PX551
PX551
编号 | 载体名称 |
北京华越洋VECT75249 | PX551 |
PX551 载体基本信息：
载体名称: | PX551 或 pAAV-pMecp2-SpCas9-spA (AAV-SpCas9) |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；哺乳动物载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | pMecp2 |
载体大小: | 7400bp |
5' 测序引物及序列: | AATGGGGTCCGCCTCTTTTCC |
3' 测序引物及序列: | cttagctggcctccacctttctcttc |
载体标签: | 3XFLAG、SV40 NLS、NLS(N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 常规哺乳动物细胞 |
备注: | pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter. |
稳定性: | 瞬表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
PX551 载体质粒图谱和多克隆位点信息：
PX551 载体序列：
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggcctct agaaagcttA GCTGAATGGG GTCCGCCTCT
181 TTTCCCTGCC TAAACAGACA GGAACTCCTG CCAATTGAGG GCGTCACCGC TAAGGCTCCG
241 CCCCAGCCTG GGCTCCACAA CCAATGAAGG GTAATCTCGA CAAAGAGCAA GGGGTGGGGC
301 GCGGGCGCGC AGGTGCAGCA GCACACAGGC TGGTCGGGAG GGCGGGGCGC GACGTCTGCC
361 GTGCGGGGTC CCGGCATCGG TTGCGCGCac cggtgccacc atgtacccat acgatgttcc
421 agattacgct tcgccgaaga aaaagcgcaa ggtcgaagcg tccgacaaga agtacagcat
481 cggcctggac atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt
541 gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct
601 gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac
661 cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag
721 caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt
781 ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc
841 ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga
901 caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca
961 cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca
1021 gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga
1081 cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc
1141 ccagctgccc ggcgagaaga agaatggcct gttcggcaac ctgattgccc tgagcctggg
1201 cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag
1261 caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc
1321 cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag
1381 agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga
1441 gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta
1501 caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc
1561 cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga
1621 ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa
1681 cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga
1741 agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg
1801 catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag
1861 aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc
1921 cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt
1981 gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt
2041 gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc
2101 catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga
2161 ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt
2221 caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct
2281 ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga
2341 ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt
2401 gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat
2461 caacggcatc cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg
2521 cttcgccaac agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga
2581 catccagaaa gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct
2641 ggccggcagc cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct
2701 cgtgaaagtg atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa
2761 ccagaccacc cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg
2821 catcaaagag ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca
2881 gaacgagaag ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga
2941 actggacatc aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct
3001 gaaggacgac tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag
3061 cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct
3121 gaacgccaag ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg
3181 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
3241 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
3301 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
3361 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
3421 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
3481 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
3541 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
3601 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
3661 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
3721 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
3781 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
3841 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
3901 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
3961 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
4021 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
4081 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
4141 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
4201 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
4261 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
4321 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
4381 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
4441 tgacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
4501 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
4561 cgacagcccc aagaagaaga gaaaggtgga ggccagctaa gaattcAATA AAAGATCTTT
4621 ATTTTCATTA GATCTGTGTG TTGGTTTTTT GTGTgcggcc gcaggaaccc ctagtgatgg
4681 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
4741 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
4801 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
4861 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
4921 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
4981 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
5041 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
5101 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
5161 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
5221 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
5281 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
5341 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
5401 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
5461 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
5521 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
5581 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
5641 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
5701 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
5761 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
5821 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
5881 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
5941 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
6001 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
6061 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
6121 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
6181 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
6241 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
6301 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
6361 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
6421 agccggtgag cgtggaagcc gcggtatcat tgcagcactg gggccagatg gtaagccctc
6481 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
6541 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
6601 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
6661 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
6721 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
6781 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
6841 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
6901 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
6961 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
7021 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
7081 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
7141 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
7201 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
7261 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
7321 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
7381 ctggcctttt gctcacatgt
//
PX551 载体其他相关CRISPR/Cas9载体：
