北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pBLADE-SX
pBLADE-SX

pBLADE-SX

pBLADE-SX

pBLADE-SX



 

编号

载体名称

北京华越洋VECT76326

pBLADE-SX

 

pBLADE-SX载体基本信息:

质粒类型:

酵母表达载体

载体大小:

5064 bp

载体抗性:

Ampicillin (氨苄青霉素)

筛选标记:

ADE1

 

pBLADE-SX载体图谱:

pBLADE-SX载体序列:

 

AGCTCCAAGGCAACAAATTGACTACTCAGACCGACATTCATTCGTTATTGATTTTAAATCAACGATAAAC
GGAATGGTTACTTGAATGATTTCACTTTATGATCATTGTTTACTAATTACCTAAATAGGATTTTATATGG
AATTGGAAGAATAAGGGAAATTTCAGATGTCTGAAAAAGGCGAGGAGGGTACTAATCATTCAAGCCCATT
TCTTGCCAGTAATTGCTTCATAAGCTTCAATATACTTTTCTTTACTCTTGATAGCAATTTCTGCATCCAT
GGCTACGCCCTCTTTGCCATTCAATCCGTTGGCCGTCAACCAATCTCTGAGAAACTGCTTATCGTAACTC
TCTTGCGATTTACCCACTTGGTAAGTCTTTTGATTCCAAAATCTAGAAGAATCTGGAGTTAAAACTTCAT
CTACTAGTACCAATTCATTGTTTTCGTCCAGTCCAAATTCGAATTTCGTATCAGCAATAATGATCCCCTT
CAAAAGGGCGAAGTTTTTTGCAGCAGAATACAACTCGACCGCCTTGACAGCGACCTTCTCACAAATGTCT
TTACCTACAATCTCAGCAGCTTGTTCAATAGAGATGTTTTCATCGTGTTCACCCTGTTCAGCTTTCGTTG
AAGGTGTGAAAATCGGAGTTGGAAAGGCGTCGCTCTCTTGAAGGTTCTCGTTTTCAACCTTGACTCCATG
GACAGTTTTTGAGTTCTTGTACTCTTTCCATGCACTTCCAGTGATGTAACCTCTGACAATGGCTTCCAAA
GGTATCAGTCTGTGCTTTTTTACTATCAAGGATCGTCCCTCTAATTGAGATTTGTATTTTTCTTCAGACA
GTTTTGATGGTAGTAAAGCAAAGACTTCCTTGTCATTAGAAGCAACCAAATGATTCTTTATGTAGGGTGC
CAAAAAATCAAACCAGAAAACTGAGAGCTGAGTCAAAATCTTTCCCTTATCAGGAATACCGTTTGTCATA
ATCACATCGTAAGCGGAGATACGGTCAGTTGCGACGAACAGCAAGTTGTTCTCATCGACTGCATAAATGT
CTCTAACCTTTCCTTTGGCGATTAAAGGTAGGATTCCGTCCAGATCAGTGTTCACAATGGACATACTTGG
AAGGATACAGCAAAGTGTGTTGGAAGCGATGACACATGGAAAGGAATTTTTCGAGTTTCCTAGAGTAGTA
TATTGGGGCGGTGAAAGTTCAGATGTTTAATGCTTAATACTCTTATACTCTTCAAAGCGCCCAAGTGTTT
CTGCCAACCTGACTTTTTTCTGAATAATGAATCGTTCAAGTGGAGTATTTAAACCATGATTAAGTTACGT
GATTTGGCACTGGATAAGGTCGAAAAATATCCGTATTCATAAACGATTATTGGTAAAAGTTACAAAATAC
CACTAATTACGGAGAAGCTTAGTAACAGTTATCATCTCTTGGTCGATTAACGCTTACAATTTCCATTCGC
CATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGGCGGATCC
GCACAAACGAAGGTCTCACTTAATCTTCTGTACTCTGAAGAGGAGTGGGAAATACCAAGAAAAACATCAA
ACTCGAATGATTTTCCCAAACCCCTACCACAAGATATTCATCAGCTGCGAGATAGGCTGATCAGGAGCAA
GCTCGTACGAGAAGAAACAAAATGACAAAAAAAATCCTATACTATATAGGTTACAAATAAAAAAGTATCA
AAAATGAAGCCTGCATCTCTCAGGCAAATGGCATTCTGACATCCTCTTTTAGAATCTAGCAAGACCGGTC
TTCTCGTAAGTGCCCAACTTGAACTGAGGAACAGTCATGTCTAAGGCTACAAACTCAATGATGATGATGA
TGATGGTCGACGGCGCTATTCAGATCCTCTTCTGAGATGAGTTTTTGTTCTAGAAAGCTGGCGGCCGCCG
CGGCTCGAGGTACCGATCCGAGACGGCCGGCTGGGCCACGTGAATTCCTGCAGCTTCAGCCTCTCTTTTC
TCGAGAGATACCCCTTCTTCTTTAGCAGCAATGCTGGCAATAGTAGTATTTATAAACAATAACCCGTTAT
TTGTGCTGTTGGAAAATGGCAAAACAGCAACATCGAAATCCCCTTCTAAATCTGAGTAACCGATGACAGC
TTCAGCCGGAATTTGTGCCGTTTCATCTTCTGTTGTAGTGTTGACTGGAGCAGCTAATGCGGAGGATGCT
GCGAATAAAACAGCAGTAAAAATTGAAGGAAATCTCATCGTTTCGAATAATTAGTTGTTTTTTGATCTTC
TCAAGTTGTCGTTAAAAGTCGTTAAAATCAAAAGCTTGTCAATTGGAACCAGTCGCAATTATGAAAGTAA
GCTAATAATGATGATAAAAAAAAAGGTTTAAGACAGGGCAGCTTCCTTCTGTTTATATATTGCTGTCAAG
TAGGGGTTAGAACAGTTAAATTTTGATCATGAACGTTAGGCTATCAGCAGTATTCCCACCAGAATCTTGG
AAGCATACAATGTGGAGACAATGCATAATCATCCAAAAAGCGGGTGTTTCCCCATTTGCGTTTCGGCACA
GGTGCACCGGGGTTCAGAAGCGATAGAGAGACTGCGCTAAGCATTAATGAGATTATTTTTGAGCATTCGT
CAATCAATACCAAACAAGACAAACGGTATGCCGACTTTTGGAAGTTTCTTTTTGACCAACTGGCCGTTAG
CATTTCAACGAACCAAACTTAGTTCATCTTGGATGAGATCACGCTTTTGTCATATTAGGTTCCAAGACAG
CGTTTAAACTGTCAGTTTTGGGCCATTTGGGGAACATGAAACTATTTGACCCCACACTCAGAAAGCCCTC
ATCTGGAGTGATGTTCGGGTGTAATGCGGAGCTTGTTGCATTCGGAAATAAACAAACATGAACCTCGCCA
GGGGGGCCAGGATAGACAGGCTAATAAAGTCATGGTGTTAGTAGCCTAATAGAAGGAATTGGAATGAGCG
AGCTCCAATCAAGCCCAATAACTGGGCTGGTTTTTCGATGGCAAAAGTGGGTGTTGAGGAGAAGAGGAGT
GGAGGTCCTGCGTTTGCAACGGTCTGCTGCTAGTGTATCCCCTCCTGTTGCGTTTGGCACTTATGTGTGA
GAATGGACCTGTGGATGTCGGATGGCAAAAAGAGATCTCGGCTGCATTAATGAATCGGCCAACGCGCGGG
GAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGG
CTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAG
GAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTT
CCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACA
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTA
TCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGC
TGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAG
CCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAA
CTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGA
GTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAA
CGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAAT
TAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAA
TCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTA
GATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCA
CCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTT
TATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT
GCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGC
TCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCG
GTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAA
TTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGA
GAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCA
GAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTT
GAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTT
TCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAA
TACTCATACTCTTCCTTTTTCAAT

 

pBLADE-SX载体相关的酵母表达载体:

pKLAC1YIplac211
pHiSi-1YEplac181
pHISiYCplac22
pGBKlampYX212-VHB
pGBK-53pYES2-flag
pGAD424pYES2/NT C
pGAD53mpYES2/NT B
pFLD-cat Z+pYES2/NT A
pFLD-cat A+pYES2/CT/α-Factor
pFLD1 Z+pYADE4
pESP-3pUG35
pESP-2pP-αSUMO3
pESP-1pPICZalpha GB
pDR196pPICZalpha GA
pDNR1pPICZalpha FC
pBLADE-SXpPICZalpha E
pACT2 ADpMETα C
p426 GALpMETα B
P416 GPDpMETα A
pPIC6αApYD1
pRS406p53his
pRS416pCL1
pSEP1pSos
pHisSi-1pGBKT7-53
pMyrpFLDα
pGBKT7-LampADH2
pDisplaypYIP5
pPinkα-HCpDR195
pPIC3.5KpYES2
pAUR123pPIC ZαD
pPICZ CYcplac33
pHIC-PIpYIP211
pRS426galpYES2-kan
p416GFDpPICZα B
pYCP211pESC-Leu
pRS426pYES2-NTC
pESC-TRPpMETαB
pYES3/CTpMET A
pMETαCpHIL-S1
pMET BpPICZα C
pHIL-D2pPIC3.5
pGAPZα ApUG66
pAO815pDEST32
pGAPZ CpYC2/NT A
pYC2/NT CpYES-DEST52
pYES2/CTpPIC6αC
pPIC6 ApRS403
pGAG424pSos-MAFB
pGADT7-TpBridge
pHis2pACT2-AD
pGBKT7pFLD/CAT
pFLDpYES2-EGFP
Ycp22lac-EGFPpPIC9k-His
SUMO proteasepPICZ A
pGADT7LA503
BFD15pPIC ZαFC
pPIC ZαGBpYEPlac112
pYEPlac195pYRP7
pYX212pFA6a-FGP(S65T)-kanMX6
pRS41HpESC-URA
pRS316pYES6/CT
pESC-HispYES2-NTB
pYES2-NTApMET C
pMETαApPink-HC
pPink-LCpGAPZ B
pGAPZα CpPICZα A
pPICZ BpYC2/CT
pPIC9pRS405
pGAPZ ApRS414
pAD-GAL4-2.1pSEP2
pYC2/NT Bp53blue
pPIC6 CpRS415
pPIC6αBpSEP3
pRS413pBD-GAL4-Cam