pMETαB
pMETαB
pMETαB
编号 | 载体名称 |
北京华越洋生物VECT3060 | pMETαB |
pMETαB载体基本信息
| 出品公司: | -- |
|---|---|
| 载体名称: | pMETαB |
| 质粒类型: | 毕赤酵母蛋白表达载体（methanoloica酵母） |
| 表达水平: | 高拷贝 |
| 诱导方法: | 甲醇诱导 |
| 启动子: | AUG1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8048 bp |
| 5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´; alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´ |
| 3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
| 载体标签: | C-V5， C-His，N-alpha分泌因子 |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 含有alpha factor酵母分泌信号肽，利用甲醇诱导蛋白在methanoloica酵母中分泌表达蛋白。 |
| 产品目录号: | -- |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pMETαB载体质粒图谱和多克隆位点信息
pMETαB载体序列
LOCUS pMET\alpha\B 8048 bp DNA circular 1-AUG-2013 DEFINITION pMET alpha B. SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT ORIGDB|GenBank COMMENT VNTNAME|pMET alpha B| FEATURES Location/Qualifiers misc_feature 1106..1130 /vntifkey="21" /label=AUG1_fwd_primer misc_feature complement(1667..1688) /vntifkey="21" /label=AUG1_rev_primer CDS 3090..3665 /vntifkey="4" /label=ORF\frame\1 CDS 3671..4804 /vntifkey="4" /label=ORF\frame\3 CDS 4968..5966 /vntifkey="4" /label=ORF\frame\1 gene complement(6779..7639) /gene="Ampicillin" /vntifkey="60" CDS complement(6779..7639) /vntifkey="4" /label=ORF\frame\2 promoter complement(7681..7709) /vntifkey="30" /label=AmpR_promoter sig_peptide 1197..1463 /vntifkey="94" /label=Alpha\factor\secretion\signal BASE COUNT 2362 a 1741 c 1569 g 2376 t ORIGIN 1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag 61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt 121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac 181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca 241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac 301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa 361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc 421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa 481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg 541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc 601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa 661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct 721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct 781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg 841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa 901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat 961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct 1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt 1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac 1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga 1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag 1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact 1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg 1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc 1441 tcgagaaaag agaggctgaa gctgcaggaa ttcacgtggc ccagccggcc gtggatccac 1501 gcgtcgtcga cccgcggcgg ccgccagctt actagtaggt aagcctatcc ctaaccctct 1561 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgat ctagtataca 1621 attctagggc tgcctgtttg gatattttta taatttttga gagtttgcca actaatgttt 1681 ttctcttcta tgatatttat catgtagttg gatatctttt tttctttgaa aagtttttat 1741 ttagttcttt ctctcttttt tataatgttt tcagtttctt tcctttatat tgaaaatacg 1801 atgttctttg tctttctttt ttaaatcctc aattataatg tgtaccgttt gatcaacttg 1861 taaaattgtt tgtagtcctg taatcttaat tgacgattgg ttgatagttt accccagtat 1921 attgtgcggc tgagatatta cccgattcgg aagattgatt ttttcaacag acataaaccc 1981 ataggagatt tagtacagcg acatatgcta attgcctgat aatgctcact aacattttct 2041 ccttttaatc tggtatatgc atacaggtat gttgatatgg ccttgttgat caaagctacg 2101 aaagtgtaga ctatttccca aacagccccc aaccaccacc tcacacgcgc acactgcgaa 2161 gtgaaagcgc tcccttattt tcttctccgc tggttcaaaa caaaaaagaa tctaatctgt 2221 agacacacgc cccgtaaaac ccaatcttca tcatcgtaat tttcagtgac ggacgatcga 2281 tcaaccatat agtttgttag tgactgagct tcagtagttg acctcttgaa gaaataacgt 2341 cttgaacaac atactttata cctaccaaca ttcgccatca acaacagtgt gacactgaca 2401 taactttaca tccattcatc gacaaaaaaa tgtctctagt tcggattcat gcttatgggt 2461 tagctatttc gcccaatgtg tccatctgac attactattt tgcattttaa tttaattaga 2521 acttgactag cgcactacca gcatatcatc tcatttccgt aaataccaaa tgtattatat 2581 attgaaagct tttgaccagg ttattataaa agaaacttca tgctcgaaaa agatcatttc 2641 gaaaagttgc ctagtttcat gaaattttaa agcagtttat ataaatttta ccttttgatg 2701 cggaattgac tttttcttga ataatacata acttttctta aaagaatcaa agacagataa 2761 aatttaagag atattaaata ttagtgagaa gccgagaatt ttgtaacacc aacataacac 2821 tgacatcttt aacaactttt aattatgata catttcttac gtcatgattg attattacag 2881 ctatgctgac aaatgactct tgttgcatgg ctacgaaccg ggtaatacta agtgattgac 2941 tcttgctgac cttttattaa gaactaaatg gacaatatta tggagcattt catgtataaa 3001 ttggtgcgta aaatcgttgg atctctcttc taagtacatc ctactataac aatcaagaaa 3061 aacaagaaaa tcggacaaaa caatcaagta tggattctag aacagttggt atattaggag 3121 ggggacaatt gggacgtatg attgttgagg cagcaaacag gctcaacatt aagacggtaa 3181 tactagatgc tgaaaattct cctgccaaac aaataagcaa ctccaatgac cacgttaatg 3241 gctccttttc caatcctctt gatatcgaaa aactagctga aaaatgtgat gtgctaacga 3301 ttgagattga gcatgttgat gttcctacac taaagaatct tcaagtaaaa catcccaaat 3361 taaaaattta cccttctcca gaaacaatca gattgataca agacaaatat attcaaaaag 3421 agcatttaat caaaaatggt atagcagtta cccaaagtgt tcctgtggaa caagccagtg 3481 agacgtccct attgaatgtt ggaagagatt tgggttttcc attcgtcttg aagtcgagga 3541 ctttggcata cgatggaaga ggtaacttcg ttgtaaagaa taaggaaatg attccggaag 3601 ctttggaagt actgaaggat cgtcctttgt cgccgaaaaa tgggcaccat ttactaaaga 3661 attagcagtc atgattgtga gatctgttaa cggtttagtg ttttcttacc caattgtaga 3721 gactatccac aaggacaata tttgtgactt atgttatgcg cctgctagag ttccggactc 3781 cgttcaactt aaggcgaagt tgttggcaga aaatgcaatc aaatcttttc ccggttgtgg 3841 tatatttggt gtggaaatgt tctatttaga aacaggggaa ttgcttatta acgaaattgc 3901 cccaaggcct cacaactctg gacattatac cattgatgct tgcgtcactt ctcaatttga 3961 agctcatttg agatcaatat tggatttgcc aatgccaaag aatttcacat ctttctccac 4021 cattacaacg aacgccatta tgctaaatgt tcttggagac aaacatacaa aagataaaga 4081 gctagaaact tgcgaaagag cattggcgac tccaggttcc tcagtgtact tatatggaaa 4141 agagtctaga cctaacagaa aagtaggtca cataaatatt attgcctcca gtatggcgga 4201 atgtgaacaa aggctgaact acattacagg tagaactgat attccaatca aaatctctgt 4261 cgctcaaaag ttggacttgg aagcaatggt caaaccattg gttggaatca tcatgggatc 4321 agactctgac ttgccggtaa tgtctgccgc atgtgcggtt ttaaaagatt ttggcgttcc 4381 atttgaagtg acaatagtct ctgctcatag aactccacat aggatgtcag catatgctat 4441 ttccgcaagc aagcgtggaa ttaaaacaat tatcgctgga gctggtgggg ctgctcactt 4501 gccaggtatg gtggctgcaa tgacaccact tcctgtcatc ggtgtgcccg taaaaggttc 4561 ttgtctagat ggagtagatt ctttacattc aattgtgcaa atgcctagag gtgttccagt 4621 agctaccgtc gctattaata atagtacgaa cgctgcgctg ttggctgtca gactgcttgg 4681 cgcttatgat tcaagttata caacgaaaat ggaacagttt ttattaaagc aagaagaaga 4741 agttcttgtc aaagcacaaa agttagaaac tgtcggttac gaagcttatc tagaaaacaa 4801 gtaatatata agtttattga tatacttgta cagcaaataa ttataaaatg atatacctat 4861 tttttaggct ttgttatgat tacatcaaat gtggacttca tacatagaaa tcaacgctta 4921 caggtgtcct tttttaagaa tttcatacat aagatctact agaatcaatg ttaaacctgt 4981 tatcattaga acagccatct ccagaaggtt ctatccaagt gaaaactttg gtcttcaagc 5041 caaagactgc taaaacacaa accccagtcc caattattgt tgttgctgaa acttctactt 5101 ctactccggg tggtgttata gccaaagctt cgggcactaa agagccaaga ttagcaaacg 5161 atgaattgac caaccaattc tttggttctc ctgctaaaga gttttctatt gccagcttat 5221 caaacgaaca caaagacggt aaagtcaaga tcattattga tgctgggatc aaagatgatg 5281 actcgactaa ttacgttatt tccaacactg ttgctaagtt agttttaacc ggtaagcaat 5341 tattaggttt cttatccaac tacgaaccaa ccatcgttga ttttactgct gaagctgcaa 5401 ccgctgcccc aactccagtt gttaagaaag aacctgctgc atctggtaag cttgatgatg 5461 ccaaattaat tggtgttact gctgacaagg aaaaagattt ctccaactgg tactcccaaa 5521 ttctaatgaa gggtgaaatg ttagactact acgatgtctc tggttgttac attatgagac 5581 cagcttctta cacaatttgg gaaaagattc aatcttggtt cgattcgaag attaaagaag 5641 atggtgttac taatgcttat ttcccaatgt ttgtctcatc caaggtttta gaaaaggaaa 5701 aggaccacat cgatggtttc gctcctgaag ttgcttgggt taccagagct ggtaaatctg 5761 aattagaaga accaattgct attagaccaa cttctgaaac tgttatgggt accctgcagg 5821 ggccggcctt aattaaggcg cgcccttcct cgctcactga ctcgctgcgc tcggtcgttc 5881 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 5941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 6001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 6481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 6541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 6721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 6781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6841 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 6901 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 6961 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7021 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7081 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7141 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7201 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7261 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7321 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7381 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7441 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7501 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7561 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7621 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7681 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 7741 cgcgcacatt tccccgaaaa gtgccacctg tatgcggtgt gaaataccgc acagatgcgt 7801 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta 7861 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 7921 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca 7981 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca agggcgatgg 8041 cccactac
pMETαB其他酵母表达载体：
