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首页    载体及质粒    pSilencer2.1-U6 neo
pSilencer2.1-U6neo

pSilencer2.1-U6 neo

pSilencer2.1-U6 neo

pSilencer2.1-U6 neo

 



编号

载体名称

北京华越洋VECT90314

pSilencer2.1-U6 neo

 

pSilencer2.1-U6 neo载体基本信息:

启动子:

SV40,U6

复制子:

pUC ori

终止子:

SV40 poly(A) signal

质粒分类:

哺乳细胞,信号通路报告载体

质粒大小:

4521bp

原核抗性:

Amp

筛选标记:

Neo

克隆菌株:

DH5α

培养条件:

37℃,有氧 LB

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:

U6:ATGGACTATCATATGCTTACCGTA

3'测序引物:

根据序列设计引物

 

pSilencer2.1-U6 neo载体质粒图谱和多克隆位点信息:

pSilencer2.1-U6 neo载体简介:

       This Ambion vector is for the expression of siRNA. It has an antibiotic resistance gene (neomycin) to enable selection of transfected cells and features the human RNA polymerase III promoter (U6). Sufficient reagents are provided for 20 reactions.

 

pSilencer2.1-U6 neo载体序列

pSilencer2.1-U6 neo其他相关信号通路报告载体:

pEF1/V5-HisA

pEGFP-N3

pEF-GFP

pEGFP-C3

pFLAG-CMV2

pEGFP-N2

pCI-NEO

pEGFP-N1

pCEP4

pEGFP-1

pVAX1

pECFP-C1

pcDNA3.1/V5-His B

pECFP-N1

pCDNA6-Myc-HisB

pDsRed2-Nuc

pCDNA3.1-3×HA

pDsRed2-ER

pCDNA3.1-3×HA

Top-Flash

pCDNA3.1(-)/Myc-His B

pSTAT3-TA-luc

pCDNA3.1(-)/Myc-His A

pSilencer2.1-U6 neo

pCDNA3.1(+)/Myc-His B

psiCHECK-2

pCDNA3.1(+)-Zeo

pRL-TK

pCDNA3.1(+)-EGFP

pRL-SV40

pCDNA3.1(+)

pRL-CMV

pCDNA3.1(-)

pp53-TA-luc

pBudCE4.1-hrGFP

pNF-KB-TA-LUC

pCAGGS-HA

pNF-kB-Luc

pVAX1-FLAG

pMIR-Reporter

pSV2-DHFR

pmirGLO

pCEP4/CAT

pGL4.79[hRluc/Neo]

pCAGGS

pGL4.75[hRluc/CMV]

pCAG-GFP

pGL4.73[hRluc /SV40]

pCDNA3.0

pGL4.29[luc2P/CRE/Hygro]

pCDNA3.0-HA

pGL4.13-luci2

pCDNA3.1(+)-3×FLAG

pGL4.13-luci2

pSV-Beta-Gal

pGL4.10[luc2]

SuperCos1

pGL3-Enhancer

pT2/VENUS

pGL3-Control

pAdVAntage

pGL3-Basic

pEYFP-Tubulin

pGL3-3.6kb human IFN-gamma luc

pEGFP-C1

pENTR5'-UbCp

pmGeos-M-N1

pBiFC-VN173

pEYFP-N1

pBiFC-VC155

pEYFP-C1

pBIFC-bFosVC155

pEGFP-C2

pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP)   VC155

pEYFP-Actin

Laconic/pcDNA3.1(-)

pDsRed2-Peroxi

Fop-Flash

pEBFP-C1

pTet-On-Advanced

pmGeos-M-C1

pmCherry-EGFP-LC3b-r

pCerulean-N1

pmCherry-EGFP-LC3b-m

pmKO2-N1

pmCherry-EGFP-LC3B-h

pMyosin-PSmOrange

pEGFP-LC3B-r

pTagBFP-N

pEGFP-LC3B-m

pCAG-DsRed

pEGFP-LC3B-h

pmCherry-C1

pEGFP-LC3B-c

pmCherry-N1

Super PiggyBac Transposase (PB200PA-1)

pEYFP-Golgi

pTol2-Promoter-T2A-EGFP

pEYFP-Mem

pSP64-Tol2-Transposase

pDsRed2-C1

PiggyBac Dual promoter (PB513B-1)

pEGFP-Actin

pBIND-ID

pEBFP-N1

pG5Luc

pDsRed2-N1

pFUSE-hIgG1e1-Fc2

pN3

pEE12.4

pEGFP-EcoRV

pEE6.4