北京华越洋生物
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首页    载体及质粒    pBABE-neo
pBABE-neoMap

pBABE-neo

pBABE-neo

pBABE-neo

 



编号

载体名称

北京华越洋VECT55164

pBABE-neo

 

pBABE-neo逆病毒载体基本信息:

载体名称:

pBABE-neo

质粒类型:

逆病毒载体

高拷贝/低拷贝:

高拷贝

克隆方法:

限制性内切酶,多克隆位点

启动子:

MSCV

载体大小:

5330 bp

5' 测序引物及序列:

pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'

3' 测序引物及序列:

pBABE 3' primer   5'ACCCTAACTGACACACATTCC3'

载体标签:

载体抗性:

氨苄青霉素

筛选标记:

Neomycin

克隆菌株:

DH5αHB101

宿主细胞(系):

常规哺乳动物细胞系

备注:


 
推荐使用逆病毒包装载体:pUMVCpCMV-VSV-G
   

稳定性:

稳表达

组成型/诱导型:

组成型

病毒/非病毒:

逆转录病毒

 

pBABE-neo载体质粒图谱和多克隆位点信息:

 

 

pBABE-neo载体简介

pBABE-neo载体序列:

ORIGIN

    1 CTGCAGCCTG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA

   61 GGGCCAAGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT

  121 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCAG CCCTCAGCAG

  181 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC

  241 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA

  301 GCTCAATAAA AGAGCCCACA ACCCCTCACT CGGGGCGCCA GTCCTCCGAT TGACTGAGTC

  361 GCCCGGGTAC CCGTGTATCC AATAAACCCT CTTGCAGTTG CATCCGACTT GTGGTCTCGC

  421 TGTTCCTTGG GAGGGTCTCC TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTTCATTT

  481 GGGGGCTCGT CCGGGATCGG GAGACCCCTG CCCAGGGACC ACCGACCCAC CACCGGGAGG

  541 TAAGCTGGCC AGCAACTTAT CTGTGTCTGT CCGATTGTCT AGTGTCTATG ACTGATTTTA

  601 TGCGCCTGCG TCGGTACTAG TTAGCTAACT AGCTCTGTAT CTGGCGGACC CGTGGTGGAA

  661 CTGACGAGTT CTGAACACCC GGCCGCAACC CTGGGAGACG TCCCAGGGAC TTTGGGGGCC

  721 GTTTTTGTGG CCCGACCTGA GGAAGGGAGT CGATGTGGAA TCCGACCCCG TCAGGATATG

  781 TGGTTCTGGT AGGAGACGAG AACCTAAAAC AGTTCCCGCC TCCGTCTGAA TTTTTGCTTT

  841 CGGTTTGGAA CCGAAGCCGC GCGTCTTGTC TGCTGCAGCA TCGTTCTGTG TTGTCTCTGT

  901 CTGACTGTGT TTCTGTATTT GTCTGAAAAT TAGGGCCAGA CTGTTACCAC TCCCTTAAGT

  961 TTGACCTTAG ATCACTGGAA AGATGTCGAG CGGCTCGCTC ACAACCAGTC GGTAGATGTC

 1021 AAGAAGAGAC GTTGGGTTAC CTTCTGCTCT GCAGAATGGC CAACCTTTAA CGTCGGATGG

 1081 CCGCGAGACG GCACCTTTAA CCGAGACCTC ATCACCCAGG TTAAGATCAA GGTCTTTTCA

 1141 CCTGGCCCGC ATGGACACCC AGACCAGGTC CCCTACATCG TGACCTGGGA AGCCTTGGCT

 1201 TTTGACCCCC CTCCCTGGGT CAAGCCCTTT GTACACCCTA AGCCTCCGCC TCCTCTTCTT

 1261 CCATCCGCGC CGTCTCTCCC CCTTGAACCT CCTCTTTCGA CCCCGCCTCA ATCCTCCCTT

 1321 TATCCAGCCC TCACTCCTTC TCTAGGCGCC GGCCGGATCC CAGTGTGGTG GTACGTAGGA

 1381 ATTCGCCAGC ACAGTGGTCG ACCCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC

 1441 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT

 1501 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT

 1561 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG

 1621 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT

 1681 CGGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA

 1741 AAAAGCTTAC CATGACCGAG TACAAGCCCA TGATTGAACA AGATGGATTG CACGCAGGTT

 1801 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT

 1861 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA

 1921 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG

 1981 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT

 2041 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG

 2101 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT

 2161 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG

 2221 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT

 2281 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG

 2341 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC

 2401 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG

 2461 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT

 2521 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGATCGA TAAAATAAAA

 2581 GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC CCCACCTGTA GGTTTGGCAA

 2641 GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAGAA

 2701 GTTCAGATCA AGGTCAGGAA CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT

 2761 GGTAAGCAGT TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC

 2821 AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC

 2881 CCAGATGCGG TCCAGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC

 2941 CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT

 3001 CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGGG

 3061 CGCCAGTCCT CCGATTGACT GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC

 3121 AGTTGCATCC GACTTGTGGT CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT

 3181 ACCCGTCAGC GGGGGTCTTT CATTTCCGAC TTGTGGTCTC GCTGCCTTGG GAGGGTCTCC

 3241 TCTGAGTGAT TGACTACCCG TCAGCGGGGG TCTTCACATG CAGCATGTAT CAAAATTAAT

 3301 TTGGTTTTTT TTCTTAAGTA TTTACATTAA ATGGCCATAG TTGCATTAAT GAATCGGCCA

 3361 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG

 3421 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG

 3481 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG

 3541 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC

 3601 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA

 3661 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT

 3721 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC

 3781 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC

 3841 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA

 3901 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT

 3961 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA

 4021 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT

 4081 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT

 4141 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT

 4201 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC

 4261 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTGCGGCCGC AAATCAATCT AAAGTATATA

 4321 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT

 4381 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG

 4441 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC

 4501 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC

 4561 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC

 4621 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC

 4681 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC

 4741 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA

 4801 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT

 4861 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA

 4921 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAACA CGGGATAATA CCGCGCCACA

 4981 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG

 5041 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC

 5101 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC

 5161 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA

 5221 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA

 5281 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC

//

 

pBABE-neo其他相关逆病毒载体:

pVSV-G

pBABE

pBABE-hygro-hTERT

pBABE-neo-SV40LT

pMKO.1-GFP

pBABE-neo

pBABE-GFP

pVPack-10A1

pBABE-Puro

pCMV-Gag-Pol

pVPack-Eco

pCMV-VSV-G

pCL-Eco

pCMV-VSV-G

pMSCV-PIG

pFB-ERV

pFB-hrGFP

pFB-Neo

pCFB

pRetroX-Tight-Pur-Luc

pRetroX-TetOne

pRetroX-PTuner2

pRetroX-PTuner-IRES

pRetroX-TRE3G-Luc

pRetroX-Tet-On     Advanced

pRetroQ-mCherry-N1

pRetroX-Tight-Pur

pRetroQ-AcGFP1-C1

pRetroQ-DsRed-Monomer-C1

RNAi-Ready     pSIREN-RetroQ-DsRed-Express

pRetroX-SG2M-Cyan

pQCXIX

pLXSN

RNAi-Ready     pSIREN-RetroQ-ZsGreen1

PSUPER.RETRO.PURO

pMSCVhyg

PRETROX-IRES-ZSGREEN1

pSuper.Retro.Neo

pMSCVpuro

pMKO.1-puro

pMSCV-FLIP-puro-dsRed-GFP-miRNA

pBABE-HAII

pBABE-puro-IRES-EGFP

pBABE-Puro     SV40LT

pBABE-neo-hTERT

pBABE-zeo

pBABE-hygro

pVPack-VSV-G

pVPack-GP

pCL-Ampho

pVPack-Ampho

pCMV-VSVG

pUMVC

pCFB-EGSH

pQCXIN

pFB

pFB-Luc

pRetroX-TetOne-luc

pRetro-Lib

pRetroX-PTuner2-C

pRetroX-PTuner

pRetroX-Tet3G

pRetroX-TRE3G

pRetroX-Tight-Hyg-Luc

pRetroX-Tet-Off     Advanced

pRetroQ-mCherry-C1

pRetroX-G1-Red

pRetroQ-AcGFP1-N1

pRetroQ-DsRed-Monomer-N1

pLXIN

pLNCX2

pSuper.Retro.Neo+GFP

pSilencer5.1-U6     Retro

pRetroX-IRES-DsRedExpress

RNAi-Ready     pSIREN-RetroQ

pMSCVneo

pQCXIH

pSilencer5.1-H1     Retro

pCLXSN