pQE-TriSystem His-Strep1
pQE-TriSystem His-Strep1
编号 | 载体名称 |
北京华越洋VECT90073 | pQE-TriSystem His-Strep1 |
pQE-TriSystem His-Strep1 pQE系列表达载体基本信息：
启动子: | p10 |
复制子: | ColE1 ori |
终止子: | T7 terminator |
质粒分类: | 大肠杆菌载体；pQE系列表达质粒 |
质粒大小: | 5760bp |
原核抗性: | 氨苄青霉素Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | M15 |
诱导方式: | IPTG或乳糖及其类似物 |
pQE-TriSystem His-Strep1载体质粒图谱和多克隆位点信息：
pQE-TriSystem His-Strep1载体简介
pQE-TriSystem His-Strep1载体序列：
ORIGIN
1 CTCTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT
61 CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC
121 ATTGACGTCA ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG
181 TCAATGGGTG GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT
241 GCCAAGTACG CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA
301 GTACATGACC TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT
361 TACCATGCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC TCCCCCCCCT
421 CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG ATGGGGGCGG
481 GGGGGGGGGG GGGGCGCGCG CCAGGCGGGG CGGGGCGGGG CGAGGGGCGG GGCGGGGCGA
541 GGCGGAGAGG TGCGGCGGCA GCCAATCAGA GCGGCGCGCT CCGAAAGTTT CCTTTTATGG
601 CGAGGCGGCG GCGGCGGCGG CCCTATAAAA AGCGAAGCGC GCGGCGGGCG GGAGTCGTGC
661 GCGCTGCCTT CGCCCCGTGC CCCGCTCCGC CGCCGCCTCG CGCCGCCCGC CCCGGCTCTG
721 ACTGACCGCG TTACTCCCAC AGGTGAGCGG GCGGGACGGC CCTTCTCCTT CGGGCTGTAA
781 TTAGCGCTTG GTTTAATGAC GGCTTGTTTC TTTTCTGTGG CTGCGTGAAA GCCTTGAGGG
841 GCTCCGGGAG GGCCCTTTGT GCGGGGGGAG CGGCTCGGGG CTGTCCGCGG GGGGACGGCT
901 GCCTTCGGGG GGGACGGGGC AGGGCGGGGT TCGGCTTCTG GCGTGTGACC GGCGGCTCTA
961 GAGCCTCTGC TAACCATGTT CATGCCTTCT TCTTTTTCCT ACAGCTCCTG GGCAACGTGC
1021 TGGTTATTGT GCTGTCTCAT CATTTTGGCA AAGAATTGGA TCGGACCGGA AATCATAAAA
1081 AATTATTTGC TTTGTGAGCG GATAACAATT ATAATAGATT CAATTGAGGC CTCGACCACC
1141 GGGACCTTTA ATTCAACCCA ACACAATATA TTATAGTTAA ATAAGAATTA TTATCAAATC
1201 ATTTGTATAT TAATTAAAAT ACTATACTGT AAATTACATT TTATTTACAA TCAAAGGAGA
1261 TATACCATGG CGATATCCCG GGAGCTCGTG GATCCGAATT CTCAGATCTC GGCGCGCCTG
1321 CAGGTCGACG GTACCGGTTC GAAGCTTGCG GCCGCACAGC TGTATACACG TGCAAGCGCG
1381 TGGAGCCACC CGCAGTTCGA AAAACTCGAG CACCACCATC ACCATCACCA TCACTAAGTG
1441 ATTAACCTCA GGTGCAGGCT GCCTATCAGA AGGTGGTGGC TGGTGTGGCC AATGCCCTGG
1501 CTCACAAATA CCACTGAGAT CGATCTTTTT CCCTCTGCCA AAAATTATGG GGACATCATG
1561 AAGCCCCTTG AGCATCTGAC TTCTGGCTAA TAAAGGAAAT TTATTTTCAT TGCAATAGTG
1621 TGTTGGAATT TTTTGTGTCT CTCACTCGGA AGGACATATG GGAGGGCAAA TCATTTAAAA
1681 CATCAGAATG AGTTTTTGGT TTAGAGTTTG GCAACATATG CCCATATGTA ACTAGCATAA
1741 CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGCATG CGGAGGAAAT
1801 TCTCCTTGAA GTTTCCCTGG TGTTCAAAGT AAAGGAGTTT GCACCAGACG CACCTCTGTT
1861 CACTGGTCCG GCGTATTAAA ACACGATACA TTGTTATTAG TACATTTATT AAGCGCTAGA
1921 TTCTGTGCGT TGTTGATTTA CAGACAATTG TTGTACGTAT TTTAATAATT CATTAAATTT
1981 ATAATCTTTA GGGTGGTATG TTAGAGCGAA AATCAAATGA TTTTCAGCGT CTTTATATCT
2041 GAATTTAAAT ATTAAATCCT CAATAGATTT GTAAAATAGG TTTCGATTAG TTTCAAACAA
2101 GGGTTGTTTT TCCGAACCGA TGGCTGGACT ATCTAATGGA TTTTCGCTCA ACGCCACAAA
2161 ACTTGCCAAA TCTTGTAGCA GCAATCTAGC TTTGTCGATA TTCGTTTGTG TTTTGTTTTG
2221 TAATAAAGGT TCGACGTCGT TCAAAATATT ATGCGCTTTT GTATTTCTTT CATCACTGTC
2281 GTTAGTGTAC AATTGACTCG ACGTAAACAC GTTAAATAGA GCTTGGACAT ATTTAACATC
2341 GGGCGTGTTA GCTTTATTAG GCCGATTATC GTCGTCGTCC CAACCCTCGT CGTTAGAAGT
2401 TGCTTCCGAA GACGATTTTG CCATAGCCAC ACGACGCCTA TTAATTGTGT CGGCTAACAC
2461 GTCCGCGATC AAATTTGTAG TTGAGCTTTT TGGAATTATT TCTGATTGCG GGCGTTTTTG
2521 GGCGGGTTTC AATCTAACTG TGCCCGATTT TAATTCAGAC AACACGTTAG AAAGCGATGG
2581 TGCAGGCGGT GGTAACATTT CAGACGGCAA ATCTACTAAT GGCGGCGGTG GTGGAGCTGA
2641 TGATAAATCT ACCATCGGTG GAGGCGCAGG CGGGGCTGGC GGCGGAGGCG GAGGCGGAGG
2701 TGGTGGCGGT GATGCAGACG GCGGTTTAGG CTCAAATGTC TCTTTAGGCA ACACAGTCGG
2761 CACCTCAACT ATTGTACTGG TTTCGGGCGC CGTTTTTGGT TTGACCGGTC TGAGACGAGT
2821 GCGATTTTTT TCGTTTCTAA TAGCTTCCAA CAATTGTTGT CTGTCGTCTA AAGGTGCAGC
2881 GGGTTGAGGT TCCGTCGGCA TTGGTGGAGC GGGCGGCAAT TCAGACATCG ATGGTGGTGG
2941 TGGTGGTGGA GGCGCTGGAA TGTTAGGCAC GGGAGAAGGT GGTGGCGGCG GTGCCGCCGG
3001 TATAATTTGT TCTGGTTTAG TTTGTTCGCG CACGATTGTG GGCACCGGCG CAGGCGCCGC
3061 TGGCTGCACA ACGGAAGGTC GTCTGCTTCG AGGCAGCGCT TGGGGTGGTG GCAATTCAAT
3121 ATTATAATTG GAATACAAAT CGTAAAAATC TGCTATAAGC ATTGTAATTT CGCTATCGTT
3181 TACCGTGCCG ATATTTAACA ACCGCTCAAT GTAAGCAATT GTATTGTAAA GAGATTGTCT
3241 CAAGCTCGGA ACGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA
3301 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA
3361 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT
3421 CCGCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA
3481 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG
3541 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT
3601 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT
3661 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG
3721 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC
3781 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC
3841 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA
3901 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC
3961 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGTT ACCAATGCTT
4021 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
4081 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
4141 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
4201 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
4261 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
4321 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
4381 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
4441 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
4501 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
4561 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
4621 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
4681 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA
4741 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG
4801 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG
4861 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT
4921 GTCCGCGCGT TTCCTGCATC TTTTAATCAA ATCCCAAGAT GTGTATAAAC CACCAAACTG
4981 CCAAAAAATG AAAACTGTCG ACAAGCTCTG TCCGTTTGCT GGCAACTGCA AGGGTCTCAA
5041 TCCTATTTGT AATTATTGAA TAATAAAACA ATTATAAATG TCAAATTTGT TTTTTATTAA
5101 CGATACAAAC CAAACGCAAC AAGAACATTT GTAGTATTAT CTATAATTGA AAACGCGTAG
5161 TTATAATCGC TGAGGTAATA TTTAAAATCA TTTTCAAATG ATTCACAGTT AATTTGCGAC
5221 AATATAATTT TATTTTCACA TAAACTAGAC GCCTTGTCGT CTTCTTCTTC GTATTCCTTC
5281 TCTTTTTCAT TTTTCTCTTC ATAAAAATTA ACATAGTTAT TATCGTATCC ATATATGTAT
5341 CTATCGTATA GAGTAAATTT TTTGTTGTCA TAAATATATA TGTCTTTTTT AATGGGGTGT
5401 ATAGTACCGC TGCGCATAGT TTTTCTGTAA TTTACAACAG TGCTATTTTC TGGTAGTTCT
5461 TCGGAGTGTG TTGCTTTAAT TATTAAATTT ATATAATCAA TGAATTTGGG ATCGTCGGTT
5521 TTGTACAATA TGTTGCCGGC ATAGTACGCA GCTTCTTCTA GTTCAATTAC ACCATTTTTT
5581 AGCAGCACCG GATTAACATA ACTTTCCAAA ATGTTGTACG AACCGTTAAA CAAAAACAGT
5641 TCACCTCCCT TTTCTATACT ATTGTCTGCG AGCAGTTGTT TGTTGTTAAA AATAACAGCC
5701 ATTGTAATGA GACGCACAAA CTAATATCAC AAACTGGAAA TGTCTATCAA TATATAGTTG
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