北京华越洋生物
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首页    载体及质粒    pBI121-EGFP
pBI121-EGFP1

pBI121-EGFP

pBI121-EGFP

pBI121-EGFP



 

编号

载体名称

北京华越洋VECT75585

pBI121-EGFP

 

pBI121-EGFP载体基本信息:

启动子:

CaMV 35S

复制子:

oriV

终止子:

NOS

质粒分类:

植物系列,蛋白过表达载体

质粒大小:

13832bp

质粒标签:

N-10*His, N-EGFP,C-6*His

原核抗性:

Kan

筛选标记:

Neo

克隆菌株:

DH5α

培养条件:

37℃,有氧 LB

5'测序引物:

35S:GACGCACAATCCCACTATCC

3'测序引物:

根据序列设计引物

 

pBI121-EGFP载体图谱:


pBI121-EGFP载体简介:

pBI121-EGFP载体是由采用pBI121和EGFP标签改造而来,是植物表达载体,带有Kanamycin卡纳霉素抗性,可转化进大肠杆菌DH5α,通过Kan筛选大量复制扩增,培养条件是LB,37℃。pBI121-EGFP载体的启动子是35s,可以通过农杆菌介导在植物细胞中表达EGFP绿色荧光蛋白,标签有N-10*His, N-EGFP,C-6*His,可选用镍柱纯化。pBI121-EGFP的酶切位点有XhoI,NcoI,SalI,SpeI,XbaI,SmaI,BamHI,SacI,EcoRI,可根据需求适当选用酶切位点.

 

pBI121-EGFP载体序列:

ORIGIN

        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc

       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc

      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat

      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc

      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag

      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc

      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc

      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg

      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc

      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata

      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg

      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata

      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta

      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg

      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt

      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag

      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt

     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta

     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt

     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg

     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga

     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg

     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac

     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc

     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg

     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc

     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc

     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg

     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg

     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg

     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg

     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc

     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa

     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg

     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa

     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc

     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc

     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg

     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc

     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct

     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc

     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga

     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc

     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc

     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc

     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa

     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg

     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt

     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg

     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg

     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg

     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg

     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca

     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc

     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc

     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca

     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga

     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg

     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg

     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg

     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga

     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag

     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct

     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat

     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg

     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg

     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc

     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc

     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg

     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata

     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc

     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg

     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag

     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta

     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca

     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt

     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg

     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt

     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc

     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag

     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt

     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca

     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag

     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg

     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa

     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat

     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc

     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat

     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt

     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca

     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat

     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat

     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg

     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc

     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt

     5701 ggattgatgt gatatctcca atgacgtaag ggatgacgca caatcccact atccttcgca

     5761 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga cctcaacaca

     5821 acatatacaa aacaaacgaa tctcaagcaa tcaagcattc tacttctatt gcagcaattt

     5881 aaatcatttc ttttaaagca aaagcaattt tctgaaaatt ttcaccattt acgaacgata

     5941 ctcgagatga cccgtggttc tcatcaccat caccatcacc atcaccatca cgccatggtc

     6001 gacgtactag tgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg

     6061 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc

     6121 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg

     6181 ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc

     6241 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag

     6301 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag

     6361 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac

     6421 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac

     6481 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc

     6541 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg

     6601 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc

     6661 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag

     6721 ctggacaagg gtctagaccc gggaatgcat caccatcacc atcacggatc ctgattgatc

     6781 gatagagctc gaatttcccc gatcgatcaa acatttggca ataaagtttc ttaagattga

     6841 atcctgttgc cgggcttgcg atgattatca tataatttct gttgaattac gttaaacatg

     6901 aaataattaa catgtaatgc atgacgttat ttatgatatg ggtttttatg attatagtcc

     6961 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac tacgataaat

     7021 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacaa

     7081 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct

     7141 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc

     7201 agcctgaatg gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg

     7261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg

     7321 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg

     7381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt

     7441 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt

     7501 gccgatttcg gaaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac

     7561 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca

     7621 ctggtgaaaa gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg

     7681 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc

     7741 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg

     7801 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg

     7861 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt

     7921 tgattgtaac gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga

     7981 tccgaattat cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga

     8041 gaactatgcc gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc

     8101 tatttcttta gaagtgaacg ttgacgatat caactcccct atccattgct caccgaatgg

     8161 tacaggtcgg ggacccgaag ttccgactgt cggcctgatg catccccggc tgatcgaccc

     8221 cagatctggg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc

     8281 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga

     8341 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga

     8401 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt

     8461 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg

     8521 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag

     8581 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag

     8641 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca

     8701 ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga

     8761 tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata

     8821 aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct

     8881 ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga

     8941 agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca

     9001 accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca

     9061 tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg

     9121 ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg

     9181 gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc

     9241 agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac

     9301 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc

     9361 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt

     9421 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg

     9481 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta

     9541 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc

     9601 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag

     9661 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg

     9721 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag

     9781 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc

     9841 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt

     9901 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg

     9961 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc

    10021 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg

    10081 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc

    10141 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga

    10201 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg

    10261 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg

    10321 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct

    10381 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc

    10441 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg

    10501 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac

    10561 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt

    10621 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc

    10681 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc

    10741 atcgttgcgc cacatctagg tactaaaaca attcatccag taaaatataa tattttattt

    10801 tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc

    10861 cgatatcctc cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc

    10921 cgcttctccc aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt

    10981 ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat

    11041 catacagctc gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat

    11101 cggccagatc gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg

    11161 tatagggaca atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct

    11221 cgataatctt ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg

    11281 cctcactcat gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa

    11341 caggcagctt tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg

    11401 tccctttata ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct

    11461 tatatacctt agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca

    11521 gttttttcaa ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct

    11581 acagtattta aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt

    11641 ccttgcattc taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt

    11701 ggcgtataac atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc

    11761 aacttactga tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtgtctat

    11821 cagctgtccc tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg

    11881 acatcagcgc tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac

    11941 cgcttctcaa cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg

    12001 ccgggcaaca gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa

    12061 ctcaggccgc agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg

    12121 gaaatggacg aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac

    12181 gcgtatgaca gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg

    12241 acgctggggc gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat

    12301 ggctggccgc tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat

    12361 acgcagcgaa ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg

    12421 aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg

    12481 aacataaaac actatcaata agttggagtc attacccaat tatgatagaa tttacaagct

    12541 ataaggttat tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat

    12601 tctatagata tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg

    12661 gcgatatctt ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca

    12721 atctgcctcc tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc

    12781 atagagtaat tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat

    12841 cacctcaaat ggttcgctgg gtttatcgca cccccgaaca cgagcacggc acccgcgacc

    12901 actatgccaa gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc

    12961 ccgacggccg aagtgaaggg caggccgcca cccaggccgc cgccctcact gcccggcacc

    13021 tggtcgctga atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc

    13081 gggctgatcg cccatcccgt tactgccccg atcccggcaa tggcaaggac tgccagcgct

    13141 gccatttttg gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg

    13201 cccgcgttag cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg

    13261 tcacgcgcac agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc

    13321 aggttaaaag acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga

    13381 ttttctgcct gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact

    13441 ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg

    13501 tcaataccgc agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta

    13561 aaatcaggcg ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg

    13621 agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc

    13681 cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc

    13741 gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc

    13801 cgccagccca gcggcgaggg caaccagccc gg

//

 

 

pBI121-EGFP载体其他相关的植物过表达载体:

pUC-SPYNE
pUC-SPYCE
pUC-35S-mCherry
pSoup+p19
pROKII
pGreenII 0800-Luc
pGreenII 62-SK
pER8
pCAMBIA3300
pCAMBIA1301-35SN
pBI221-EGFP
pBI121-mCherry
pBI121-EGFP
pBI121-DsRed2
pA7-CFP
CaMV35S-sGFP
pGWB504
pDONR221/Zeo
pDONR221/Kan
K7GWIWG2 II-RedRoot
pTRV2
pTRV1
pROKII-RNAI