pZsYellow

pZsYellow

pZsYellow载体基本信息：

pZsYellow载体图谱：

pZsYellow载体简介：

pZsYellow is a pUC19-derived prokaryotic expression vector, which encodes a variant of wild-type Zoanthus sp. yellow fluorescent protein (ZsYellow) that has been engineered for brighter fluorescence. Wild-type ZsYellow cDNA was originally cloned from a Zoanthus species of coral, native to the Indian and Pacific oceans (1). A single amino acid substitution (Met-128 to Val) has been made to enhance the emission characteristics of ZsYellow (excitation maximum = 531 nm; emission maximum = 540 nm).

The ZsYellow gene was inserted in frame with the lacZ initiation codon from pUC19 so that ZsYellow is expressed from the lac promoter (Plac) in E. coli. The ZsYellow coding sequence is flanked by distinct multiple cloning sites (MCS) at the 5' and 3' ends so that the gene can be easily excised from pZsYellow and subcloned into other expression vectors. An upstream sequence—located just 5' to the ZsYellow gene—has been converted to a Kozak consensus translation initiation site (2) to increase the translation efficiency in eukaryotic expression systems. The pUC backbone of pZsYellow provides a high-copy-number origin of replication (pUC ori) and an ampicillin resistance gene (Ampr) for propagation and selection in E. coli.

pZsYellow载体应用：

pZsYellow Vector serves as a convenient source of the ZsYellow cDNA. The flanking MCS regions make it possible to excise the ZsYellow coding sequence and insert it into other prokaryotic or eukaryotic expression vectors. Alternatively, the ZsYellow coding sequence can be amplified by PCR.

pZsYellow载体序列：

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTT
TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAG
GCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAA
ACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGG
TCGCCACCATGGCTCATTCAAAGCACGGTCTAAAAGAAGAAATGACAATGAAATACCACATGGAAGGGTG
CGTCAACGGACATAAATTTGTGATCACGGGCGAAGGCATTGGATATCCGTTCAAAGGGAAACAGACTATT
AATCTGTGTGTGATCGAAGGGGGACCATTGCCATTTTCCGAAGACATATTGTCAGCTGGCTTTAAGTACG
GAGACAGGATTTTCACTGAATATCCTCAAGACATAGTAGACTATTTCAAGAACTCGTGTCCTGCTGGATA
TACATGGGGCAGGTCTTTTCTCTTTGAGGATGGAGCAGTCTGCATATGCAATGTAGATATAACAGTGAGT
GTCAAAGAAAACTGCATTTATCATAAGAGCATATTTAATGGAGTGAATTTTCCTGCTGATGGACCTGTGA
TGAAAAAGATGACAACTAACTGGGAAGCATCCTGCGAGAAGATCATGCCAGTACCTAAGCAGGGGATACT
GAAAGGGGATGTCTCCATGTACCTCCTTCTGAAGGATGGTGGGCGTTACCGGTGCCAGTTCGACACAGTT
TACAAAGCAAAGTCTGTGCCAAGTAAGATGCCGGAGTGGCACTTCATCCAGCATAAGCTCCTCCGTGAAG
ACCGCAGCGATGCTAAGAATCAGAAGTGGCAGCTGACAGAGCATGCTATTGCATTCCCTTCTGCCTTGGC
CTGAAGCGGCCGCGACTCTAGAATTCCAACTGAGCGCCGGTCGCTACCATTACCAACTTGTCTGGTGTCA
AAAATAATAGGCCTACTAGTCGGCCGTACGGGCCCTTTCGTCTCGCGCGTTTCGGTGATGACGGTGAAAA
CCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCC
CGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTG
TACTGAGAGTGCACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCG
GCCTTAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTC
AGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG
TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTC
AACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAAC
GCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGC
TATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCA
GAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA
TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGA
AGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT
GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAA
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAG
TTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGA
GCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC
ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA
AGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATT
TAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTC
CACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCT
GCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCT
TTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTA
GGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTG
CTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCG
GTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATAC
CTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCG
GCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGT
CGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAA
AACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTG
CGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCG
AACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG

pZsYellow载体其他相关的原核表达载体：