pBS185 CMV-Cre
pBS185 CMV-Cre
编号 | 载体名称 |
北京华越洋VECT76058 | pBS185 CMV-Cre |
pBS185 CMV-Cre载体基本信息：
质粒类型: | 哺乳动物表达载体,Cre/Lox系统 |
载体大小: | 6919 bp |
载体抗性: | Ampicillin |
pBS185 CMV-Cre载体图谱:
pBS185 CMV-Cre载体简介：
BS185 carries the wt cre gene under the control of the major immediate early promoter and enhancer of human cytomegalovirus (hCMV). This is a strong promoter that gives fairly generalized expression. However, it is not a strong promoter in embryonic stem (ES) cells. The construct also carries the mouse metallothionein MT-I region including the polyadenylation site and several introns. For higher level expression in general and for better expression in ES cells plasmid pBS513 is superior. The sequence shown includes cre and the metallothionein region.
pBS185 CMV-Cre载体序列：
AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTT
TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAG
GCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAA
ACAGCTATGACATGATTACGAATTCGAGCTCGGTACCCTCGACCAATTCCTGGCCTTCCTGCTGCTACAT
CTCAAATATCTTGATTACAGGCAGGACCCACGCTCTTTATCACTTTTTTTTCCATCTTTTTTTTTTTGGA
TATTTTCTTCGTTTATATTTCAAATGCTATCCCAAAAGTCCCCTATACCCCACCCCTACCCCCACCCCGC
TCCCCTACCCACTCACTCCCACTTCTTGGCCCTGGCTTTCCCCTGAACTGGGGCATATAGTTTGCTAGAC
CAAGGGTCCTCTCTTCCCAGTGATGGCCAACTAGGCCATCTTCTGCTACATACGAAGCTAGATACACGAG
TTCAGGGGGTACTGGTTAGTTCAGATTGCTGTTCCACCTATAGGGTTGCAGATCCCTTTAGCTCCTTGGG
TACTTTCTCTAGCTCCTCCATTGGGGGCCCTGTGTTCCATCCAATAGCTGACTGTGAGCATCCACTTCTG
TGTTTGCTGGGCACTGGCAAAGCCTCACAGGAGACAGCTTTATCAGGGTCCTTTCAGCAAAATCTTGATG
GCATATGCAATAGTGTCTGCATTTGGTGGCTGAATAGGAGATGGCCTGGTGACACAGTCACTCCTGTAAT
CCCAGGTACTTGGGAAGCTGAGGCAGGAGAATCAAAAACTCAAGGTCAGACTGGACTGCAGAATGAGTTC
TAGGACAAGGTAAGCAACTTGTGTCAAAACAGCCAAGAAAGCCCCTCTGGAATCTAGTTCAGTGTTAGTC
CCTGCCTAGCATACGAGACACCCTATGTTAATTTTCATACCCACCTCCTTATACCCAACAATGTGAGAGG
AAACCAGAATCCAAAGAGAATATAAATGATAAGATTTTCTGTGCTTCCAGGATGGGACATCAAGTCTTCC
AGGACCACCCTCCCTCCCTCCCTTCCCCTAACACAAGACCTGACCAGGCACACAGCTGTTTTCCATGAGG
AAGCTTATGGGATAGCTTGGCTTTACCCAAAGACATTACCCCTCTGTGAGGGCCAGAGGCGGGAGGTAAA
GGCACGAGGGGGTCCAAAACCAGATCCCCAGACTATCAAGTCACCCCAGTTTCTTATTGCCAAACCACAC
CAAGAAAACCAGAGTTAGACTCAAACAGGCTTTTATTATTCACGTACTCGGTAGAAAACGGGGGTTTAGT
AAACAGGGTGGAACTGTATAGGAAGACGCTGGGTTGGTCCGATACTATTTACACGTGGTGGCAGCGCTGT
TCGTCACATCAGGCACAGCACGTGCACTTGTCCGCGGCGCCTTTGCAGACACAGCCCTGGGCACATTTGG
AGCAGCCCACGGGACAGCAGGAGCAGCAGCCTAGAAAGGAGGAGGAAGAGGTCAGCCTGGCTGAAGACCT
CTGCTTCCTGTCCCCAACAGAGAAGACCTCGGTTTTTTTAAATAGTTCTCTGTATAGTTCTGTCCCCGAG
ACAATACAATGGCCTCCGGGAAGCAAAGGACATTTCTCAGAGCCAGTTTTGTAGGAGTTCCCCGCCCCTA
GCCTTAGCCGCCACCCAAGGTGTCCCAACTCACTCTTCTTGCAGGAGGTGCACTTGCAGTTCTTGCAGGC
GCAGGAGCTGGTGCAAGTGCAGGAGCCGCCTGGGGAGGAGAAAGAAGACAGCATGAGGGAGGCAGCATTA
CAGCAGTGGCCAACACCACGAGTCCCGGCTCAGTTCACTAAGTCCTCCTCGGAGCTGCAGGGAGCCTAGC
CCCACTTTTCTCCTCACAGGTTAAGTCAGGGATTATGTCTTTGAGTCCCAAGACATAAAGGTCCTTCACC
TCTTTCTTAATTCAAGCAATTCACAGTAAGTTTAGTAATGCCTGGGACTCAACCCAGATGTCCCCACTAA
CTGGGTGATCAGGAACCGGACATTTGCGCGTCTAAATCCTAGCCCCAACCCCGCCAAAGGACCCGTTCGA
CAATACTCGTAACTAATGAAGGCTTTTGCAAGAGCGGATTCTAGAAAGAACCGGAATGAATCGCCAACTA
AAGGTGCCTATTCCCGCCAAGCCTCTACAACTCGGGGATTCCGCTCTGCGGTCCCAACTTGGTATTCTAA
AGACCAAGGATCGGGAGTCTTACCGGTGGAGCAGGAGCAGTTGGGGTCCATTCCGAGATCCCTCGACAAT
TCCAACCTTACCCAAGAGTTCGCCAAACTCAGACATCACTTTAGCAAAACCGCGCCGTGCTTCTTCCTCG
GTGGCATTCATCACGAAATGTTCAGCACTACGCATACTTTTGACAGGAAACGCAACGGATATTGAGTCAA
TATCAGGCATTCTATCGCTCAGCTTTACAGTGACAATGACGGCTGGCGACTGAATATTAGTGCTTACAGA
CAGCACTACATATTTTCCGTCGATGTTGAAATCCTTTCTCATATGTCACCATAAATATCAAATAATTATA
GCAATCATTTACGCGTTAATGGCTAATCGCCATCTTCCAGCAGGCGCACCATTGCCCCTGTTTCACTATC
CAGGTTACGGATATAGTTCATGACAATATTTACATTGGTCCAGCCACCAGCTTGCATGATCTCCGGTATT
GAAACTCCAGCGCGGGCCATATCTCGCGCGGCTCCGACACGGGCACTGTGTCCAGACCAGGCCAGGTATC
TCTGACCAGAGTCATCCTTAGCGCCGTAAATCAATCGATGAGTTGCTTCAAAAATCCCTTCCAGGGCGCG
AGTTGATAGCTGGCTGGTGGCAGATGGCGCGGCAACACCATTTTTTCTGACCCGGCAAAACAGGTAGTTA
TTCGGATCATCAGCTACACCAGAGACGGAAATCCATCGCTCGACCAGTTTAGTTACCCCCAGGCTAAGTG
CCTTCTCTACACCTGCGGTGCTAACCAGCGTTTTCGTTCTGCCAATATGGATTAACATTCTCCCACCGTC
AGTACGTGAGATATCTTTAACCCTGATCCTGGCAATTTCGGCTATACGTAACAGGGTGTTATAAGCAATC
CCCAGAAATGCCAGATTACGTATATCCTGGCAGCGATCGCTATTTTCCATGAGTGAACGAACCTGGTCGA
AATCAGTGCGTTCGAACGCTAGAGCCTGTTTTGCACGTTCACCGGCATCAACGTTTTCTTTTCGGATCCG
CCGCATAACCAGTGAAACAGCATTGCTGTCACTTGGTCGTGGCAGCCCGGACCGACGATGAAGCATGTTT
AGCTGGCCCAAATGTTGCTGGATAGTTTTTACTGCCAGACCGCGCGCCTGAAGATATAGAAGATAATCGC
GAACATCTTCAGGTTCTGCGGGAAACCATTTCCGGTTATTCAACTTGCACCATGCCGCCCACGACCGGCA
AACGGACAGAAGCATTTTCCAGGTATGCTCAGAAAACGCCTGGCGATCCCTGAACATGTCCATCAGGTTC
TTGCGAACCTCATCACTCGTTGCATCGACCGGTAATGCAGGCAAATTTTGGTGTACGGTCAGTAAATTGG
ACATTTCACACTCAGATAATGGTTTTAAGTAAAGTGTACAGGATCGGCTCTGCCCCTCGAGGGGATCCTC
TAGAGTCGGTGTCTTCTATGGAGGTCAAAACAGCGTGGATGGCGTCTCCAGGCGATCTGACGGTTCACTA
AACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAATGGGGCGGAGTT
GTTACGACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGGGTGG
AGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGATGTACTGCCAAAACCGCATCACCA
TGGTAATAGCGATGACTAATACGTAGATGTACTGCCAAGTAGGAAAGTCCCATAAGGTCATGTACTGGGC
ATAATGCCAGGCGGGCCATTTACCGTCATTGACGTCAATAGGGGGCGTACTTGGCATATGATACACTTGA
TGTACTGCCAAGTGGGCAGTTTACCGTAAATACTCCACCCATTGACGTCAATGGAAAGTCCCTATTGGCG
TTACTATGGGAACATACGTCATTATTGACGTCAATGGGCGGGGGTCGTTGGGCGGTCAGCCAGGCGGGCC
ATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCCGTAATTGATTAC
TATTAATAACTAGTCAATAATCAATGTCAACATGGCGGTAATGTTGGACATGAGCCAATATAAATGTACA
TATTATGATATGGATACAACGTATGCAATGGGCCAAGCTTGGCACTGGCCGTCGTTTTACAACGTCGTGA
CTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAAT
AGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGC
GGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTC
TGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTG
CTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGT
CATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAAT
AATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTC
TAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAA
GGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGT
TTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTAC
ATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGA
GCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCG
CCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGC
ATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGA
CAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGA
TCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATG
GCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACT
GGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGA
TAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCC
CGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGA
TAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTT
AAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCT
TAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTT
TTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGA
TCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTT
CTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAA
TCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTT
ACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACC
TACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGG
ACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTG
GTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGG
GGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTG
CTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGA
TACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG
pBS185 CMV-Cre载体相关的哺乳动物表达载体：
