pCas9
pCas9
编号 | 载体名称 |
北京华越洋VECT75255 | pCas9 |
pCas9载体基本信息：
载体名称: | pCas9 |
质粒类型: | Crisper/cas9 载体，用于敲除大肠杆菌基因 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 限制性内切酶，多克隆位点 |
启动子: | p15a |
载体大小: | 9.3 kb |
5' 测序引物及序列: | -- |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氯霉素 |
克隆菌株: | DH5α 等 |
宿主细胞（系）: | 常规大肠杆菌 |
备注: | 用于常规大肠杆菌的基因crisper/cas9技术基因敲除 |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pCas9载体质粒图谱和多克隆位点信息：
pCas9载体序列：
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
1681 attacgaaat catcctgtgg agcttagtag gtttagcaag atggcagcgc ctaaatgtag
1741 aatgataaaa ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag
1801 cgcttgtttt tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc
1861 ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg
1921 aatggttcca acaagattat tttataactt ttataacaaa taatcaagga gaaattcaaa
1981 gaaatttatc agccataaaa caatacttaa tactatagaa tgataacaaa ataaactact
2041 ttttaaaaga attttgtgtt ataatctatt tattattaag tattgggtaa tattttttga
2101 agagatattt tgaaaaagaa aaattaaagc atattaaact aatttcggag gtcattaaaa
2161 ctattattga aatcatcaaa ctcattatgg atttaattta aactttttat tttaggaggc
2221 aaaaatggat aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc
2281 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
2341 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
2401 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
2461 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
2521 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
2581 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
2641 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
2701 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
2761 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa
2821 ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc
2881 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg
2941 gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc
3001 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt
3061 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc
3121 tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc
3181 ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt
3241 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata
3301 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat
3361 tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct
3421 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct
3481 gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa
3541 gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa
3601 tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga
3661 agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga
3721 taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac
3781 ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt
3841 tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt
3901 aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga
3961 aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa
4021 aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat
4081 tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata
4141 tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg
4201 gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat
4261 attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga
4321 tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag
4381 tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca
4441 gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat
4501 cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga
4561 gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca
4621 tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg
4681 aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga
4741 tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg
4801 ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat
4861 gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa
4921 tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg
4981 ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat
5041 gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa
5101 atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa
5161 caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa
5221 gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg
5281 taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta
5341 ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa
5401 acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga
5461 ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga
5521 agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct
5581 tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt
5641 agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc
5701 cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat
5761 tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc
5821 taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga
5881 attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc
5941 tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt
6001 ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg
6061 tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga
6121 caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg
6181 agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac
6241 aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg
6301 cattgatttg agtcagctag gaggtgactg aagtatattt tagatgaaga ttatttctta
6361 ataactaaaa atatggtata atactcttaa taaatgcagt aatacagggg cttttcaaga
6421 ctgaagtcta gctgagacaa atagtgcgat tacgaaattt tttagacaaa aatagtctac
6481 gaggttttag agctatgctg ttttgaatgg tcccaaaact gagaccagtc tcggaagctc
6541 aaaggtctcg ttttagagct atgctgtttt gaatggtccc aaaacttcag cacactgaga
6601 cttgttgagt tccatgtttt agagctatgc tgttttgaat ggactccatt caacattgcc
6661 gatgataact tgagaaagag ggttaatacc agcagtcgga taccttccta ttctttctgt
6721 taaagcgttt tcatgttata ataggcaaaa gaagagtagt gtgatcgtcc attccgacag
6781 catcgccagt cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc
6841 acccgttctc ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct
6901 acttggagcc actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta
6961 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
7021 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
7081 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
7141 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
7201 aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt
7261 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
7321 tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg
7381 ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc
7441 cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat
7501 tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg
7561 ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt
7621 gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct
7681 cgcggctctt accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc
7741 cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt
7801 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc
7861 cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg
7921 agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc
7981 agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc
8041 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
8101 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
8161 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc
8221 cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata
8281 ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc
8341 gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
8401 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc
8461 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc
8521 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
8581 aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta
8641 ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca
8701 cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac
8761 gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg
8821 cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa
8881 aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
8941 ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc
9001 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
9061 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
9121 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
9181 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
9241 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
9301 agctcaccgt ctttcattgc catacg
//
pCas9载体其他相关CRISPR/Cas9载体：
