北京华越洋生物
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pCMV5

pCMV5
ꄴ上一个： pCMV6-AC-GFP
ꄲ下一个： pCI

pCMV5


 

编号

载体名称

北京华越洋VECT76045

pCMV5

 

pCMV5载体基本信息：

质粒类型:

哺乳动物表达载体

克隆方法:

多克隆位点，限制性内切酶

载体大小:

4657 bp 

5' 测序引物及序列:

pCMV5 Fwd CGCAAATGGGCGGTAGGCGTG

3' 测序引物及序列:

pCMV5 Rev CCTCCACCCCATAATATTATAGAAGGACAC

载体抗性:

Ampicillin

 

pCMV5载体序列：


CCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCA
GCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGT
TGTAAAACGACGGCCAGTGCCAAGCTGATCTATACATTGAATCAATATTGGCAATTAGCCATATTAGTCA
TTGGTTATATAGCATAAATCAATATTGGCTATTGGCCATTGCATACGTTGTATCTATATCATAATATGTA
CATTTATATTGGCTCATGTCCAATATGACCGCCATGTTGACATTGATTATTGACTAGTTATTAATAGTAA
TCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCC
CGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCC
AATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAA
GTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCC
AGTACATGACCTTACGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGT
GATGCGGTTTTGGCAGTACACCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCAC
CCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACC
CCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGT
GAACCGTCAGAATTCAGATCTGGTACCACGCGTATCGATAAGCTTGCATGCCTGCAGGTCGACTCTAGAG
GATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGC
CCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTAT
GGGGTGGAGGGGGGTGGTATGGAGCAAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGGTCTAT
TGGGAACCAAGCTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAATCTCCGCCTCCTGGGTTCAAGCGA
TTCTCCTGCCTCAGCCTCCCGAGTTGTTGGGATTCCAGGCATGCATGACCAGGCTCAGCTAATTTTTGTT
TTTTTGGTAGAGACGGGGTTTCACCATATTGGCCAGGCTGGTCTCCAACTCCTAATCTCAGGTGATCTAC
CCACCTTGGCCTCCCAAATTGCTGGGATTACAGGCGTGAACCACTGCTCCCTTCCCTGTCCTTCTGATTT
TAAAATAACTATACCAGCAGGAGGACGTCCAGACACAGCATAGGCTACCTGCCATGGCCCAACCGGTGGG
ACATTTGAGTTGCTTGCTTGGCACTGTCCTCTCATGCGTTGGGTCCACTCAGTAGATGCCTGTTGAATTG
GGTACGCGGCCAGCTTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGC
AGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAAGTCCCCAGGCTCCCCAGCA
GGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCC
CGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGA
GGCCGAGGCCGCCTCGGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTT
TGCAAAAAGCTCCTCGAGGAACTGAAAAACCAGAAAGTTAATTCCCTATAGTGAGTCGTATTAAATTCGT
AATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG
AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTG
CCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCG
GTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCG
AGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAAC
ATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCT
CCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAA
AGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGAT
ACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTC
GGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTA
TCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA
ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTA
CACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGC
TCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCA
GAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTC
ACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGA
AGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGG
CACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTAC
GATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCA
GATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCT
CCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGT
TGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCC
CAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGA
TCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTAC
TGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGT
ATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAA
AAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAG
TTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGA
GCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATAC
TCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATG
TATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGT
AGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAG
CGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAA
TCGGGGCATCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGT
GATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCT
TTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATA
AGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTT
AACAAAATATTAACAAAATATTAACGTTTACAATTTC


pCMV5载体相关的哺乳动物表达载体：

SuperCos IpDsRed2-BidpNFκB-MetLuc2-Reporter
pYr-adshuttle-3pAcGFP1-N1pEF1α-IRES-DsRed-Express2
pVitro2-neo-mcspSecTag2 ApCMV-DsRed-Express2
pUB6/V5-His /LacZpGL4.27pcDNA3.1/NT-GFP-TOPO
pUB6/V5-His CpGL4.26pEF1α-IRES-ZsGreen1
pUB6/V5-His BpACTpCMV-Tag 2A
pUB6/V5-His ApBIND-Id ControlpCMV-Tag 5B
pTracer-CMV2pTRE2pAcGFP1-C In-Fusion Ready
pSV-β-GalactosidasepRevTREp3XFLAG-CMV-14
pSIpTK-hygp3XFLAG-CMV-8
pSG5pTRE3G-LucpFLAG-CMV-2
pSFV1pSwitchpcDNA3.3-TOPO
pSecTag2/Hygro ApcDNA4/His CpcDNA6.2/cLumio-DEST
pSecTag Bc-Flag pcDNA3pCMV-tdTomato
pRluc-N2pcDNA4/TO/Myc-His ApAcGFP1-Mito
pPICZalpha DpcDNA6/myc-His BpAcGFP1-N   In-Fusion Ready
pORF-lacZpcDNA6/V5-His BpDsRed-Monomer-N In-Fusion Ready
pORF-HSV1tkpcDNA6.2/nTC-Tag-DESTpcDNA4/TO/Myc-His B
pOG44pOptiVEC-TOPOpIRES2-EGFP
pNTAP-BpcDNA5/FRTpcDNA3.1/His   C
pMEP4pGL4.30pcDNA3.1/CT-GFP-TOPO
pLVX-ZsGreen-miRNA-PuropGL4.19pEF1α-IRES-AcGFP1
pLVX-IRES-Puro-3xFlagpACT-MyoDpcDNA3.2/V5/GW/D-TOPO
pLPCXpCMV-BDpcDNA4/TO/Myc-His/LacZ
pLEGFP-N1pCMV-Tet3GpcDNA4/HisMax-TOPO
pKH3pTet   on advancedp3XFLAG-CMV-13
pIRES-puro2pTRE-Tightp3xFLAG-CMV-10
phRL-TKpINDpFLAG-CMV-3
pG5lacpGene/V5-His BpcDNA4/TO/Myc-His C
pFR-lucpOPRSVIpcDNA6.2/C-YFP-DEST
pEF6/myc-His CpcDNA4/HisMax ApcDNA6.2/cTC-Tag-DEST
pEF4/V5-His ApIRESpuro3pcDNA6.2/nGeneBLAzer-DEST
pEF1/myc-His lacZpIRESneo3pCRE-MetLuc2-Reporter
pEF1/myc-His CpIRESneo2pEF1α-DsRed-Express2
pEF1/myc-His BpcDNA4/myc-His ApDsRed-Express-C1
pEF1/myc-His ApCMV-PKApEF1α-DsRed-Monomer-N1
pECFP-MitopAcGFP1-N3pDD-AmCyan1 Reporter
pECFP-ERpcDNA5/FRT/TOpCRE-DD-AmCyan1
pDP8rspBApo-CMV-PurpIRES2-DsRed-Express2
pDP5rspBApo-EF1α-purpDsRed-Express-N1
pDP4rsptdTomato-N1pcDNA6.2/nLumio-DEST
pDP3rspAcGFP1-GolgipcDNA6/myc-His A
pDP2rspAcGFP1-p53pcDNA6/V5-His A
pDP1rspAcGFP1-ActinpcDNA5/FRT/TO-TOPO
pCMV-Myc-CpBI-CMV2pcDNA6.2/V5/GW/D-TOPO
pCMV-HA-NpEF1α-tdTomatopcDNA6.2/cGeneBLAzer-DEST
pCMV-HA-CpEF1α-tdTomatopcDNA6.2/nGeneBLAzer-GW/D-TOPO
pCMV6-XL4pEBVHis ApcDNA5/TO
pCMV6-AC-GFPpGL4.10pBApo-CMV-neo
pCMV5pGL4.29pDsRed-Monomer
pCIpGL4.13pIRES
pCGNpG5 luciferasepIRES-hrGFP-1a
pcDNA6.2/EmGFP-Bsd/V5-DESTpCMV-ADpDsRed-Express2-N1
pcDNA4/V5-His ApRevTet-OffpCMV-Tag 3B
pcDNA3-mRFPpTet-OffpCRE-hrGFP
pcDNA3-CFPpTRE2-hygropDsRED2-Mito
pcDNA3.1(+)/myc-His CpVgRxRpAcGFP1-F
pcDNA3.1(+)/myc-His ApOPI3CATpAcGFP1-C1
pcDNA3.1(+)/CATpBK-RSVpAsRed2-C1
pcDNA3.1(-)/myc-His CpIRES2-DsRed2pAsRed2-N1
pcDNA3.1/Zeo(+)pCMV-MycpAcGFP1-Lam
pcDNA3.1/Zeo(-)pCMV-Tag 2CpAcGFP1-C
pcDNA3.1/V5-His CpCMV-Tag 5ApSEAP2-Basic
pcDNA3.1/Hygro(+)pCMV-Tag 3CpBI-CMV3
pcDNA3.1/Hygro(-)p3XFLAG-CMV-7pNFkB-DD-tdTomato
pcDNA3.1/GSp3XFLAG-CMV-9pcDNA3.1/His A
pBS185 CMV-CrepFLAG-CMV-4pEBVHis B
pBIND-GFPpBI-CMV4pGL4.75
pBiFC-VN155(I152L)pcDNA4/His ApGL4.20
pBiFC-CC155pcDNA4/myc-His BpCMV-SPORT6
pBiFC-CrN173pcDNA4/HisMax CpCMV-SPORT6
pBiFC-bJunVN155(I152L)pCMV-Tag 4ApBIND
pBD-p53pcDNA6/myc-His CpBD-NF-κB
pBD-NF-kBpCMV-Tag 2BpRevTet-On
pBC1pGRN145pTet-On
pAD-TRAFpCMV-MEK1pTRE3G
pAD-SV40TpCMV-Tag 3ApcDNA4/TO
pAAV-ZsGreen-miRNApCMVLacIpcDNA4/His B
pAAV-tTS-shRNApBI-CMV1pcDNA4/HisMax B
pIREShyg3pEF1α-AcGFP1-N1pcDNA4/myc-His C
pcDNA6/TRpCMV-LacZpcDNA3.1/His B
pDsRed-Express2-C1pCMV-Tag 4BpcDNA6/V5-His C
pBI-CMV5pCMV-Tag 5CpCHO1.0
pAmCyan1-C1plRES2-ZsGreen1pGL3-Promoter
pAcGFP1-Memp3XFLAG-CMV-7.1pCMV-MEKK1
pAcGFP1-C3pFLAG-CMV-5apFLAG-CMV2
pTT5pBudCE4.1pAcGFP1-C2
pSEAP2-ControlpREP4ptdTomato-C1
pIRES2-AcGFP1pBApo-CMVpCRE-DD-tdTomato
pAcGFP1-Hyg-C1

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