGCCC TTCCCAACAG TTGCGTAGCC TGAATGGCGA ATGGCGCGAC GCGCCCTGTA

     5581 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA

     5641 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT

     5701 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC

     5761 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT

     5821 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC

     5881 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC

     5941 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTTA

     6001 ACAAAATATT AACGTTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG

     6061 TATTTCACAC CGCATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG

     6121 CCAGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC

     6181 ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC

     6241 GTCATCACCG AAACGCGCGA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA

     6301 TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG

     6361 AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA

     6421 ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG

     6481 TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC

     6541 GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT

     6601 GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT

     6661 GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA

     6721 GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC

     6781 AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT

     6841 GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC

     6901 CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT

     6961 GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC

     7021 GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA

     7081 CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG

     7141 GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT

     7201 GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC

     7261 TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA

     7321 ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT

     7381 TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA

     7441 GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC

     7501 TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT

     7561 TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC

     7621 GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC

     7681 TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG

     7741 CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG

     7801 GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA

     7861 ACTGAGATAC CTACAGCGTG AGCATTGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC

     7921 GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG

     7981 GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG

     8041 ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT

     8101 TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC

     8161 TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG

     8221 AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC

     8281 GCCTCTCCCC GCGCGTTGGC CGATTCATTA ATGCAGGCTG CAG

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pAAV-CAG-Fluc其他腺病毒表达载体：