pBARGPE1-mCherry
pBARGPE1-mCherry
编号 | 载体名称 |
北京华越洋VECT75456 | pBARGPE1-mCherry |
pBARGPE1-mCherry载体基本信息：
启动子: | gpdA,TrpC |
复制子: | pUC ori |
终止子: | trpC |
质粒分类: | 其他宿主穿梭载体；丝状真菌表达质粒 |
质粒大小: | 6229bp |
质粒标签: | 单体红色荧光蛋白mCherry |
原核抗性: | 氨苄青霉素Ampicillin |
筛选标记: | 草胺膦Bar |
克隆菌株: | DH5α |
培养条件: | 37℃，有氧，LB |
表达宿主: | 丝状真菌 |
诱导方式: | 无需诱导 |
5'测序引物: | 根据全序列设计 |
3'测序引物: | 根据全序列设计 |
pBARGPE1-mCherry载体图谱：
pBARGPE1-mCherry载体简介：
pBARGPE1-mCherry是一个丝状真菌-大肠杆菌穿梭表达载体，利用gpdA promoter可启动外源目的基因的表达，转化至丝状真菌中可用草胺膦Bar筛选。
pBARGPE1-mCherry载体序列：
ORIGIN
1 gccccgtccg gtcctgcccg tcaccgagat ccacttaacg ttactgaaat catcaaacag
61 cttgacgaat ctggatataa gatcgttggt gtcgatgtca gctccggagt tgagacaaat
121 ggtgttcagg atctcgataa gatacgttca tttgtccaag cagcaaagag tgccttctag
181 tgatttaata gctccatgtc aacaagaata aaacgcgttt cgggtttacc tcttccagat
241 acagctcatc tgcaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
301 caattataca tttaatacgc gatagaaaac aaaatatagc gcgtgtacgc atgtaacatt
361 atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcaagctt
421 tgaagaaaaa tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg
481 gaagacattt gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg
541 tgggaaattg gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga
601 taacagcaat acctgatcga ccaattccga tcatattcaa taacccttaa tataacttcg
661 tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc agccaagcta
721 gcttggctgc aggtcgagcg gccgcgatcc ggaaccctta atataacttc gtataatgta
781 tgctatacga agttattagg tccctcgacc ggccgcggcg gttgtttgca agaccgagaa
841 aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag
901 tttgattcca ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta
961 caatcaatca aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt
1021 ttatgtttag atccactagt ttctcggtac tatgcattac atgttaatta ttacatgctt
1081 aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct
1141 taagaaactt tattgccaaa tgtttgaacg atctgcaggt cgacggatca gatctcggtg
1201 acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca gaaacccacg
1261 tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg ggcatatccg
1321 agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc gaccacgctc
1381 ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga gcccagtccc
1441 gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc gtaggcgttg
1501 cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac ctcggcgacg
1561 agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg cggttcctgc
1621 ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat ggtgcagacc
1681 gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc tgggctcatg
1741 gttacttcct aatcgaagct ttgcttgggt agaataggta agtcagattg aatctgaaat
1801 aaagggagga agggcgaact taagaaggta tgaccgggtc gttcacttac cttgcttgac
1861 aaacgcacca agttatcgtg caccaagcag cagatgataa taatgtcctc gttcctgtct
1921 gctaataaga gtcacacttc gagcgccgcc gctactgcta caagtggggc tgatctgacc
1981 agttgcctaa atgaaccatc ttgtcaaacg acacaaattt tgtgctcacc gcctggacga
2041 ctaaaccaaa ataggcattc attgttgacc tccactagct ccagccaagc ccaaaaagtg
2101 ctccttcaat atcatcttct gtcgagtcta gagcacattc tgcggcctct gtgctctgca
2161 agccgcaaac tttcaccaat ggaccagaac tacctgtgaa attaataaca gacatactcc
2221 aagctgcctt tgtgtgctta atcacgtata ctcacgtgct caatagtcac caatgccctc
2281 cctcttggcc ctctcctttt cttttttcga ccgaattaat tcttgaagac gaaagggcct
2341 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
2401 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
2461 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
2521 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
2581 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
2641 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
2701 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
2761 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
2821 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
2881 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
2941 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
3001 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
3061 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
3121 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
3181 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
3241 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
3301 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
3361 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
3421 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
3481 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
3541 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
3601 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
3661 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
3721 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
3781 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
3841 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
3901 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
3961 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
4021 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
4081 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
4141 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
4201 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
4261 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
4321 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
4381 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
4441 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
4501 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
4561 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagagatct
4621 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
4681 atgcaaagca tgcggagaga cggacggacg cagagagaag ggctgagtaa taagcgccac
4741 tgcgccagac agctctggcg gctctgaggt gcagtggatg attattaatc cgggaccggc
4801 cgcccctccg ccccgaagtg gaaaggctgg tgtgcccctc gttgaccaag aatctattgc
4861 atcatcggag aatatggagc ttcatcgaat caccggcagt aagcgaagga gaatgtgaag
4921 ccaggggtgt atagccgtcg gcgaaatagc atgccattaa cctaggtaca gaagtccaat
4981 tgcttccgat ctggtaaaag attcacgaga tagtaccttc tccgaagtag gtagagcgag
5041 tacccggcgc gtaagctccc taattggccc atccggcatc tgtagggcgt ccaaatatcg
5101 tgcctctcct gctttgcccg gtgtatgaaa ccggaaaggc cgctcaggag ctggccagcg
5161 gcgcagaccg ggaacacaag ctggcagtcg acccatccgg tgctctgcac tcgacctgct
5221 gaggtccctc agtccctggt aggcagcttt gccccgtctg tccgcccggt gtgtcggcgg
5281 ggttgacaag gtcgttgcgt cagtccaaca tttgttgcca tattttcctg ctctccccac
5341 cagctgctct tttcttttct ctttcttttc ccatcttcag tatattcatc ttcccatcca
5401 agaaccttta atcaagctta tcgataccgt cgacctcgac tctagaggat cccccgggct
5461 gcaggaattc gatatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
5521 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
5581 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
5641 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
5701 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
5761 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
5821 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
5881 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
5941 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
6001 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
6061 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
6121 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
6181 gtaaatcaag cttatcgata ccgtcgacct cgaggggggg cccggtacc
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