PX260
PX260
编号 | 载体名称 |
北京华越洋VECT75253 | PX260 |
PX260 载体基本信息:
载体名称: | PX260 或 pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro |
质粒类型: | CRISPR-Cas9载体;基因敲除载体;哺乳动物载体 |
高拷贝/低拷贝: | 高拷贝 |
插入位点: | 限制性内切酶,多克隆位点 |
启动子: | U6 |
载体大小: | 10203bp |
5' 测序引物及序列: | LKO.1 5':GACTATCATATGCTTACCGT |
3' 测序引物及序列: | BGH Reverse:TAGAAGGCACAGTCGAGG |
载体标签: | 3xFLAG(N ter) |
载体抗性: | 氨苄青霉素 |
筛选标记: | Puromycin |
克隆菌株: | DH5alpha |
宿主细胞(系): | 常规哺乳动物细胞等 |
备注: | humanized S. pyogenes Cas9; This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA. |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
PX260 载体质粒图谱和多克隆位点信息:
PX260 载体序列:
ORIGIN
1 TCTCCGCCTC GCCCCGCCCC GCCCCTCGCC CCGCCCCCCC CCCCCCCCCG CCCCCATCGC
61 TGCACAAAAT AATTAAAAAA TAAATAAATA CAAAATTGGG GGTGGGGAGG GGGGGGAGAT
121 GGGGAGAGTG AAGCAGAACG TGGGGCTCAC CTCGACCATG GTAATAGCGA TGACTAATAC
181 GTAGATGTAC TGCCAAGTAG GAAAGTCCCA TAAGGTCATG TACTGGGCAC AATGCCAGGC
241 GGGCCATTTA CCGTCATTGA CGTCAATAGG GGGCGTACTT GGCATATGAT ACACTTGATG
301 TACTGCCAAG TGGGCAGTTT ACCGTAAATA CTCCACCCAT TGACGTCAAT GGAAAGTCCC
361 TATTGGCGTT ACTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC
421 ATTTACCGTA AGTTATGTAA CGGGTACCTC TAGAGCCATT TGTCTGCAGA ATTGGCGCAC
481 GCGCTAGAAA AAGTTTTGGG ACCATTCAAA ACAGCATAGC TCTAAAACGT CTTCTCGAAG
541 ACCCGTTTTG GGACCATTCA AAACAGCATA GCTCTAAAAC CCGGTGTTTC GTCCTTTCCA
601 CAAGATATAT AAAGCCAAGA AATCGAAATA CTTTCAAGTT ACGGTAAGCA TATGATAGTC
661 CATTTTAAAA CATAATTTTA AAACTGCAAA CTACCCAAGA AATTATTACT TTCTACGTCA
721 CGTATTTTGT ACTAATATCT TTGTGTTTAC AGTCAAATTA ATTCCAATTA TCTCTCTAAC
781 AGCCTTGTAT CGTATATGCA AATATGAAGG AATCATGGGA AATAGGCCCT CACATGTGAG
841 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
901 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
961 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
1021 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
1081 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
1141 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
1201 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
1261 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
1321 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
1381 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
1441 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
1501 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
1561 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
1621 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
1681 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
1741 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGGCTTCCAC
1801 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
1861 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
1921 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
1981 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
2041 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
2101 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
2161 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
2221 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
2281 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
2341 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
2401 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
2461 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
2521 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
2581 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
2641 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
2701 GGCCCTTTCG TCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC
2761 CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG
2821 CGTCAGCGGG TGTTGGCGGG TGTCGGGGCT GGCTTAACTA TGCGGCATCA GAGCAGATTG
2881 TACTGAGAGT GCACCATAAA ATTGTAAACG TTAATATTTT GTTAAAATTC GCGTTAAATT
2941 TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGACCGAAAT CGGCAAAATC CCTTATAAAT
3001 CAAAAGAATA GCCCGAGATA GAGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT
3061 TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC
3121 TACGTGAACC ATCACCCAAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC
3181 GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA
3241 GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAAGGC GCTGGCAAGT GTAGCGGTCA
3301 CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTACTATG
3361 GTTGCTTTGA CGTATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
3421 CAGGCGCCGC TCCAATCCTT CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC
3481 AAAGTATGAC AGGAAAAACT TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA
3541 ATTTCTGCAT CCCGGGGATC TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC
3601 GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT TGCGGGTCAT GCACCAGGTG
3661 CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA AGCCGAGCCG CTCGTAGAAG
3721 GGGAGGTTGT GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA CCCCGGCGCG CTCGGCCGCC
3781 TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC CCTGGTGGTC GGGCGAGACG
3841 CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT GCGGCGCCAG GAGGCCTTCC
3901 ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG CCATGCGCGG GCCGATCTCG
3961 GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC AGACCGCCAC CGCGGCGCCG
4021 TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC GCGTGAGGAA GAGTTCTTGC
4081 AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGATCGACGG TGTGGCGCGT GGCGGGGTAG
4141 TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCTGGGGA CGTCGTCGCG GGTGGCGAGG
4201 CGCACCGTGG GCTTGTACTC GGTCATGGTA AGCTTCAGCT GCTCGAGATC TAGATGGATG
4261 CAGGTCGAAA GGCCCGGAGA TGAGGAAGAG GAGAACAGCG CGGCAGACGT GCGCTTTTGA
4321 AGCGTGCAGA ATGCCGGGCC TCCGGAGGAC CTTCGGGCGC CCGCCCCGCC CCTGAGCCCG
4381 CCCCTGAGCC CGCCCCCGGA CCCACCCCTT CCCAGCCTCT GAGCCCAGAA AGCGAAGGAG
4441 CAAAGCTGCT ATTGGCCGCT GCCCCAAAGG CCTACCCGCT TCCATTGCTC AGCGGTGCTG
4501 TCCATCTGCA CGAGACTAGT GAGACGTGCT ACTTCCATTT GTCACGTCCT GCACGACGCG
4561 AGCTGCGGGG CGGGGGGGAA CTTCCTGACT AGGGGAGGAG TAGAAGGTGG CGCGAAGGGG
4621 CCACCAAAGA ACGGAGCCGG TTGGCGCCTA CCGGTGGATG TGGAATGTGT GCGAGGCCAG
4681 AGGCCACTTG TGTAGCGCCA AGTGCCCAGC GGGGCTGCTA AAGCGCATGC TCCAGACTGC
4741 CTTGGGAAAA GCGCCTCCCC TACCCGGTAG AATTTCGAGG TCGAGATCCT AAGCTTGGCT
4801 GGACGTAAAC TCCTCTTCAG ACCTAATAAC TTCGTATAGC ATACATTATA CGAAGTTATA
4861 TTAAGGGTTA TTGAATATGA TCGGAATTCT TACTTGTACA GCTCGTCCAT GCCGAGAGTG
4921 ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT
4981 TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG CGCCAAAAAG CACCGACTCG
5041 GTGCCACTTT TTCAAGTTGA TAACGGACTA GCCTTATTTT AACTTGCTAT GCTGTTTTGA
5101 ATGGTTCCAA CTAGATCTGT GGTCTCATAC AGAACTTATA AGATTCCCAA ATCCAAAGAC
5161 ATTTCACGTT TATGGTGATT TCCCAGAACA CATAGCGACA TGCAAATATT GCAGGGCGCC
5221 ACTCCCCTGT CCCTCACAGC CATCTTCCTG CCAGGGCGCA CGCGCGCTGG GTGTTCCCGC
5281 CTAGTGACAC TGGGCCCGCG ATTCCTTGGA GCGGGTTGAT GACGTCAGCG TTCGAATTGC
5341 GGCCGCTCCC CAGCATGCCT GCTATTCTCT TCCCAATCCT CCCCCTTGCT GTCCTGCCCC
5401 ACCCCACCCC CCAGAATAGA ATGACACCTA CTCAGACAAT GCGATGCAAT TTCCTCATTT
5461 TATTAGGAAA GGACAGTGGG AGTGGCACCT TCCAGGGTCA AGGAAGGCAC GGGGGAGGGG
5521 CAAACAACAG ATGGCTGGCA ACTAGAAGGC ACAGTCGAGG CTGATCAGCG AGCTCTAGGA
5581 ATTCTTACTT TTTCTTTTTT GCCTGGCCGG CCTTTTTCGT GGCCGCCGGC CTTTTGTCGC
5641 CTCCCAGCTG AGACAGGTCG ATCCGTGTCT CGTACAGGCC GGTGATGCTC TGGTGGATCA
5701 GGGTGGCGTC CAGCACCTCT TTGGTGCTGG TGTACCTCTT CCGGTCGATG GTGGTGTCAA
5761 AGTACTTGAA GGCGGCAGGG GCTCCCAGAT TGGTCAGGGT AAACAGGTGG ATGATATTCT
5821 CGGCCTGCTC TCTGATGGGC TTATCCCGGT GCTTGTTGTA GGCGGACAGC ACTTTGTCCA
5881 GATTAGCGTC GGCCAGGATC ACTCTCTTGG AGAACTCGCT GATCTGCTCG ATGATCTCGT
5941 CCAGGTAGTG CTTGTGCTGT TCCACAAACA GCTGTTTCTG CTCATTATCC TCGGGGGAGC
6001 CCTTCAGCTT CTCATAGTGG CTGGCCAGGT ACAGGAAGTT CACATATTTG GAGGGCAGGG
6061 CCAGTTCGTT TCCCTTCTGC AGTTCGCCGG CAGAGGCCAG CATTCTCTTC CGGCCGTTTT
6121 CCAGCTCGAA CAGGGAGTAC TTAGGCAGCT TGATGATCAG GTCCTTTTTC ACTTCTTTGT
6181 AGCCCTTGGC TTCCAGAAAG TCGATGGGAT TCTTCTCGAA GCTGCTTCTT TCCATGATGG
6241 TGATCCCCAG CAGCTCTTTC ACACTCTTCA GTTTCTTGGA CTTGCCCTTT TCCACTTTGG
6301 CCACCACCAG CACAGAATAG GCCACGGTGG GGCTGTCGAA GCCGCCGTAC TTCTTAGGGT
6361 CCCAGTCCTT CTTTCTGGCG ATCAGCTTAT CGCTGTTCCT CTTGGGCAGG ATAGACTCTT
6421 TGCTGAAGCC GCCTGTCTGC ACCTCGGTCT TTTTCACGAT ATTCACTTGG GGCATGCTCA
6481 GCACTTTCCG CACGGTGGCA AAATCCCGGC CCTTATCCCA CACGATCTCC CCGGTTTCGC
6541 CGTTTGTCTC GATCAGAGGC CGCTTCCGGA TCTCGCCGTT GGCCAGGGTA ATCTCGGTCT
6601 TGAAAAAGTT CATGATGTTG CTGTAGAAGA AGTACTTGGC GGTAGCCTTG CCGATTTCCT
6661 GCTCGCTCTT GGCGATCATC TTCCGCACGT CGTACACCTT GTAGTCGCCG TACACGAACT
6721 CGCTTTCCAG CTTAGGGTAC TTTTTGATCA GGGCGGTTCC CACGACGGCG TTCAGGTAGG
6781 CGTCGTGGGC GTGGTGGTAG TTGTTGATCT CGCGCACTTT GTAAAACTGG AAATCCTTCC
6841 GGAAATCGGA CACCAGCTTG GACTTCAGGG TGATCACTTT CACTTCCCGG ATCAGCTTGT
6901 CATTCTCGTC GTACTTAGTG TTCATCCGGG AGTCCAGGAT CTGTGCCACG TGCTTTGTGA
6961 TCTGCCGGGT TTCCACCAGC TGTCTCTTGA TGAAGCCGGC CTTATCCAGT TCGCTCAGGC
7021 CGCCTCTCTC GGCCTTGGTC AGATTGTCGA ACTTTCTCTG GGTAATCAGC TTGGCGTTCA
7081 GCAGCTGCCG CCAGTAGTTC TTCATCTTCT TCACGACCTC TTCGGAGGGC ACGTTGTCGC
7141 TCTTGCCCCG GTTCTTGTCG CTTCTGGTCA GCACCTTGTT GTCGATGGAG TCGTCCTTCA
7201 GAAAGCTCTG AGGCACGATA TGGTCCACAT CGTAGTCGGA CAGCCGGTTG ATGTCCAGTT
7261 CCTGGTCCAC GTACATATCC CGCCCATTCT GCAGGTAGTA CAGGTACAGC TTCTCGTTCT
7321 GCAGCTGGGT GTTTTCCACG GGGTGTTCTT TCAGGATCTG GCTGCCCAGC TCTTTGATGC
7381 CCTCTTCGAT CCGCTTCATT CTCTCGCGGC TGTTCTTCTG TCCCTTCTGG GTGGTCTGGT
7441 TCTCTCTGGC CATTTCGATC ACGATGTTCT CGGGCTTGTG CCGGCCCATC ACTTTCACGA
7501 GCTCGTCCAC CACCTTCACT GTCTGCAGGA TGCCCTTCTT AATGGCGGGG CTGCCGGCCA
7561 GATTGGCAAT GTGCTCGTGC AGGCTATCGC CCTGGCCGGA CACCTGGGCT TTCTGGATGT
7621 CCTCTTTAAA GGTCAGGCTG TCGTCGTGGA TCAGCTGCAT GAAGTTTCTG TTGGCGAAGC
7681 CGTCGGACTT CAGGAAATCC AGGATTGTCT TGCCGGACTG CTTGTCCCGG ATGCCGTTGA
7741 TCAGCTTCCG GCTCAGCCTG CCCCAGCCGG TGTATCTCCG CCGCTTCAGC TGCTTCATCA
7801 CTTTGTCGTC GAACAGGTGG GCATAGGTTT TCAGCCGTTC CTCGATCATC TCTCTGTCCT
7861 CAAACAGTGT CAGGGTCAGC ACGATATCTT CCAGAATGTC CTCGTTTTCC TCATTGTCCA
7921 GGAAGTCCTT GTCCTTGATA ATTTTCAGCA GATCGTGGTA TGTGCCCAGG GAGGCGTTGA
7981 ACCGATCTTC CACGCCGGAG ATTTCCACGG AGTCGAAGCA CTCGATTTTC TTGAAGTAGT
8041 CCTCTTTCAG CTGCTTCACG GTCACTTTCC GGTTGGTCTT GAACAGCAGG TCCACGATGG
8101 CCTTTTTCTG CTCGCCGCTC AGGAAGGCGG GCTTTCTCAT TCCCTCGGTC ACGTATTTCA
8161 CTTTGGTCAG CTCGTTATAC ACGGTGAAGT ACTCGTACAG CAGGCTGTGC TTGGGCAGCA
8221 CCTTCTCGTT GGGCAGGTTC TTATCGAAGT TGGTCATCCG CTCGATGAAG CTCTGGGCGG
8281 AAGCGCCCTT GTCCACCACT TCCTCGAAGT TCCAGGGGGT GATGGTTTCC TCGCTCTTTC
8341 TGGTCATCCA GGCGAATCTG CTGTTTCCCC TGGCCAGAGG GCCCACGTAG TAGGGGATGC
8401 GGAAGGTCAG GATCTTCTCG ATCTTTTCCC GGTTGTCCTT CAGGAATGGG TAAAAATCTT
8461 CCTGCCGCCG CAGAATGGCG TGCAGCTCTC CCAGGTGGAT CTGGTGGGGG ATGCTGCCGT
8521 TGTCGAAGGT CCGCTGCTTC CGCAGCAGGT CCTCTCTGTT CAGCTTCACG AGCAGTTCCT
8581 CGGTGCCGTC CATCTTTTCC AGGATGGGCT TGATGAACTT GTAGAACTCT TCCTGGCTGG
8641 CTCCGCCGTC AATGTAGCCG GCGTAGCCGT TCTTGCTCTG GTCGAAGAAA ATCTCTTTGT
8701 ACTTCTCAGG CAGCTGCTGC CGCACGAGAG CTTTCAGCAG GGTCAGGTCC TGGTGGTGCT
8761 CGTCGTATCT CTTGATCATA GAGGCGCTCA GGGGGGCCTT GGTGATCTCG GTGTTCACTC
8821 TCAGGATGTC GCTCAGCAGG ATGGCGTCGG ACAGGTTCTT GGCGGCCAGA AACAGGTCGG
8881 CGTACTGGTC GCCGATCTGG GCCAGCAGGT TGTCCAGGTC GTCGTCGTAG GTGTCCTTGC
8941 TCAGCTGCAG TTTGGCATCC TCGGCCAGGT CGAAGTTGCT CTTGAAGTTG GGGGTCAGGC
9001 CCAGGCTCAG GGCAATCAGG TTTCCGAACA GGCCATTCTT CTTCTCGCCG GGCAGCTGGG
9061 CGATCAGATT TTCCAGCCGT CTGCTCTTGC TCAGTCTGGC AGACAGGATG GCCTTGGCGT
9121 CCACGCCGCT GGCGTTGATG GGGTTTTCCT CGAACAGCTG GTTGTAGGTC TGCACCAGCT
9181 GGATGAACAG CTTGTCCACG TCGCTGTTGT CGGGGTTCAG GTCGCCCTCG ATCAGGAAGT
9241 GGCCCCGGAA CTTGATCATG TGGGCCAGGG CCAGATAGAT CAGCCGCAGG TCGGCCTTGT
9301 CGGTGCTGTC CACCAGTTTC TTTCTCAGGT GGTAGATGGT GGGGTACTTC TCGTGGTAGG
9361 CCACCTCGTC CACGATGTTG CCGAAGATGG GGTGCCGCTC GTGCTTCTTA TCCTCTTCCA
9421 CCAGGAAGGA CTCTTCCAGT CTGTGGAAGA AGCTGTCGTC CACCTTGGCC ATCTCGTTGC
9481 TGAAGATCTC TTGCAGATAG CAGATCCGGT TCTTCCGTCT GGTGTATCTT CTTCTGGCGG
9541 TTCTCTTCAG CCGGGTGGCC TCGGCTGTTT CGCCGCTGTC GAACAGCAGG GCTCCGATCA
9601 GGTTCTTCTT GATGCTGTGC CGGTCGGTGT TGCCCAGCAC CTTGAATTTC TTGCTGGGCA
9661 CCTTGTACTC GTCGGTGATC ACGGCCCAGC CCACAGAGTT GGTGCCGATG TCCAGGCCGA
9721 TGCTGTACTT CTTGTCGGCT GCTGGGACTC CGTGGATACC GACCTTCCGC TTCTTCTTTG
9781 GGGCCATCTT ATCGTCATCG TCTTTGTAAT CAATATCATG ATCCTTGTAG TCTCCGTCGT
9841 GGTCCTTATA GTCCATGGTG GCACCGGTCC AACCTGAAAA AAAGTGATTT CAGGCAGGTG
9901 CTCCAGGTAA TTAAACATTA ATACCCCACC AACCAACCAT CCCTTAAACC CTTACCTCTT
9961 GCTCAGCTAA TTACAGCCCG GAGGAGAAGG GCCGTCCCGC CCGCTCACCT GTGGGAGTAA
10021 CGCGGTCAGT CAGAGCCGGG GCGGGCGGCG CGAGGCGGCG GCGGAGCGGG GCACGGGGCG
10081 AAGGCAGCGC GCAGCGACTC CCGCCCGCCG CGCGCTTCGC TTTTTATAGG GCCGCCGCCG
10141 CCGCCGCCTC GCCATAAAAG GAAACTTTCG GAGCGCGCCG CTCTGATTGG CTGCCGCCGC
10201 ACC
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PX260 载体其他相关CRISPR/Cas9载体: