北京华越洋生物
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7*24h全国热线:
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首页    载体及质粒    pCEP4/CAT
pCEP4-CAT

pCEP4/CAT

pCEP4/CAT

pCEP4/CAT

 



编号

载体名称

北京华越洋VECT90223

pCEP4/CAT

 

pCEP4/CAT载体基本信息:

启动子:

CMV

复制子:

pUC ori

终止子:

SV40 poly(A) signal

质粒分类:

哺乳细胞,蛋白过表达载体

质粒大小:

10891bp

原核抗性:

Amp

筛选标记:

Hyg

克隆菌株:

DH5α

培养条件:

37℃,有氧,LB

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:

CMV-FCGCAAATGGGCGGTAGGCGTG

3'测序引物:

Sv40-polyA-RGAAATTTGTGATGCTATTGC

 

pCEP4/CAT载体质粒图谱和多克隆位点信息:

 

 

pCEP4/CAT载体简介:

 pCEP4 is an episomal mammalian expression vector that uses thecytomegalovirus (CMV) immediate early enhancer/promoter for high level transcription of recombinant genes inserted into the multiple cloning site. The Epstein-Barr Virus replication origin (oriP) and nuclear antigen (encoded by the EBNA-1 gene) is carried by this plasmid to permit extrachromosomal replication in human, primate, and canine cells. pCEP4 also carries the hygromycin B resistance gene for stable selection in transfected cells.

 

pCEP4/CAT is provided as a positive control for the relative level of expression of recombinant proteins in a cell line of interest. It expresses the chloramphenicol acetyl transferase (CAT) protein from the CMV enhancer/promoter. Like its parent vector pCEP4, pCEP4/CAT contains the hygromycin B resistance gene for selection.

 

 pCEP4pCEP4/CAT的对照质粒,插入一个基因CAT.

 

pCEP4/CAT载体序列:

ORIGIN

        1 GTTGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA

       61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC

      121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG

      181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC

      241 ATCAAGTGTA TCATATGCCA AGTCCGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG

      301 CCTGGCATTA TGCCCAGTAC ATGACCTTAC GGGACTTTCC TACTTGGCAG TACATCTACG

      361 TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACACCAAT GGGCGTGGAT

      421 AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT

      481 TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA TAACCCCGCC CCGTTGACGC

      541 AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG CAGAGCTCGT TTAGTGAACC

      601 GTCAGATCTC TAGAAGCTGG GTACCAGCTG CTAGCAAGCT TGGCTAGCGG CCGCCAGGAG

      661 CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT ATATCCCAAT

      721 GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC TATAACCAGA

      781 CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG CACAAGTTTT

      841 ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA TTCCGTATGG

      901 CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC ACCGTTTTCC

      961 ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT TTCCGGCAGT

     1021 TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC TATTTCCCTA

     1081 AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT TTCACCAGTT

     1141 TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC ATGGGCAAAT

     1201 ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT CATGCCGTCT

     1261 GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTACTGC GATGAGTGGC

     1321 AGGGCGGGGC GTAATTTTTT TAAGGCAGTT ATTGGTGCCC TTGGCCGGCA AGGCCGGATC

     1381 CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA

     1441 AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA

     1501 ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT

     1561 GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCCGG

     1621 CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC

     1681 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG CGTCAGCGGG

     1741 TGTTGGCGGG TGTCGGGGCG CAGCCATGAG GTCGACTCTA GAGGATCGAT GCCCCGCCCC

     1801 GGACGAACTA AACCTGACTA CGACATCTCT GCCCCTTCTT CGCGGGGCAG TGCATGTAAT

     1861 CCCTTCAGTT GGTTGGTACA ACTTGCCAAC TGGGCCCTGT TCCACATGTG ACACGGGGGG

     1921 GGACCAAACA CAAAGGGGTT CTCTGACTGT AGTTGACATC CTTATAAATG GATGTGCACA

     1981 TTTGCCAACA CTGAGTGGCT TTCATCCTGG AGCAGACTTT GCAGTCTGTG GACTGCAACA

     2041 CAACATTGCC TTTATGTGTA ACTCTTGGCT GAAGCTCTTA CACCAATGCT GGGGGACATG

     2101 TACCTCCCAG GGGCCCAGGA AGACTACGGG AGGCTACACC AACGTCAATC AGAGGGGCCT

     2161 GTGTAGCTAC CGATAAGCGG ACCCTCAAGA GGGCATTAGC AATAGTGTTT ATAAGGCCCC

     2221 CTTGTTAACC CTAAACGGGT AGCATATGCT TCCCGGGTAG TAGTATATAC TATCCAGACT

     2281 AACCCTAATT CAATAGCATA TGTTACCCAA CGGGAAGCAT ATGCTATCGA ATTAGGGTTA

     2341 GTAAAAGGGT CCTAAGGAAC AGCGATATCT CCCACCCCAT GAGCTGTCAC GGTTTTATTT

     2401 ACATGGGGTC AGGATTCCAC GAGGGTAGTG AACCATTTTA GTCACAAGGG CAGTGGCTGA

     2461 AGATCAAGGA GCGGGCAGTG AACTCTCCTG AATCTTCGCC TGCTTCTTCA TTCTCCTTCG

     2521 TTTAGCTAAT AGAATAACTG CTGAGTTGTG AACAGTAAGG TGTATGTGAG GTGCTCGAAA

     2581 ACAAGGTTTC AGGTGACGCC CCCAGAATAA AATTTGGACG GGGGGTTCAG TGGTGGCATT

     2641 GTGCTATGAC ACCAATATAA CCCTCACAAA CCCCTTGGGC AATAAATACT AGTGTAGGAA

     2701 TGAAACATTC TGAATATCTT TAACAATAGA AATCCATGGG GTGGGGACAA GCCGTAAAGA

     2761 CTGGATGTCC ATCTCACACG AATTTATGGC TATGGGCAAC ACATAATCCT AGTGCAATAT

     2821 GATACTGGGG TTATTAAGAT GTGTCCCAGG CAGGGACCAA GACAGGTGAA CCATGTTGTT

     2881 ACACTCTATT TGTAACAAGG GGAAAGAGAG TGGACGCCGA CAGCAGCGGA CTCCACTGGT

     2941 TGTCTCTAAC ACCCCCGAAA ATTAAACGGG GCTCCACGCC AATGGGGCCC ATAAACAAAG

     3001 ACAAGTGGCC ACTCTTTTTT TTGAAATTGT GGAGTGGGGG CACGCGTCAG CCCCCACACG

     3061 CCGCCCTGCG GTTTTGGACT GTAAAATAAG GGTGTAATAA CTTGGCTGAT TGTAACCCCG

     3121 CTAACCACTG CGGTCAAACC ACTTGCCCAC AAAACCACTA ATGGCACCCC GGGGAATACC

     3181 TGCATAAGTA GGTGGGCGGG CCAAGATAGG GGCGCGATTG CTGCGATCTG GAGGACAAAT

     3241 TACACACACT TGCGCCTGAG CGCCAAGCAC AGGGTTGTTG GTCCTCATAT TCACGAGGTC

     3301 GCTGAGAGCA CGGTGGGCTA ATGTTGCCAT GGGTAGCATA TACTACCCAA ATATCTGGAT

     3361 AGCATATGCT ATCCTAATCT ATATCTGGGT AGCATAGGCT ATCCTAATCT ATATCTGGGT

     3421 AGCATATGCT ATCCTAATCT ATATCTGGGT AGTATATGCT ATCCTAATTT ATATCTGGGT

     3481 AGCATAGGCT ATCCTAATCT ATATCTGGGT AGCATATGCT ATCCTAATCT ATATCTGGGT

     3541 AGTATATGCT ATCCTAATCT GTATCCGGGT AGCATATGCT ATCCTAATAG AGATTAGGGT

     3601 AGTATATGCT ATCCTAATTT ATATCTGGGT AGCATATACT ACCCAAATAT CTGGATAGCA

     3661 TATGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGCA

     3721 TAGGCTATCC TAATCTATAT CTGGGTAGCA TATGCTATCC TAATCTATAT CTGGGTAGTA

     3781 TATGCTATCC TAATTTATAT CTGGGTAGCA TAGGCTATCC TAATCTATAT CTGGGTAGCA

     3841 TATGCTATCC TAATCTATAT CTGGGTAGTA TATGCTATCC TAATCTGTAT CCGGGTAGCA

     3901 TATGCTATCC TCATGCATAT ACAGTCAGCA TATGATACCC AGTAGTAGAG TGGGAGTGCT

     3961 ATCCTTTGCA TATGCCGCCA CCTCCCAAGG GGGCGTGAAT TTTCGCTGCT TGTCCTTTTC

     4021 CTGCTGGTTG CTCCCATTCT TAGGTGAATT TAAGGAGGCC AGGCTAAAGC CGTCGCATGT

     4081 CTGATTGCTC ACCAGGTAAA TGTCGCTAAT GTTTTCCAAC GCGAGAAGGT GTTGAGCGCG

     4141 GAGCTGAGTG ACGTGACAAC ATGGGTATGC CCAATTGCCC CATGTTGGGA GGACGAAAAT

     4201 GGTGACAAGA CAGATGGCCA GAAATACACC AACAGCACGC ATGATGTCTA CTGGGGATTT

     4261 ATTCTTTAGT GCGGGGGAAT ACACGGCTTT TAATACGATT GAGGGCGTCT CCTAACAAGT

     4321 TACATCACTC CTGCCCTTCC TCACCCTCAT CTCCATCACC TCCTTCATCT CCGTCATCTC

     4381 CGTCATCACC CTCCGCGGCA GCCCCTTCCA CCATAGGTGG AAACCAGGGA GGCAAATCTA

     4441 CTCCATCGTC AAAGCTGCAC ACAGTCACCC TGATATTGCA GGTAGGAGCG GGCTTTGTCA

     4501 TAACAAGGTC CTTAATCGCA TCCTTCAAAA CCTCAGCAAA TATATGAGTT TGTAAAAAGA

     4561 CCATGAAATA ACAGACAATG GACTCCCTTA GCGGGCCAGG TTGTGGGCCG GGTCCAGGGG

     4621 CCATTCCAAA GGGGAGACGA CTCAATGGTG TAAGACGACA TTGTGGAATA GCAAGGGCAG

     4681 TTCCTCGCCT TAGGTTGTAA AGGGAGGTCT TACTACCTCC ATATACGAAC ACACCGGCGA

     4741 CCCAAGTTCC TTCGTCGGTA GTCCTTTCTA CGTGACTCCT AGCCAGGAGA GCTCTTAAAC

     4801 CTTCTGCAAT GTTCTCAAAT TTCGGGTTGG AACCTCCTTG ACCACGATGC TTTCCAAACC

     4861 ACCCTCCTTT TTTGCGCCTG CCTCCATCAC CCTGACCCCG GGGTCCAGTG CTTGGGCCTT

     4921 CTCCTGGGTC ATCTGCGGGG CCCTGCTCTA TCGCTCCCGG GGGCACGTCA GGCTCACCAT

     4981 CTGGGCCACC TTCTTGGTGG TATTCAAAAT AATCGGCTTC CCCTACAGGG TGGAAAAATG

     5041 GCCTTCTACC TGGAGGGGGC CTGCGCGGTG GAGACCCGGA TGATGATGAC TGACTACTGG

     5101 GACTCCTGGG CCTCTTTTCT CCACGTCCAC GACCTCTCCC CCTGGCTCTT TCACGACTTC

     5161 CCCCCCTGGC TCTTTCACGT CCTCTACCCC GGCGGCCTCC ACTACCTCCT CGACCCCGGC

     5221 CTCCACTACC TCCTCGACCC CGGCCTCCAC TGCCTCCTCG ACCCCGGCCT CCACCTCCTG

     5281 CTCCTGCCCC TCCTGCTCCT GCCCCTCCTC CTGCTCCTGC CCCTCCTGCC CCTCCTGCTC

     5341 CTGCCCCTCC TGCCCCTCCT GCTCCTGCCC CTCCTGCCCC TCCTGCTCCT GCCCCTCCTG

     5401 CCCCTCCTCC TGCTCCTGCC CCTCCTGCCC CTCCTCCTGC TCCTGCCCCT CCTGCCCCTC

     5461 CTGCTCCTGC CCCTCCTGCC CCTCCTGCTC CTGCCCCTCC TGCCCCTCCT GCTCCTGCCC

     5521 CTCCTGCTCC TGCCCCTCCT GCTCCTGCCC CTCCTGCTCC TGCCCCTCCT GCCCCTCCTG

     5581 CCCCTCCTCC TGCTCCTGCC CCTCCTGCTC CTGCCCCTCC TGCCCCTCCT GCCCCTCCTG

     5641 CTCCTGCCCC TCCTCCTGCT CCTGCCCCTC CTGCCCCTCC TGCCCCTCCT CCTGCTCCTG

     5701 CCCCTCCTGC CCCTCCTCCT GCTCCTGCCC CTCCTCCTGC TCCTGCCCCT CCTGCCCCTC

     5761 CTGCCCCTCC TCCTGCTCCT GCCCCTCCTG CCCCTCCTCC TGCTCCTGCC CCTCCTCCTG

     5821 CTCCTGCCCC TCCTGCCCCT CCTGCCCCTC CTCCTGCTCC TGCCCCTCCT CCTGCTCCTG

     5881 CCCCTCCTGC CCCTCCTGCC CCTCCTGCCC CTCCTCCTGC TCCTGCCCCT CCTCCTGCTC

     5941 CTGCCCCTCC TGCTCCTGCC CCTCCCGCTC CTGCTCCTGC TCCTGTTCCA CCGTGGGTCC

     6001 CTTTGCAGCC AATGCAACTT GGACGTTTTT GGGGTCTCCG GACACCATCT CTATGTCTTG

     6061 GCCCTGATCC TGAGCCGCCC GGGGCTCCTG GTCTTCCGCC TCCTCGTCCT CGTCCTCTTC

     6121 CCCGTCCTCG TCCATGGTTA TCACCCCCTC TTCTTTGAGG TCCACTGCCG CCGGAGCCTT

     6181 CTGGTCCAGA TGTGTCTCCC TTCTCTCCTA GGCCATTTCC AGGTCCTGTA CCTGGCCCCT

     6241 CGTCAGACAT GATTCACACT AAAAGAGATC AATAGACATC TTTATTAGAC GACGCTCAGT

     6301 GAATACAGGG AGTGCAGACT CCTGCCCCCT CCAACAGCCC CCCCACCCTC ATCCCCTTCA

     6361 TGGTCGCTGT CAGACAGATC CAGGTCTGAA AATTCCCCAT CCTCCGAACC ATCCTCGTCC

     6421 TCATCACCAA TTACTCGCAG CCCGGAAAAC TCCCGCTGAA CATCCTCAAG ATTTGCGTCC

     6481 TGAGCCTCAA GCCAGGCCTC AAATTCCTCG TCCCCCTTTT TGCTGGACGG TAGGGATGGG

     6541 GATTCTCGGG ACCCCTCCTC TTCCTCTTCA AGGTCACCAG ACAGAGATGC TACTGGGGCA

     6601 ACGGAAGAAA AGCTGGGTGC GGCCTGTGAG GATCAGCTTA TCGATGATAA GCTGTCAAAC

     6661 ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA

     6721 TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC

     6781 CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT

     6841 GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG

     6901 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG

     6961 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC

     7021 TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA

     7081 CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC

     7141 TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA

     7201 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG

     7261 ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT

     7321 TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG

     7381 AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC

     7441 GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA

     7501 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA

     7561 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC

     7621 CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG

     7681 ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT

     7741 CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA

     7801 GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT

     7861 CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT

     7921 TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT

     7981 TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA

     8041 TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG

     8101 CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA

     8161 AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG

     8221 GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA

     8281 GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA

     8341 GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA

     8401 ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT

     8461 TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC

     8521 GGTTCCTGGC CTTTTGCTGG CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG

     8581 ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG

     8641 GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCC

     8701 CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG

     8761 GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT

     8821 GAATCCGTTA GCGAGGTGCC GCCCTGCTTC ATCCCCGTGG CCCGTTGCTC GCGTTTGCTG

     8881 GCGGTGTCCC CGGAAGAAAT ATATTTGCAT GTCTTTAGTT CTATGATGAC ACAAACCCCG

     8941 CCCAGCGTCT TGTCATTGGC GAATTCGAAC ACGCAGATGC AGTCGGGGCG GCGCGGTCCG

     9001 AGGTCCACTT CGCATATTAA GGTGACGCGT GTGGCCTCGA ACACCGAGCG ACCCTGCAGC

     9061 GACCCGCTTA ACAGCGTCAA CAGCGTGCCG CAGATCCCGG GGGGCAATGA GATATGAAAA

     9121 AGCCTGAACT CACCGCGACG TCTGTCGAGA AGTTTCTGAT CGAAAAGTTC GACAGCGTCT

     9181 CCGACCTGAT GCAGCTCTCG GAGGGCGAAG AATCTCGTGC TTTCAGCTTC GATGTAGGAG

     9241 GGCGTGGATA TGTCCTGCGG GTAAATAGCT GCGCCGATGG TTTCTACAAA GATCGTTATG

     9301 TTTATCGGCA CTTTGCATCG GCCGCGCTCC CGATTCCGGA AGTGCTTGAC ATTGGGGAAT

     9361 TCAGCGAGAG CCTGACCTAT TGCATCTCCC GCCGTGCACA GGGTGTCACG TTGCAAGACC

     9421 TGCCTGAAAC CGAACTGCCC GCTGTTCTGC AGCCGGTCGC GGAGGCCATG GATGCGATCG

     9481 CTGCGGCCGA TCTTAGCCAG ACGAGCGGGT TCGGCCCATT CGGACCGCAA GGAATCGGTC

     9541 AATACACTAC ATGGCGTGAT TTCATATGCG CGATTGCTGA TCCCCATGTG TATCACTGGC

     9601 AAACTGTGAT GGACGACACC GTCAGTGCGT CCGTCGCGCA GGCTCTCGAT GAGCTGATGC

     9661 TTTGGGCCGA GGACTGCCCC GAAGTCCGGC ACCTCGTGCA CGCGGATTTC GGCTCCAACA

     9721 ATGTCCTGAC GGACAATGGC CGCATAACAG CGGTCATTGA CTGGAGCGAG GCGATGTTCG

     9781 GGGATTCCCA ATACGAGGTC GCCAACATCT TCTTCTGGAG GCCGTGGTTG GCTTGTATGG

     9841 AGCAGCAGAC GCGCTACTTC GAGCGGAGGC ATCCGGAGCT TGCAGGATCG CCGCGGCTCC

     9901 GGGCGTATAT GCTCCGCATT GGTCTTGACC AACTCTATCA GAGCTTGGTT GACGGCAATT

     9961 TCGATGATGC AGCTTGGGCG CAGGGTCGAT GCGACGCAAT CGTCCGATCC GGAGCCGGGA

    10021 CTGTCGGGCG TACACAAATC GCCCGCAGAA GCGCGGCCGT CTGGACCGAT GGCTGTGTAG

    10081 AAGTACTCGC CGATAGTGGA AACCGACGCC CCAGCACTCG TCCGGATCGG GAGATGGGGG

    10141 AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA

    10201 AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC

    10261 CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT

    10321 TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA

    10381 CGTCGGGGCG GCAGGCCCTG CCATAGCCAC TGGCCCCGTG GGTTAGGGAC GGGGTCCCCC

    10441 ATGGGGAATG GTTTATGGTT CGTGGGGGTT ATTATTTTGG GCGTTGCGTG GGGTCAGGTC

    10501 CACGACTGGA CTGAGCAGAC AGACCCATGG TTTTTGGATG GCCTGGGCAT GGACCGCATG

    10561 TACTGGCGCG ACACGAACAC CGGGCGTCTG TGGCTGCCAA ACACCCCCGA CCCCCAAAAA

    10621 CCACCGCGCG GATTTCTGGC GTGCCAAGCT AGTCGACCAA TTCTCATGTT TGACAGCTTA

    10681 TCATCGCAGA TCCGGGCAAC GTTGTTGCCA TTGCTGCAGG CGCAGAACTG GTAGGTATGG

    10741 AAGATCTATA CATTGAATCA ATATTGGCAA TTAGCCATAT TAGTCATTGG TTATATAGCA

    10801 TAAATCAATA TTGGCTATTG GCCATTGCAT ACGTTGTATC TATATCATAA TATGTACATT

    10861 TATATTGGCT CATGTCCAAT ATGACCGCCA T

//

 

pCEP4/CAT其他相关哺乳细胞载体:

 

pEF1/V5-HisA

pEGFP-N3

pEF-GFP

pEGFP-C3

pFLAG-CMV2

pEGFP-N2

pCI-NEO

pEGFP-N1

pCEP4

pEGFP-1

pVAX1

pECFP-C1

pcDNA3.1/V5-His B

pECFP-N1

pCDNA6-Myc-HisB

pDsRed2-Nuc

pCDNA3.1-3×HA

pDsRed2-ER

pCDNA3.1-3×HA

Top-Flash

pCDNA3.1(-)/Myc-His B

pSTAT3-TA-luc

pCDNA3.1(-)/Myc-His A

pSilencer2.1-U6 neo

pCDNA3.1(+)/Myc-His B

psiCHECK-2

pCDNA3.1(+)-Zeo

pRL-TK

pCDNA3.1(+)-EGFP

pRL-SV40

pCDNA3.1(+)

pRL-CMV

pCDNA3.1(-)

pp53-TA-luc

pBudCE4.1-hrGFP

pNF-KB-TA-LUC

pCAGGS-HA

pNF-kB-Luc

pVAX1-FLAG

pMIR-Reporter

pSV2-DHFR

pmirGLO

pCEP4/CAT

pGL4.79[hRluc/Neo]

pCAGGS

pGL4.75[hRluc/CMV]

pCAG-GFP

pGL4.73[hRluc /SV40]

pCDNA3.0

pGL4.29[luc2P/CRE/Hygro]

pCDNA3.0-HA

pGL4.13-luci2

pCDNA3.1(+)-3×FLAG

pGL4.13-luci2

pSV-Beta-Gal

pGL4.10[luc2]

SuperCos1

pGL3-Enhancer

pT2/VENUS

pGL3-Control

pAdVAntage

pGL3-Basic

pEYFP-Tubulin

pGL3-3.6kb human IFN-gamma luc

pEGFP-C1

pENTR5'-UbCp

pmGeos-M-N1

pBiFC-VN173

pEYFP-N1

pBiFC-VC155

pEYFP-C1

pBIFC-bFosVC155

pEGFP-C2

pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP)   VC155

pEYFP-Actin

Laconic/pcDNA3.1(-)

pDsRed2-Peroxi

Fop-Flash

pEBFP-C1

pTet-On-Advanced

pmGeos-M-C1

pmCherry-EGFP-LC3b-r

pCerulean-N1

pmCherry-EGFP-LC3b-m

pmKO2-N1

pmCherry-EGFP-LC3B-h

pMyosin-PSmOrange

pEGFP-LC3B-r

pTagBFP-N

pEGFP-LC3B-m

pCAG-DsRed

pEGFP-LC3B-h

pmCherry-C1

pEGFP-LC3B-c

pmCherry-N1

Super PiggyBac Transposase (PB200PA-1)

pEYFP-Golgi

pTol2-Promoter-T2A-EGFP

pEYFP-Mem

pSP64-Tol2-Transposase

pDsRed2-C1

PiggyBac Dual promoter (PB513B-1)

pEGFP-Actin

pBIND-ID

pEBFP-N1

pG5Luc

pDsRed2-N1

pFUSE-hIgG1e1-Fc2

pN3

pEE12.4

pEGFP-EcoRV

pEE6.4