pNF-kB-Luc
pNF-kB-Luc
编号 | 载体名称 |
北京华越洋VECT90296 | pNF-kB-Luc |
pNF-kB-Luc载体基本信息：
启动子: | SV40 |
复制子: | pUC ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞，信号通路报告载体 |
质粒大小: | 5610bp |
原核抗性: | Amp |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导，瞬时表达 |
5'测序引物: | Sv40-polyA-R：GAAATTTGTGATGCTATTGC |
3'测序引物: | 根据序列设计引物 |
pNF-kB-Luc载体质粒图谱和多克隆位点信息：
pNF-kB-Luc载体简介：
PNF nuclear factor kappa B-luc can be transfected into cells by conventional cell transfection method. The detection can be used when the luciferase reporter gene biyuntian detection kit (RG005/RG006) or dual luciferase reporter gene assay kit (RG008/RG009).
TNF- alpha, IL-1 beta and LPS are common reagents that can activate the NF nuclear factor kappa B and can be used as a positive control for the detection of pNF nuclear factor kappa B-luc reporter genes.
pNF-kB-Luc载体序列
pNF-kB-Luc其他相关信号通路报告载体：
