北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pROKII
pROKII

pROKII

pROKII

pROKII



 

编号

载体名称

北京华越洋VECT75586

pROKII

 

pROKII载体基本信息:

启动子:

CaMV 35S

复制子:

ori

终止子:

NOS

质粒分类:

植物系列,蛋白过表达载体

质粒大小:

12880bp

原核抗性:

Kan

筛选标记:

Neo

克隆菌株:

DH5α

培养条件:

37℃,有氧 LB

5'测序引物:

35S:GACGCACAATCCCACTATCC

3'测序引物:

根据序列设计引物

 

pROKII载体图谱:


pROKII载体简介:

   An intron sequence of Poptr;CYCD1;1 from Populus trichocarpa was ligated into binary vector,pROKⅡ, to obtain the construct for gene silencing in plants. The resulting pRNAi was used to silence the exogenous fusion gene, GUS:GFP, in transgenic tobacco transformed with pCAMBIA-1303 vector. The specific sequence from GUS and GFP genes was cloned into pRNAi, respectively, and both of them reduced the expression of GUS:GFP gene. The results implied that the present pRNAi could be used to silence some gene of interest.

 

pROKII载体序列:

ORIGIN

        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc

       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc

      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat

      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc

      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag

      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc

      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc

      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg

      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc

      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata

      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg

      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata

      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta

      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg

      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt

      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag

      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt

     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta

     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt

     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg

     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga

     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg

     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac

     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc

     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg

     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc

     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc

     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg

     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg

     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg

     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg

     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc

     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa

     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg

     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa

     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc

     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc

     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg

     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc

     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct

     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc

     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga

     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc

     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc

     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc

     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa

     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg

     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt

     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg

     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg

     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg

     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg

     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca

     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc

     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc

     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca

     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga

     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg

     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg

     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg

     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga

     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag

     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct

     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat

     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg

     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg

     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc

     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc

     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg

     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata

     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc

     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg

     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag

     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta

     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca

     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt

     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg

     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt

     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc

     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag

     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt

     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca

     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag

     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg

     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa

     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat

     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc

     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat

     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt

     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca

     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat

     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat

     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg

     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc

     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt

     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca

     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga

     5821 ggatccccgg gtaccgagct cgaatttccc cgatcgttca aacatttggc aataaagttt

     5881 cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc tgttgaatta

     5941 cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat gggtttttat

     6001 gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat agcgcgcaaa

     6061 ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat tcactggccg

     6121 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag

     6181 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc

     6241 aacagttgcg cagcctgaat ggcgcccgct cctttcgctt tcttcccttc ctttctcgcc

     6301 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt

     6361 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg

     6421 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt

     6481 ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta

     6541 taagggattt tgccgatttc ggaaccacca tcaaacagga ttttcgcctg ctggggcaaa

     6601 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt

     6661 tgcccgtctc actggtgaaa agaaaaacca ccccagtaca ttaaaaacgt ccgcaatgtg

     6721 ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc

     6781 caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca

     6841 gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg

     6901 atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga

     6961 gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa atatcatctc

     7021 cctcgcagag atccgaatta tcagccttct tattcatttc tcgcttaacc gtgacaggct

     7081 gtcgatcttg agaactatgc cgacataata ggaaatcgct ggataaagcc gctgaggaag

     7141 ctgagtggcg ctatttcttt agaagtgaac gttgacgata tcaactcccc tatccattgc

     7201 tcaccgaatg gtacaggtcg gggacccgaa gttccgactg tcggcctgat gcatccccgg

     7261 ctgatcgacc ccagatctgg ggctgagaaa gcccagtaag gaaacaactg taggttcgag

     7321 tcgcgagatc ccccggaacc aaaggaagta ggttaaaccc gctccgatca ggccgagcca

     7381 cgccaggccg agaacattgg ttcctgtagg catcgggatt ggcggatcaa acactaaagc

     7441 tactggaacg agcagaagtc ctccggccgc cagttgccag gcggtaaagg tgagcagagg

     7501 cacgggaggt tgccacttgc gggtcagcac ggttccgaac gccatggaaa ccgcccccgc

     7561 caggcccgct gcgacgccga caggatctag cgctgcgttt ggtgtcaaca ccaacagcgc

     7621 cacgcccgca gttccgcaaa tagcccccag gaccgccatc aatcgtatcg ggctacctag

     7681 cagagcggca gagatgaaca cgaccatcag cggctgcaca gcgcctaccg tcgccgcgac

     7741 cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg aaatattaag

     7801 tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc ggacgatcat

     7861 cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg

     7921 ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc

     7981 ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac

     8041 ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac

     8101 ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg

     8161 cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc

     8221 tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa

     8281 gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc

     8341 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc

     8401 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg

     8461 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg

     8521 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac

     8581 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg

     8641 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg

     8701 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc

     8761 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc

     8821 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg

     8881 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc

     8941 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc

     9001 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg

     9061 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt

     9121 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt

     9181 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg

     9241 atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg

     9301 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg

     9361 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg

     9421 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct

     9481 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg

     9541 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc

     9601 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg

     9661 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg

     9721 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc

     9781 ttgtcgccgg catcgttgcg ccacatctag gtactaaaac aattcatcca gtaaaatata

     9841 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata

     9901 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt

     9961 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa

    10021 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt

    10081 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc

    10141 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc

    10201 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc

    10261 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag

    10321 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag

    10381 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac

    10441 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc

    10501 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta

    10561 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct

    10621 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc

    10681 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg

    10741 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaattatgg

    10801 gtgatgctgc caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct

    10861 tctgtgtcta tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa

    10921 agcaccgccg gacatcagcg ctatctctgc tctcactgcc gtaaaacatg gcaactgcag

    10981 ttcacttaca ccgcttctca acccggtacg caccagaaaa tcattgatat ggccatgaat

    11041 ggcgttggat gccgggcaac agcccgcatt atgggcgttg gcctcaacac gattttacgt

    11101 cacttaaaaa actcaggccg cagtcggtaa cctcgcgcat acagccgggc agtgacgtca

    11161 tcgtctgcgc ggaaatggac gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct

    11221 ggctgtttta cgcgtatgac agtctccgga agacggttgt tgcgcacgta ttcggtgaac

    11281 gcactatggc gacgctgggg cgtcttatga gcctgctgtc accctttgac gtggtgatat

    11341 ggatgacgga tggctggccg ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca

    11401 gcaagcgata tacgcagcga attgagcggc ataacctgaa tctgaggcag cacctggcac

    11461 ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg

    11521 ggcattatct gaacataaaa cactatcaat aagttggagt cattacccaa ttatgataga

    11581 atttacaagc tataaggtta ttgtcctggg tttcaagcat tagtccatgc aagtttttat

    11641 gctttgccca ttctatagat atattgataa gcgcgctgcc tatgccttgc cccctgaaat

    11701 ccttacatac ggcgatatct tctatataaa agatatatta tcttatcagt attgtcaata

    11761 tattcaaggc aatctgcctc ctcatcctct tcatcctctt cgtcttggta gctttttaaa

    11821 tatggcgctt catagagtaa ttctgtaaag gtccaattct cgtttcatac ctcggtataa

    11881 tcttacctat cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc

    11941 acccgcgacc actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc

    12001 cgtgaatgcc ccgacggccg aagtgaaggg caggccgcca ccaggccgcc gccctcactg

    12061 cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg gcacgtcaat gcttccgggc

    12121 gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga tcccggcaat ggcaaggact

    12181 gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag cccctggggg

    12241 gatgggaggc ccgcgttagc gggccgggag ggttcgagaa gggggggcac cccccttcgg

    12301 cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa acaaggttta taaatattgg

    12361 tttaaaagca ggttaaaaga caggttagcg gtggccgaaa aacgggcgga aacccttgca

    12421 aatgctggat tttctgcctg tggacagccc ctcaaatgtc aataggtgcg cccctcatct

    12481 gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct atctgtcagt agtcgcgccc

    12541 ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa

    12601 ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc

    12661 cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg

    12721 tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc

    12781 cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca

    12841 tagacggccg ccagcccagc ggcgagggca accagcccgg

//

 

 

pROKII载体其他相关的植物过表达载体:

pUC-SPYNE
pUC-SPYCE
pUC-35S-mCherry
pSoup+p19
pROKII
pGreenII 0800-Luc
pGreenII 62-SK
pER8
pCAMBIA3300
pCAMBIA1301-35SN
pBI221-EGFP
pBI121-mCherry
pBI121-EGFP
pBI121-DsRed2
pA7-CFP
CaMV35S-sGFP
pGWB504
pDONR221/Zeo
pDONR221/Kan
K7GWIWG2 II-RedRoot
pTRV2
pTRV1
pROKII-RNAI