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pSilent-Dual1

pSilent-Dual1

pSilent-Dual1

pSilent-Dual1

 



编号

载体名称

北京华越洋VECT75461

pSilent-Dual1

 

pSilent-Dual1载体基本信息:

启动子:

PgpdA, TrpC

复制子:

pUC ori,F1 ori

质粒分类:

丝状真菌载体;RNAi基因沉默干扰质粒

质粒大小:

5567bp

原核抗性:

氨苄青霉素Ampicillin

筛选标记:

新霉素Neomycin/G418

克隆菌株:

DH5α

培养条件:

37℃,有氧,LB

表达宿主:

丝状真菌

5'测序引物:

根据序列设计

3'测序引物:

根据序列设计

 

pSilent-Dual1载体图谱:

pSilent-Dual1载体简介:

an RNA-silencing vector, pSilent-Dual1 (pSD1), with a convergent dual promoter system that provides a high-throughput platform for functional genomics research in filamentous fungi. In the pSD1 system.

 

pSilent-Dual1载体序列:

ORIGIN

        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc

       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga

      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc

      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc

      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag

      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa

      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac

      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg

      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg

      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg

      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg

      661 gccccccctc gaggacgtta actgatattg aaggagcact ttttgggctt ggctggagct

      721 agtggaggtc aacaatgaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat

      781 ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc

      841 agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat

      901 catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtgaacgacc

      961 cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc

     1021 tattctaccc aagcatcgat atgattgaac aagatggatt gcacgcaggt tctccggccg

     1081 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg

     1141 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt

     1201 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg

     1261 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat

     1321 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat

     1381 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg

     1441 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg

     1501 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc

     1561 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc

     1621 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg

     1681 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg

     1741 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca

     1801 tcgccttcta tcgccttctt gacgagttct tctgaggatc cacttaacgt tactgaaatc

     1861 atcaaacagc ttgacgaatc tggatataag atcgttggtg tcgatgtcag ctccggagtt

     1921 gagacaaatg gtgttcagga tctcgataag atacgttcat ttgtccaagc agcaaagagt

     1981 gccttctagt gatttaatag ctccatgtca acaagaataa aacgcgtttc gggtttacct

     2041 cttccagata cagctcatct gcaatgcatt aatgcattgg acctcgcaac cctagtacgc

     2101 ccttcaggct ccggcgaagc agaagaatag cttagcagag tctattttca ttttcgggag

     2161 acgagatcaa gcagatcaac ggtcgtcaag agacctacga gactgaggaa tccgctcttg

     2221 gctccacgcg actatatatt tgtctctaat tgtactttga catgctcctc ttctttactc

     2281 tgatagcttg actatgaaaa ttccgtcacc agccctgggt tctcgaggtc gacgacgtta

     2341 actgagattg aaggagcact ttttgggctt ggctggagct agtggaggtc aacaatgaat

     2401 gcctattttg gtttagtcgt ccaggcggtg agcacaaaat ttgtgtcgtt tgacaagatg

     2461 gttcatttag gcaactggtc agatcagccc cacttgtagc agtagcggcg gcgctcgaag

     2521 tgtgactctt attagcagac aggaacgagg acattattat catctgctgc ttggtgcacg

     2581 ataacttggt gcgtttgtca agcaaggtaa gtgaacgacc cggtcatacc ttcttaagtt

     2641 cgcccttcct ccctttattt cagattcaat ctgacttacc tattctaccc aagcatcgat

     2701 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgcaaat

     2761 aaaggttctt ggatgggaag atgaatatac tgaagatggg aaaagaaaga gaaaagaaaa

     2821 gagcagctgg tggggagagc aggaaaatat ggcaacaaat gttggactga cgcaacgacc

     2881 ttgtcaaccc cgccgacaca ccgggcggac agacggggca aagctgccta ccagggactg

     2941 agggacctca gcaggtcgag tgcagagcac cggatgggtc gactgccagc ttgtgttccc

     3001 ggtctgcgcc gctggccagc tcctgagcgg cctttccggt ttcatacacc gggcaaagca

     3061 ggagaggcac gatatttgga cgccctacag atgccggatg ggccaattag ggagcttacg

     3121 cgccgggtac tcgctctacc tacttcggag aaggtactat ctcgtgaatc ttttaccaga

     3181 tcggaagcaa ttggacttct gtacctaggt taatggcatg ctatttcgcc gacggctata

     3241 cacccctggc ttcacattct ccttcgctta ctgccggtga ttcgatgaag ctccatattc

     3301 tccgatgatg caatagattc ttggtcaacg aggggcacac cagcctttcc acttcggggc

     3361 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc

     3421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg

     3481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt

     3541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg

     3601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga

     3661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat

     3721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca

     3781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc

     3841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata

     3901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc

     3961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc

     4021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga

     4081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc

     4141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag

     4201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag

     4261 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag

     4321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca

     4381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga

     4441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat

     4501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga

     4561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg

     4621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga

     4681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc

     4741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac

     4801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc

     4861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc

     4921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc

     4981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt

     5041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc

     5101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg

     5161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag

     5221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat

     5281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc

     5341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa

     5401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta

     5461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa

     5521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac

//

 

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