pPD95_67
pPD95_67
编号 | 载体名称 |
北京华越洋VECT75472 | pPD95_67 |
pPD95_67载体基本信息：
启动子: | Lac/lac |
复制子: | pUC ori |
质粒分类: | 线虫表达质粒 |
质粒大小: | 4532bp |
质粒标签: | IVSA, SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL, decoy |
原核抗性: | 氨苄青霉素Ampicillin |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | 线虫 |
诱导方式: | 参考相关文献 |
5'测序引物: | M13R:CAGGAAACAGCTATGACC |
3'测序引物: | 根据全序列设计 |
pPD95_67载体图谱：
pPD95_67载体简介：
pPD95_67 是一个线虫表达质粒，有GFP标签。
pPD95_67载体序列：
ORIGIN
1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
121 accgagctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg tagaaaaaat
181 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
241 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
301 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggg taagtttaaa
361 catatatata ctaactaacc ctgattattt aaattttcag ccaacacttg tcactacttt
421 ctgttatggt gttcaatgct tctcgagata cccagatcat atgaaacggc atgacttttt
481 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg
541 gaactacaag acacgtaagt ttaaacagtt cggtactaac taaccataca tatttaaatt
601 ttcaggtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
661 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
721 acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagttg taagtttaaa
781 catgatttta ctaactaact aatctgattt aaattttcag aacttcaaaa ttagacacaa
841 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
901 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
961 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
1021 acatggcatg gatgaactat acaaatagca ttcgtagaat tccaactgag cgccggtcgc
1081 taccattacc aacttgtctg gtgtcaaaaa taataggggc cgctgtcatc agagtaagtt
1141 taaactgagt tctactaact aacgagtaat atttaaattt tcagcatctc gcgcccgtgc
1201 ctctgacttc taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
1261 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
1321 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
1381 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
1441 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
1501 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcaa
1561 tttttatttc ttcgcacgtc tgggcctctc atgacgtcaa atcatgctca tcgtgaaaaa
1621 gttttggagt atttttggaa tttttcaatc aagtgaaagt ttatgaaatt aattttcctg
1681 cttttgcttt ttggggtttc ccctattgtt tgtcaagatt tcgaggacgg cgtttttctt
1741 gctaaaatca caagtattga tgagcacgat gcaagaaaga tcggaagaag gtttgggttt
1801 gaggctcagt ggaaggtgag tagaagttga taatttgaaa gtggagtagt gtctatgggg
1861 tttttgcctt aaatgacaga atacattccc aatataccaa acataactgt ttcctactag
1921 tcggccgtac gggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
1981 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
2041 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
2101 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
2161 gagaaaatac cgcatcaggc ggccttaagg gcctcgtgat acgcctattt ttataggtta
2221 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
2281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
2341 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
2401 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
2461 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
2521 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
2581 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
2641 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
2701 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
2761 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
2821 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
2881 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
2941 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
3001 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
3061 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
3121 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
3181 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
3241 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
3301 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
3361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
3421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
3481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
3541 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
3601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
3661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
3721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
3781 aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg
3841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
3901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
3961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
4021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
4081 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
4141 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
4201 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
4261 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
4321 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
4381 ttcacacagg aaacagctat gaccatgatt acgccaagct gtaagtttaa acatgatctt
4441 actaactaac tattctcatt taaattttca gagcttaaaa atggctgaaa tcactcacaa
4501 cgatggatac gctaacaact tggaaatgaa at
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pPD95_67载体其他相关的线虫系列载体：
