pCAMBIA1301-35SN
pCAMBIA1301-35SN
编号 | 载体名称 |
北京华越洋VECT75572 | pCAMBIA1301-35SN |
pCAMBIA1301-35SN载体基本信息:
启动子: | Lac,CaMV 35S |
复制子: | pVS1 oriV,ori |
质粒分类: | 植物系列,蛋白过表达载体 |
质粒大小: | 11849bp |
原核抗性: | Kan |
筛选标记: | Hyg |
克隆菌株: | DH5α |
培养条件: | 37℃,有氧 LB |
表达宿主: | 植物细胞 |
5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
3'测序引物: | 根据序列设计引物 |
pCAMBIA1301-35SN载体简介:
This vector contains a fully functional gusA reporter construct for simple and sensitive analysis of gene function or presence in regenerated plants by GUS assay. The construct uses E.coli gusA with an intron (from the castor bean catalase gene) inside the coding sequence to ensure that expression of glucuronidase activity is derived from eukaryotic cells, not from expression by residual A.tumefaciens cells. This vector is suitable for insertion of other genes of interest containing their own promoter and terminator. Researchers can excise the gusA gene and insert their own gene of interest in its place or use these vectors to create fusions of gusA with their gene of interest. These vectors contain the pUC18 polylinker-lacZa.
pCAMBIA1301-35SN载体其他相关的植物过表达载体: