pKLAC1
pKLAC1
编号 | 载体名称 |
北京华越洋VECT76322 | pKLAC1 |
pKLAC1载体基本信息：
质粒类型: | 乳酸酵母表达系统 |
启动子: | LAC4 |
克隆方法: | 多克隆位点，限制性内切酶 |
载体大小: | 9091 bp |
载体抗性: | Ampicillin (氨苄青霉素) |
pKLAC1载体图谱：
pKLAC1载体简介：
pKLAC1 is an expression vector capable both of replication in E. coli and stable integration into the genome of the yeast Kluyveromyces lactis (1). It is designed for high-level expression of recombinant protein in K. lactis using the K. lactis Protein Expression Kit (NEB #E1000).
pKLAC1载体序列：
AAGCTTGAAAAAAATGAAATTCTCTACTATATTAGCCGCATCTACTGCTTTAATTTCCGTTGTTATGGCT
GCTCCAGTTTCTACCGAAACTGACATCGACGATCTTCCAATATCGGTTCCAGAAGAAGCCTTGATTGGAT
TCATTGACTTAACCGGGGATGAAGTTTCCTTGTTGCCTGTTAATAACGGAACCCACACTGGTATTCTATT
CTTAAACACCACCATCGCTGAAGCTGCTTTCGCTGACAAGGATGATCTCGAGAAAAGAGAGGCTGAAGCT
AGAAGAGCTAGATCTCCTAGGGGTACCGTCGACGGCGCGCCTGCGGCCGCTTAATTAAGGCCTTGAATCG
AGAATTTATACTTAGATAAGTATGTACTTACAGGTATATTTCTATGAGATACTGATGTATACATGCATGA
TAATATTTAAACGGTTATTAGTGCCGATTGTCTTGTGCGATAATGACGTTCCTATCAAAGCAATACACTT
ACCACCTATTACATGGGCCAAGAAAATATTTTCGAACTTGTTTAGAATATTAGCACAGAGTATATGATGA
TATCCGTTAGATTATGCATGATTCATTCCTACAACTTTTTCGTAGCATAAGGATTAATTACTTGGATGCC
AATAAAAAAAAAAAACATCGAGAAAATTTCAGCATGCTCAGAAACAATTGCAGTGTATCAAAGTAAAAAA
AAGATTTTCACTACATGTTCCTTTTGAAGAAAGAAAATCATGGAACATTAGATTTACAAAAATTTAACCA
CCGCTGATTAACGATTAGACCGTTAAGCGCACAACAGGTTATTAGTACAGAGAAAGCATTCTGTGGTGTT
GCCCCGGACTTTCTTTTGCGACATAGGTAAATCGAATACCATCATACTATCTTTTCCAATGACTCCCTAA
AGAAAGACTCTTCTTCGATGTTGTATACGTTGGAGCATAGGGCAAGAATTGTGGCTTGAGATCATCCTTT
TGTTGTTTCCGGGTGTACAATATGGACTTCCTCTTTTCTGGCAACCAAACCCATACATCGGGATTCCTAT
AATACCTTCGTTGGTCTCCCTAACATGTAGGTGGCGGAGGGGAGATATACAATAGAACAGATACCAGACA
AGACATAATGGGCTAAACAAGACTACACCAATTACACTGCCTCATTGATGGTGGTACATAACGAACTAAT
ACTGTAGCCCTAGACTTGATAGCCATCATCATATCGAAGTTTCACTACCCTTTTTCCATTTGCCATCTAT
TGAAGTAATAATAGGCGCATGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCA
CCATATCCGCAATGACAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAA
CAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCA
CGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAA
AGTTTGCTGTCTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTC
TCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAAGGAA
TTCCGGATCCGCCACCATGCCTCAATCCTGGGAAGAACTGGCCGCTGATAAGCGCGCCCGCCTCGCAAAA
ACCATCCCTGATGAATGGAAAGTCCAGACGCTGCCTGCGGAAGACAGCGTTATTGATTTCCCAAAGAAAT
CGGGGATCCTTTCAGAGGCCGAACTGAAGATCACAGAGGCTTCCGCTGCGGATCTTGTGTCCAAGCTGGC
GGCCGGAGAGTTGACCTCGGTGGAAGTTACGCTAGCATTCTGTAAACGGGCAGCAATCGCCCAGCAGTTA
ACAAACTGCGCCCACGAGTTCTTCCCTGACGCCGCTCTCGCGCAGGCAAGGGAACTCGATGAATACTACG
CAAAGCACAAGAGACCCGTTGGTCCACTCCATGGCCTCCCCATCTCTCTCAAAGACCAGCTTCGAGTCAA
GGGCTACGAAACATCAATGGGCTACATCTCATGGCTAAACAAGTACGACGAAGGGGACTCGGTTCTGACA
ACCATGCTCCGCAAAGCCGGTGCCGTCTTCTACGTCAAGACCTCTGTCCCGCAGACCCTGATGGTCTGCG
AGACAGTCAACAACATCATCGGGCGCACCGTCAACCCACGCAACAAGAACTGGTCGTGCGGCGGCAGTTC
TGGTGGTGAGGGTGCGATCGTTGGGATTCGTGGTGGCGTCATCGGTGTAGGAACGGATATCGGTGGCTCG
ATTCGAGTGCCGGCCGCGTTCAACTTCCTGTACGGTCTAAGGCCGAGTCATGGGCGGCTGCCGTATGCAA
AGATGGCGAACAGCATGGAGGGTCAGGAGACGGTGCACAGCGTTGTCGGGCCGATTACGCACTCTGTTGA
GGACCTCCGCCTCTTCACCAAATCCGTCCTCGGTCAGGAGCCATGGAAATACGACTCCAAGGTCATCCCC
ATGCCCTGGCGCCAGTCCGAGTCGGACATTATTGCCTCCAAGATCAAGAACGGCGGGCTCAATATCGGCT
ACTACAACTTCGACGGCAATGTCCTTCCACACCCTCCTATCCTGCGCGGCGTGGAAACTACCGTCGCCGC
ACTCGCCAAAGCCGGTCACACCGTGACCCCGTGGACGCCATACAAGCACGATTTCGGCCACGATCTCATC
TCCCATATCTACGCGGCTGACGGCAGCGCCGACGTAATGCGCGATATCAGTGCATCCGGCGAGCCGGCGA
TTCCAAATATCAAAGACCTACTGAACCCGAACATCAAAGCTGTTAACATGAACGAGCTCTGGGACACGCA
TCTCCAGAAGTGGAATTACCAGATGGAGTACCTTGAGAAATGGCGGGAGGCTGAAGAAAAGGCCGGGAAG
GAACTGGACGCCATCATCGCGCCGATTACGCCTACCGCTGCGGTACGGCATGACCAGTTCCGGTACTATG
GGTATGCCTCTGTGATCAACCTGCTGGATTTCACGAGCGTGGTTGTTCCGGTTACCTTTGCGGATAAGAA
CATCGATAAGAAGAATGAGAGTTTCAAGGCGGTTAGTGAGCTTGATGCCCTCGTGCAGGAAGAGTATGAT
CCGGAGGCGTACCATGGGGCACCGGTTGCAGTGCAGGTTATCGGACGGAGACTCAGTGAAGAGAGGACGT
TGGCGATTGCAGAGGAAGTGGGGAAGTTGCTGGGAAATGTGGTGACTCCATAGCCCGGGGGGGGCTCGAT
CCCCTCGCGAGTTGGTTCAGCTGCTGCCTGAGGCTGGACGACCTCGCGGAGTTCTACCGGCAGTGCAAAT
CCGTCGGCATCCAGGAAACCAGCAGCGGCTATCCGCGCATCCATGCCCCCGAACTGCAGGAGTGGGGAGG
CACGATGGCCGCTTTGGTCGATCTAGATTACGTGGAAGAAAGGTAGTAAAAGTAGTAGTATAAGTAGTAA
AAAGAGGTAAAAAGAGAAAACCGGCTACATACTAGAGAAGCACGTACACAAAAACTCATAGGCACTTCAT
CATACGACAGTTTCTTGATGCATTATAATAGTGTATTAGATATTTTCAGAAATATGCATAGAACCTCCTC
TTGCCTTTACTTTTTATACATAGAACATTGGCAGATTTACTTACACTACTTTGTTTCTACGCCATTTCTT
TTGTTTTCAACACTTAGACAAGTTGTTGAGAACCGGACTACTAAAAAGCAATGTTCCCACTGAAAATCAT
GTACCTGCAGGATAATAACCCCCTAATTCTGCATCGATCCAGTATGTTTTTTTTTCTCTACTCATTTTTA
CCTGAAGATAGAGCTTCTAAAACAAAAAAAATCAGTGATTACATGCATATTGTGTGTTCTAGTAACCAAA
GGAAAGGAACAGATAGATAAAATTCCGAGACTGTCAAATTAGGTTTTTTTCTTTTTTTTTGGCGGGAGTC
AGTGGGCCGAAATATGTTCTTGGCCTAGAACTTAATCTGGTTTGATCATGCCAATACTTGCCTGAGTGCC
CGACTTTTTGCCCACCCTCTTGCCTTCTGTCATCCTTCAAAACCCACCTGTTTTCCAGCCGTATCTTCGC
TCGCATCTACACATACTGTGCCATATCTTGTGTGTAGCCGGACGTGACTATGACCAAAAACAAACAAGGA
GAACTGTTCGCCGATTTGTAACACTCCTGCATCCATCCAAGTGGGTATGCGCTATGCAATGTTAAGCTAG
GTCAGGTCAGACCAGGTCCAAGGACAGCAACTTGACTGTATGCAACCTTTACCATCTTTGCACAGAACAT
ACTTGTAGCTAGCTAGTTACACTTATGGACCGAAAAGGCACCCCACCATGTCTGTCCGGCTTTAGAGTAC
GGCCGCAGACCGCTGATTTGCCTTGCCAAGCAGTAGTCACAATGCATCGCATGAGCACACGGGCACGGGC
ACGGGCACAGGAACCATTGGCAAAAATACCAGATACACTATACCGACGTATATCAAGCCCAAGTTTAAAA
TTCCTAAATTTCCGCGGCTACTTTTCAATTCCCTATAGTGAGTCGTATTAAATTCGTAATCATGTCATAG
CTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTA
AAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTC
GGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGG
CGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTC
ACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGG
CCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGAC
GAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTT
TCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTT
CGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATC
GTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAG
AGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACA
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATC
TCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT
TTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAA
TCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGC
GATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGC
TTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAA
TAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTAT
TAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCT
ACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGC
GAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAG
TAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCC
GTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGA
GTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCAT
TGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCC
ACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAA
GGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCA
ATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAAT
AAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGCGCCCTGTAGCGGCGCATTAA
GCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTT
CGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCT
TTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTA
GTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACT
CTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCG
ATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAA
CGCTTACAATTTCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCG
CTATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCC
AGTCACGACGTTGTAAAACGACGGCCAGTGCCAAGCTCCCGCGGGGATCGACTCATAAAATAGTAACCTT
CTAATGCGTATCTATTGACTACCAACCATTAGTGTGGTTGCAGAAGGCGGAATTCTCCCTTCTTCGAATT
CAGCTTGCTTTTTCATTTTTTATTTTCCATTTTTCAGTTTTTGTTTGTGTCGAATTTAGCCAGTTGCTTC
TCCAAGATGAAAAAAACCCCTGCGCAGTTTCTGTGCTGCAAGATCCTAATCGACTTTTCCACCCCCCACA
AAAGTAAATGTTCTTTTGTTACATTCGCGTGGGTAGCTAGCTCCCCGAATCTTCAAAGGACTTAGGGACT
GCACTACATCAGAGTGTGTTCACCTGGTTTGCTGCCTGGTTTGAAAGAAAAGAGCAGGGAACTCGCGGGT
TCCCGGCGAATAATCATGCGATAGTCCTTTGGCCTTCCAAGTCGCATGTAGAGTAGACAACAGACAGGGA
GGGCAGGAAGGATCTTTCACTGAGATCCTGTATCTTGTTGGGTAAGTCGGATGAAAGGGGAATCGTATGA
GATTGGAGAGGATGCGGAAGAGGTAACGCCTTTTGTTAACTTGTTTAATTATTATGGGGCAGGCGAGAGG
GGGAGGAATGTATGTGTGTGAGGCGGGCGAGACGGAGCCATCCAGGCCAGGTAGAAATAGAGAAAGCCGA
ATGTTAGACAATATGGCAGCGTAGTAGAGTAGGTAGGTAGGCAAGTACTGCTAGCAAAGAGGAGAAGGGT
AAGCTCACTCTTCGCATTCCACACCGTTAGTGTGTCAGTTTGAACAAAAAAACAATCATCATACCAATTG
ATGGACTGTGGACTGGCTTTTGGAACGGCTTTTCGGACTGCGATTATTCGTGAGGAATCAAGGTAGGAAT
TTGGTCATATTTACGGACAACAGTGGGTGATTCCCATATGGAGTAGGAAAACGAGATCATGGTATCCTCA
GATATGTTGCGGAATTCTGTTCACCGCAAAGTTCAGGGTGCTCTGGTGGGTTTCGGTTGGTCTTTGCTTT
GCTTCTCCCTTGTCTTGCATGTTAATAATAGCCTAGCCTGTGAGCCGAAACTTAGGGTAGGCTTAGTGTT
GGAACGTACATATGTATCACGTTGACTTGGTTTAACCAGGCGACCTGGTAGCCAGCCATACCCACACACG
TTTTTTGTATCTTCAGTATAGTTGTGAAAAGTGTAGCGGAAATTTGTGGTCCGAGCAACAGCGTCTTTTT
CTAGTAGTGCGGTCGGTTACTTGGTTGACATTGGTATTTGGACTTTGTTGCTACACCATTCACTACTTGA
AGTCGAGTGTGAAGGGTATGATTTCTAGTGGTGAACACCTTTAGTTACGTAATGTTTTCATTGCTGTTTT
ACTTGAGATTTCGATTGAGAAAAAGGTATTTAATAGCTCGAATCAATGTGAGAACAGAGAGAAGATGTTC
TTCCCTAACTCGAAAGGTATATGAGGCTTGTGTTTCTTAGGAGAATTATTATTCTTTTGTTATGTTGCGC
TTGTAGTTGGAAAAGGTGAAGAGACAAAAGCTGGAATTGTGAGCGGATAACAAGCTCAACACTTGAAATT
TAGGAAAGAGCAGAATTTGGCAAAAAAAATAAAAAAAAAATAAACACACATACTCATCGAG
pKLAC1载体相关的酵母表达载体：
