pMIR-Reporter

pMIR-Reporter

pMIR-Reporter载体基本信息：

pMIR-Reporter载体质粒图谱和多克隆位点信息：

pMIR-Reporter载体简介：

       The pMIR-REPORT™ miRNA Expression Reporter Vector System consists of an experimental firefly luciferase reporter vector and an associated β-gal reporter control plasmid. By inserting predicted miRNA target sequences in the multiple cloning site, pMIR-REPORT Luciferase miRNA Expression Reporter Vector can be used to conduct accurate, quantitative evaluations of miRNA function. It can also be used to evaluate siRNA target sites and to analyze the influence of 3' UTR sequences on gene expression. pMIR-REPORT Luciferase contains a firefly luciferase reporter gene under the control of a CMV promoter/termination system. The 3' UTR of the luciferase gene contains a multiple cloning site for insertion of predicted miRNA binding targets or other nucleotide sequences. By cloning a predicted miRNA target sequence into pMIR-REPORT, the luciferase reporter is subjected to regulation that mimics the miRNA target.

pMIR-Reporter载体序列：

ORIGIN

        1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA

       61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG

      121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC

      181 ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC

      241 ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT

      301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT

      361 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTAGCGGCC GCATACAAAA

      421 AACCAACACA CAGATCCAAT GAAAATAAAA GATCCTTTAT TAAGCTTAAC TGTTTAAACC

      481 TATGATATCA CGCACGCGTC TATGCCGGCC TATGAGCTCC CTCACTAGTG CGCAGCTTTC

      541 ATCACTCGAG CAATTTGGAC TTTCCGCCCT TCTTGGCCTT TATGAGGATC TCTCTGATTT

      601 TTCTTGCGTC GAGTTTTCCG GTAAGACCTT TCGGTACTTC GTCCACAAAC ACAACTCCTC

      661 CGCGCAACTT TTTCGCGGTT GTTACTTGAC TGGCGACGTA ATCCACGATC TCTTTTTCCG

      721 TCATCGTCTT TCCGTGCTCC AAAACAACAA CGGCGGCGGG AAGTTCACCG GCGTCATCGT

      781 CGGGAAGACC TGCCACGCCC GCGTCGAAGA TGTTGGGGTG TTGTAACAAT ATCGATTCCA

      841 ATTCAGCGGG GGCCACCTGA TATCCTTTGT ATTTAATTAA AGACTTCAAG CGGTCAACTA

      901 TGAAGAAGTG TTCGTCTTCG TCCCAGTAAG CTATGTCTCC AGAATGTAGC CATCCATCCT

      961 TGTCAATCAA GGCGTTGGTC GCTTCCGGAT TGTTTACATA ACCGGACATA ATCATAGGTC

     1021 CTCTGACACA TAATTCGCCT CTCTGATTAA CGCCCAGCGT TTTCCCGGTA TCCAGATCCA

     1081 CAACCTTCGC TTCAAAAAAT GGAACAACTT TACCGACCGC GCCCGGTTTA TCATCCCCCT

     1141 CGGGTGTAAT CAGAATAGCT GATGTAGTCT CAGTGAGCCC ATATCCTTGT CGTATCCCTG

     1201 GAAGATGGAA GCGTTTTGCA ACCGCTTCCC CGACTTCTTT CGAAAGAGGT GCGCCCCCAG

     1261 AAGCAATTTC GTGTAAATTA GATAAATCGT ATTTGTCAAT CAGAGTGCTT TTGGCGAAGA

     1321 ATGAAAATAG GGTTGGTACT AGCAACGCAC TTTGAATTTT GTAATCCTGA AGGGATCGTA

     1381 AAAACAGCTC TTCTTCAAAT CTATACATTA AGACGACTCG AAATCCACAT ATCAAATATC

     1441 CGAGTGTAGT AAACATTCCA AAACCGTGAT GGAATGGGAC AACACTTAAA ATCGCAGTAT

     1501 CCGGAACGAT TTGATTGCCA AAAATAGGAT CTCTGGCATG CGAGAATCTG ACGCAGGCAG

     1561 TTCTATGCGG AAGGGCCACA CCCTTAGGTA ACCCAGTAGA TCCAGAGGAA TTCATTATCA

     1621 GTGCAATTGT TTTGTCACGA TCAAAGGACT CTGGTACAAA ATCGTATTCA TTAAAACCGG

     1681 GAGGTAGATG AGATGTGACG AACGTGTACA TCGACTGAAA TCCCTGGTAA TCCGTTTTAG

     1741 AATCCATGAT AATAATTTTC TGGATTATTG GTAATTTTTT TTGCACGTTC AAAATTTTTT

     1801 GCAACCCCTT TTTGGAAACA AACACTACGG TAGGCTGCGA AATGTTCATA CTGTTGAGCA

     1861 ATTCACGTTC ATTATAAATG TCGTTCGCGG GCGCAACTGC AACTCCGATA AATAACGCGC

     1921 CCAACACCGG CATAAAGAAT TGAAGAGAGT TTTCACTGCA TACGACGATT CTGTGATTTG

     1981 TATTCAGCCC ATATCGTTTC ATAGCTTCTG CCAACCGAAC GGACATTTCG AAGTATTCCG

     2041 CGTACGTGAT GTTCACCTCG ATATGTGCAT CTGTAAAAGC AATTGTTCCA GGAACCAGGG

     2101 CGTATCTCTT CATAGCCTTA TGCAGTTGCT CTCCAGCGGT TCCATCCTCT AGAGGATAGA

     2161 ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT GGTGGATCCA CGGTTCACTA

     2221 AACCAGCTCT GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG

     2281 GGGCGGAGTT GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC

     2341 CATTGACGTC AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC

     2401 CATTGATGTA CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT

     2461 ACTGCCAAGT AGGAAAGTCC CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT

     2521 TACCGTCATT GACGTCAATA GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA

     2581 AGTGGGCAGT TTACCGTAAA TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG

     2641 TTACTATGGG AACATACGTC ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC

     2701 CAGGCGGGCC ATTTACCGTA AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA

     2761 ATGACCCCGT AATTGATTAC TATTAATAAC TAAGATCTGG TACCTTGAAT TCATGCTTCT

     2821 CCTCCCTTTA GTGAGGGTAA TTCTCTCTCT CTCCCTATAG TGAGTCGTAT TAATTCCTTC

     2881 TCTTCTATAG TGTCACCTAA ATCGTTGCAA TTCGTAATCA TGTCATAGCT GTTTCCTGTG

     2941 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA

     3001 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT

     3061 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA

     3121 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC

     3181 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA

     3241 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT

     3301 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA

     3361 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT

     3421 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG

     3481 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC

     3541 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC

     3601 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA

     3661 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT

     3721 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC

     3781 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA

     3841 AAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA

     3901 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA

     3961 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT

     4021 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC

     4081 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC

     4141 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC

     4201 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA

     4261 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC

     4321 CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC

     4381 AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA

     4441 TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA

     4501 GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA

     4561 CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT

     4621 TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT

     4681 TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG

     4741 CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA

     4801 TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC

     4861 AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG

     4921 ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG

     4981 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG

     5041 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT TATTATCATG

     5101 ACATTAACCT ATAAAAATAG GCGTATCACG AGATTGCAGT GAAAAAAATG CTTTATTTGT

     5161 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC

     5221 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA

     5281 AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCCTCTAGA GTCGGTGGGC

     5341 CTCGGGGGCG GGTGCGGGGT CGGCGGGGCC GCCCCGGGTG GCTTCGGTCG GAGCCATGGG

     5401 GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG CGGGCGTCAG GCACCGGGCT

     5461 TGCGGGTCAT GCACCAGGTG CGCGGTCCTT CGGGCACCTC GACGTCGGCG GTGACGGTGA

     5521 AGCCGAGCCG CTCGTAGAAG GGGAGGTTGC GGGGCGCGGA GGTCTCCAGG AAGGCGGGCA

     5581 CCCCGGCGCG CTCGGCCGCC TCCACTCCGG GGAGCACGAC GGCGCTGCCC AGACCCTTGC

     5641 CCTGGTGGTC GGGCGAGACG CCGACGGTGG CCAGGAACCA CGCGGGCTCC TTGGGCCGGT

     5701 GCGGCGCCAG GAGGCCTTCC ATCTGTTGCT GCGCGGCCAG CCGGGAACCG CTCAACTCGG

     5761 CCATGCGCGG GCCGATCTCG GCGAACACCG CCCCCGCTTC GACGCTCTCC GGCGTGGTCC

     5821 AGACCGCCAC CGCGGCGCCG TCGTCCGCGA CCCACACCTT GCCGATGTCG AGCCCGACGC

     5881 GCGTGAGGAA GAGTTCTTGC AGCTCGGTGA CCCGCTCGAT GTGGCGGTCC GGGTCGACGG

     5941 TGTGGCGCGT GGCGGGGTAG TCGGCGAACG CGGCGGCGAG GGTGCGTACG GCCCGGGGGA

     6001 CGTCGTCGCG GGTGGCGAGG CGCACCGTGG GCTTGTACTC GGTCATGGAA GGTCGTCTCC

     6061 TTGTGAGGGG TCAGGGGCGT GGGTCAGGGG ATGGTGGCGG CACCGGTCGT GGCGGCCGAC

     6121 CTGCAGGCAT GCAAGCTAGC TTTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT

     6181 ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA

     6241 GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG

     6301 TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG

     6361 CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA

     6421 TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCA ATCTTTCGTC

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pMIR-Reporter其他相关信号通路报告载体：