北京华越洋生物
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首页    载体及质粒    pENTR5-UbCp
pENTR5-UbCpMap

pENTR5-UbCp

pENTR5-UbCp

pENTR5-UbCp

 



编号

载体名称

北京华越洋VECT511160

pENTR5-UbCp

 

pENTR5-UbCp  Gateway载体基本信息:

载体名称:

pENTR5-UbCp

质粒类型:

Gateway载体;入门载体

高拷贝/低拷贝:

高拷贝

启动子:

克隆方法:

限制性内切酶;多克隆位点

载体大小:

2720 bp

5' 测序引物及序列:

pENTR-F:   CTACAAACTCTTCCTGTTAGTTAG

3' 测序引物及序列:

pENTR-R:   ATGGCTCATAACACCCCTTG

载体标签:

载体抗性:

卡那霉素

真核筛选标记:

备注:


  pENTR5-UbCp
载体本身含有CCDb致死基因,能够使得普通的DH5alpha等细菌死亡,必须使用DB3.1感受态细胞进行质粒扩增;

构建Gateway系统入门克隆时需要使用ccdB敏感的 OmniMAX2感受态细胞。
   

病毒/非病毒:

非病毒

 

pENTR5-UbCp载体质粒图谱和多克隆位点信息:

pENTR5-UbCp载体简介

pENTR5-UbCp载体序列:

ORIGIN

        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA

       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAACGGAAGA

      121 GCGCCCAATA CGCAAACCGC CCTCTCCCCG CGCGTTGGCC GATTCATTAA ATGCAGCTGG

      181 CACGACAGGT TTTCCCGACT GGAAAGCGGG CAGTGGAGCG CAACGCAATT AATACCCGTA

      241 CCCGCTTACC CAGGAAAAGT TTGTAAAAAC GCAAAAAAGG CCATCCCTCC AGGATGGCCT

      301 TCTGCTTAAT TTGGATGCCT GGCAAGTTTA TGGGGGGGGT CTGCCCGCCA CCCTCCGGGC

      361 CGTTGCTTCC ACAACGTTCA AATCCGCTCC CCGCGGATTT GTCCTACTCA GGAGAGCGTT

      421 CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTTCCCG ACTGAGCCTT TCGTTTTATT

      481 TGATGCCTGG CAGTTCCCTA CTCTCGCGTT AACGCTAGCA TGGATGTTTT CCCAGTCACG

      541 ACGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCGCG TTAACGCTAC CATGGAGCTC

      601 CAAATAATGA TTTTATTTTG ACTGATAGTG ACCTGTTCGT TGCAACAAAT TGATAAGCAA

      661 TGCTTTTTTA TAATGCCAAC TTTGTATAGA AAAGTTGGGC CTCGCGCCGG GTTTTGGCGC

      721 CTCCCGCGGG CGCCCCCCTC CTCACGGCGA GCGCTGCCAC GTCAGACGAA GGGCGCAGGA

      781 GCGTCCTGAT CCTTCCGCCC GGACGCTCAG GACAGCGGCC CGCTGCTCAT AAGACTCGGC

      841 CTTAGAACCC CAGTATCAGC AGAAGGACAT TTTAGGACGG GACTTGGGTG ACTCTAGGGC

      901 ACTGGTTTTC TTTCCAGAGA GCGGAACAGG CGAGGAAAAG TAGTCCCTTC TCGGCGATTC

      961 TGCGGAGGGA TCTCCGTGGG GCGGTGAACG CCGATGATTA TATAAGGACG CGCCGGGTGT

     1021 GGCACAGCTA GTTCCGTCGC AGCCGGGATT TGGGTCGCGG TTCTTGTTTG TGGATCGCTG

     1081 TGATCGTCAC TTGGTGAGTA GCGGGCTGCT GGGCTGGCCG GGGCTTTCGT GGCCGCCGGG

     1141 CCGCTCGGTG GGACGGAAGC GTGTGGAGAG ACCGCCAAGG GCTGTAGTCT GGGTCCGCGA

     1201 GCAAGGTTGC CCTGAACTGG GGGTTGGGGG GAGCGCAGCA AAATGGCGGC TGTTCCCGAG

     1261 TCTTGAATGG AAGACGCTTG TGAGGCGGGC TGTGAGGTCG TTGAAACAAG GTGGGGGGCA

     1321 TGGTGGGCGG CAAGAACCCA AGGTCTTGAG GCCTTCGCTA ATGCGGGAAA GCTCTTATTC

     1381 GGGTGAGATG GGCTGGGGCA CCATCTGGGG ACCCTGACGT GAAGTTTGTC ACTGACTGGA

     1441 GAACTCGGTT TGTCGTCTGT TGCGGGGGCG GCAGTTATGC GGTGCCGTTG GGCAGTGCAC

     1501 CCGTACCTTT GGGAGCGCGC GCCCTCGTCG TGTCGTGACG TCACCCGTTC TGTTGGCTTA

     1561 TAATGCAGGG TGGGGCCACC TGCCGGTAGG TGTGCGGTAG GCTTTTCTCC GTCGCAGGAC

     1621 GCAGGGTTCG GGCCTAGGGT AGGCTCTCCT GAATCGACAG GCGCCGGACC TCTGGTGAGG

     1681 GGAGGGATAA GTGAGGCGTC AGTTTCTTTG GTCGGTTTTA TGTACCTATC TTCTTAAGTA

     1741 GCTGAAGCTC CGGTTTTGAA CTATGCGCTC GGGGTTGGCG AGTGTGTTTT GTGAAGTTTT

     1801 TTAGGCACCT TTTGAAATGT AATCATTTGG GTCAATATGT AATTTTCAGT GTTAGACTAG

     1861 TAAATTGTCC GCTAAATTCT GGCCGTTTTT GGCTTTTTTG TTAGACCAAG TTTGTACAAA

     1921 AAAGTTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT

     1981 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATGC AGTCACTATG AATCAACTAC

     2041 TTAGATGGTA TTAGTGACCT GTAGAATTCG AGCTCTAGAG CTGCAGGGCG GCCGCGATAT

     2101 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC

     2161 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGAA CAATAAAACT

     2221 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC

     2281 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA

     2341 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA

     2401 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG

     2461 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC

     2521 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA

     2581 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT

     2641 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA

     2701 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA

     2761 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA

     2821 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT

     2881 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT

     2941 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA

     3001 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT

     3061 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA

     3121 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC

     3181 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTACTCTG

     3241 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT

     3301 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT

     3361 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT

     3421 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG

     3481 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC

     3541 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA

     3601 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG

     3661 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA

     3721 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG

     3781 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG

     3841 CTGGCCTTTT GCTCACATGT T

//

 

pENTR5-UbCp其他相关Gateway载体:

pLKO-DEST-puro

pcDNA6.2/V5-PL-DEST

pLenti7.3-V5-DEST

pcDNA6.2/N-YFP-DEST

pLenti6-UbC-V5-DEST

pcDNA3.2/V5-DEST

pLenti4-V5-DEST

pAd/BLOCK-iT-DEST

pLenti4-BLOCK-iT     -DEST

pT-Rex-DEST30

pIB-V5-His-DEST

pLKO-DEST-EGFP

pET-DEST42

plenti6/V5-Dest

pENTR-MCS1-IRES2-EGFP

pLenti6-tdTomato-V5-DEST

pENTR-L5-pLac-Spec-L2

pLenti6/BLOCK-iT     -DEST

pENTR-GUS

pLenti6.4/R4R2/V5-DEST

pENTR-DsRed2-MCS1     miR

pLenti6.2/V5-DEST

pENTR221

pENTR-L1-pLac-lacZa-R5

pENTR3C-Dual

pENTR11-Dual

pENTR     H1 TO

pEXP4-DEST

pDONR222

pEXP3-DEST

pDONR221-P4RP3R

pENTR5'/EF1αp

pDONR     Zeo

pEXP2-DEST

pDest40

pENTR4-FLAG

pDEST24

pENTR4

pDEST22

pENTR2B

pDEST10

pEXP1-DEST

pcDNA-DEST53

pDONR-P2RP3

pcDNA6.2-N-EmGFP-DEST

pDONR221-P1P4

pAd-BLOCK-iT-DEST

pDONR221

pLKO-DEST-YFP

pDEST26

pLenti6.2/C-Lumio/V5-DEST

pDEST15

pLenti6/TR

pcDNA-DEST40

pLenti4/TO/V5-DEST

pcDNA6/BioEase-DEST

pLenti4/V5-GW/LacZ

pcDNA6.2/GW/EmGFP-miR     negative

pENTR-MCS1-IRES2-DsRed2

pcDNA6.2/C-EmGFP-DEST

pENTR-L5-LacI-L4

pAd/CMV/V5-DEST

pENTR-L1pLaclacZaL4

pT-Rex-DEST31

pENTR11-Dual

pMT-DEST48

pENTR5'/CMVp

pLKO-DEST-hygro

pENTR4-Dual

plenti-CMV-puro-DEST

pENTR1A

pLenti6-capTEV-CT-DEST

pEF-DEST51

pLenti6.3-V5-DEST

pDONR223

pENTR-R4-pLac-Spec-R3

pDONR221-P5P4

pENTR-GeneBLAzer

pDONR221-P5P2

pENTR5-UbCp

pDONR201

pENTR3C

pDEST20

pENTR     U6

pDEST17

pENTR     2B Dual

pDEST12.2

pEBNA-DEST

pDONR-P4P1R

pET301/CT-DEST

pDONR207

pET300/NT-DEST

pDEST27

pcDNA6.2/cGeneBLAzer-DEST

pDEST14

pCMV-SPORT6

pDEST8

pCMV-SPORT6

pcDNA-DEST47

pcDNA6.2/nGeneBLAzer-DEST

pcDNA6.2-V5-DEST

pcDNA6.2/C-YFP-DEST

pcDNA3.1-nV5-DEST

pcDNA6.2/cTC-Tag-DEST

pBAD-DEST49

pcDNA6.2/cLumio-DEST

pAd-PL-DEST

pcDNA6.2/nLumio-DEST

pMT/BioEase-DEST

pDEST32

pXINSECT-DEST38

pYES-DEST52

pXINSECT-DEST39