北京华越洋生物
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首页    载体及质粒    pESP-1
pESP-1
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pESP-1

pESP-1

pESP-1

 



编号

载体名称

北京华越洋VECT76304

pESP-1

 

pESP-1载体基本信息:

质粒类型:

粟酒裂殖酵母表达载体

启动子:

nmt1

载体标签:

N-GST,N-Flag

酶识别位点:

thrombin,EK

筛选标记:

LEU2-d

载体抗性:

Ampicillin

 

pESP-1载体图谱:

 

pESP-1载体简介:

The ESP® yeast protein expression and purification system uses the yeast Schizosaccharomyces pombe as the expression host and the glutathione S-transferase (GST) peptide as the protein purification tag. This system provides an easy alternative to protein production in E. coli. Proteins expressed in E. coli may lack proper biological function and antigenicity because of the absence of eukaryotic posttranslational modifications. S. pombe is a single-cell eukaryotic organism with properties similar to higher eukaryotic organisms. These properties, such as chromosome structure and function, cell-cycle control, RNA splicing and codon usage, make S. pombe ideal for the production of eukaryotic proteins. Also, eukaryotic proteins expressed in S. pombe are more likely to be folded properly, which improves the specific activity and can eliminate protein insolubility problems found in E. coli expression systems.

Location of Features

ARS1 origin 65–1270
ampicillin resistance (bla) ORF 1401–2258
pUC origin 2409–3076
yeast LEU2-d (promoter mutant) ORF 5464–6555
S. pombe nmt1 promoter 7001–8165
GST affinity tag 8168–8818
thrombin target 8870–8881
FLAG tag 8828–8845
EK target 8846–8869
multiple cloning site 8855–8869
S. pombe nmt1 terminator 8885–9879
f1 origin 10091–10397

 

pESP-1载体相关的酵母表达载体:

pKLAC1YIplac211
pHiSi-1YEplac181
pHISiYCplac22
pGBKlampYX212-VHB
pGBK-53pYES2-flag
pGAD424pYES2/NT C
pGAD53mpYES2/NT B
pFLD-cat Z+pYES2/NT A
pFLD-cat A+pYES2/CT/α-Factor
pFLD1 Z+pYADE4
pESP-3pUG35
pESP-2pP-αSUMO3
pESP-1pPICZalpha GB
pDR196pPICZalpha GA
pDNR1pPICZalpha FC
pBLADE-SXpPICZalpha E
pACT2 ADpMETα C
p426 GALpMETα B
P416 GPDpMETα A
pPIC6αApYD1
pRS406p53his
pRS416pCL1
pSEP1pSos
pHisSi-1pGBKT7-53
pMyrpFLDα
pGBKT7-LampADH2
pDisplaypYIP5
pPinkα-HCpDR195
pPIC3.5KpYES2
pAUR123pPIC ZαD
pPICZ CYcplac33
pHIC-PIpYIP211
pRS426galpYES2-kan
p416GFDpPICZα B
pYCP211pESC-Leu
pRS426pYES2-NTC
pESC-TRPpMETαB
pYES3/CTpMET A
pMETαCpHIL-S1
pMET BpPICZα C
pHIL-D2pPIC3.5
pGAPZα ApUG66
pAO815pDEST32
pGAPZ CpYC2/NT A
pYC2/NT CpYES-DEST52
pYES2/CTpPIC6αC
pPIC6 ApRS403
pGAG424pSos-MAFB
pGADT7-TpBridge
pHis2pACT2-AD
pGBKT7pFLD/CAT
pFLDpYES2-EGFP
Ycp22lac-EGFPpPIC9k-His
SUMO proteasepPICZ A
pGADT7LA503
BFD15pPIC ZαFC
pPIC ZαGBpYEPlac112
pYEPlac195pYRP7
pYX212pFA6a-FGP(S65T)-kanMX6
pRS41HpESC-URA
pRS316pYES6/CT
pESC-HispYES2-NTB
pYES2-NTApMET C
pMETαApPink-HC
pPink-LCpGAPZ B
pGAPZα CpPICZα A
pPICZ BpYC2/CT
pPIC9pRS405
pGAPZ ApRS414
pAD-GAL4-2.1pSEP2
pYC2/NT Bp53blue
pPIC6 CpRS415
pPIC6αBpSEP3
pRS413pBD-GAL4-Cam