北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    YCplac22
YCplac22

YCplac22

YCplac22

YCplac22



 

编号

载体名称

北京华越洋VECT76336

YCplac22

 

YCplac22载体基本信息:

质粒类型:

酵母表达载体

低拷贝/高拷贝:

高拷贝

启动子:

lac

载体大小:

4854 bp

载体抗性:

Ampicillin (氨苄青霉素)

筛选标记:

TRP1


YCplac22载体图谱:

YCplac22载体序列:


GCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTT
CCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGG
CTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAA
CAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAG
CTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGC
CTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAAC
AGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTC
ACACCGCATATATCGCTGGGCCATTCTCATGAAGAATATCTTGAATTTATTGTCATATTACTAGTTGGTG
TGGAAGTCCATATATCGGTGATCAATATAGTGGTTGACATGCTGGCTAGTCAACATTGAGCCTTTTGATC
ATGCAAATATATTACGGTATTTTACAATCAAATATCAAACTTAACTATTGACTTTATAACTTATTTAGGT
GGTAACATTCTTATAAAAAAGAAAAAAATTACTGCAAAACAGTACTAGCTTTTAACTTGTATCCTAGGTT
ATCTATGCTGTCTCACCATAGAGAATATTACCTATTTCAGAATGTATGTCCATGATTCGCCGGGTAAATA
CATATAATACACAAATCTGGCTTAATAAAGTCTATAATATATCTCATAAAGAAGTGCTAAATTGGCTAGT
GCTATATATTTTTAAGAAAATTTCTTTTGACTAAGTCCATATCGACTTTGTAAAAGTTCACTTTAGCATA
CATATATTACACGAGCCAGAAATTGTAACTTTTGCCTAAAATCACAAATTGCAAAATTTAATTGCTTGCA
AAAGGTCACATGCTTATAATCAACTTTTTTAAAAATTTAAAATACTTTTTTATTTTTTATTTTTAAACAT
AAATGAAATAATTTATTTATTGTTTATGATTACCGAAACATAAAACCTGCTCAAGAAAAAGAAACTGTTT
TGTCCTTGGAAAAAAAGCACTACCTAGGAGCGGCCAAAATGCCGAGGCTTTCATAGCTTAAACTCTTTAC
AGAAAATAGGCATTATAGATCAGTTCGAGTTTTCTTATTCTTCCTTCCGGTTTTATCGTCACAGTTTTAC
AGTAAATAAGTATCACCTCTTAGAGTTCGATGATAAGCTGTCAAACATGAGAATTAATTCCACATGTTAA
AATAGTGAAGGAGCATGTTCGGCACACAGTGGACCGAACGTGGGGTAAGTGCACTAGGGTCCGGTTAAAC
GGATCTCGCATTGATGAGGCAACGCTAATTATCAACATATAGATTGTTATCTATCTGCATGAACACGAAA
TCTTTACTTGACGACTTGAGGCTGATGGTGTTTATGCAAAGAAACCACTGTGTTTAATATGTGTCACTGT
TTGATATTACTGTCAGCGTAGAAGATAATAGTAAAAGCGGTTAATAAGTGTATTTGAGATAAGTGTGATA
AAGTTTTTACAGCGAAAAGACGATAAATACAAGAAAATGATTACGAGGATACGGAGAGAGGTATGTACAT
GTGTATTTATATACTAAGCTGCCGGCGGTTGTTTGCAAGACCGAGAAAAGGCTAGCAAGAATCGGGTCAT
TGTAGCGTATGCGCCTGTGAACATTCTCTTCAACAAGTTTGATTCCATTGCGGTGAAATGGTAAAAGTCA
ACCCCCTGCGATGTATATTTTCCTGTACAATCAATCAAAAAGCCAAATGATTTAGCATTATCTTTACATC
TTGTTATTTTACAGATTTTATGTTTAGATCTTTTATGCTTGCTTTTCAAAAGGCTTGCAGGCAAGTGCAC
AAACAATACTTAAATAAATACTACTCAGTAATAACCTATTTCTTAGCATTTTTGACGAAATTTGCTATTT
TGTTAGAGTCTTTTACACCATTTGTCTCCACACCTCCGCTTACATCAACACCAATAACGCCATTTAATCT
AAGCGCATCACCAACATTTTCTGGCGTCAGTCCACCAGCTAACATAAAATGTAAGCTCTCGGGGCTCTCT
TGCCTTCCAACCCAGTCAGAAATCGAGTTCCAATCCAAAAGTTCACCTGTCCCACCTGCTTCTGAATCAA
ACAAGGGAATAAACGAATGAGGTTTCTGTGAAGCTGCACTGAGTAGTATGTTGCAGTCTTTTGGAAATAC
GAGTCTTTTAATAACTGGCAAACCGAGGAACTCTTGGTATTCTTGCCACGACTCATCTCCATGCAGTTGG
ACGATATCAATGCCGTAATCATTGACCAGAGCCAAAACATCCTCCTTAGGTTGATTACGAAACACGCCAA
CCAAGTATTTCGGAGTGCCTGAACTATTTTTATATGCTTTTACAAGACTTGAAATTTTCCTTGCAATAAC
CGGGTCAATTGTTCTCTTTCTATTGGGCACACATATAATACCCAGCAAGTCAGCATCGGAATCTAGTGCA
CATTCTGCGGCCTCTGTGCTCTGCAAGCCGCAAACTTTCACCAATGGACCAGAACTACCTGTGAAATTAA
TAACAGACATACTCCAAGCTGCCTTTGTGTGCTTAATCACGTATACTCACGTGCTCAATAGTCACCAATG
CCCTCCCTCTTGGCCCTCTCCTTTTCTTTTTTCGACCGAATTAATTCTTGAAGACGAAAGGGCCTCGTGA
TACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGG
AAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAA
TAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCC
TTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGA
TGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAG
AGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTAT
CCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTA
CTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACC
ATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTT
TGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAA
CGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTA
CTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGC
GCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTAT
CATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCA
ACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAG
ACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAA
GATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCC
GTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAA
AACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGG
CTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAAC
TCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGT
CGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGG
TTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGA
GAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAG
AGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTG
ACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCC
TTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTG
TGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGA
GTCAGTGAGCGAGGAAGCGGAAGA

 

YCplac22载体相关的酵母表达载体:

pKLAC1YIplac211
pHiSi-1YEplac181
pHISiYCplac22
pGBKlampYX212-VHB
pGBK-53pYES2-flag
pGAD424pYES2/NT C
pGAD53mpYES2/NT B
pFLD-cat Z+pYES2/NT A
pFLD-cat A+pYES2/CT/α-Factor
pFLD1 Z+pYADE4
pESP-3pUG35
pESP-2pP-αSUMO3
pESP-1pPICZalpha GB
pDR196pPICZalpha GA
pDNR1pPICZalpha FC
pBLADE-SXpPICZalpha E
pACT2 ADpMETα C
p426 GALpMETα B
P416 GPDpMETα A
pPIC6αApYD1
pRS406p53his
pRS416pCL1
pSEP1pSos
pHisSi-1pGBKT7-53
pMyrpFLDα
pGBKT7-LampADH2
pDisplaypYIP5
pPinkα-HCpDR195
pPIC3.5KpYES2
pAUR123pPIC ZαD
pPICZ CYcplac33
pHIC-PIpYIP211
pRS426galpYES2-kan
p416GFDpPICZα B
pYCP211pESC-Leu
pRS426pYES2-NTC
pESC-TRPpMETαB
pYES3/CTpMET A
pMETαCpHIL-S1
pMET BpPICZα C
pHIL-D2pPIC3.5
pGAPZα ApUG66
pAO815pDEST32
pGAPZ CpYC2/NT A
pYC2/NT CpYES-DEST52
pYES2/CTpPIC6αC
pPIC6 ApRS403
pGAG424pSos-MAFB
pGADT7-TpBridge
pHis2pACT2-AD
pGBKT7pFLD/CAT
pFLDpYES2-EGFP
Ycp22lac-EGFPpPIC9k-His
SUMO proteasepPICZ A
pGADT7LA503
BFD15pPIC ZαFC
pPIC ZαGBpYEPlac112
pYEPlac195pYRP7
pYX212pFA6a-FGP(S65T)-kanMX6
pRS41HpESC-URA
pRS316pYES6/CT
pESC-HispYES2-NTB
pYES2-NTApMET C
pMETαApPink-HC
pPink-LCpGAPZ B
pGAPZα CpPICZα A
pPICZ BpYC2/CT
pPIC9pRS405
pGAPZ ApRS414
pAD-GAL4-2.1pSEP2
pYC2/NT Bp53blue
pPIC6 CpRS415
pPIC6αBpSEP3
pRS413pBD-GAL4-Cam