pBAD-DEST49
pBAD-DEST49
编号 | 载体名称 |
北京华越洋VECT511183 | pBAD-DEST49 |
pBAD-DEST49载体基本信息：
载体名称: | pBAD-DEST49 |
质粒类型: | 大肠杆菌表达载体；Gateway载体 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | Gateway |
启动子: | araBAD |
载体大小: | 6160 bp |
5' 测序引物及序列: | pBAD Forward |
3' 测序引物及序列: | -- |
载体标签: | V5 Epitope Tag, Thioredoxin（N-端）, His Tag （C-端） |
载体抗性: | 氨苄青霉素（Ampicillin）、氯霉素（仅空载体） |
克隆菌株: | DB3.1(空载体复制）；TOP10（表达载体复制） |
表达菌株: | BL21系列 |
备注: | 含EK (Enterokinase)切割位点，切除N-端的HP-thioredoxin标签。 |
稳定性: | 稳表达 |
组成型/诱导型: | 诱导型（阿拉伯糖) |
病毒/非病毒: | 非病毒 |
pBAD-DEST49载体质粒图谱和多克隆位点信息：
pBAD-DEST49载体简介：
The pBAD-DEST49 Gateway destination vector is designed for rapid cloning with a Gateway entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:
The araBAD promoter for tightly regulated expression in E. coli
HP-thioredoxin fusion for improved protein yield and solubility
Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax
C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
R sites for efficient recombination with any attL-flanked Gateway entry vector
pBAD-DEST49载体序列：
ORIGIN
1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT
61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA
121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG
181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG
241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG
301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGGG ATCTGATAAA
361 ATTATTCATC TGACTGATGA TTCTTTTGAT ACTGATGTAC TTAAGGCAGA TGGTGCAATC
421 CTGGTTGATT TCTGGGCACA CTGGTGCGGT CCGTGCAAAA TGATCGCTCC GATTCTGGAT
481 GAAATCGCTG ACGAATATCA GGGCAAACTG ACCGTTGCAA AACTGAACAT CGATCACAAC
541 CCGGGCACTG CGCCGAAATA TGGCATCCGT GGTATCCCGA CTCTGCTGCT GTTCAAAAAC
601 GGTGAAGTGG CGGCAACCAA AGTGGGTGCA CTGTCTAAAG GTCAGTTGAA AGAGTTCCTC
661 GACGCTAACC TGGCCGGCTC TGGATCCGGT GATGACGATG ACAAGCTGGG AATTATCACA
721 AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT
781 TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA
841 TGGCGGCCGC ATTAGGCACC CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT
901 GGATTTTGAG TTAGGATCCG GCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA
961 AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG
1021 CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT
1081 TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG
1141 CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA
1201 TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT
1261 CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG
1321 TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT
1381 TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG
1441 ACAACTTCTT CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC
1501 TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTCTGTGA TGGCTTCCAT GTCGGCAGAA
1561 TGCTTAATGA ATTACAACAG TACTGCGATG AGTGGCAGGG CGGGGCGTAA ACGCGTGGAT
1621 CCGGCTTACT AAAAGCCAGA TAACAGTATG CGTATTTGCG CGCTGATTTT TGCGGTATAA
1681 GAATATATAC TGATATGTAT ACCCGAAGTA TGTCAAAAAG AGGTGTGCTA TGAAGCAGCG
1741 TATTACAGTG ACAGTTGACA GCGACAGCTA TCAGTTGCTC AAGGCATATA TGATGTCAAT
1801 ATCTCCGGTC TGGTAAGCAC AACCATGCAG AATGAAGCCC GTCGTCTGCG TGCCGAACGC
1861 TGGAAAGCGG AAAATCAGGA AGGGATGGCT GAGGTCGCCC GGTTTATTGA AATGAACGGC
1921 TCTTTTGCTG ACGAGAACAG GGACTGGTGA AATGCAGTTT AAGGTTTACA CCTATAAAAG
1981 AGAGAGCCGT TATCGTCTGT TTGTGGATGT ACAGAGTGAT ATTATTGACA CGCCCGGGCG
2041 ACGGATGGTG ATCCCCCTGG CCAGTGCACG TCTGCTGTCA GATAAAGTCT CCCGTGAACT
2101 TTACCCGGTG GTGCATATCG GGGATGAAAG CTGGCGCATG ATGACCACCG ATATGGCCAG
2161 TGTGCCGGTC TCCGTTATCG GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT
2221 CAAAAACGCC ATTAACCTGA TGTTCTGGGG AATATAAATG TCAGGCTCCG TTATACACAG
2281 CCAGTCTGCA GGTCGACCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT
2341 GTTTTTTATG CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT
2401 CAGCTTTCTT GTACAAAGTG GTGATCAAGC TTGAAGGTAA GCCTATCCCT AACCCTCTCC
2461 TCGGTCTCGA TTCTACGCGT ACCGGTCATC ATCACCATCA CCATTGAGTT TAAACGGTCT
2521 CCAGCTTGGC TGTTTTGGCG GATGAGAGAA GATTTTCAGC CTGATACAGA TTAAATCAGA
2581 ACGCAGAAGC GGTCTGATAA AACAGAATTT GCCTGGCGGC AGTAGCGCGG TGGTCCCACC
2641 TGACCCCATG CCGAACTCAG AAGTGAAACG CCGTAGCGCC GATGGTAGTG TGGGGTCTCC
2701 CCATGCGAGA GTAGGGAACT GCCAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT
2761 GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC
2821 CGGGAGCGGA TTTGAACGTT GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA GGACGCCCGC
2881 CATAAACTGC CAGGCATCAA ATTAAGCAGA AGGCCATCCT GACGGATGGC CTTTTTGCGT
2941 TTCTACAAAC TCTTTTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG
3001 ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA
3061 TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC
3121 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT
3181 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC
3241 AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG
3301 GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC
3361 AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT
3421 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA
3481 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC
3541 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC
3601 AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT
3661 AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC
3721 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC
3781 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA
3841 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA
3901 TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT
3961 TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA
4021 ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG
4081 AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC
4141 GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG
4201 CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA
4261 GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC
4321 CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC
4381 GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA
4441 CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG
4501 AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT
4561 TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA
4621 GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC
4681 GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT
4741 ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG
4801 CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG
4861 GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATGGTGCA CTCTCAGTAC
4921 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG
4981 GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG
5041 CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG
5101 TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCAGATCA ATTCGCGCGC GAAGGCGAAG
5161 CGGCATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC
5221 TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA
5281 TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA
5341 AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA
5401 GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG
5461 CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG
5521 CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG
5581 TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT
5641 TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC
5701 CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC
5761 GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA
5821 TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA
5881 TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA
5941 ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA
6001 TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC
6061 GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT
6121 TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG
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pBAD-DEST49其他相关Gateway载体：
