pVAX1-FLAG

ꄴ上一个： pGAPZA

ꄲ下一个： pSV2-DHFR

pVAX1-FLAG

编号	载体名称
北京华越洋VECT90221	pVAX1-FLAG

pVAX1-FLAG载体基本信息：

启动子:	CMV
复制子:	pUC ori
终止子:	BGH poly（A）signal
质粒分类:	哺乳细胞载体;蛋白过表达质粒
质粒大小:	3023bp
原核抗性:	卡那霉素Kan
筛选标记:	新霉素Neo
克隆菌株:	DH5α
培养条件:	37℃，有氧，LB
表达宿主:	哺乳细胞

pVAX1-FLAG载体质粒图谱和多克隆位点信息:

pVAX1-FLAG载体简介

pVAX1-FLAG载体序列：

ORIGIN
        1 GACTCTTCGC GATGTACGGG CCAGATATAC GCGTTGACAT TGATTATTGA CTAGTTATTA
       61 ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC GCGTTACATA
      121 ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT TGACGTCAAT
      181 AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA
      241 CTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC
      301 CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TATGCCCAGT ACATGACCTT
      361 ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTGAT
      421 GCGGTTTTGG CAGTACATCA ATGGGCGTGG ATAGCGGTTT GACTCACGGG GATTTCCAAG
      481 TCTCCACCCC ATTGACGTCA ATGGGAGTTT GTTTTGGCAC CAAAATCAAC GGGACTTTCC
      541 AAAATGTCGT AACAACTCCG CCCCATTGAC GCAAATGGGC GGTAGGCGTG TACGGTGGGA
      601 GGTCTATATA AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA
      661 AATTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT
      721 ACCGAGCTCG GATCCACTAG TCCAGTGTGG TGGAATTCTG CAGATATCCA GCACAGTGGC
      781 GGCCGCTCGA GGACTACAAG GACGACGATG ACAAGTCTAG AGGGCCCGTT TAAACCCGCT
      841 GATCAGCCTC GACTGTGCCT TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC
      901 CTTCCTTGAC CCTGGAAGGT GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG
      961 CATCGCATTG TCTGAGTAGG TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA
     1021 AGGGGGAGGA TTGGGAAGAC AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT
     1081 CTACTGGGCG GTTTTATGGA CAGCAAGCGA ACCGGAATTG CCAGCTGGGG CGCCCTCTGG
     1141 TAAGGTTGGG AAGCCCTGCA AAGTAAACTG GATGGCTTTC TCGCCGCCAA GGATCTGATG
     1201 GCGCAGGGGA TCAAGCTCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA
     1261 AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG
     1321 GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG
     1381 CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC
     1441 AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT
     1501 CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC
     1561 ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA
     1621 TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC
     1681 ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG
     1741 GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT
     1801 CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC
     1861 TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC
     1921 TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA
     1981 CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT
     2041 CTGAATTATT AACGCTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG
     2101 TATTTCACAC CGCATACAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT
     2161 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG
     2221 CTTCAATAAT AGCACGTGCT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC
     2281 CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA
     2341 GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC
     2401 TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA
     2461 CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT
     2521 CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC
     2581 GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG
     2641 TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG
     2701 TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG
     2761 CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC
     2821 AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT
     2881 AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG
     2941 GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGGCTTTTGC
     3001 TGGCCTTTTG CTCACATGTT CTT
//

pVAX1-FLAG其他相关哺乳细胞载体：

pEF1/V5-HisA	pEGFP-N3
pEF-GFP	pEGFP-C3
pFLAG-CMV2	pEGFP-N2
pCI-NEO	pEGFP-N1
pCEP4	pEGFP-1
pVAX1	pECFP-C1
pcDNA3.1/V5-His B	pECFP-N1
pCDNA6-Myc-HisB	pDsRed2-Nuc
pCDNA3.1-3×HA	pDsRed2-ER
pCDNA3.1-3×HA	Top-Flash
pCDNA3.1(-)/Myc-His B	pSTAT3-TA-luc
pCDNA3.1(-)/Myc-His A	pSilencer2.1-U6 neo
pCDNA3.1(+)/Myc-His B	psiCHECK-2
pCDNA3.1(+)-Zeo	pRL-TK
pCDNA3.1(+)-EGFP	pRL-SV40
pCDNA3.1(+)	pRL-CMV
pCDNA3.1(-)	pp53-TA-luc
pBudCE4.1-hrGFP	pNF-KB-TA-LUC
pCAGGS-HA	pNF-kB-Luc
pVAX1-FLAG	pMIR-Reporter
pSV2-DHFR	pmirGLO
pCEP4/CAT	pGL4.79[hRluc/Neo]
pCAGGS	pGL4.75[hRluc/CMV]
pCAG-GFP	pGL4.73[hRluc /SV40]
pCDNA3.0	pGL4.29[luc2P/CRE/Hygro]
pCDNA3.0-HA	pGL4.13-luci2
pCDNA3.1(+)-3×FLAG	pGL4.13-luci2
pSV-Beta-Gal	pGL4.10[luc2]
SuperCos1	pGL3-Enhancer
pT2/VENUS	pGL3-Control
pAdVAntage	pGL3-Basic
pEYFP-Tubulin	pGL3-3.6kb human IFN-gamma luc
pEGFP-C1	pENTR5'-UbCp
pmGeos-M-N1	pBiFC-VN173
pEYFP-N1	pBiFC-VC155
pEYFP-C1	pBIFC-bFosVC155
pEGFP-C2	pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP) VC155
pEYFP-Actin	Laconic/pcDNA3.1(-)
pDsRed2-Peroxi	Fop-Flash
pEBFP-C1	pTet-On-Advanced
pmGeos-M-C1	pmCherry-EGFP-LC3b-r
pCerulean-N1	pmCherry-EGFP-LC3b-m
pmKO2-N1	pmCherry-EGFP-LC3B-h
pMyosin-PSmOrange	pEGFP-LC3B-r
pTagBFP-N	pEGFP-LC3B-m
pCAG-DsRed	pEGFP-LC3B-h
pmCherry-C1	pEGFP-LC3B-c
pmCherry-N1	Super PiggyBac Transposase (PB200PA-1)
pEYFP-Golgi	pTol2-Promoter-T2A-EGFP
pEYFP-Mem	pSP64-Tol2-Transposase
pDsRed2-C1	PiggyBac Dual promoter (PB513B-1)
pEGFP-Actin	pBIND-ID
pEBFP-N1	pG5Luc
pDsRed2-N1	pFUSE-hIgG1e1-Fc2
pN3	pEE12.4
pEGFP-EcoRV	pEE6.4