PX377
PX377
编号 | 载体名称 |
北京华越洋VECT75245 | PX377 |
PX377载体基本信息：
载体名称: | PX377 或 PX377 Corynebacter diphtheriae Cas9 |
质粒类型: | CRISPR-Cas9载体；基因敲除载体；昆虫细胞载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶，多克隆位点 |
启动子: | CMV |
载体大小: | 10448bp |
5' 测序引物及序列: | CMV Forward:CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | cctagtcagacaaaatgatgcaa |
载体标签: | NLS (C ter)；HA、NLS (N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Neomycin |
克隆菌株: | Stbl3 |
宿主细胞（系）: | 昆虫细胞 |
备注: | Corynebacter diphtheriae Cas9 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 杆状病毒 |
PX377 载体质粒图谱和多克隆位点信息：
PX377 载体序列：
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccc
1021 tcgagtagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
1081 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
1141 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
1201 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
1261 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
1321 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
1381 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
1441 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
1501 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
1561 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtgg atcttaatac
1621 gactcactat aggggaattg tgagcggata acaattccgg gacctttaat tcaacccaac
1681 acaatatatt atagttaaat aagaattatt atcaaatcat ttgtatatta attaaaatac
1741 tatactgtaa attacatttt atttacaatc aacaagtttg tacaaaaaag caggctaagg
1801 agatatacca ccggtgccac catgtaccca tacgatgttc cagattacgc ttcgccgaag
1861 aaaaagcgca aggtcgaagc gtccatgaag taccatgtcg gaatcgatgt cggaaccttt
1921 tctgtggggc tggctgctat tgaagtggat gacgctggaa tgcctattaa gaccctgagt
1981 ctggtgtcac acattcatga ctcaggactg gatcctgacg agatcaagag cgctgtgacc
2041 aggctggcaa gctccggaat cgcccggaga acaaggcgcc tgtaccgacg gaagagaagg
2101 cgcctgcagc agctggataa gttcatccag aggcagggct ggccagtgat cgagctggaa
2161 gattacagcg accccctgta tccttggaag gtgcgcgccg aactggccgc ttcttatatt
2221 gctgacgaga aggaacgggg ggagaaactg agtgtggctc tgagacacat cgcaaggcat
2281 cgcggatgga ggaaccctta cgccaaggtg tctagtctgt atctgccaga tggcccctca
2341 gacgccttca aggctattag ggaggaaatc aaacgcgcta gcggccagcc tgtgccagag
2401 actgcaaccg tcgggcagat ggtgaccctg tgcgaactgg gcacactgaa gctgcgagga
2461 gagggaggag tgctgagtgc acggctgcag cagtcagatt acgcccgcga gatccaggaa
2521 atttgtcgaa tgcaggagat cggccaggaa ctgtatcgca agatcattga cgtggtgttc
2581 gcagccgagt ccccaaaggg ctctgcctca agccgggtgg ggaaagatcc tctgcagcca
2641 ggaaagaaca gagcactgaa agccagcgac gcttttcagc gataccggat tgctgcactg
2701 atcggcaatc tgagagtcag ggtggatggg gagaagagga ttctgagcgt ggaggagaag
2761 aacctggtgt tcgaccacct ggtgaatctg actccaaaga aagagcccga atgggtgacc
2821 atcgccgaaa ttctgggcat cgatcgcggg cagctgatcg gaacagctac tatgaccgac
2881 gatggagagc gagcaggagc ccgaccccct acacacgata ctaacagaag tattgtgaac
2941 agccggatcg caccactggt cgactggtgg aaaacagcta gcgcactgga gcagcacgcc
3001 atggtgaagg cactgtccaa cgccgaagtc gacgattttg attctcccga gggagcaaaa
3061 gtgcaggcat tctttgccga tctggacgat gacgtccacg ccaagctgga cagcctgcat
3121 ctgcctgtgg gacgagcagc ttactccgag gacactctgg tcagactgac ccgacggatg
3181 ctgagtgatg gggtggacct gtataccgcc cggctgcagg agttcggaat tgaacctagc
3241 tggaccccac ccacaccaag aatcggagag cctgtcggca atccagccgt cgaccgggtg
3301 ctgaaaacag tgagcagatg gctggaatcc gcaacaaaga cttggggcgc cccagagagg
3361 gtcatcattg agcacgtgcg cgaaggcttc gtcactgaga aacgcgctcg agaaatggat
3421 ggggacatga gaaggcgcgc agcccggaac gccaagctgt ttcaggagat gcaggaaaag
3481 ctgaatgtgc agggcaaacc cagtcgagcc gatctgtgga gataccagtc agtgcagaga
3541 cagaactgcc agtgtgccta ttgcgggtcc ccaattacct tttctaatag tgaaatggac
3601 cacatcgtgc ccagagcagg gcagggatcc accaacacaa gggagaatct ggtcgccgtg
3661 tgccatcgct gtaaccagtc taagggcaat acacccttcg ctatttgggc aaaaaacact
3721 tctatcgaag gggtcagtgt gaaggaggcc gtggaacgga ccagacattg ggtcactgat
3781 accggcatga gaagcactga cttcaagaag ttcaccaagg ctgtggtcga gcggtttcag
3841 agagcaacaa tggatgagga aatcgacgcc agaagcatgg aatccgtcgc ctggatggct
3901 aatgagctga ggagccgcgt ggctcagcac ttcgcatccc atggaaccac agtcagggtg
3961 taccgaggca gcctgacagc agaggctcga cgggcatctg ggatcagtgg aaagctgaaa
4021 ttctttgatg gcgtggggaa gtccaggctg gatagaaggc accatgctat tgacgctgca
4081 gtgatcgcat tcacctctga ctatgtggcc gaaacactgg ctgtccgctc aaacctgaaa
4141 cagagccagg cccaccgaca ggaggctcct cagtggagag agttcaccgg caaggatgca
4201 gagcatcgag cagcttggag agtgtggtgc cagaagatgg aaaaactgag cgccctgctg
4261 accgaggacc tgcgagatga ccgggtggtc gtgatgtcta acgtgcgact gcggctggga
4321 aatggcagtg cccacaagga aaccattggc aaactgtcaa aggtgaaact gtcctctcag
4381 ctgtcagtca gcgatatcga caaagcaagt tcagaggccc tgtggtgtgc tctgaccaga
4441 gagcccggat tcgatcctaa ggaaggcctg cccgctaacc ctgagagaca catcagggtg
4501 aatgggacac atgtctacgc cggggacaat attggactgt ttccagtgtc agcaggaagc
4561 atcgcactga ggggaggata cgcagagctg ggcagctcct tccaccatgc tcgcgtgtat
4621 aaaattactt ccggcaagaa acccgcattt gccatgctga gggtgtacac catcgatctg
4681 ctgccttatc gcaaccagga cctgtttagc gtggaactga agccacagac aatgtccatg
4741 aggcaggctg agaagaaact gcgcgacgct ctggcaactg ggaatgcaga atatctggga
4801 tggctggtcg tggatgacga gctggtcgtg gatacatcta agattgccac tgaccaggtc
4861 aaagcagtgg aggccgaact ggggactatc cgccgatggc gggtggatgg attcttttcc
4921 ccctctaaac tgagactgag gcctctgcag atgtccaagg aggggatcaa gaaagagtcc
4981 gctcccgaac tgtctaaaat cattgacaga ccaggatggc tgcccgccgt gaacaagctg
5041 ttctctgatg gaaatgtcac cgtcgtgcgg agagactctc tgggacgcgt gcgactggag
5101 agtacagccc acctgcctgt cacttggaag gtgcagtaag aattcggtac cgagctcaag
5161 cttgcggccg cctatagtgt cacctaaatc gtaacccagc tttcttgtac aaagtggtcg
5221 ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt gccactccag
5281 tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac taggtgtcct
5341 tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt gggatgacaa
5401 cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca caatcttggc
5461 tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct cccgagttgt
5521 tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg tagagacggg
5581 gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc tacccacctt
5641 ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct gtccttctag
5701 cataacccct tggggcctct aaacgggtct tgaggggttt tttgtctaga agacaatagc
5761 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
5821 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
5881 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
5941 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
6001 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
6061 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
6121 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
6181 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
6241 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
6301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
6361 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
6421 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
6481 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
6541 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
6601 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
6661 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
6721 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
6781 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
6841 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
6901 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
6961 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
7021 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
7081 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
7141 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
7201 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
7261 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
7321 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
7381 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
7441 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
7501 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
7561 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
7621 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
7681 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
7741 gtccaaactc atcaatgtat cttatcatgt ctgttcatga agccccttga gcatctgact
7801 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
7861 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
7921 tagagtttgg caacatatgc ccatatgtaa ctagcataac cccttggggc ctctaaacgg
7981 gtcttgaggg gttttttgct gaaagcatgc ggaggaaatt ctccttgaag tttccctggt
8041 gttcaaagta aaggagtttg caccagacgc acctctgttc actggtccgg cgtattaaaa
8101 cacgatacat tgttattagt acatttatta agcgctagat tctgtgcgtt gttgatttac
8161 agacaattgt tgtacgtatt ttaataattc attaaattta taatctttag ggtggtatgt
8221 tagagcgaaa atcaaatgat tttcagcgtc tttatatctg aatttaaata ttaaatcctc
8281 aatagatttg taaaataggt ttcgattagt ttcaaacaag ggttgttttt ccgaaccgat
8341 ggctggacta tctaatggat tttcgctcaa cgccacaaaa cttgccaaat cttgtagcag
8401 caatctagct ttgtcgatat tcgtttgtgt tttgttttgt aataaaggtt cgacgtcgtt
8461 caaaatatta tgcgcttttg tatttctttc atcactgtcg ttagtgtaca attgactcga
8521 cgtaaacacg ttaaatagag cttggacata tttaacatcg ggcgtgttag ctttattagg
8581 ccgattatcg tcgtcgtccc aaccctcgtc gttagaagtt gcttccgaag acgattttgc
8641 catagccaca cgacgcctat taattgtgtc ggctaacacg tccgcgatca aatttgtagt
8701 tgagcttttt ggaattgcga tcgcataact tcgtatagca tacattatac gaagttataa
8761 gctcggaacg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
8821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
8881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
8941 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
9001 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
9061 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa
9121 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
9181 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
9241 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
9301 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
9361 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
9421 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
9481 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgttacc aatgcttaat
9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgtc
10441 cgcgcgtt
//
PX377 载体其他相关CRISPR/Cas9载体：
