北京华越洋生物
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首页    载体及质粒    pGL4.79[hRluc/Neo]
pGL4.79-hRluc-Neo-

pGL4.79[hRluc/Neo]

pGL4.79[hRluc/Neo]

pGL4.79[hRluc/Neo]

 



编号

载体名称

北京华越洋VECT90304

pGL4.79[hRluc/Neo]

 

pGL4.79[hRluc/Neo]载体基本信息:

启动子:

SV40

复制子:

pUC oriSV40 ori

终止子:

SV40 poly(A) signal

质粒分类:

哺乳细胞,信号通路报告载体

质粒大小:

4879bp

原核抗性:

Amp

筛选标记:

Neo

克隆菌株:

DH5α

培养条件:

37℃,有氧 LB

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:

RVP3:CTAGCAAAATAGGCTGTCCC

3'测序引物:

根据序列设计引物

 

pGL4.79[hRluc/Neo]载体质粒图谱和多克隆位点信息:

 

 

pGL4.79[hRluc/Neo]载体简介:

       The pGL4.79[hRluc/Neo] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for neomycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.79[hRluc/Neo] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.

 

pGL4.79[hRluc/Neo]载体序列:

ORIGIN

        1 GGCCTAACTG GCCGGTACCT GAGCTCGCTA GCCTCGAGGA TATCAAGATC TGGCCTCGGC

       61 GGCCAAGCTT GGCAATCCGG TACTGTTGGT AAAGCCACCA TGGCTTCCAA GGTGTACGAC

      121 CCCGAGCAAC GCAAACGCAT GATCACTGGG CCTCAGTGGT GGGCTCGCTG CAAGCAAATG

      181 AACGTGCTGG ACTCCTTCAT CAACTACTAT GATTCCGAGA AGCACGCCGA GAACGCCGTG

      241 ATTTTTCTGC ATGGTAACGC TGCCTCCAGC TACCTGTGGA GGCACGTCGT GCCTCACATC

      301 GAGCCCGTGG CTAGATGCAT CATCCCTGAT CTGATCGGAA TGGGTAAGTC CGGCAAGAGC

      361 GGGAATGGCT CATATCGCCT CCTGGATCAC TACAAGTACC TCACCGCTTG GTTCGAGCTG

      421 CTGAACCTTC CAAAGAAAAT CATCTTTGTG GGCCACGACT GGGGGGCTTG TCTGGCCTTT

      481 CACTACTCCT ACGAGCACCA AGACAAGATC AAGGCCATCG TCCATGCTGA GAGTGTCGTG

      541 GACGTGATCG AGTCCTGGGA CGAGTGGCCT GACATCGAGG AGGATATCGC CCTGATCAAG

      601 AGCGAAGAGG GCGAGAAAAT GGTGCTTGAG AATAACTTCT TCGTCGAGAC CATGCTCCCA

      661 AGCAAGATCA TGCGGAAACT GGAGCCTGAG GAGTTCGCTG CCTACCTGGA GCCATTCAAG

      721 GAGAAGGGCG AGGTTAGACG GCCTACCCTC TCCTGGCCTC GCGAGATCCC TCTCGTTAAG

      781 GGAGGCAAGC CCGACGTCGT CCAGATTGTC CGCAACTACA ACGCCTACCT TCGGGCCAGC

      841 GACGATCTGC CTAAGATGTT CATCGAGTCC GACCCTGGGT TCTTTTCCAA CGCTATTGTC

      901 GAGGGAGCTA AGAAGTTCCC TAACACCGAG TTCGTGAAGG TGAAGGGCCT CCACTTCAGC

      961 CAGGAGGACG CTCCAGATGA AATGGGTAAG TACATCAAGA GCTTCGTGGA GCGCGTGCTG

     1021 AAGAACGAGC AGTAATTCTA GAGTCGGGGC GGCCGGCCGC TTCGAGCAGA CATGATAAGA

     1081 TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT

     1141 GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC

     1201 AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA

     1261 AGCAAGTAAA ACCTCTACAA ATGTGGTAAA ATCGATAAGG ATCCGTTTGC GTATTGGGCG

     1321 CTCTTCCGCT GATCTGCGCA GCACCATGGC CTGAAATAAC CTCTGAAAGA GGAACTTGGT

     1381 TAGCTACCTT CTGAGGCGGA AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA

     1441 AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA

     1501 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA

     1561 ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA

     1621 GTTCCGCCCA TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG

     1681 CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT

     1741 TTTGCAAAAA GCTCGATTCT TCTGACACTA GCGCCACCAT GATCGAACAA GACGGCCTCC

     1801 ATGCTGGCAG TCCCGCAGCT TGGGTCGAAC GCTTGTTCGG GTACGACTGG GCCCAGCAGA

     1861 CCATCGGATG TAGCGATGCG GCCGTGTTCC GTCTAAGCGC TCAAGGCCGG CCCGTGCTGT

     1921 TCGTGAAGAC CGACCTGAGC GGCGCCCTGA ACGAGCTTCA AGACGAGGCT GCCCGCCTGA

     1981 GCTGGCTGGC CACCACCGGC GTACCCTGCG CCGCTGTGTT GGATGTTGTG ACCGAAGCCG

     2041 GCCGGGACTG GCTGCTGCTG GGCGAGGTCC CTGGCCAGGA TCTGCTGAGC AGCCACCTTG

     2101 CCCCCGCTGA GAAGGTTTCT ATCATGGCCG ATGCAATGCG GCGCCTGCAC ACCCTGGACC

     2161 CCGCTACCTG CCCCTTCGAC CACCAGGCTA AGCATCGGAT CGAGCGTGCT CGGACCCGCA

     2221 TGGAGGCCGG CCTGGTGGAC CAGGACGACC TGGACGAGGA GCATCAGGGC CTGGCCCCCG

     2281 CTGAACTGTT CGCCCGACTG AAAGCCCGCA TGCCGGACGG TGAGGACCTG GTTGTCACAC

     2341 ACGGAGATGC CTGCCTCCCT AACATCATGG TCGAGAATGG CCGCTTCTCC GGCTTCATCG

     2401 ACTGCGGTCG CCTAGGAGTT GCCGACCGCT ACCAGGACAT CGCCCTGGCC ACCCGCGACA

     2461 TCGCTGAGGA GCTTGGCGGC GAGTGGGCCG ACCGCTTCTT AGTCTTGTAC GGCATCGCAG

     2521 CTCCCGACAG CCAGCGCATC GCCTTCTACC GCTTGCTCGA CGAGTTCTTT TAATGATCTA

     2581 GAACCGGTCA TGGCCGCAAT AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT

     2641 TGTGTGTTCG AACTAGATGC TGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT

     2701 CCTTCCGGTG GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA

     2761 TGCAACTCGT AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG

     2821 CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA

     2881 TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC

     2941 AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG

     3001 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC

     3061 CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC

     3121 GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT

     3181 AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC

     3241 GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA

     3301 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA

     3361 GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA

     3421 TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA

     3481 TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG

     3541 CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG

     3601 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC

     3661 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT

     3721 TGGTCTGACA GCGGCCGCAA ATGCTAAACC ACTGCAGTGG TTACCAGTGC TTGATCAGTG

     3781 AGGCACCGAT CTCAGCGATC TGCCTATTTC GTTCGTCCAT AGTGGCCTGA CTCCCCGTCG

     3841 TGTAGATCAC TACGATTCGT GAGGGCTTAC CATCAGGCCC CAGCGCAGCA ATGATGCCGC

     3901 GAGAGCCGCG TTCACCGGCC CCCGATTTGT CAGCAATGAA CCAGCCAGCA GGGAGGGCCG

     3961 AGCGAAGAAG TGGTCCTGCT ACTTTGTCCG CCTCCATCCA GTCTATGAGC TGCTGTCGTG

     4021 ATGCTAGAGT AAGAAGTTCG CCAGTGAGTA GTTTCCGAAG AGTTGTGGCC ATTGCTACTG

     4081 GCATCGTGGT ATCACGCTCG TCGTTCGGTA TGGCTTCGTT CAACTCTGGT TCCCAGCGGT

     4141 CAAGCCGGGT CACATGATCA CCCATATTAT GAAGAAATGC AGTCAGCTCC TTAGGGCCTC

     4201 CGATCGTTGT CAGAAGTAAG TTGGCCGCGG TGTTGTCGCT CATGGTAATG GCAGCACTAC

     4261 ACAATTCTCT TACCGTCATG CCATCCGTAA GATGCTTTTC CGTGACCGGC GAGTACTCAA

     4321 CCAAGTCGTT TTGTGAGTAG TGTATACGGC GACCAAGCTG CTCTTGCCCG GCGTCTATAC

     4381 GGGACAACAC CGCGCCACAT AGCAGTACTT TGAAAGTGCT CATCATCGGG AATCGTTCTT

     4441 CGGGGCGGAA AGACTCAAGG ATCTTGCCGC TATTGAGATC CAGTTCGATA TAGCCCACTC

     4501 TTGCACCCAG TTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCGGGG TGTGCAAAAA

     4561 CAGGCAAGCA AAATGCCGCA AAGAAGGGAA TGAGTGCGAC ACGAAAATGT TGGATGCTCA

     4621 TACTCGTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTACTAGTAC GTCTCTCAAG

     4681 GATAAGTAAG TAATATTAAG GTACGGGAGG TATTGGACAG GCCGCAATAA AATATCTTTA

     4741 TTTTCATTAC ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC ATACGCTCTC

     4801 CATCAAAACA AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA

     4861 GGTGCCAGAA CATTTCTCT

//

 

pGL4.79[hRluc/Neo]其他相关信号通路报告载体:

pEF1/V5-HisA

pEGFP-N3

pEF-GFP

pEGFP-C3

pFLAG-CMV2

pEGFP-N2

pCI-NEO

pEGFP-N1

pCEP4

pEGFP-1

pVAX1

pECFP-C1

pcDNA3.1/V5-His B

pECFP-N1

pCDNA6-Myc-HisB

pDsRed2-Nuc

pCDNA3.1-3×HA

pDsRed2-ER

pCDNA3.1-3×HA

Top-Flash

pCDNA3.1(-)/Myc-His B

pSTAT3-TA-luc

pCDNA3.1(-)/Myc-His A

pSilencer2.1-U6 neo

pCDNA3.1(+)/Myc-His B

psiCHECK-2

pCDNA3.1(+)-Zeo

pRL-TK

pCDNA3.1(+)-EGFP

pRL-SV40

pCDNA3.1(+)

pRL-CMV

pCDNA3.1(-)

pp53-TA-luc

pBudCE4.1-hrGFP

pNF-KB-TA-LUC

pCAGGS-HA

pNF-kB-Luc

pVAX1-FLAG

pMIR-Reporter

pSV2-DHFR

pmirGLO

pCEP4/CAT

pGL4.79[hRluc/Neo]

pCAGGS

pGL4.75[hRluc/CMV]

pCAG-GFP

pGL4.73[hRluc /SV40]

pCDNA3.0

pGL4.29[luc2P/CRE/Hygro]

pCDNA3.0-HA

pGL4.13-luci2

pCDNA3.1(+)-3×FLAG

pGL4.13-luci2

pSV-Beta-Gal

pGL4.10[luc2]

SuperCos1

pGL3-Enhancer

pT2/VENUS

pGL3-Control

pAdVAntage

pGL3-Basic

pEYFP-Tubulin

pGL3-3.6kb human IFN-gamma luc

pEGFP-C1

pENTR5'-UbCp

pmGeos-M-N1

pBiFC-VN173

pEYFP-N1

pBiFC-VC155

pEYFP-C1

pBIFC-bFosVC155

pEGFP-C2

pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP)   VC155

pEYFP-Actin

Laconic/pcDNA3.1(-)

pDsRed2-Peroxi

Fop-Flash

pEBFP-C1

pTet-On-Advanced

pmGeos-M-C1

pmCherry-EGFP-LC3b-r

pCerulean-N1

pmCherry-EGFP-LC3b-m

pmKO2-N1

pmCherry-EGFP-LC3B-h

pMyosin-PSmOrange

pEGFP-LC3B-r

pTagBFP-N

pEGFP-LC3B-m

pCAG-DsRed

pEGFP-LC3B-h

pmCherry-C1

pEGFP-LC3B-c

pmCherry-N1

Super PiggyBac Transposase (PB200PA-1)

pEYFP-Golgi

pTol2-Promoter-T2A-EGFP

pEYFP-Mem

pSP64-Tol2-Transposase

pDsRed2-C1

PiggyBac Dual promoter (PB513B-1)

pEGFP-Actin

pBIND-ID

pEBFP-N1

pG5Luc

pDsRed2-N1

pFUSE-hIgG1e1-Fc2

pN3

pEE12.4

pEGFP-EcoRV

pEE6.4