pRRLSIN.CPPT.PGK-GFP.WPRE
pRRLSIN.CPPT.PGK-GFP.WPRE
编号 | 载体名称 |
北京华越洋VECT75611 | pRRLSIN.CPPT.PGK-GFP.WPRE |
pRRLSIN.CPPT.PGK-GFP.WPRE载体基本信息：
启动子: | hPGK，T3，T7 |
复制子: | pUC ori,f1 ori,SV40 ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 病毒系列，慢病毒克隆载体 |
质粒大小: | 7384bp |
原核抗性: | Amp |
克隆菌株: | Stbl3 |
培养条件: | 37℃，有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | pEGFP-C-5:CATGGTCCTGCTGGAGTTCGTG |
3'测序引物: | 根据序列设计引物 |
pRRLSIN.CPPT.PGK-GFP.WPRE载体图谱：
pRRLSIN.CPPT.PGK-GFP.WPRE载体序列：
ORIGIN
1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
1921 ttttatcgat cacgagacta gcctcgagaa gcttgatatc gaattcccac ggggttgggg
1981 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
2041 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
2101 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
2161 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
2221 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
2281 cgatgggctg tggccaatag cggctgctca gcggggcgcg ccgagagcag cggccgggaa
2341 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
2401 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
2461 atcaccgacc tctctcccca gggggatcca ccggtcgcca ccatggtgag caagggcgag
2521 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
2581 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
2641 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
2701 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
2761 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
2821 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
2881 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
2941 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
3001 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
3061 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
3121 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
3181 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgtcgacaat
3241 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
3301 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
3361 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
3421 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
3481 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
3541 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
3601 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
3661 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
3721 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
3781 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct
3841 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
3901 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
3961 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
4021 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
4081 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
4141 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
4201 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
4261 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
4321 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag
4381 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
4441 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
4501 ttgcgtcgag acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
4561 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
4621 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
4681 caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc
4741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
4801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
4861 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
4921 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
4981 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
5041 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
5101 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
5161 tttacaattt cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
5221 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
5281 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
5341 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
5401 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
5461 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
5521 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
5581 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
5641 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
5701 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
5761 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
5821 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
5881 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
5941 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
6001 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
6061 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
6121 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
6181 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
6241 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
6301 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
6361 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
6421 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
6481 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
6541 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
6601 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
6661 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
6721 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
6781 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
6841 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
6901 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
6961 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
7021 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
7081 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
7141 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
7201 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
7261 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
7321 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
7381 tgca
//
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