pSFV1
pSFV1
编号 | 载体名称 |
北京华越洋VECT76056 | pSFV1 |
pSFV1载体基本信息：
质粒类型: | 哺乳动物表达载体 |
载体大小: | 11033 bp |
pSFV1载体图谱：
pSFV1载体简介：
The pSFV1 Eukaryotic Expression Vector is a novel DNA expression system based on the Semliki Forest Virus (SFV) replicon which has a broad host range and a high level of expression efficiency (1). In contrast to other protein expression systems, the SFV expression system has several advantages, including direct cloning into the vector and proper glycosylation of the recombinant protein. The SFV expression system is easy to use. The DNA of interest is cloned into the pSFV1 plasmid vector that serves as a template for in vitro synthesis of recombinant RNA, and the RNA is then transfected into tissue culture cells with DMRIE-C Reagent, Lipofectin® Reagent or by electroporation. The recombinant RNA in the cell drives its own replication and capping, resulting in production of large amounts of heterologous protein while inhibiting host protein synthesis. High transfection efficiencies and expression of heterologous proteins have been achieved by transfection of pSFV1 in vitro transcribed RNA into CHO-K1 and BHK-21 cells (2). The plasmid pSFV3- lacZ contains the E. coli #-galactosidase gene, lacZ, and is included as a control plasmid to optimize transfection conditions.
pSFV1载体序列：
GATGGCGGATGTGTGACATACACGACGCCAAAAGATTTTGTTCCAGCTCCTGCCACCTCCGCTACGCGAG
AGATTAACCACCCACGATGGCCGCCAAAGTGCATGTTGATATTGAGGCTGACAGCCCATTCATCAAGTCT
TTGCAGAAGGCATTTCCGTCGTTCGAGGTGGAGTCATTGCAGGTCACACCAAATGACCATGCAAATGCCA
GAGCATTTTCGCACCTGGCTACCAAATTGATCGAGCAGGAGACTGACAAAGACACACTCATCTTGGATAT
CGGCAGTGCGCCTTCCAGGAGAATGATGTCTACGCACAAATACCACTGCGTATGCCCTATGCGCAGCGCA
GAAGACCCCGAAAGGCTCGATAGCTACGCAAAGAAACTGGCAGCGGCCTCCGGGAAGGTGCTGGATAGAG
AGATCGCAGGAAAAATCACCGACCTGCAGACCGTCATGGCTACGCCAGACGCTGAATCTCCTACCTTTTG
CCTGCATACAGACGTCACGTGTCGTACGGCAGCCGAAGTGGCCGTATACCAGGACGTGTATGCTGTACAT
GCACCAACATCGCTGTACCATCAGGCGATGAAAGGTGTCAGAACGGCGTATTGGATTGGGTTTGACACCA
CCCCGTTTATGTTTGACGCGCTAGCAGGCGCGTATCCAACCTACGCCACAAACTGGGCCGACGAGCAGGT
GTTACAGGCCAGGAACATAGGACTGTGTGCAGCATCCTTGACTGAGGGAAGACTCGGCAAACTGTCCATT
CTCCGCAAGAAGCAATTGAAACCTTGCGACACAGTCATGTTCTCGGTAGGATCTACATTGTACACTGAGA
GCAGAAAGCTACTGAGGAGCTGGCACTTACCCTCCGTATTCCACCTGAAAGGTAAACAATCCTTTACCTG
TAGGTGCGATACCATCGTATCATGTGAAGGGTACGTAGTTAAGAAAATCACTATGTGCCCCGGCCTGTAC
GGTAAAACGGTAGGGTACGCCGTGACGTATCACGCGGAGGGATTCCTAGTGTGCAAGACCACAGACACTG
TCAAAGGAGAAAGAGTCTCATTCCCTGTATGCACCTACGTCCCCTCAACCATCTGTGATCAAATGACTGG
CATACTAGCGACCGACGTCACACCGGAGGACGCACAGAAGTTGTTAGTGGGATTGAATCAGAGGATAGTT
GTGAACGGAAGAACACAGCGAAACACTAACACGATGAAGAACTATCTGCTTCCGATTGTGGCCGTCGCAT
TTAGCAAGTGGGCGAGGGAATACAAGGCAGACCTTGATGATGAAAAACCTCTGGGTGTCCGAGAGAGGTC
ACTTACTTGCTGCTGCTTGTGGGCATTTAAAACGAGGAAGATGCACACCATGTACAAGAAACCAGACACC
CAGACAATAGTGAAGGTGCCTTCAGAGTTTAACTCGTTCGTCATCCCGAGCCTATGGTCTACAGGCCTCG
CAATCCCAGTCAGATCACGCATTAAGATGCTTTTGGCCAAGAAGACCAAGCGAGAGTTAATACCTGTTCT
CGACGCGTCGTCAGCCAGGGATGCTGAACAAGAGGAGAAGGAGAGGTTGGAGGCCGAGCTGACTAGAGAA
GCCTTACCACCCCTCGTCCCCATCGCGCCGGCGGAGACGGGAGTCGTCGACGTCGACGTTGAAGAACTAG
AGTATCACGCAGGTGCAGGGGTCGTGGAAACACCTCGCAGCGCGTTGAAAGTCACCGCACAGCCGAACGA
CGTACTACTAGGAAATTACGTAGTTCTGTCCCCGCAGACCGTGCTCAAGAGCTCCAAGTTGGCCCCCGTG
CACCCTCTAGCAGAGCAGGTGAAAATAATAACACATAACGGGAGGGCCGGCGGTTACCAGGTCGACGGAT
ATGACGGCAGGGTCCTACTACCATGTGGATCGGCCATTCCGGTCCCTGAGTTTCAAGCTTTGAGCGAGAG
CGCCACTATGGTGTACAACGAAAGGGAGTTCGTCAACAGGAAACTATACCATATTGCCGTTCACGGACCG
TCGCTGAACACCGACGAGGAGAACTACGAGAAAGTCAGAGCTGAAAGAACTGACGCCGAGTACGTGTTCG
ACGTAGATAAAAAATGCTGCGTCAAGAGAGAGGAAGCGTCGGGTTTGGTGTTGGTGGGAGAGCTAACCAA
CCCCCCGTTCCATGAATTCGCCTACGAAGGGCTGAAGATCAGGCCGTCGGCACCATATAAGACTACAGTA
GTAGGAGTCTTTGGGGTTCCGGGATCAGGCAAGTCTGCTATTATTAAGAGCCTCGTGACCAAACACGATC
TGGTCACCAGCGGCAAGAAGGAGAACTGCCAGGAAATAGTTAACGACGTGAAGAAGCACCGCGGGAAGGG
GACAAGTAGGGAAAACAGTGACTCCATCCTGCTAAACGGGTGTCGTCGTGCCGTGGACATCCTATATGTG
GACGAGGCTTTCGCTTGCCATTCCGGTACTCTGCTGGCCCTAATTGCTCTTGTTAAACCTCGGAGCAAAG
TGGTGTTATGCGGAGACCCCAAGCAATGCGGATTCTTCAATATGATGCAGCTTAAGGTGAACTTCAACCA
CAACATCTGCACTGAAGTATGTCATAAAAGTATATCCAGACGTTGCACGCGTCCAGTCACGGCCATCGTG
TCTACGTTGCACTACGGAGGCAAGATGCGCACGACCAACCCGTGCAACAAACCCATAATCATAGACACCA
CAGGACAGACCAAGCCCAAGCCAGGAGACATCGTGTTAACATGCTTCCGAGGCTGGGCAAAGCAGCTGCA
GTTGGACTACCGTGGACACGAAGTCATGACAGCAGCAGCATCTCAGGGCCTCACCCGCAAAGGGGTATAC
GCCGTAAGGCAGAAGGTGAATGAAAATCCCTTGTATGCCCCTGCGTCGGAGCACGTGAATGTACTGCTGA
CGCGCACTGAGGATAGGCTGGTGTGGAAAACGCTGGCCGGCGATCCCTGGATTAAGGTCCTATCAAACAT
TCCACAGGGTAACTTTACGGCCACATTGGAAGAATGGCAAGAAGAACACGACAAAATAATGAAGGTGATT
GAAGGACCGGCTGCGCCTGTGGACGCGTTCCAGAACAAAGCGAACGTGTGTTGGGCGAAAAGCCTGGTGC
CTGTCCTGGACACTGCCGGAATCAGATTGACAGCAGAGGAGTGGAGCACCATAATTACAGCATTTAAGGA
GGACAGAGCTTACTCTCCAGTGGTGGCCTTGAATGAAATTTGCACCAAGTACTATGGAGTTGACCTGGAC
AGTGGCCTGTTTTCTGCCCCGAAGGTGTCCCTGTATTACGAGAACAACCACTGGGATAACAGACCTGGTG
GAAGGATGTATGGATTCAATGCCGCAACAGCTGCCAGGCTGGAAGCTAGACATACCTTCCTGAAGGGGCA
GTGGCATACGGGCAAGCAGGCAGTTATCGCAGAAAGAAAAATCCAACCGCTTTCTGTGCTGGACAATGTA
ATTCCTATCAACCGCAGGCTGCCGCACGCCCTGGTGGCTGAGTACAAGACGGTTAAAGGCAGTAGGGTTG
AGTGGCTGGTCAATAAAGTAAGAGGGTACCACGTCCTGCTGGTGAGTGAGTACAACCTGGCTTTGCCTCG
ACGCAGGGTCACTTGGTTGTCACCGCTGAATGTCACAGGCGCCGATAGGTGCTACGACCTAAGTTTAGGA
CTGCCGGCTGACGCCGGCAGGTTCGACTTGGTCTTTGTGAACATTCACACGGAATTCAGAATCCACCACT
ACCAGCAGTGTGTCGACCACGCCATGAAGCTGCAGATGCTTGGGGGAGATGCGCTACGACTGCTAAAACC
CGGCGGCATCTTGATGAGAGCTTACGGATACGCCGATAAAATCAGCGAAGCCGTTGTTTCCTCCTTAAGC
AGAAAGTTCTCGTCTGCAAGAGTGTTGCGCCCGGATTGTGTCACCAGCAATACAGAAGTGTTCTTGCTGT
TCTCCAACTTTGACAACGGAAAGAGACCCTCTACGCTACACCAGATGAATACCAAGCTGAGTGCCGTGTA
TGCCGGAGAAGCCATGCACACGGCCGGGTGTGCACCATCCTACAGAGTTAAGAGAGCAGACATAGCCACG
TGCACAGAAGCGGCTGTGGTTAACGCAGCTAACGCCCGTGGAACTGTAGGGGATGGCGTATGCAGGGCCG
TGGCGAAGAAATGGCCGTCAGCCTTTAAGGGAGCAGCAACACCAGTGGGCACAATTAAAACAGTCATGTG
CGGCTCGTACCCCGTCATCCACGCTGTAGCGCCTAATTTCTCTGCCACGACTGAAGCGGAAGGGGACCGC
GAATTGGCCGCTGTCTACCGGGCAGTGGCCGCCGAAGTAAACAGACTGTCACTGAGCAGCGTAGCCATCC
CGCTGCTGTCCACAGGAGTGTTCAGCGGCGGAAGAGATAGGCTGCAGCAATCCCTCAACCATCTATTCAC
AGCAATGGACGCCACGGACGCTGACGTGACCATCTACTGCAGAGACAAAAGTTGGGAGAAGAAAATCCAG
GAAGCCATTGACATGAGGACGGCTGTGGAGTTGCTCAATGATGACGTGGAGCTGACCACAGACTTGGTGA
GAGTGCACCCGGACAGCAGCCTGGTGGGTCGTAAGGGCTACAGTACCACTGACGGGTCGCTGTACTCGTA
CTTTGAAGGTACGAAATTCAACCAGGCTGCTATTGATATGGCAGAGATACTGACGTTGTGGCCCAGACTG
CAAGAGGCAAACGAACAGATATGCCTATACGCGCTGGGCGAAACAATGGACAACATCAGATCCAAATGTC
CGGTGAACGATTCCGATTCATCAACACCTCCCAGGACAGTGCCCTGCCTGTGCCGCTACGCAATGACAGC
AGAACGGATCGCCCGCCTTAGGTCACACCAAGTTAAAAGCATGGTGGTTTGCTCATCTTTTCCCCTCCCG
AAATACCATGTAGATGGGGTGCAGAAGGTAAAGTGCGAGAAGGTTCTCCTGTTCGACCCGACGGTACCTT
CAGTGGTTAGTCCGCGGAAGTATGCCGCATCTACGACGGACCACTCAGATCGGTCGTTACGAGGGTTTGA
CTTGGACTGGACCACCGACTCGTCTTCCACTGCCAGCGATACCATGTCGCTACCCAGTTTGCAGTCGTGT
GACATCGACTCGATCTACGAGCCAATGGCTCCCATAGTAGTGACGGCTGACGTACACCCTGAACCCGCAG
GCATCGCGGACCTGGCGGCAGATGTGCACCCTGAACCCGCAGACCATGTGGACCTCGAGAACCCGATTCC
TCCACCGCGCCCGAAGAGAGCTGCATACCTTGCCTCCCGCGCGGCGGAGCGACCGGTGCCGGCGCCGAGA
AAGCCGACGCCTGCCCCAAGGACTGCGTTTAGGAACAAGCTGCCTTTGACGTTCGGCGACTTTGACGAGC
ACGAGGTCGATGCGTTGGCCTCCGGGATTACTTTCGGAGACTTCGACGACGTCCTGCGACTAGGCCGCGC
GGGTGCATATATTTTCTCCTCGGACACTGGCAGCGGACATTTACAACAAAAATCCGTTAGGCAGCACAAT
CTCCAGTGCGCACAACTGGATGCGGTCCAGGAGGAGAAAATGTACCCGCCAAAATTGGATACTGAGAGGG
AGAAGCTGTTGCTGCTGAAAATGCAGATGCACCCATCGGAGGCTAATAAGAGTCGATACCAGTCTCGCAA
AGTGGAGAACATGAAAGCCACGGTGGTGGACAGGCTCACATCGGGGGCCAGATTGTACACGGGAGCGGAC
GTAGGCCGCATACCAACATACGCGGTTCGGTACCCCCGCCCCGTGTACTCCCCTACCGTGATCGAAAGAT
TCTCAAGCCCCGATGTAGCAATCGCAGCGTGCAACGAATACCTATCCAGAAATTACCCAACAGTGGCGTC
GTACCAGATAACAGATGAATACGACGCATACTTGGACATGGTTGACGGGTCGGATAGTTGCTTGGACAGA
GCGACATTCTGCCCGGCGAAGCTCCGGTGCTACCCGAAACATCATGCGTACCACCAGCCGACTGTACGCA
GTGCCGTCCCGTCACCCTTTCAGAACACACTACAGAACGTGCTAGCGGCCGCCACCAAGAGAAACTGCAA
CGTCACGCAAATGCGAGAACTACCCACCATGGACTCGGCAGTGTTCAACGTGGAGTGCTTCAAGCGCTAT
GCCTGCTCCGGAGAATATTGGGAAGAATATGCTAAACAACCTATCCGGATAACCACTGAGAACATCACTA
CCTATGTGACCAAATTGAAAGGCCCGAAAGCTGCTGCCTTGTTCGCTAAGACCCACAACTTGGTTCCGCT
GCAGGAGGTTCCCATGGACAGATTCACGGTCGACATGAAACGAGATGTCAAAGTCACTCCAGGGACGAAA
CACACAGAGGAAAGACCCAAAGTCCAGGTAATTCAAGCAGCGGAGCCATTGGCGACCGCTTACCTGTGCG
GCATCCACAGGGAATTAGTAAGGAGACTAAATGCTGTGTTACGCCCTAACGTGCACACATTGTTTGATAT
GTCGGCCGAAGACTTTGACGCGATCATCGCCTCTCACTTCCACCCAGGAGACCCGGTTCTAGAGACGGAC
ATTGCATCATTCGACAAAAGCCAGGACGACTCCTTGGCTCTTACAGGTTTAATGATCCTCGAAGATCTAG
GGGTGGATCAGTACCTGCTGGACTTGATCGAGGCAGCCTTTGGGGAAATATCCAGCTGTCACCTACCAAC
TGGCACGCGCTTCAAGTTCGGAGCTATGATGAAATCGGGCATGTTTCTGACTTTGTTTATTAACACTGTT
TTGAACATCACCATAGCAAGCAGGGTACTGGAGCAGAGACTCACTGACTCCGCCTGTGCGGCCTTCATCG
GCGACGACAACATCGTTCACGGAGTGATCTCCGACAAGCTGATGGCGGAGAGGTGCGCGTCGTGGGTCAA
CATGGAGGTGAAGATCATTGACGCTGTCATGGGCGAAAAACCCCCATATTTTTGTGGGGGATTCATAGTT
TTTGACAGCGTCACACAGACCGCCTGCCGTGTTTCAGACCCACTTAAGCGCCTGTTCAAGTTGGGTAAGC
CGCTAACAGCTGAAGACAAGCAGGACGAAGACAGGCGACGAGCACTGAGTGACGAGGTTAGCAAGTGGTT
CCGGACAGGCTTGGGGGCCGAACTGGAGGTGGCACTAACATCTAGGTATGAGGTAGAGGGCTGCAAAAGT
ATCCTCATAGCCATGGCCACCTTGGCGAGGGACATTAAGGCGTTTAAGAAATTGAGAGGACCTGTTATAC
ACCTCTACGGCGGTCCTAGATTGGTGCGTTAATACACAGAATTCTGATTGGATCCCGGGTAATTAATTGA
ATTACATCCCTACGCAAACGTTTTACGGCCGCCGGTGGCGCCCGCGCCCGGCGGCCCGTCCTTGGCCGTT
GCAGGCCACTCCGGTGGCTCCCGTCGTCCCCGACTTCCAGGCCCAGCAGATGCAGCAACTCATCAGCGCC
GTAAATGCGCTGACAATGAGACAGAACGCAATTGCTCCTGCTAGGCCTCCCAAACCAAAGAAGAAGAAGA
CAACCAAACCAAAGCCGAAAACGCAGCCCAAGAAGATCAACGGAAAAACGCAGCAGCAAAAGAAGAAAGA
CAAGCAAGCCGACAAGAAGAAGAAGAAACCCGGAAAAAGAGAAAGAATGTGCATGAAGATTGAAAATGAC
TGTATCTTCGTATGCGGCTAGCCACAGTAACGTAGTGTTTCCAGACATGTCGGGCACCGCACTATCATGG
GTGCAGAAAATCTCGGGTGGTCTGGGGGCCTTCGCAATCGGCGCTATCCTGGTGCTGGTTGTGGTCACTT
GCATTGGGCTCCGCAGATAAGTTAGGGTAGGCAATGGCATTGATATAGCAAGAAAATTGAAAACAGAAAA
AGTTAGGGTAAGCAATGGCATATAACCATAACTGTATAACTTGTAACAAAGCGCAACAAGACCTGCGCAA
TTGGCCCCGTGGTCCGCCTCACGGAAACTCGGGGCAACTCATATTGACACATTAATTGGCAATAATTGGA
AGCTTACATAAGCTTAATTCGACGAATAATTGGATTTTTATTTTATTTTGCAATTGGTTTTTAATATTTC
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
ACTAGTCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTT
CCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGT
AATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCC
AGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAA
ATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAG
CTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGA
AGCGTGGCGCTTTCTCAATGCTCGCGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGG
GCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAA
CCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTA
GGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCT
GCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGC
TGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCT
TTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGAT
TATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATA
TGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTT
CGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCC
CCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGC
CGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGG
GAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGG
TGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATC
CCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCA
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTT
CTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCC
GGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCT
TCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCA
ACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGC
AAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGC
ATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGG
TTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTA
TAAAAATAGGCGTATCACGAGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACAC
ATGCAGCTCCCGGAGACGGTCACAGCTTCTGTCTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCG
CGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGT
GCACCATATCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTACTAGGTTGAGGCCGT
TGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCC
TGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTG
ATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGT
AGAGGATCTGGCTAGCGATGACCCTGCTGATTGGTTCGCTGACCATTTCCGGGGTGCGGAACGGCGTTAC
CAGAAACTCAGAAGGTTCGTCCAACCAAACCGACTCTGACGGCAGTTTACGAGAGAGATGATAGGGTCTG
CTTCAGTAAGCCAGATGCTACACAATTAGGCTTGTACATATTGTCGTTAGAACGCGGCTACAATTAATAC
ATAACCTTATGTATCATACACATACGATTTAGGTGACACTATA
pSFV1载体相关的哺乳动物表达载体：
