pQE-TriSystem-6
pQE-TriSystem-6
编号 | 载体名称 |
北京华越洋VECT90069 | pQE-TriSystem-6 |
pQE-TriSystem-6 pQE系列表达载体基本信息：
启动子: | p10 |
复制子: | ColE1 ori |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；pQE系列表达质粒 |
质粒大小: | 5302bp |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | M15 |
诱导方式: | IPTG或乳糖及其类似物 |
pQE-TriSystem-6载体质粒图谱和多克隆位点信息：
pQE-TriSystem-6载体简介
pQE-TriSystem-6载体序列：
ORIGIN
1 TCCTGCATCT TTTAATCAAA TCCCAAGATG TGTATAAACG CGCCGGTATG TACAGGAAGA
61 GGTTTATACT AAACTGTTAC ATTGCAAACG TGGTTTCGTG TGCCAAGTGT GAAAACCGAT
121 GTTTAATCAA GGCTCTGACG CATTTCTACA ACCACGACTC CAAGTGTGTG GGTGAAGTCA
181 TGCATCTTTT AATCAAATCC CAAGATGTGT ATAAACCACC AAACTGCCAA AAAATGAAAA
241 CTGTCGACAA GCTCTGTCCG TTTGCTGGCA ACTGCAAGGG TCTCAATCCT ATTTGTAATT
301 ATTGAATAAT AAAACAATTA TAAATGTCAA ATTTGTTTTT TATTAACGAT ACAAACCAAA
361 CGCAACAAGA ACATTTGTAG TATTATCTAT AATTGAAAAC GCGTAGTTAT AATCGCTGAG
421 GTAATATTTA AAATCATTTT CAAATGATTC ACAGTTAATT TGCGACAATA TAATTTTATT
481 TTCACATAAA CTAGACGCCT TGTCGTCTTC TTCTTCGTAT TCCTTCTCTT TTTCATTTTT
541 CTCTTCATAA AAATTAACAT AGTTATTATC GTATCCATAT ATGTATCTAT CGTATAGAGT
601 AAATTTTTTG TTGTCATAAA TATATATGTC TTTTTTAATG GGGTGTATAG TACCGCTGCG
661 CATAGTTTTT CTGTAATTTA CAACAGTGCT ATTTTCTGGT AGTTCTTCGG AGTGTGTTGC
721 TTTAATTATT AAATTTATAT AATCAATGAA TTTGGGATCG TCGGTTTTGT ACAATATGTT
781 GCCGGCATAG TACGCAGCTT CTTCTAGTTC AATTACACCA TTTTTTAGCA GCACCGGATT
841 AACATAACTT TCCAAAATGT TGTACGAACC GTTAAACAAA AACAGTTCAC CTCCCTTTTC
901 TATACTATTG TCTGCGAGCA GTTGTTTGTT GTTAAAAATA ACAGCCATTG TAATGAGACG
961 CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA TAGTTGCTGA TGGCCGGCCG
1021 TAATGAGACG CACAAACTAA TATCACAAAC TGGAAATGTC TATCAATATA TAGTTGCTCT
1081 AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC
1141 GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG
1201 ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA
1261 TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA
1321 AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC
1381 ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC
1441 ATGCATGGTC GAGGTGAGCC CCACGTTCTG CTTCACTCTC CCCATCTCCC CCCCCTCCCC
1501 ACCCCCAATT TTGTATTTAT TTATTTTTTA ATTATTTTGT GCAGCGATGG GGGCGGGGGG
1561 GGGGGGGGGG CGCGCGCCAG GCGGGGCGGG GCGGGGCGAG GGGCGGGGCG GGGCGAGGCG
1621 GAGAGGTGCG GCGGCAGCCA ATCAGAGCGG CGCGCTCCGA AAGTTTCCTT TTATGGCGAG
1681 GCGGCGGCGG CGGCGGCCCT ATAAAAAGCG AAGCGCGCGG CGGGCGGGAG TCGCTGCGCG
1741 CTGCCTTCGC CCCGTGCCCC GCTCCGCCGC CGCCTCGCGC CGCCCGCCCC GGCTCTGACT
1801 GACCGCGTTA CTCCCACAGG TGAGCGGGCG GGACGGCCCT TCTCCTTCGG GCTGTAATTA
1861 GCGCTTGGTT TAATGACGGC TTGTTTCTTT TCTGTGGCTG CGTGAAAGCC TTGAGGGGCT
1921 CCGGGAGGGC CCTTTGTGCG GGGGGAGCGG CTCGGGGCTG TCCGCGGGGG GACGGCTGCC
1981 TTCGGGGGGG ACGGGGCAGG GCGGGGTTCG GCTTCTGGCG TGTGACCGGC GGCTCTAGAG
2041 CCTCTGCTAA CCATGTTCAT GCCTTCTTCT TTTTCCTACA GCTCCTGGGC AACGTGCTGG
2101 TTATTGTGCT GTCTCATCAT TTTGGCAAAG AATTGGATCG GACCGAAATT AATACGACTC
2161 ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCGGAGT TAATCCGGGA CCTTTAATTC
2221 AACCCAACAC AATATATTAT AGTTAAATAA GAATTATTAT CAAATCATTT GTATATTAAT
2281 TAAAATACTA TACTGTAAAT TACATTTTAT TTACAATCAA AGGAGATATA CCATGATATG
2341 CAGGTCGATA TCCCGGGTGG ATCCGAATTC TCAGATCTCG GCGCGCCTGC AGGGTTCGAA
2401 GCTTACCTGC TATACACCAC CACCATCACC ATCATCACCA CCACTAGTGA CTCGAGAAGC
2461 GTTGAAATAG CGTACAAGTC GAGCACCACC ATCACCATCA CCATCACTAA GTGATTAACC
2521 TCAGGTGCAG GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCAATGCCC TGGCTCACAA
2581 ATACCACTGA GATCGATCTT TTTCCCTCTG CCAAAAATTA TGGGGACATC ATGAAGCCCC
2641 TTGAGCATCT GACTTCTGGC TAATAAAGGA AATTTATTTT CATTGCAATA GTGTGTTGGA
2701 ATTTTTTGTG TCTCTCACTC GGAAGGACAT ATGGGAGGGC AAATCATTTA AAACATCAGA
2761 ATGAGTATTT GGTTTAGAGT TTGGCAACAT ATGCCCATAT GTAACTAGCA TAACCCCTTG
2821 GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGC ATGCGGAGGA AATTCTCCTT
2881 GAAGTTTCCC TGGTGTTCAA AGTAAAGGAG TTTGCACCAG ACGCACCTCT GTTCACTGGT
2941 CCGGCGTATT AAAACACGAT ACATTGTTAT TAGTACATTT ATTAAGCGCT AGATTCTGTG
3001 CGTTGTTGAT TTACAGACAA TTGTTGTACG TATTTTAATA ATTCATTAAA TTTATAATCT
3061 TTAGGGTGGT ATGTTAGAGC GAAAATCAAA TGATTTTCAG CGTCTTTATA TCTGAATTTA
3121 AATATTAAAT CCTCAATAGA TTTGTAAAAT AGGTTTCGAT TAGTTTCAAA CAAGGGTTGT
3181 TTTTCCGAAC CGATGGCTGG ACTATCTAAT GGATTTTCGC TCAACGCCAC AAAACTTGCC
3241 AAATCTTGTA GCAGCAATCT AGCTTTGTCG ATATTCGTTT GTGTTTTGTT TTGTAATAAA
3301 GGTTCGACGT CGTTCAAAAT ATTATGCGCT TTTGTATTTC TTTCATCACT GTCGTTAGTG
3361 TACAATTGAC TCGACGTAAA CACGTTAAAT AGAGCTTGGA CATATTTAAC ATCGGGCGTG
3421 TTAGCTTTAT TAGGCCGATT ATCGTCGTCG TCCCAACCCT CGTCGTTAGA AGTTGCTTCC
3481 GAAGACGATT TTGCCATAGC CACACGACGC CTATTAATTG TGTCGGCTAA CACGTCCGCG
3541 ATCAAATTTG TAGTTGAGCT TTTTGGAATT GCGATCGCAT AACTTCGTAT AGCATACATT
3601 ATACGAAGTT ATAAGCTCGG AACGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA
3661 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC
3721 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
3781 TTCCATAGGC TCCGCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC
3841 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT
3901 CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG
3961 TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA
4021 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT
4081 ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA
4141 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA
4201 ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT
4261 TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT
4321 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGT
4381 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
4441 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
4501 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC
4561 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
4621 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
4681 GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
4741 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
4801 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
4861 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
4921 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
4981 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
5041 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
5101 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
5161 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
5221 CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
5281 TATTGTCTCA TGTCCGCGCG TT
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pQE-TriSystem-6其他相关pQE系列表达载体：
