北京华越洋生物
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首页    载体及质粒    PX260
PX260

PX260

@PX260

 

PX260



 

编号

载体名称

北京华越洋VECT75253

PX260

 

 

PX260 载体基本信息:

载体名称:

PX260 pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

质粒类型:

CRISPR-Cas9载体;基因敲除载体;哺乳动物载体

高拷贝/低拷贝:

高拷贝

插入位点:

限制性内切酶,多克隆位点

启动子:

U6

载体大小:

10203bp

5' 测序引物及序列:

LKO.1 5'GACTATCATATGCTTACCGT

3' 测序引物及序列:

BGH ReverseTAGAAGGCACAGTCGAGG

载体标签:

3xFLAG(N ter)

载体抗性:

氨苄青霉素

筛选标记:

Puromycin

克隆菌株:

DH5alpha

宿主细胞(系):

常规哺乳动物细胞等

备注:

humanized S. pyogenes Cas9; This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

非病毒

 

PX260 载体质粒图谱和多克隆位点信息:

 

 

PX260 载体序列:

 

ORIGIN

    1 TCTCCGCCTC GCCCCGCCCC GCCCCTCGCC CCGCCCCCCC CCCCCCCCCG CCCCCATCGC

   61 TGCACAAAAT AATTAAAAAA TAAATAAATA CAAAATTGGG GGTGGGGAGG GGGGGGAGAT

  121 GGGGAGAGTG AAGCAGAACG TGGGGCTCAC CTCGACCATG GTAATAGCGA TGACTAATAC

  181 GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAC AATGCCAGGC

  241 GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG

  301 TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC

  361 TATTGGCGTT ACTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC

  421 ATTTACCGTA AGTTATGTAA CGGGTACCTC TAGAGCCATT TGTCTGCAGA ATTGGCGCAC

  481 GCGCTAGAAA AAGTTTTGGG ACCATTCAAA ACAGCATAGC TCTAAAACGT CTTCTCGAAG

  541 ACCCGTTTTG GGACCATTCA AAACAGCATA GCTCTAAAAC CCGGTGTTTC GTCCTTTCCA

  601 CAAGATATAT AAAGCCAAGA AATCGAAATA CTTTCAAGTT ACGGTAAGCA TATGATAGTC

  661 CATTTTAAAA CATAATTTTA AAACTGCAAA CTACCCAAGA AATTATTACT TTCTACGTCA

  721 CGTATTTTGT ACTAATATCT TTGTGTTTAC AGTCAAATTA ATTCCAATTA TCTCTCTAAC

  781 AGCCTTGTAT CGTATATGCA AATATGAAGG AATCATGGGA AATAGGCCCT CACATGTGAG

  841 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA

  901 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC

  961 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG

 1021 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC

 1081 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG

 1141 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC

 1201 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA

 1261 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG

 1321 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA

 1381 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG

 1441 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT

 1501 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT

 1561 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT

 1621 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA

 1681 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA

 1741 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGGCTTCCAC

 1801 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA

 1861 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG

 1921 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG

 1981 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG

 2041 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG

 2101 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC

 2161 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT

 2221 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA

 2281 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA

 2341 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA

 2401 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC

 2461 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC

 2521 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG

 2581 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC

 2641 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA

 2701 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC

 2761 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG

 2821 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG

 2881 TACTGAGAGT GCACCATAAA ATTGTAAACG TTAATATTTT GTTAAAATTC GCGTTAAATT

 2941 TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGACCGAAAT CGGCAAAATC CCTTATAAAT

 3001 CAAAAGAATA GCCCGAGATA GAGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT

 3061 TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC

 3121 TACGTGAACC ATCACCCAAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC

 3181 GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA

 3241 GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAAGGC GCTGGCAAGT GTAGCGGTCA

 3301 CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTACTATG

 3361 GTTGCTTTGA CGTATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT

 3421 CAGGCGCCGC TCCAATCCTT CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC

 3481 AAAGTATGAC AGGAAAAACT TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA

 3541 ATTTCTGCAT CCCGGGGATC TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC

 3601 GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG

 3661 CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG

 3721 GGGAGGTTGT GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC

 3781 TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG

 3841 CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC

 3901 ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG

 3961 GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG

 4021 TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC

 4081 AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG

 4141 TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG

 4201 CGCACCGTGG GCTTGTACTC GGTCATGGTA AGCTTCAGCT GCTCGAGATC TAGATGGATG

 4261 CAGGTCGAAA GGCCCGGAGA TGAGGAAGAG GAGAACAGCG CGGCAGACGT GCGCTTTTGA

 4321 AGCGTGCAGA ATGCCGGGCC TCCGGAGGAC CTTCGGGCGC CCGCCCCGCC CCTGAGCCCG

 4381 CCCCTGAGCC CGCCCCCGGA CCCACCCCTT CCCAGCCTCT GAGCCCAGAA AGCGAAGGAG

 4441 CAAAGCTGCT ATTGGCCGCT GCCCCAAAGG CCTACCCGCT TCCATTGCTC AGCGGTGCTG

 4501 TCCATCTGCA CGAGACTAGT GAGACGTGCT ACTTCCATTT GTCACGTCCT GCACGACGCG

 4561 AGCTGCGGGG CGGGGGGGAA CTTCCTGACT AGGGGAGGAG TAGAAGGTGG CGCGAAGGGG

 4621 CCACCAAAGA ACGGAGCCGG TTGGCGCCTA CCGGTGGATG TGGAATGTGT GCGAGGCCAG

 4681 AGGCCACTTG TGTAGCGCCA AGTGCCCAGC GGGGCTGCTA AAGCGCATGC TCCAGACTGC

 4741 CTTGGGAAAA GCGCCTCCCC TACCCGGTAG AATTTCGAGG TCGAGATCCT AAGCTTGGCT

 4801 GGACGTAAAC TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA

 4861 TTAAGGGTTA TTGAATATGA TCGGAATTCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG

 4921 ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT

 4981 TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG CGCCAAAAAG CACCGACTCG

 5041 GTGCCACTTT TTCAAGTTGA TAACGGACTA GCCTTATTTT AACTTGCTAT GCTGTTTTGA

 5101 ATGGTTCCAA CTAGATCTGT GGTCTCATAC AGAACTTATA AGATTCCCAA ATCCAAAGAC

 5161 ATTTCACGTT TATGGTGATT TCCCAGAACA CATAGCGACA TGCAAATATT GCAGGGCGCC

 5221 ACTCCCCTGT CCCTCACAGC CATCTTCCTG CCAGGGCGCA CGCGCGCTGG GTGTTCCCGC

 5281 CTAGTGACAC TGGGCCCGCG ATTCCTTGGA GCGGGTTGAT GACGTCAGCG TTCGAATTGC

 5341 GGCCGCTCCC CAGCATGCCT GCTATTCTCT TCCCAATCCT CCCCCTTGCT GTCCTGCCCC

 5401 ACCCCACCCC CCAGAATAGA ATGACACCTA CTCAGACAAT GCGATGCAAT TTCCTCATTT

 5461 TATTAGGAAA GGACAGTGGG AGTGGCACCT TCCAGGGTCA AGGAAGGCAC GGGGGAGGGG

 5521 CAAACAACAG ATGGCTGGCA ACTAGAAGGC ACAGTCGAGG CTGATCAGCG AGCTCTAGGA

 5581 ATTCTTACTT TTTCTTTTTT GCCTGGCCGG CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC

 5641 CTCCCAGCTG AGACAGGTCG ATCCGTGTCT CGTACAGGCC GGTGATGCTC TGGTGGATCA

 5701 GGGTGGCGTC CAGCACCTCT TTGGTGCTGG TGTACCTCTT CCGGTCGATG GTGGTGTCAA

 5761 AGTACTTGAA GGCGGCAGGG GCTCCCAGAT TGGTCAGGGT AAACAGGTGG ATGATATTCT

 5821 CGGCCTGCTC TCTGATGGGC TTATCCCGGT GCTTGTTGTA GGCGGACAGC ACTTTGTCCA

 5881 GATTAGCGTC GGCCAGGATC ACTCTCTTGG AGAACTCGCT GATCTGCTCG ATGATCTCGT

 5941 CCAGGTAGTG CTTGTGCTGT TCCACAAACA GCTGTTTCTG CTCATTATCC TCGGGGGAGC

 6001 CCTTCAGCTT CTCATAGTGG CTGGCCAGGT ACAGGAAGTT CACATATTTG GAGGGCAGGG

 6061 CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG CAGAGGCCAG CATTCTCTTC CGGCCGTTTT

 6121 CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT TGATGATCAG GTCCTTTTTC ACTTCTTTGT

 6181 AGCCCTTGGC TTCCAGAAAG TCGATGGGAT TCTTCTCGAA GCTGCTTCTT TCCATGATGG

 6241 TGATCCCCAG CAGCTCTTTC ACACTCTTCA GTTTCTTGGA CTTGCCCTTT TCCACTTTGG

 6301 CCACCACCAG CACAGAATAG GCCACGGTGG GGCTGTCGAA GCCGCCGTAC TTCTTAGGGT

 6361 CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT CGCTGTTCCT CTTGGGCAGG ATAGACTCTT

 6421 TGCTGAAGCC GCCTGTCTGC ACCTCGGTCT TTTTCACGAT ATTCACTTGG GGCATGCTCA

 6481 GCACTTTCCG CACGGTGGCA AAATCCCGGC CCTTATCCCA CACGATCTCC CCGGTTTCGC

 6541 CGTTTGTCTC GATCAGAGGC CGCTTCCGGA TCTCGCCGTT GGCCAGGGTA ATCTCGGTCT

 6601 TGAAAAAGTT CATGATGTTG CTGTAGAAGA AGTACTTGGC GGTAGCCTTG CCGATTTCCT

 6661 GCTCGCTCTT GGCGATCATC TTCCGCACGT CGTACACCTT GTAGTCGCCG TACACGAACT

 6721 CGCTTTCCAG CTTAGGGTAC TTTTTGATCA GGGCGGTTCC CACGACGGCG TTCAGGTAGG

 6781 CGTCGTGGGC GTGGTGGTAG TTGTTGATCT CGCGCACTTT GTAAAACTGG AAATCCTTCC

 6841 GGAAATCGGA CACCAGCTTG GACTTCAGGG TGATCACTTT CACTTCCCGG ATCAGCTTGT

 6901 CATTCTCGTC GTACTTAGTG TTCATCCGGG AGTCCAGGAT CTGTGCCACG TGCTTTGTGA

 6961 TCTGCCGGGT TTCCACCAGC TGTCTCTTGA TGAAGCCGGC CTTATCCAGT TCGCTCAGGC

 7021 CGCCTCTCTC GGCCTTGGTC AGATTGTCGA ACTTTCTCTG GGTAATCAGC TTGGCGTTCA

 7081 GCAGCTGCCG CCAGTAGTTC TTCATCTTCT TCACGACCTC TTCGGAGGGC ACGTTGTCGC

 7141 TCTTGCCCCG GTTCTTGTCG CTTCTGGTCA GCACCTTGTT GTCGATGGAG TCGTCCTTCA

 7201 GAAAGCTCTG AGGCACGATA TGGTCCACAT CGTAGTCGGA CAGCCGGTTG ATGTCCAGTT

 7261 CCTGGTCCAC GTACATATCC CGCCCATTCT GCAGGTAGTA CAGGTACAGC TTCTCGTTCT

 7321 GCAGCTGGGT GTTTTCCACG GGGTGTTCTT TCAGGATCTG GCTGCCCAGC TCTTTGATGC

 7381 CCTCTTCGAT CCGCTTCATT CTCTCGCGGC TGTTCTTCTG TCCCTTCTGG GTGGTCTGGT

 7441 TCTCTCTGGC CATTTCGATC ACGATGTTCT CGGGCTTGTG CCGGCCCATC ACTTTCACGA

 7501 GCTCGTCCAC CACCTTCACT GTCTGCAGGA TGCCCTTCTT AATGGCGGGG CTGCCGGCCA

 7561 GATTGGCAAT GTGCTCGTGC AGGCTATCGC CCTGGCCGGA CACCTGGGCT TTCTGGATGT

 7621 CCTCTTTAAA GGTCAGGCTG TCGTCGTGGA TCAGCTGCAT GAAGTTTCTG TTGGCGAAGC

 7681 CGTCGGACTT CAGGAAATCC AGGATTGTCT TGCCGGACTG CTTGTCCCGG ATGCCGTTGA

 7741 TCAGCTTCCG GCTCAGCCTG CCCCAGCCGG TGTATCTCCG CCGCTTCAGC TGCTTCATCA

 7801 CTTTGTCGTC GAACAGGTGG GCATAGGTTT TCAGCCGTTC CTCGATCATC TCTCTGTCCT

 7861 CAAACAGTGT CAGGGTCAGC ACGATATCTT CCAGAATGTC CTCGTTTTCC TCATTGTCCA

 7921 GGAAGTCCTT GTCCTTGATA ATTTTCAGCA GATCGTGGTA TGTGCCCAGG GAGGCGTTGA

 7981 ACCGATCTTC CACGCCGGAG ATTTCCACGG AGTCGAAGCA CTCGATTTTC TTGAAGTAGT

 8041 CCTCTTTCAG CTGCTTCACG GTCACTTTCC GGTTGGTCTT GAACAGCAGG TCCACGATGG

 8101 CCTTTTTCTG CTCGCCGCTC AGGAAGGCGG GCTTTCTCAT TCCCTCGGTC ACGTATTTCA

 8161 CTTTGGTCAG CTCGTTATAC ACGGTGAAGT ACTCGTACAG CAGGCTGTGC TTGGGCAGCA

 8221 CCTTCTCGTT GGGCAGGTTC TTATCGAAGT TGGTCATCCG CTCGATGAAG CTCTGGGCGG

 8281 AAGCGCCCTT GTCCACCACT TCCTCGAAGT TCCAGGGGGT GATGGTTTCC TCGCTCTTTC

 8341 TGGTCATCCA GGCGAATCTG CTGTTTCCCC TGGCCAGAGG GCCCACGTAG TAGGGGATGC

 8401 GGAAGGTCAG GATCTTCTCG ATCTTTTCCC GGTTGTCCTT CAGGAATGGG TAAAAATCTT

 8461 CCTGCCGCCG CAGAATGGCG TGCAGCTCTC CCAGGTGGAT CTGGTGGGGG ATGCTGCCGT

 8521 TGTCGAAGGT CCGCTGCTTC CGCAGCAGGT CCTCTCTGTT CAGCTTCACG AGCAGTTCCT

 8581 CGGTGCCGTC CATCTTTTCC AGGATGGGCT TGATGAACTT GTAGAACTCT TCCTGGCTGG

 8641 CTCCGCCGTC AATGTAGCCG GCGTAGCCGT TCTTGCTCTG GTCGAAGAAA ATCTCTTTGT

 8701 ACTTCTCAGG CAGCTGCTGC CGCACGAGAG CTTTCAGCAG GGTCAGGTCC TGGTGGTGCT

 8761 CGTCGTATCT CTTGATCATA GAGGCGCTCA GGGGGGCCTT GGTGATCTCG GTGTTCACTC

 8821 TCAGGATGTC GCTCAGCAGG ATGGCGTCGG ACAGGTTCTT GGCGGCCAGA AACAGGTCGG

 8881 CGTACTGGTC GCCGATCTGG GCCAGCAGGT TGTCCAGGTC GTCGTCGTAG GTGTCCTTGC

 8941 TCAGCTGCAG TTTGGCATCC TCGGCCAGGT CGAAGTTGCT CTTGAAGTTG GGGGTCAGGC

 9001 CCAGGCTCAG GGCAATCAGG TTTCCGAACA GGCCATTCTT CTTCTCGCCG GGCAGCTGGG

 9061 CGATCAGATT TTCCAGCCGT CTGCTCTTGC TCAGTCTGGC AGACAGGATG GCCTTGGCGT

 9121 CCACGCCGCT GGCGTTGATG GGGTTTTCCT CGAACAGCTG GTTGTAGGTC TGCACCAGCT

 9181 GGATGAACAG CTTGTCCACG TCGCTGTTGT CGGGGTTCAG GTCGCCCTCG ATCAGGAAGT

 9241 GGCCCCGGAA CTTGATCATG TGGGCCAGGG CCAGATAGAT CAGCCGCAGG TCGGCCTTGT

 9301 CGGTGCTGTC CACCAGTTTC TTTCTCAGGT GGTAGATGGT GGGGTACTTC TCGTGGTAGG

 9361 CCACCTCGTC CACGATGTTG CCGAAGATGG GGTGCCGCTC GTGCTTCTTA TCCTCTTCCA

 9421 CCAGGAAGGA CTCTTCCAGT CTGTGGAAGA AGCTGTCGTC CACCTTGGCC ATCTCGTTGC

 9481 TGAAGATCTC TTGCAGATAG CAGATCCGGT TCTTCCGTCT GGTGTATCTT CTTCTGGCGG

 9541 TTCTCTTCAG CCGGGTGGCC TCGGCTGTTT CGCCGCTGTC GAACAGCAGG GCTCCGATCA

 9601 GGTTCTTCTT GATGCTGTGC CGGTCGGTGT TGCCCAGCAC CTTGAATTTC TTGCTGGGCA

 9661 CCTTGTACTC GTCGGTGATC ACGGCCCAGC CCACAGAGTT GGTGCCGATG TCCAGGCCGA

 9721 TGCTGTACTT CTTGTCGGCT GCTGGGACTC CGTGGATACC GACCTTCCGC TTCTTCTTTG

 9781 GGGCCATCTT ATCGTCATCG TCTTTGTAAT CAATATCATG ATCCTTGTAG TCTCCGTCGT

 9841 GGTCCTTATA GTCCATGGTG GCACCGGTCC AACCTGAAAA AAAGTGATTT CAGGCAGGTG

 9901 CTCCAGGTAA TTAAACATTA ATACCCCACC AACCAACCAT CCCTTAAACC CTTACCTCTT

 9961 GCTCAGCTAA TTACAGCCCG GAGGAGAAGG GCCGTCCCGC CCGCTCACCT GTGGGAGTAA

10021 CGCGGTCAGT CAGAGCCGGG GCGGGCGGCG CGAGGCGGCG GCGGAGCGGG GCACGGGGCG

10081 AAGGCAGCGC GCAGCGACTC CCGCCCGCCG CGCGCTTCGC TTTTTATAGG GCCGCCGCCG

10141 CCGCCGCCTC GCCATAAAAG GAAACTTTCG GAGCGCGCCG CTCTGATTGG CTGCCGCCGC

10201 ACC

//


PX260 载体其他相关CRISPR/Cas9载体

PX856

PX552

PX855

PX551

PX854

PX544

PX853

PX543

PX852

PX462

pX851

PX461

pX603

PX460

pX602

PX459

pX601

PX458

pX600

PX429

PX399

PX387

PX398

PX386

PX396

PX385

PX395

PX383

PX393

PX382

PX392

PX381

PX391

PX380

PX390

PX379

PX389

PX378

PX388

PX377

PX335

pWJ66

PX334

pGuide-it-ZsGreen1

PX333

pGuide-it-tdTomato

PX330S-2

pgRNA-humanized

pX330A-1x2

pgRNA-bacteria

PX330

pFGC-pcoCas9

PX260

pET-NLS-Cas9-6xHis

PX165

pET-Cas9-NLS-6xHis

pwtCas9-bacteria

pET-Cas9-6xHis

pWJ68

pET-28b-Cas9-His

pdCas9-humanized

pCas9D10A-GFP

pCas-Guide-Nickase

pCas9

pCas-Guide-EF1a-GFP

pCas

pCas-Guide-EF1a-CD4

lentiCRISPR v2

pCas-Guide-AAVS1

lenti dCAS-VP64_Blast

pCas-Guide

eSpCas9(1.1)

pCas9-GFP