pCMV-VSVG
pCMV-VSVG
编号 | 载体名称 |
北京华越洋VECT55187 | pCMV-VSVG |
pCMV-VSVG逆病毒载体基本信息：
载体名称: | pCMV-VSVG |
质粒类型: | 逆转录病毒包装载体；封闭载体 |
高拷贝/低拷贝: | 高拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 6363 |
5' 测序引物及序列: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
3' 测序引物及序列: | -- |
载体标签: | -- |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
备注: | |
稳定性: | 稳表达 |
组成型: | 组成型 |
病毒/非病毒: | 逆病毒 |
pCMV-VSVG载体质粒图谱和多克隆位点信息：
pCMV-VSVG载体简介
pCMV-VSVG载体序列：
ORIGIN
1 GAGCTTGGCC CATTGCATAC GTTGTATCCA TATCATAATA TGTACATTTA TATTGGCTCA
61 TGTCCAACAT TACCGCCATG TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT
121 ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT
181 GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT
241 CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA
301 ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC
361 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT
421 ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG
481 TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT
541 GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC
601 AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
661 AGAGCTCGTT TAGTGAACCG TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC
721 CATAGAAGAC ACCGGGACCG ATCCAGCCTC CGGTCGACCG ATCCTGAGAA CTTCAGGGTG
781 AGTTTGGGGA CCCTTGATTG TTCTTTCTTT TTCGCTATTG TAAAATTCAT GTTATATGGA
841 GGGGGCAAAG TTTTCAGGGT GTTGTTTAGA ATGGGAAGAT GTCCCTTGTA TCACCATGGA
901 CCCTCATGAT AATTTTGTTT CTTTCACTTT CTACTCTGTT GACAACCATT GTCTCCTCTT
961 ATTTTCTTTT CATTTTCTGT AACTTTTTCG TTAAACTTTA GCTTGCATTT GTAACGAATT
1021 TTTAAATTCA CTTTTGTTTA TTTGTCAGAT TGTAAGTACT TTCTCTAATC ACTTTTTTTT
1081 CAAGGCAATC AGGGTATATT ATATTGTACT TCAGCACAGT TTTAGAGAAC AATTGTTATA
1141 ATTAAATGAT AAGGTAGAAT ATTTCTGCAT ATAAATTCTG GCTGGCGTGG AAATATTCTT
1201 ATTGGTAGAA ACAACTACAC CCTGGTCATC ATCCTGCCTT TCTCTTTATG GTTACAATGA
1261 TATACACTGT TTGAGATGAG GATAAAATAC TCTGAGTCCA AACCGGGCCC CTCTGCTAAC
1321 CATGTTCATG CCTTCTTCTC TTTCCTACAG CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG
1381 TCTCATCATT TTGGCAAAGA ATTCCTCGAC GGATCCGCCA TGAAGTGCCT TTTGTACTTA
1441 GCCTTTTTAT TCATTGGGGT GAATTGCAAG TTCACCATAG TTTTTCCACA CAACCAAAAA
1501 GGAAACTGGA AAAATGTTCC TTCTAATTAC CATTATTGCC CGTCAAGCTC AGATTTAAAT
1561 TGGCATAATG ACTTAATAGG CACAGCCTTA CAAGTCAAAA TGCCCAAGAG TCACAAGGCT
1621 ATTCAAGCAG ACGGTTGGAT GTGTCATGCT TCCAAATGGG TCACTACTTG TGATTTCCGC
1681 TGGTATGGAC CGAAGTATAT AACACATTCC ATCCGATCCT TCACTCCATC TGTAGAACAA
1741 TGCAAGGAAA GCATTGAACA AACGAAACAA GGAACTTGGC TGAATCCAGG CTTCCCTCCT
1801 CAAAGTTGTG GATATGCAAC TGTGACGGAT GCCGAAGCAG TGATTGTCCA GGTGACTCCT
1861 CACCATGTGC TGGTTGATGA ATACACAGGA GAATGGGTTG ATTCACAGTT CATCAACGGA
1921 AAATGCAGCA ATTACATATG CCCCACTGTC CATAACTCTA CAACCTGGCA TTCTGACTAT
1981 AAGGTCAAAG GGCTATGTGA TTCTAACCTC ATTTCCATGG ACATCACCTT CTTCTCAGAG
2041 GACGGAGAGC TATCATCCCT GGGAAAGGAG GGCACAGGGT TCAGAAGTAA CTACTTTGCT
2101 TATGAAACTG GAGGCAAGGC CTGCAAAATG CAATACTGCA AGCATTGGGG AGTCAGACTC
2161 CCATCAGGTG TCTGGTTCGA GATGGCTGAT AAGGATCTCT TTGCTGCAGC CAGATTCCCT
2221 GAATGCCCAG AAGGGTCAAG TATCTCTGCT CCATCTCAGA CCTCAGTGGA TGTAAGTCTA
2281 ATTCAGGACG TTGAGAGGAT CTTGGATTAT TCCCTCTGCC AAGAAACCTG GAGCAAAATC
2341 AGAGCGGGTC TTCCAATCTC TCCAGTGGAT CTCAGCTATC TTGCTCCTAA AAACCCAGGA
2401 ACCGGTCCTG CTTTCACCAT AATCAATGGT ACCCTAAAAT ACTTTGAGAC CAGATACATC
2461 AGAGTCGATA TTGCTGCTCC AATCCTCTCA AGAATGGTCG GAATGATCAG TGGAACTACC
2521 ACAGAAAGGG AACTGTGGGA TGACTGGGCA CCATATGAAG ACGTGGAAAT TGGACCCAAT
2581 GGAGTTCTGA GGACCAGTTC AGGATATAAG TTTCCTTTAT ACATGATTGG ACATGGTATG
2641 TTGGACTCCG ATCTTCATCT TAGCTCAAAG GCTCAGGTGT TCGAACATCC TCACATTCAA
2701 GACGCTGCTT CGCAACTTCC TGATGATGAG AGTTTATTTT TTGGTGATAC TGGGCTATCC
2761 AAAAATCCAA TCGAGCTTGT AGAAGGTTGG TTCAGTAGTT GGAAAAGCTC TATTGCCTCT
2821 TTTTTCTTTA TCATAGGGTT AATCATTGGA CTATTCTTGG TTCTCCGAGT TGGTATCCAT
2881 CTTTGCATTA AATTAAAGCA CACCAAGAAA AGACAGATTT ATACAGACAT AGAGATGAAC
2941 CGACTTGGAA AGTGATAAGG ATCCGTCGAG GAATTCACTC CTCAGGTGCA GGCTGCCTAT
3001 CAGAAGGTGG TGGCTGGTGT GGCCAATGCC CTGGCTCACA AATACCACTG AGATCTTTTT
3061 CCCTCTGCCA AAAATTATGG GGACATCATG AAGCCCCTTG AGCATCTGAC TTCTGGCTAA
3121 TAAAGGAAAT TTATTTTCAT TGCAATAGTG TGTTGGAATT TTTTGTGTCT CTCACTCGGA
3181 AGGACATATG GGAGGGCAAA TCATTTAAAA CATCAGAATG AGTATTTGGT TTAGAGTTTG
3241 GCAACATATG CCCATATGCT GGCTGCCATG AACAAAGGTT GGCTATAAAG AGGTCATCAG
3301 TATATGAAAC AGCCCCCTGC TGTCCATTCC TTATTCCATA GAAAAGCCTT GACTTGAGGT
3361 TAGATTTTTT TTATATTTTG TTTTGTGTTA TTTTTTTCTT TAACATCCCT AAAATTTTCC
3421 TTACATGTTT TACTAGCCAG ATTTTTCCTC CTCTCCTGAC TACTCCCAGT CATAGCTGTC
3481 CCTCTTCTCT TATGGAGATC CCTCGACGGA TCGGCCGCAA TTCGTAATCA TGTCATAGCT
3541 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT
3601 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC
3661 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG
3721 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT
3781 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
3841 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
3901 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
3961 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
4021 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
4081 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG
4141 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
4201 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
4261 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
4321 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT
4381 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
4441 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
4501 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
4561 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC
4621 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC
4681 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT
4741 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT
4801 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT
4861 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC
4921 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA
4981 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG
5041 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT
5101 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC
5161 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT
5221 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG
5281 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC
5341 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC
5401 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT
5461 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG
5521 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG
5581 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA
5641 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTAAATTGT AAGCGTTAAT
5701 ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC
5761 GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT
5821 CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA
5881 ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG
5941 TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA
6001 CGGGGAAAGC CGGCGAACGT GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT
6061 AGGGCGCTGG CAAGTGTAGC GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT
6121 GCGCCGCTAC AGGGCGCGTC CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA
6181 TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA
6241 TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG
6301 CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC ACCGCGGTGG CGGCCGCTCT
6361 AGA
//
pCMV-VSVG其他相关逆病毒载体：
