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pFastBacHTA

pFastBacHTA

pFastBacHTA

pFastBacHTA

 



编号

载体名称

北京华越洋VECT75433

pFastBacHTA

 

pFastBacHTA载体基本信息:

复制子:

pUC ori,F1 ori

终止子:

SV40 poly(A) signal

质粒分类:

广宿主系列,昆虫细胞载体

质粒大小:

4856bp

原核抗性:

Amp

筛选标记:

Gen

克隆菌株:

DH5α

培养条件:

37℃,有氧 LB

表达宿主:

昆虫细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:

pFastbac-F: TATTCCGGATTATTCATACC

3'测序引物:

pFastBac-R: ACAAATGTGGTATGGCTGA

 

pFastBacHTA载体图谱:


pFastBacHTA载体简介:

 Bac-to-BacHT Vector Kit 设计用于在大肠杆菌 (E. coli) 中生成杆状病毒载体后,在 Sf9、Sf21 或 High Five 细胞中表达和纯化带组氨酸标签的重组蛋白。 pFastBacHT 载体具有以下特点:1. 用于表达蛋白质的多角体强启动子 2. 用于简化克隆的三个读码框 3.用于轻松纯化重组融合蛋白的 N-端 6xHis 标签 4.用于在蛋白质纯化后去除组氨酸标签的 TEV 蛋白酶切割位点

 

pFastBacHTA载体序列:

ORIGIN

        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc

       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc

      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt

      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg

      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt

      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta

      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt

      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat

      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg

      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa

      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac

      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac

      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt

      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc

      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca

      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc

      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag

     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa

     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg

     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa

     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg

     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt

     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt

     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag

     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat

     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct

     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct

     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca

     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc

     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc

     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct

     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag

     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc

     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg

     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag

     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt

     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac

     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg

     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc

     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg

     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct

     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga

     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag

     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt

     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga

     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac

     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg

     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg

     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca

     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact

     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc

     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta

     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct

     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg

     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg

     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca

     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa

     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa

     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca

     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac

     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc

     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg

     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt

     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt

     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa

     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt

     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca

     4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg

     4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggat ccggaattca

     4141 aaggcctacg tcgacgagct caactagtgc ggccgctttc gaatctagag cctgcagtct

     4201 cgaggcatgc ggtaccaagc ttgtcgagaa gtactagagg atcataatca gccataccac

     4261 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca

     4321 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata

     4381 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg

     4441 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag

     4501 gagatccgaa ccagataagt gaaatctagt tccaaactat tttgtcattt ttaattttcg

     4561 tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc ctaaataatc

     4621 cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc cacagcgggg

     4681 catttttctt cctgttatgt ttttaatcaa acatcctgcc aactccatgt gacaaaccgt

     4741 catcttcggc tactttttct ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat

     4801 taatgtttgt aattgactga atatcaacgc ttatttgcag cctgaatggc gaatgg

//

 

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