pMET A
pMET A
pMET A
编号 | 载体名称 |
北京华越洋生物VECT3070 | pMET A |
pMETA载体基本信息
| 出品公司: | -- |
|---|---|
| 载体名称: | pMET A, pMETA |
| 质粒类型: | 毕赤酵母蛋白表达载体（methanoloica酵母） |
| 表达水平: | 高拷贝 |
| 诱导方法: | 甲醇诱导 |
| 启动子: | P-AUG1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 7778 bp |
| 5' 测序引物及序列: | AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´ |
| 3' 测序引物及序列: | AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´ |
| 载体标签: | C-V5， C-His |
| 载体抗性: | 氨苄 |
| 筛选标记: | ADE2 |
| 备注: | 利用甲醇诱导蛋白在methanoloica酵母中表达。 |
| 产品目录号: | -- |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
pMETA载体质粒图谱和多克隆位点信息
pMETA载体序列
LOCUS pMET A 7778 bp DNA circular SYN DEFINITION pMET A ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI FEATURES Location/Qualifiers source 1..7778 /organism="pMET A" /mol_type="other DNA" misc_feature 1106..1130 /label="AUG1_fwd_primer" misc_feature complement(1397..1418) /label="AUG1_rev_primer" CDS 2820..3395 /label="ORF frame 3" CDS 3401..4534 /label="ORF frame 2" CDS 4698..5696 /label="ORF frame 3" gene complement(6509..7369) /label="Ampicillin" /gene="Ampicillin" CDS complement(6509..7369) /label="ORF frame 2" promoter complement(7411..7439) /label="AmpR_promoter" ORIGIN 1 GGCGCGCCTT AATTAAGGCC GGCCCCTGCA GGGGGCCCCG AGATGGTACA TACTTAAAAG 61 CTGCCATATT GAGGAACTTC AAAGTTTTAT CTGTTTTTAG AATTAAAAGA CGATTGTTGT 121 AACAAAACGT TGTGCCTACA TAAACTCAAA TTAATGGAAA TAGCCTGTTT TGAAAAATAC 181 ACCTTCTTAA GTACTGACAA AGTTTTGTTA AATGACTATC GAACAAGCCA TGAAATAGCA 241 CATTTCTGCC AGTCACTTTT AACACTTTCC TGCTTGCTGG TTGACTCTCC TCATACAAAC 301 ACCCAAAAGG GAAACTTTCA GTGTGGGGAC ACTTGACATC TCACATGCAC CCCAGATTAA 361 TTTCCCCAGA CGATGCGGAG ACAAGACAAA ACAACCCTTT GTCCTGCTCT TTTCTTTCTC 421 ACACCGCGTG GGTGTGTGCG CAGGCAGGCA GGCAGGCAGC GGGCTGCCTG CCATCTCTAA 481 TCGCTGCTCC TCCCCCCTGG CTTCAAATAA CAGCCTGCTG CTATCTGTGA CCAGATTGGG 541 ACACCCCCCT CCCCTCCGAA TGATCCATCA CCTTTTGTCG TACTCCGACA ATGATCCTTC 601 CCTGTCATCT TCTGGCAATC AGCTCCTTCA ATAATTAAAT CAAATAAGCA TAAATAGTAA 661 AATCGCATAC AAACGTCATG AAAAGTTTTA TCTCTATGGC CAACGGATAG TCTATCTGCT 721 TAATTCCATC CACTTTGGGA ACCGTTCTCT CTTTACCCCA GATTCTCAAA GCTAATATCT 781 GCCCCTTGTC TATTGTCCTT TCTCCGTGTA CAAGCGGAGC TTTTGCCTCC CATCCTCTTG 841 CTTTGTTTCG GTTATTTTTT TTTTCTTTTG AAACTCTTGG TCAAATCAAA TCAAACAAAA 901 CCAAACCTTC TATTCCATCA GATCAACCTT GTTCAACATT CTATAAATCG ATATAAATAT 961 AACCTTATCC CTCCCTTGTT TTTTACCAAT TAATCAATCT TCAAATTTCA AATATTTTCT 1021 ACTTGCTTTA TTACTCAGTA TTAACATTTG TTTAAACCAA CTATAACTTT TAACTGGCTT 1081 TAGAAGTTTT ATTTAACATC AGTTTCAATT TACATCTTTA TTTATTAACG AAATCTTTAC 1141 GAATTAACTC AATCAAAACT TTTACGAAAA AAAAATCTTA CTATTAAGAG CTCGAGAGGA 1201 ATTCACGTGG CCCAGCCGGC CGTGGATCCA CGCGTCGTCG ACCCGCGGCG GCCGCCAGCT 1261 TCCTAGGGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGC GTACCGGTCA 1321 TCATCACCAT CACCATTGAA CTAGTATACA ATTCTAGGGC TGCCTGTTTG GATATTTTTA 1381 TAATTTTTGA GAGTTTGCCA ACTAATGTTT TTCTCTTCTA TGATATTTAT CATGTAGTTG 1441 GATATCTTTT TTTCTTTGAA AAGTTTTTAT TTAGTTCTTT CTCTCTTTTT TATAATGTTT 1501 TCAGTTTCTT TCCTTTATAT TGAAAATACG ATGTTCTTTG TCTTTCTTTT TTAAATCCTC 1561 AATTATAATG TGTACCGTTT GATCAACTTG TAAAATTGTT TGTAGTCCTG TAATCTTAAT 1621 TGACGATTGG TTGATAGTTT ACCCCAGTAT ATTGTGCGGC TGAGATATTA CCCGATTCGG 1681 AAGATTGATT TTTTCAACAG ACATAAACCC ATAGGAGATT TAGTACAGCG ACATATGCTA 1741 ATTGCCTGAT AATGCTCACT AACATTTTCT CCTTTTAATC TGGTATATGC ATACAGGTAT 1801 GTTGATATGG CCTTGTTGAT CAAAGCTACG AAAGTGTAGA CTATTTCCCA AACAGCCCCC 1861 AACCACCACC TCACACGCGC ACACTGCGAA GTGAAAGCGC TCCCTTATTT TCTTCTCCGC 1921 TGGTTCAAAA CAAAAAAGAA TCTAATCTGT AGACACACGC CCCGTAAAAC CCAATCTTCA 1981 TCATCGTAAT TTTCAGTGAC GGACGATCGA TCAACCATAT AGTTTGTTAG TGACTGAGCT 2041 TCAGTAGTTG ACCTCTTGAA GAAATAACGT CTTGAACAAC ATACTTTATA CCTACCAACA 2101 TTCGCCATCA ACAACAGTGT GACACTGACA TAACTTTACA TCCATTCATC GACAAAAAAA 2161 TGTCTCTAGT TCGGATTCAT GCTTATGGGT TAGCTATTTC GCCCAATGTG TCCATCTGAC 2221 ATTACTATTT TGCATTTTAA TTTAATTAGA ACTTGACTAG CGCACTACCA GCATATCATC 2281 TCATTTCCGT AAATACCAAA TGTATTATAT ATTGAAAGCT TTTGACCAGG TTATTATAAA 2341 AGAAACTTCA TGCTCGAAAA AGATCATTTC GAAAAGTTGC CTAGTTTCAT GAAATTTTAA 2401 AGCAGTTTAT ATAAATTTTA CCTTTTGATG CGGAATTGAC TTTTTCTTGA ATAATACATA 2461 ACTTTTCTTA AAAGAATCAA AGACAGATAA AATTTAAGAG ATATTAAATA TTAGTGAGAA 2521 GCCGAGAATT TTGTAACACC AACATAACAC TGACATCTTT AACAACTTTT AATTATGATA 2581 CATTTCTTAC GTCATGATTG ATTATTACAG CTATGCTGAC AAATGACTCT TGTTGCATGG 2641 CTACGAACCG GGTAATACTA AGTGATTGAC TCTTGCTGAC CTTTTATTAA GAACTAAATG 2701 GACAATATTA TGGAGCATTT CATGTATAAA TTGGTGCGTA AAATCGTTGG ATCTCTCTTC 2761 TAAGTACATC CTACTATAAC AATCAAGAAA AACAAGAAAA TCGGACAAAA CAATCAAGTA 2821 TGGATTCTAG AACAGTTGGT ATATTAGGAG GGGGACAATT GGGACGTATG ATTGTTGAGG 2881 CAGCAAACAG GCTCAACATT AAGACGGTAA TACTAGATGC TGAAAATTCT CCTGCCAAAC 2941 AAATAAGCAA CTCCAATGAC CACGTTAATG GCTCCTTTTC CAATCCTCTT GATATCGAAA 3001 AACTAGCTGA AAAATGTGAT GTGCTAACGA TTGAGATTGA GCATGTTGAT GTTCCTACAC 3061 TAAAGAATCT TCAAGTAAAA CATCCCAAAT TAAAAATTTA CCCTTCTCCA GAAACAATCA 3121 GATTGATACA AGACAAATAT ATTCAAAAAG AGCATTTAAT CAAAAATGGT ATAGCAGTTA 3181 CCCAAAGTGT TCCTGTGGAA CAAGCCAGTG AGACGTCCCT ATTGAATGTT GGAAGAGATT 3241 TGGGTTTTCC ATTCGTCTTG AAGTCGAGGA CTTTGGCATA CGATGGAAGA GGTAACTTCG 3301 TTGTAAAGAA TAAGGAAATG ATTCCGGAAG CTTTGGAAGT ACTGAAGGAT CGTCCTTTGT 3361 CGCCGAAAAA TGGGCACCAT TTACTAAAGA ATTAGCAGTC ATGATTGTGA GATCTGTTAA 3421 CGGTTTAGTG TTTTCTTACC CAATTGTAGA GACTATCCAC AAGGACAATA TTTGTGACTT 3481 ATGTTATGCG CCTGCTAGAG TTCCGGACTC CGTTCAACTT AAGGCGAAGT TGTTGGCAGA 3541 AAATGCAATC AAATCTTTTC CCGGTTGTGG TATATTTGGT GTGGAAATGT TCTATTTAGA 3601 AACAGGGGAA TTGCTTATTA ACGAAATTGC CCCAAGGCCT CACAACTCTG GACATTATAC 3661 CATTGATGCT TGCGTCACTT CTCAATTTGA AGCTCATTTG AGATCAATAT TGGATTTGCC 3721 AATGCCAAAG AATTTCACAT CTTTCTCCAC CATTACAACG AACGCCATTA TGCTAAATGT 3781 TCTTGGAGAC AAACATACAA AAGATAAAGA GCTAGAAACT TGCGAAAGAG CATTGGCGAC 3841 TCCAGGTTCC TCAGTGTACT TATATGGAAA AGAGTCTAGA CCTAACAGAA AAGTAGGTCA 3901 CATAAATATT ATTGCCTCCA GTATGGCGGA ATGTGAACAA AGGCTGAACT ACATTACAGG 3961 TAGAACTGAT ATTCCAATCA AAATCTCTGT CGCTCAAAAG TTGGACTTGG AAGCAATGGT 4021 CAAACCATTG GTTGGAATCA TCATGGGATC AGACTCTGAC TTGCCGGTAA TGTCTGCCGC 4081 ATGTGCGGTT TTAAAAGATT TTGGCGTTCC ATTTGAAGTG ACAATAGTCT CTGCTCATAG 4141 AACTCCACAT AGGATGTCAG CATATGCTAT TTCCGCAAGC AAGCGTGGAA TTAAAACAAT 4201 TATCGCTGGA GCTGGTGGGG CTGCTCACTT GCCAGGTATG GTGGCTGCAA TGACACCACT 4261 TCCTGTCATC GGTGTGCCCG TAAAAGGTTC TTGTCTAGAT GGAGTAGATT CTTTACATTC 4321 AATTGTGCAA ATGCCTAGAG GTGTTCCAGT AGCTACCGTC GCTATTAATA ATAGTACGAA 4381 CGCTGCGCTG TTGGCTGTCA GACTGCTTGG CGCTTATGAT TCAAGTTATA CAACGAAAAT 4441 GGAACAGTTT TTATTAAAGC AAGAAGAAGA AGTTCTTGTC AAAGCACAAA AGTTAGAAAC 4501 TGTCGGTTAC GAAGCTTATC TAGAAAACAA GTAATATATA AGTTTATTGA TATACTTGTA 4561 CAGCAAATAA TTATAAAATG ATATACCTAT TTTTTAGGCT TTGTTATGAT TACATCAAAT 4621 GTGGACTTCA TACATAGAAA TCAACGCTTA CAGGTGTCCT TTTTTAAGAA TTTCATACAT 4681 AAGATCTACT AGAATCAATG TTAAACCTGT TATCATTAGA ACAGCCATCT CCAGAAGGTT 4741 CTATCCAAGT GAAAACTTTG GTCTTCAAGC CAAAGACTGC TAAAACACAA ACCCCAGTCC 4801 CAATTATTGT TGTTGCTGAA ACTTCTACTT CTACTCCGGG TGGTGTTATA GCCAAAGCTT 4861 CGGGCACTAA AGAGCCAAGA TTAGCAAACG ATGAATTGAC CAACCAATTC TTTGGTTCTC 4921 CTGCTAAAGA GTTTTCTATT GCCAGCTTAT CAAACGAACA CAAAGACGGT AAAGTCAAGA 4981 TCATTATTGA TGCTGGGATC AAAGATGATG ACTCGACTAA TTACGTTATT TCCAACACTG 5041 TTGCTAAGTT AGTTTTAACC GGTAAGCAAT TATTAGGTTT CTTATCCAAC TACGAACCAA 5101 CCATCGTTGA TTTTACTGCT GAAGCTGCAA CCGCTGCCCC AACTCCAGTT GTTAAGAAAG 5161 AACCTGCTGC ATCTGGTAAG CTTGATGATG CCAAATTAAT TGGTGTTACT GCTGACAAGG 5221 AAAAAGATTT CTCCAACTGG TACTCCCAAA TTCTAATGAA GGGTGAAATG TTAGACTACT 5281 ACGATGTCTC TGGTTGTTAC ATTATGAGAC CAGCTTCTTA CACAATTTGG GAAAAGATTC 5341 AATCTTGGTT CGATTCGAAG ATTAAAGAAG ATGGTGTTAC TAATGCTTAT TTCCCAATGT 5401 TTGTCTCATC CAAGGTTTTA GAAAAGGAAA AGGACCACAT CGATGGTTTC GCTCCTGAAG 5461 TTGCTTGGGT TACCAGAGCT GGTAAATCTG AATTAGAAGA ACCAATTGCT ATTAGACCAA 5521 CTTCTGAAAC TGTTATGGGT ACCCTGCAGG GGCCGGCCTT AATTAAGGCG CGCCCTTCCT 5581 CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA 5641 AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA 5701 AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC 5761 TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 5821 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC 5881 CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT 5941 CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT 6001 GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG 6061 AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA 6121 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT 6181 ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA 6241 GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT 6301 GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA 6361 CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 6421 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA 6481 GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT 6541 CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA 6601 CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT 6661 CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 6721 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA 6781 GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT 6841 CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA 6901 CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA 6961 GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 7021 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT 7081 GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG 7141 CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC 7201 TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT 7261 GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 7321 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT 7381 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT 7441 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG 7501 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGAAATTGTA 7561 AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC 7621 CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG 7681 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA 7741 GGGCGAAAAA CCGTCTATCA AGGGCGATGG CCCACTAC //
pMETA其他酵母表达载体：
