pVL1393

pVL1393

pVL1393载体基本信息

pVL1393载体质粒图谱和多克隆位点信息

pVL1393载体简介

pVL1393载体和pVL1392载体简介

pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.

Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.

These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.

载体特征

• Multiple cloning site polylinker, positioned in opposite orientations

• Recombination sequences for insertion into the baculovirus genome

• Polyhedrin enhancer-promoter sequences for high expression of recombinant protein

Features for growth and maintenance in E. coli:

• Ampicillin gene for selection

• pMB1 origin of replication from pUC19 for high copy number growth

载体复制

The TOP10 cells are provided as a stab.

TOP10 is provided for growth and maintenance of this plasmid. TOP10 is a recombination-negative strain designed for stable replication of high copy number plasmids. This strain is provided as a convenience for those who do not have access to other E. coli strains. Many E. coli strains are suitable for the growth of this vector including DH5α, DH10, and INVαF'. In general, it is best to grow vectors containing inserts in E. coli strains which are recombination-deficient (recA-).

Genotype of TOP10

F-, mcrA ∆(mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG

pVL1393载体序列

ORIGIN

    1 AAGCTTTACT CGTAAAGCGA GTTGAAGGAT CATATTTAGT TGCGTTTATG AGATAAGATT

   61 GAAAGCACGT GTAAAATGTT TCCCGCGCGT TGGCACAACT ATTTACAATG CGGCCAAGTT

  121 ATAAAAGATT CTAATCTGAT ATGTTTTAAA ACACCTTTGC GGCCCGAGTT GTTTGCGTAC

  181 GTGACTAGCG AAGAAGATGT GTGGACCGCA GAACAGATAG TAAAACAAAA CCCTAGTATT

  241 GGAGCAATAA TCGATTTAAC CAACACGTCT AAATATTATG ATGGTGTGCA TTTTTTGCGG

  301 GCGGGCCTGT TATACAAAAA AATTCAAGTA CCTGGCCAGA CTTTGCCGCC TGAAAGCATA

  361 GTTCAAGAAT TTATTGACAC GGTAAAAGAA TTTACAGAAA AGTGTCCCGG CATGTTGGTG

  421 GGCGTGCACT GCACACACGG TATTAATCGC ACCGGTTACA TGGTGTGCAG ATATTTAATG

  481 CACACCCTGG GTATTGCGCC GCAGGAAGCC ATAGATAGAT TCGAAAAAGC CAGAGGTCAC

  541 AAAATTGAAA GACAAAATTA CGTTCAAGAT TTATTAATTT AATTAATATT ATTTGCATTC

  601 TTTAACAAAT ACTTTATCCT ATTTTCAAAT TGTTGCGCTT CTTCCAGCGA ACCAAAACTA

  661 TGCTTCGCTT GCTCCGTTTA GCTTGTAGCC GATCAGTGGC GTTGTTCCAA TCGACGGTAG

  721 GATTAGGCCG GATATTCTCC ACCACAATGT TGGCAACGTT GATGTTACGT TTATGCTTTT

  781 GGTTTTCCAC GTACGTCTTT TGGCCGGTAA TAGCCGTAAA CGTAGTGCCG TCGCGCGTCA

  841 CGCACAACAC CGGATGTTTG CGCTTGTCCG CGGGGTATTG AACCGCGCGA TCCGACAAAT

  901 CCACCACTTT GGCAACTAAA TCGGTGACCT GCGCGTCTTT TTTCTGCATT ATTTCGTCTT

  961 TCTTTTGCAT GGTTTCCTGG AAGCCGGTGT ACATGCGGTT TAGATCAGTC ATGACGCGCG

 1021 TGACCTGCAA ATCTTTGGCC TCGATCTGCT TGTCCTTGAT GGCAACGATG CGTTCAATAA

 1081 ACTCTTGTTT TTTAACAAGT TCCTCGGTTT TTTGCGCCAC CACCGCTTGC AGCGCGTTTG

 1141 TGTGCTCGGT GAATGTCGCA ATCAGCTTAG TCACCAACTG TTTGCTCTCC TCCTCCCGTT

 1201 GTTTGATCGC GGGATCGTAC TTGCCGGTGC AGAGCACTTG AGGAATTACT TCTTCTAAAA

 1261 GCCATTCTTG TAATTCTATG GCGTAAGGCA ATTTGGACTT CATAATCAGC TGAATCACGC

 1321 CGGATTTAGT AATGAGCACT GTATGCGGCT GCAAATACAG CGGGTCGCCC CTTTTCACGA

 1381 CGCTGTTAGA GGTAGGGCCC CCATTTTGGA TGGTCTGCTC AAATAACGAT TTGTATTTAT

 1441 TGTCTACATG AACACGTATA GCTTTATCAC AAACTGTATA TTTTAAACTG TTAGCGACGT

 1501 CCTTGGCCAC GAACCGGACC TGTTGGTCGC GCTCTAGCAC GTACCGCAGG TTGAACGTAT

 1561 CTTCTCCAAA TTTAAATTCT CCAATTTTAA CGCGAGCCAT TTTGATACAC GTGTGTCGAT

 1621 TTTGCAACAA CTATTGTTTT TTAACGCAAA CTAAACTTAT TGTGGTAAGC AATAATTAAA

 1681 TATGGGGGAA CATGCGCCGC TACAACACTC GTCGTTATGA ACGCAGACGG CGCCGGTCTC

 1741 GGCGCAAGCG GCTAAAACGT GTTGCGCGTT CAACGCGGCA AACATCGCAA AAGCCAATAG

 1801 TACAGTTTTG ATTTGCATAT TAACGGCGAT TTTTTAAATT ATCTTATTTA ATAAATAGTT

 1861 ATGACGCCTA CAACTCCCCG CCCGCGTTGA CTCGCTGCAC CTCGAGCAGT TCGTTGACGC

 1921 CTTCCTCCGT GTGGCCGAAC ACGTCGAGCG GGTGGTCGAT GACCAGCGGC GTGCCGCACG

 1981 CGACGCACAA GTATCTGTAC ACCGAATGAT CGTCGGGCGA AGGCACGTCG GCCTCCAAGT

 2041 GGCAATATTG GCAAATTCGA AAATATATAC AGTTGGGTTG TTTGCGCATA TCTATCGTGG

 2101 CGTTGGGCAT GTACGTCCGA ACGTTGATTT GCATGCAAGC CGAAATTAAA TCATTGCGAT

 2161 TAGTGCGATT AAAACGTTGT ACATCCTCGC TTTTAATCAT GCCGTCGATT AAATCGCGCA

 2221 ATCGAGTCAA GTGATCAAAG TGTGGAATAA TGTTTTCTTT GTATTCCCGA GTCAAGCGCA

 2281 GCGCGTATTT TAACAAACTA GCCATCTTGT AAGTTAGTTT CATTTAATGC AACTTTATCC

 2341 AATAATATAT TATGTATCGC ACGTCAAGAA TTAACAATGC GCCCGTTGTC GCATCTCAAC

 2401 ACGACTATGA TAGAGATCAA ATAAAGCGCG AATTAAATAG CTTGCGACGC AACGTGCACG

 2461 ATCTGTGCAC GCGTTCCGGC ACGAGCTTTG ATTGTAATAA GTTTTTACGA AGCGATGACA

 2521 TGACCCCCGT AGTGACAACG ATCACGCCCA AAAGAACTGC CGACTACAAA ATTACCGAGT

 2581 ATGTCGGTGA CGTTAAAACT ATTAAGCCAT CCAATCGACC GTTAGTCGAA TCAGGACCGC

 2641 TGGTGCGAGA AGCCGCGAAG TATGGCGAAT GCATCGTATA ACGTGTGGAG TCCGCTCATT

 2701 AGAGCGTCAT GTTTAGACAA GAAAGCTACA TATTTAATTG ATCCCGATGA TTTTATTGAT

 2761 AAATTGACCC TAACTCCATA CACGGTATTC TACAATGGCG GGGTTTTGGT CAAAATTTCC

 2821 GGACTGCGAT TGTACATGCT GTTAACGGCT CCGCCCACTA TTAATGAAAT TAAAAATTCC

 2881 AATTTTAAAA AACGCAGCAA GAGAAACATT TGTATGAAAG AATGCGTAGA AGGAAAGAAA

 2941 AATGTCGTCG ACATGCTGAA CAACAAGATT AATATGCCTC CGTGTATAAA AAAAATATTG

 3001 AACGATTTGA AAGAAAACAA TGTACCGCGC GGCGGTATGT ACAGGAAGAG GTTTATACTA

 3061 AACTGTTACA TTGCAAACGT GGTTTCGTGT GCCAAGTGTG AAAACCGATG TTTAATCAAG

 3121 GCTCTGACGC ATTTCTACAA CCACGACTCC AAGTGTGTGG GTGAAGTCAT GCATCTTTTA

 3181 ATCAAATCCC AAGATGTGTA TAAACCACCA AACTGCCAAA AAATGAAAAC TGTCGACAAG

 3241 CTCTGTCCGT TTGCTGGCAA CTGCAAGGGT CTCAATCCTA TTTGTAATTA TTGAATAATA

 3301 AAACAATTAT AAATGCTAAA TTTGTTTTTT ATTAACGATA CAAACCAAAC GCAACAAGAA

 3361 CATTTGTAGT ATTATCTATA ATTGAAAACG CGTAGTTATA ATCGCTGAGG TAATATTTAA

 3421 AATCATTTTC AAATGATTCA CAGTTAATTT GCGACAATAT AATTTTATTT TCACATAAAC

 3481 TAGACGCCTT GTCGTCTTCT TCTTCGTATT CCTTCTCTTT TTCATTTTTC TCCTCATAAA

 3541 AATTAACATA GTTATTATCG TATCCATATA TGTATCTATC GTATAGAGTA AATTTTTTGT

 3601 TGTCATAAAT ATATATGTCT TTTTTAATGG GGTGTATAGT ACCGCTGCGC ATAGTTTTTC

 3661 TGTAATTTAC AACAGTGCTA TTTTCTGGTA GTTCTTCGGA GTGTGTTGCT TTAATTATTA

 3721 AATTTATATA ATCAATGAAT TTGGGATCGT CGGTTTTGTA CAATATGTTG CCGGCATAGT

 3781 ACGCAGCTTC TTCTAGTTCA ATTACACCAT TTTTTAGCAG CACCGGATTA ACATAACTTT

 3841 CCAAAATGTT GTACGAACCG TTAAACAAAA ACAGTTCACC TCCCTTTTCT ATACTATTGT

 3901 CTGCGAGCAG TTGTTTGTTG TTAAAAATAA CAGCCATTGT AATGAGACGC ACAAACTAAT

 3961 ATCACAAACT GGAAATGTCT ATCAATATAT AGTTGCTGAT ATCATGGAGA TAATTAAAAT

 4021 GATAACCATC TCGCAAATAA ATAAGTATTT TACTGTTTTC GTAACAGTTT TGTAATAAAA

 4081 AAACCTATAA ATATTCCGGA TTATTCATAC CGTCCCACCA TCGGGCGCGG ATCCCGGGTA

 4141 CCTTCTAGAA TTCCGGAGCG GCCGCTGCAG ATCTGATCCT TTCCTGGGAC CCGGCAAGAA

 4201 CCAAAAACTC ACTCTCTTCA AGGAAATCCG TAATGTTAAA CCCGACACGA TGAAGCTTGT

 4261 CGTTGGATGG AAAGGAAAAG AGTTCTACAG GGAAACTTGG ACCCGCTTCA TGGAAGACAG

 4321 CTTCCCCATT GTTAACGACC AAGAAGTGAT GGATGTTTTC CTTGTTGTCA ACATGCGTCC

 4381 CACTAGACCC AACCGTTGTT ACAAATTCCT GGCCCAACAC GCTCTGCGTT GCGACCCCGA

 4441 CTATGTACCT CATGACGTGA TTAGGATCGT CGAGCCTTCA TGGGTGGGCA GCAACAACGA

 4501 GTACCGCATC AGCCTGGCTA AGAAGGGCGG CGGCTGCCCA ATAATGAACC TTCACTCTGA

 4561 GTACACCAAC TCGTTCGAAC AGTTCATCGA TCGTGTCATC TGGGAGAACT TCTACAAGCC

 4621 CATCGTTTAC ATCGGTACCG ACTCTGCTGA AGAGGAGGAA ATTCTCCTTG AAGTTTCCCT

 4681 GGTGTTCAAA GTAAAGGAGT TTGCACCAGA CGCACCTCTG TTCACTGGTC CGGCGTATTA

 4741 AAACACGATA CATTGTTATT AGTACATTTA TTAAGCGCTA GATTCTGTGC GTTGTTGATT

 4801 TACAGACAAT TGTTGTACGT ATTTTAATAA TTCATTAAAT TTATAATCTT TAGGGTGGTA

 4861 TGTTAGAGCG AAAATCAAAT GATTTTCAGC GTCTTTATAT CTGAATTTAA ATATTAAATC

 4921 CTCAATAGAT TTGTAAAATA GGTTTCGATT AGTTTCAAAC AAGGGTTGTT TTTCCGAACC

 4981 GATGGCTGGA CTATCTAATG GATTTTCGCT CAACGCCACA AAACTTGCCA AATCTTGTAG

 5041 CAGCAATCTA GCTTTGTCGA TATTCGTTTG TGTTTTGTTT TGTAATAAAG GTTCGACGTC

 5101 GTTCAAAATA TTATGCGCTT TTGTATTTCT TTCATCACTG TCGTTAGTGT ACAATTGACT

 5161 CGACGTAAAC ACGTTAAATA AAGCTTGGAC ATATTTAACA TCGGGCGTGT TAGCTTTATT

 5221 AGGCCGATTA TCGTCGTCGT CCCAACCCTC GTCGTTAGAA GTTGCTTCCG AAGACGATTT

 5281 TGCCATAGCC ACACGACGCC TATTAATTGT GTCGGCTAAC ACGTCCGCGA TCAAATTTGT

 5341 AGTTGAGCTT TTTGGAATTA TTTCTGATTG CGGGCGTTTT TGGGCGGGTT TCAATCTAAC

 5401 TGTGCCCGAT TTTAATTCAG ACAACACGTT AGAAAGCGAT GGTGCAGGCG GTGGTAACAT

 5461 TTCAGACGGC AAATCTACTA ATGGCGGCGG TGGTGGAGCT GATGATAAAT CTACCATCGG

 5521 TGGAGGCGCA GGCGGGGCTG GCGGCGGAGG CGGAGGCGGA GGTGGTGGCG GTGATGCAGA

 5581 CGGCGGTTTA GGCTCAAATG TCTCTTTAGG CAACACAGTC GGCACCTCAA CTATTGTACT

 5641 GGTTTCGGGC GCCGTTTTTG GTTTGACCGG TCTGAGACGA GTGCGATTTT TTTCGTTTCT

 5701 AATAGCTTCC AACAATTGTT GTCTGTCGTC TAAAGGTGCA GCGGGTTGAG GTTCCGTCGG

 5761 CATTGGTGGA GCGGGCGGCA ATTCAGACAT CGATGGTGGT GGTGGTGGTG GAGGCGCTGG

 5821 AATGTTAGGC ACGGGAGAAG GTGGTGGCGG CGGTGCCGCC GGTATAATTT GTTCTGGTTT

 5881 AGTTTGTTCG CGCACGATTG TGGGCACCGG CGCAGGCGCC GCTGGCTGCA CAACGGAAGG

 5941 TCGTCTGCTT CGAGGCAGCG CTTGGGGTGG TGGCAATTCA ATATTATAAT TGGAATACAA

 6001 ATCGTAAAAA TCTGCTATAA GCATTGTAAT TTCGCTATCG TTTACCGTGC CGATATTTAA

 6061 CAACCGCTCA ATGTAAGCAA TTGTATTGTA AAGAGATTGT CTCAAGCTCG CCGCACGCCG

 6121 ATAACAAGCC TTTTCATTTT TACTACAGCA TTGTAGTGGC GAGACACTTC GCTGTCGTCG

 6181 ACGTACATGT ATGCTTTGTT GTCAAAAACG TCGTTGGCAA GCTTTAAAAT ATTTAAAAGA

 6241 ACATCTCTGT TCAGCACCAC TGTGTTGTCG TAAATGTTGT TTTTGATAAT TTGCGCTTCC

 6301 GCAGTATCGA CACGTTCAAA AAATTGATGC GCATCAATTT TGTTGTTCCT ATTATTGAAT

 6361 AAATAAGATT GTACAGATTC ATATCTACGA TTCGTCATGG CCACCACAAA TGCTACGCTG

 6421 CAAACGCTGG TACAATTTTA CGAAAACTGC AAAAACGTCA AAACTCGGTA TAAAATAATC

 6481 AACGGGCGCT TTGGCAAAAT ATCTATTTTA TCGCACAAGC CCACTAGCAA ATTGTATTTG

 6541 CAGAAAACAA TTTCGGCGCA CAATTTTAAC GCTGACGAAA TAAAAGTTCA CCAGTTAATG

 6601 AGCGACCACC CAAATTTTAT AAAAATCTAT TTTAATCACG GTTCCATCAA CAACCAAGTG

 6661 ATCGTGATGG ACTACATTGA CTGTCCCGAT TTATTTGAAA CACTACAAAT TAAAGGCGAG

 6721 CTTTCGTACC AACTTGTTAG CAATATTATT AGACAGCTGT GTGAAGCGCT CAACGATTTG

 6781 CACAAGCACA ATTTCATACA CAACGACATA AAACTCGAAA ATGTCTTATA TTTCGAAGCA

 6841 CTTGATCGCG TGTATGTTTG CGATTACGGA TTGTGCAAAC ACGAAAACTC ACTTAGCGTG

 6901 CACGACGGCA CGTTGGAGTA TTTTAGTCCG GAAAAAATTC GACACACAAC TATGCACGTT

 6961 TCGTTTGACT GGTACGCGGC GTGTTAACAT ACAAGTTGCT AACGTAATCA TGGTCATAGC

 7021 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA

 7081 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT

 7141 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC

 7201 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC

 7261 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT

 7321 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG

 7381 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG

 7441 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT

 7501 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA

 7561 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT

 7621 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC

 7681 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA

 7741 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG

 7801 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG

 7861 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT

 7921 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA

 7981 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC

 8041 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA

 8101 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA

 8161 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT

 8221 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT

 8281 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT

 8341 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT

 8401 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA

 8461 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG

 8521 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT

 8581 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG

 8641 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG

 8701 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC

 8761 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA

 8821 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC

 8881 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT

 8941 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG

 9001 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA

 9061 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA

 9121 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA

 9181 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC

 9241 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT

 9301 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG

 9361 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA

 9421 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC

 9481 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC

 9541 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC

 9601 AGTCACGACG TTGTAAAACG ACGGCCAGTG CC

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pVL1393其他昆虫表达载体：