pRL-CMV

pRL-CMV

pRL-CMV载体基本信息：

pRL-CMV载体质粒图谱和多克隆位点信息：

pRL-CMV载体简介：

        The pRL-CMV Vector may be used in combination with any experimental reporter vector to co-transfect mammalian cells. However, it is important to realize that trans effects between promoters on co-transfected plasmids can potentially affect reporter gene expression . Primarily, this is of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements (such as CMV). The occurrence and magnitude of such effects will depend on several factors: a) the combination and activities of the genetic regulatory elements present on the co-transfected vectors, b) the relative ratio of experimental vector to control vector introduced into the cells, and c) the cell type transfected.

pRL-CMV载体序列：

ORIGIN

        1 AGATCTTCAA TATTGGCCAT TAGCCATATT ATTCATTGGT TATATAGCAT AAATCAATAT

       61 TGGCTATTGG CCATTGCATA CGTTGTATCT ATATCATAAT ATGTACATTT ATATTGGCTC

      121 ATGTCCAATA TGACCGCCAT GTTGGCATTG ATTATTGACT AGTTATTAAT AGTAATCAAT

      181 TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA

      241 TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT

      301 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA

      361 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTCCGCCCC CTATTGACGT

      421 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAC GGGACTTTCC

      481 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA

      541 GTACACCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT

      601 TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA

      661 TAACCCCGCC CCGTTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG

      721 CAGAGCTCGT TTAGTGAACC GTCAGATCAC TAGAAGCTTT ATTGCGGTAG TTTATCACAG

      781 TTAAATTGCT AACGCAGTCA GTGCTTCTGA CACAACAGTC TCGAACTTAA GCTGCAGAAG

      841 TTGGTCGTGA GGCACTGGGC AGGTAAGTAT CAAGGTTACA AGACAGGTTT AAGGAGACCA

      901 ATAGAAACTG GGCTTGTCGA GACAGAGAAG ACTCTTGCGT TTCTGATAGG CACCTATTGG

      961 TCTTACTGAC ATCCACTTTG CCTTTCTCTC CACAGGTGTC CACTCCCAGT TCAATTACAG

     1021 CTCTTAAGGC TAGAGTACTT AATACGACTC ACTATAGGCT AGCCACCATG ACTTCGAAAG

     1081 TTTATGATCC AGAACAAAGG AAACGGATGA TAACTGGTCC GCAGTGGTGG GCCAGATGTA

     1141 AACAAATGAA TGTTCTTGAT TCATTTATTA ATTATTATGA TTCAGAAAAA CATGCAGAAA

     1201 ATGCTGTTAT TTTTTTACAT GGTAACGCGG CCTCTTCTTA TTTATGGCGA CATGTTGTGC

     1261 CACATATTGA GCCAGTAGCG CGGTGTATTA TACCAGACCT TATTGGTATG GGCAAATCAG

     1321 GCAAATCTGG TAATGGTTCT TATAGGTTAC TTGATCATTA CAAATATCTT ACTGCATGGT

     1381 TTGAACTTCT TAATTTACCA AAGAAGATCA TTTTTGTCGG CCATGATTGG GGTGCTTGTT

     1441 TGGCATTTCA TTATAGCTAT GAGCATCAAG ATAAGATCAA AGCAATAGTT CACGCTGAAA

     1501 GTGTAGTAGA TGTGATTGAA TCATGGGATG AATGGCCTGA TATTGAAGAA GATATTGCGT

     1561 TGATCAAATC TGAAGAAGGA GAAAAAATGG TTTTGGAGAA TAACTTCTTC GTGGAAACCA

     1621 TGTTGCCATC AAAAATCATG AGAAAGTTAG AACCAGAAGA ATTTGCAGCA TATCTTGAAC

     1681 CATTCAAAGA GAAAGGTGAA GTTCGTCGTC CAACATTATC ATGGCCTCGT GAAATCCCGT

     1741 TAGTAAAAGG TGGTAAACCT GACGTTGTAC AAATTGTTAG GAATTATAAT GCTTATCTAC

     1801 GTGCAAGTGA TGATTTACCA AAAATGTTTA TTGAATCGGA CCCAGGATTC TTTTCCAATG

     1861 CTATTGTTGA AGGTGCCAAG AAGTTTCCTA ATACTGAATT TGTCAAAGTA AAAGGTCTTC

     1921 ATTTTTCGCA AGAAGATGCA CCTGATGAAA TGGGAAAATA TATCAAATCG TTCGTTGAGC

     1981 GAGTTCTCAA AAATGAACAA TAATTCTAGA GCGGCCGCTT CGAGCAGACA TGATAAGATA

     2041 CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA

     2101 AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA

     2161 CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG

     2221 CAAGTAAAAC CTCTACAAAT GTGGTAAAAT CGATAAGGAT CCAGGTGGCA CTTTTCGGGG

     2281 AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT

     2341 CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT

     2401 TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC

     2461 TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG

     2521 TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG

     2581 TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA

     2641 CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA

     2701 CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC

     2761 TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC

     2821 GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG

     2881 GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC

     2941 AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA

     3001 ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT

     3061 TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT

     3121 CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG

     3181 GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT

     3241 TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT

     3301 TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT

     3361 CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC

     3421 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT

     3481 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG

     3541 CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA

     3601 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC

     3661 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA

     3721 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC

     3781 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA

     3841 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG

     3901 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG

     3961 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG

     4021 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGGCTCGAC

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pRL-CMV其他相关信号通路报告载体：