北京华越洋生物
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首页    载体及质粒    pMETα B
pMETαA

pMETα B

pMETα B

pMETα B



 

编号

载体名称

北京华越洋VECT76329

pMETα B

 

pMETα B载体基本信息:

别名:

pMETalpha B

质粒类型:

酵母表达载体

表达水平:

高拷贝

诱导方法:

甲醇诱导

启动子:

AUG1

克隆方法:

多克隆位点,限制性内切酶

载体大小:

8049 bp

5' 测序引物及序列:

AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´;  alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´

3' 测序引物及序列:

AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´

载体标签:

C-V5, C-His,N-alpha分泌因子

载体抗性:

Ampicillin (氨苄青霉素)

筛选标记:

ADE2

备注:

含有alpha factor酵母分泌信号肽,利用甲醇诱导蛋白在

methanoloica酵母中分泌表达蛋白。

 

pMETα B载体图谱:

pMETα B载体序列:

 

GGCGCGCCTTAATTAAGGCCGGCCCCTGCAGGGGGCCCCGAGATGGTACATACTTAAAAGCTGCCATATT
GAGGAACTTCAAAGTTTTATCTGTTTTTAGAATTAAAAGACGATTGTTGTAACAAAACGTTGTGCCTACA
TAAACTCAAATTAATGGAAATAGCCTGTTTTGAAAAATACACCTTCTTAAGTACTGACAAAGTTTTGTTA
AATGACTATCGAACAAGCCATGAAATAGCACATTTCTGCCAGTCACTTTTAACACTTTCCTGCTTGCTGG
TTGACTCTCCTCATACAAACACCCAAAAGGGAAACTTTCAGTGTGGGGACACTTGACATCTCACATGCAC
CCCAGATTAATTTCCCCAGACGATGCGGAGACAAGACAAAACAACCCTTTGTCCTGCTCTTTTCTTTCTC
ACACCGCGTGGGTGTGTGCGCAGGCAGGCAGGCAGGCAGCGGGCTGCCTGCCATTTCTAATCGCTGCTCC
TCCCCCCTGGCTTCAAATAACAGCCTGCTGCTATCTGTGACCAGATTGGGACACCCCCCTCCCCTCCGAA
TGATCCATCACCTTTTGTCGTACTCCGACAATGATCCTTCCCTGTCATCTTCTGGCAATCAGCTCCTTCA
ATAATTAAATCAAATAAGCATAAATAGTAAAATCGCATACAAACGTCATGAAAAGTTTTATCTCTATGGC
CAACGGATAGTCTATCTGCTTAATTCCATCCACTTTGGGAACCGTTCTCTCTTTACCCCAGATTCTCAAA
GCTAATATCTGCCCCTTGTCTATTGTCCTTTCTCCGTGTACAAGCGGAGCTTTTGCCTCCCATCCTCTTG
CTTTGTTTCGGTTATTTTTTTTTTCTTTTGAAACTCTTGGTCAAATCAAATCAAACAAAACCAAACCTTC
TATTCCATCAGATCAACCTTGTTCAACATTCTATAAATCGATATAAATATAACCTTATCCCTCCCTTGTT
TTTTACCAATTAATCAATCTTCAAATTTCAAATATTTTCTACTTGCTTTATTACTCAGTATTAACATTTG
TTTAAACCAACTATAACTTTTAACTGGCTTTAGAAGTTTTATTTAACATCAGTTTCAATTTACATCTTTA
TTTATTAACGAAATCTTTACGAATTAACTCAATCAAAACTTTTACGAAAAAAAAATCTTACTATTAAGAG
CTCAAAATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAG
TCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGA
AGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACT
ACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCTCTCGAGAAAAGAGAGGCTGAAGCTGCAGGAA
TTCACGTGGCCCAGCCGGCCGTGGATCCACGCGTCGTCGACCCGCGGCGGCCGCCAGCTTACTAGTAGGT
AAGCCTATCCCTAACCCTCTCCTCGGTCTCGATTCTACGCGTACCGGTCATCATCACCATCACCATTGAT
CTAGTATACAATTCTAGGGCTGCCTGTTTGGATATTTTTATAATTTTTGAGAGTTTGCCAACTAATGTTT
TTCTCTTCTATGATATTTATCATGTAGTTGGATATCTTTTTTTCTTTGAAAAGTTTTTATTTAGTTCTTT
CTCTCTTTTTTATAATGTTTTCAGTTTCTTTCCTTTATATTGAAAATACGATGTTCTTTGTCTTTCTTTT
TTAAATCCTCAATTATAATGTGTACCGTTTGATCAACTTGTAAAATTGTTTGTAGTCCTGTAATCTTAAT
TGACGATTGGTTGATAGTTTACCCCAGTATATTGTGCGGCTGAGATATTACCCGATTCGGAAGATTGATT
TTTTCAACAGACATAAACCCATAGGAGATTTAGTACAGCGACATATGCTAATTGCCTGATAATGCTCACT
AACATTTTCTCCTTTTAATCTGGTATATGCATACAGGTATGTTGATATGGCCTTGTTGATCAAAGCTACG
AAAGTGTAGACTATTTCCCAAACAGCCCCCAACCACCACCTCACACGCGCACACTGCGAAGTGAAAGCGC
TCCCTTATTTTCTTCTCCGCTGGTTCAAAACAAAAAAGAATCTAATCTGTAGACACACGCCCCGTAAAAC
CCAATCTTCATCATCGTAATTTTCAGTGACGGACGATCGATCAACCATATAGTTTGTTAGTGACTGAGCT
TCAGTAGTTGACCTCTTGAAGAAATAACGTCTTGAACAACATACTTTATACCTACCAACATTCGCCATCA
ACAACAGTGTGACACTGACATAACTTTACATCCATTCATCGACAAAAAAATGTCTCTAGTTCGGATTCAT
GCTTATGGGTTAGCTATTTCGCCCAATGTGTCCATCTGACATTACTATTTTGCATTTTAATTTAATTAGA
ACTTGACTAGCGCACTACCAGCATATCATCTCATTTCCGTAAATACCAAATGTATTATATATTGAAAGCT
TTTGACCAGGTTATTATAAAAGAAACTTCATGCTCGAAAAAGATCATTTCGAAAAGTTGCCTAGTTTCAT
GAAATTTTAAAGCAGTTTATATAAATTTTACCTTTTGATGCGGAATTGACTTTTTCTTGAATAATACATA
ACTTTTCTTAAAAGAATCAAAGACAGATAAAATTTAAGAGATATTAAATATTAGTGAGAAGCCGAGAATT
TTGTAACACCAACATAACACTGACATCTTTAACAACTTTTAATTATGATACATTTCTTACGTCATGATTG
ATTATTACAGCTATGCTGACAAATGACTCTTGTTGCATGGCTACGAACCGGGTAATACTAAGTGATTGAC
TCTTGCTGACCTTTTATTAAGAACTAAATGGACAATATTATGGAGCATTTCATGTATAAATTGGTGCGTA
AAATCGTTGGATCTCTCTTCTAAGTACATCCTACTATAACAATCAAGAAAAACAAGAAAATCGGACAAAA
CAATCAAGTATGGATTCTAGAACAGTTGGTATATTAGGAGGGGGACAATTGGGACGTATGATTGTTGAGG
CAGCAAACAGGCTCAACATTAAGACGGTAATACTAGATGCTGAAAATTCTCCTGCCAAACAAATAAGCAA
CTCCAATGACCACGTTAATGGCTCCTTTTCCAATCCTCTTGATATCGAAAAACTAGCTGAAAAATGTGAT
GTGCTAACGATTGAGATTGAGCATGTTGATGTTCCTACACTAAAGAATCTTCAAGTAAAACATCCCAAAT
TAAAAATTTACCCTTCTCCAGAAACAATCAGATTGATACAAGACAAATATATTCAAAAAGAGCATTTAAT
CAAAAATGGTATAGCAGTTACCCAAAGTGTTCCTGTGGAACAAGCCAGTGAGACGTCCCTATTGAATGTT
GGAAGAGATTTGGGTTTTCCATTCGTCTTGAAGTCGAGGACTTTGGCATACGATGGAAGAGGTAACTTCG
TTGTAAAGAATAAGGAAATGATTCCGGAAGCTTTGGAAGTACTGAAGGATCGTCCTTTGTACGCCGAAAA
ATGGGCACCATTTACTAAAGAATTAGCAGTCATGATTGTGAGATCTGTTAACGGTTTAGTGTTTTCTTAC
CCAATTGTAGAGACTATCCACAAGGACAATATTTGTGACTTATGTTATGCGCCTGCTAGAGTTCCGGACT
CCGTTCAACTTAAGGCGAAGTTGTTGGCAGAAAATGCAATCAAATCTTTTCCCGGTTGTGGTATATTTGG
TGTGGAAATGTTCTATTTAGAAACAGGGGAATTGCTTATTAACGAAATTGCCCCAAGGCCTCACAACTCT
GGACATTATACCATTGATGCTTGCGTCACTTCTCAATTTGAAGCTCATTTGAGATCAATATTGGATTTGC
CAATGCCAAAGAATTTCACATCTTTCTCCACCATTACAACGAACGCCATTATGCTAAATGTTCTTGGAGA
CAAACATACAAAAGATAAAGAGCTAGAAACTTGCGAAAGAGCATTGGCGACTCCAGGTTCCTCAGTGTAC
TTATATGGAAAAGAGTCTAGACCTAACAGAAAAGTAGGTCACATAAATATTATTGCCTCCAGTATGGCGG
AATGTGAACAAAGGCTGAACTACATTACAGGTAGAACTGATATTCCAATCAAAATCTCTGTCGCTCAAAA
GTTGGACTTGGAAGCAATGGTCAAACCATTGGTTGGAATCATCATGGGATCAGACTCTGACTTGCCGGTA
ATGTCTGCCGCATGTGCGGTTTTAAAAGATTTTGGCGTTCCATTTGAAGTGACAATAGTCTCTGCTCATA
GAACTCCACATAGGATGTCAGCATATGCTATTTCCGCAAGCAAGCGTGGAATTAAAACAATTATCGCTGG
AGCTGGTGGGGCTGCTCACTTGCCAGGTATGGTGGCTGCAATGACACCACTTCCTGTCATCGGTGTGCCC
GTAAAAGGTTCTTGTCTAGATGGAGTAGATTCTTTACATTCAATTGTGCAAATGCCTAGAGGTGTTCCAG
TAGCTACCGTCGCTATTAATAATAGTACGAACGCTGCGCTGTTGGCTGTCAGACTGCTTGGCGCTTATGA
TTCAAGTTATACAACGAAAATGGAACAGTTTTTATTAAAGCAAGAAGAAGAAGTTCTTGTCAAAGCACAA
AAGTTAGAAACTGTCGGTTACGAAGCTTATCTAGAAAACAAGTAATATATAAGTTTATTGATATACTTGT
ACAGCAAATAATTATAAAATGATATACCTATTTTTTAGGCTTTGTTATGATTACATCAAATGTGGACTTC
ATACATAGAAATCAACGCTTACAGGTGTCCTTTTTTAAGAATTTCATACATAAGATCTACTAGAATCAAT
GTTAAACCTGTTATCATTAGAACAGCCATCTCCAGAAGGTTCTATCCAAGTGAAAACTTTGGTCTTCAAG
CCAAAGACTGCTAAAACACAAACCCCAGTCCCAATTATTGTTGTTGCTGAAACTTCTACTTCTACTCCGG
GTGGTGTTATAGCCAAAGCTTCGGGCACTAAAGAGCCAAGATTAGCAAACGATGAATTGACCAACCAATT
CTTTGGTTCTCCTGCTAAAGAGTTTTCTATTGCCAGCTTATCAAACGAACACAAAGACGGTAAAGTCAAG
ATCATTATTGATGCTGGGATCAAAGATGATGACTCGACTAATTACGTTATTTCCAACACTGTTGCTAAGT
TAGTTTTAACCGGTAAGCAATTATTAGGTTTCTTATCCAACTACGAACCAACCATCGTTGATTTTACTGC
TGAAGCTGCAACCGCTGCCCCAACTCCAGTTGTTAAGAAAGAACCTGCTGCATCTGGTAAGCTTGATGAT
GCCAAATTAATTGGTGTTACTGCTGACAAGGAAAAAGATTTCTCCAACTGGTACTCCCAAATTCTAATGA
AGGGTGAAATGTTAGACTACTACGATGTCTCTGGTTGTTACATTATGAGACCAGCTTCTTACACAATTTG
GGAAAAGATTCAATCTTGGTTCGATTCGAAGATTAAAGAAGATGGTGTTACTAATGCTTATTTCCCAATG
TTTGTCTCATCCAAGGTTTTAGAAAAGGAAAAGGACCACATCGATGGTTTCGCTCCTGAAGTTGCTTGGG
TTACCAGAGCTGGTAAATCTGAATTAGAAGAACCAATTGCTATTAGACCAACTTCTGAAACTGTTATGGG
TACCCTGCAGGGGCCGGCCTTAATTAAGGCGCGCCCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTT
CGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACG
CAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTT
TTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCG
ACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGC
CGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAG
GTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGAC
CGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAG
CAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCC
TAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAA
AGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGC
AGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTG
GAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTA
AATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCT
TAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGT
GTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGC
TCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAA
CTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAG
TTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTC
AGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCT
TCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCA
TAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTC
TGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATA
GCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCT
GTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGC
GTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTT
GAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATA
CATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCT
GTATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGAAATTGTAAGCGTTAAT
ATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCA
AAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCC
ACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAAGGGCGATGGCCCACTAC

 

pMETα B载体相关的酵母表达载体:

pKLAC1YIplac211
pHiSi-1YEplac181
pHISiYCplac22
pGBKlampYX212-VHB
pGBK-53pYES2-flag
pGAD424pYES2/NT C
pGAD53mpYES2/NT B
pFLD-cat Z+pYES2/NT A
pFLD-cat A+pYES2/CT/α-Factor
pFLD1 Z+pYADE4
pESP-3pUG35
pESP-2pP-αSUMO3
pESP-1pPICZalpha GB
pDR196pPICZalpha GA
pDNR1pPICZalpha FC
pBLADE-SXpPICZalpha E
pACT2 ADpMETα C
p426 GALpMETα B
P416 GPDpMETα A
pPIC6αApYD1
pRS406p53his
pRS416pCL1
pSEP1pSos
pHisSi-1pGBKT7-53
pMyrpFLDα
pGBKT7-LampADH2
pDisplaypYIP5
pPinkα-HCpDR195
pPIC3.5KpYES2
pAUR123pPIC ZαD
pPICZ CYcplac33
pHIC-PIpYIP211
pRS426galpYES2-kan
p416GFDpPICZα B
pYCP211pESC-Leu
pRS426pYES2-NTC
pESC-TRPpMETαB
pYES3/CTpMET A
pMETαCpHIL-S1
pMET BpPICZα C
pHIL-D2pPIC3.5
pGAPZα ApUG66
pAO815pDEST32
pGAPZ CpYC2/NT A
pYC2/NT CpYES-DEST52
pYES2/CTpPIC6αC
pPIC6 ApRS403
pGAG424pSos-MAFB
pGADT7-TpBridge
pHis2pACT2-AD
pGBKT7pFLD/CAT
pFLDpYES2-EGFP
Ycp22lac-EGFPpPIC9k-His
SUMO proteasepPICZ A
pGADT7LA503
BFD15pPIC ZαFC
pPIC ZαGBpYEPlac112
pYEPlac195pYRP7
pYX212pFA6a-FGP(S65T)-kanMX6
pRS41HpESC-URA
pRS316pYES6/CT
pESC-HispYES2-NTB
pYES2-NTApMET C
pMETαApPink-HC
pPink-LCpGAPZ B
pGAPZα CpPICZα A
pPICZ BpYC2/CT
pPIC9pRS405
pGAPZ ApRS414
pAD-GAL4-2.1pSEP2
pYC2/NT Bp53blue
pPIC6 CpRS415
pPIC6αBpSEP3
pRS413pBD-GAL4-Cam