北京华越洋生物
13581845453(微信)
7*24h全国热线:
15011481284
首页    载体及质粒    pENTR5'-UbCp
pENTR5

pENTR5'-UbCp

pENTR5@-UbCp

pENTR5'-UbCp

 



编号

载体名称

北京华越洋VECT90287

pENTR5'-UbCp

 

pENTR5'-UbCp载体基本信息:

启动子:

T7

复制子:

pUC ori

质粒分类:

哺乳细胞,GateWay入门载体

质粒大小:

3861bp

原核抗性:

Kan

克隆菌株:

DB3.1

培养条件:

37℃,有氧 LB

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:

M13F:TGTAAAACGACGGCCAGT

3'测序引物:

根据序列设计引物

 

pENTR5'-UbCp载体质粒图谱和多克隆位点信息:

 

 

pENTR5'-UbCp载体简介:

PENTR5-UbCp vector containing CCDb lethal gene, can make the common DH5alpha and other bacterial death, must use the DB3.1 feeling cell for plasmid amplification;CcdB sensitive OmniMAX2 cells are needed to construct the Gateway system when it is started..

 

pENTR5'-UbCp载体序列:

ORIGIN

        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA

       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAACGGAAGA

      121 GCGCCCAATA CGCAAACCGC CCTCTCCCCG CGCGTTGGCC GATTCATTAA ATGCAGCTGG

      181 CACGACAGGT TTTCCCGACT GGAAAGCGGG CAGTGGAGCG CAACGCAATT AATACCCGTA

      241 CCCGCTTACC CAGGAAAAGT TTGTAAAAAC GCAAAAAAGG CCATCCCTCC AGGATGGCCT

      301 TCTGCTTAAT TTGGATGCCT GGCAAGTTTA TGGGGGGGGT CTGCCCGCCA CCCTCCGGGC

      361 CGTTGCTTCC ACAACGTTCA AATCCGCTCC CCGCGGATTT GTCCTACTCA GGAGAGCGTT

      421 CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTTCCCG ACTGAGCCTT TCGTTTTATT

      481 TGATGCCTGG CAGTTCCCTA CTCTCGCGTT AACGCTAGCA TGGATGTTTT CCCAGTCACG

      541 ACGTTGTAAA ACGACGGCCA GTCTTAAGCT CGGGCCCGCG TTAACGCTAC CATGGAGCTC

      601 CAAATAATGA TTTTATTTTG ACTGATAGTG ACCTGTTCGT TGCAACAAAT TGATAAGCAA

      661 TGCTTTTTTA TAATGCCAAC TTTGTATAGA AAAGTTGGGC CTCGCGCCGG GTTTTGGCGC

      721 CTCCCGCGGG CGCCCCCCTC CTCACGGCGA GCGCTGCCAC GTCAGACGAA GGGCGCAGGA

      781 GCGTCCTGAT CCTTCCGCCC GGACGCTCAG GACAGCGGCC CGCTGCTCAT AAGACTCGGC

      841 CTTAGAACCC CAGTATCAGC AGAAGGACAT TTTAGGACGG GACTTGGGTG ACTCTAGGGC

      901 ACTGGTTTTC TTTCCAGAGA GCGGAACAGG CGAGGAAAAG TAGTCCCTTC TCGGCGATTC

      961 TGCGGAGGGA TCTCCGTGGG GCGGTGAACG CCGATGATTA TATAAGGACG CGCCGGGTGT

     1021 GGCACAGCTA GTTCCGTCGC AGCCGGGATT TGGGTCGCGG TTCTTGTTTG TGGATCGCTG

     1081 TGATCGTCAC TTGGTGAGTA GCGGGCTGCT GGGCTGGCCG GGGCTTTCGT GGCCGCCGGG

     1141 CCGCTCGGTG GGACGGAAGC GTGTGGAGAG ACCGCCAAGG GCTGTAGTCT GGGTCCGCGA

     1201 GCAAGGTTGC CCTGAACTGG GGGTTGGGGG GAGCGCAGCA AAATGGCGGC TGTTCCCGAG

     1261 TCTTGAATGG AAGACGCTTG TGAGGCGGGC TGTGAGGTCG TTGAAACAAG GTGGGGGGCA

     1321 TGGTGGGCGG CAAGAACCCA AGGTCTTGAG GCCTTCGCTA ATGCGGGAAA GCTCTTATTC

     1381 GGGTGAGATG GGCTGGGGCA CCATCTGGGG ACCCTGACGT GAAGTTTGTC ACTGACTGGA

     1441 GAACTCGGTT TGTCGTCTGT TGCGGGGGCG GCAGTTATGC GGTGCCGTTG GGCAGTGCAC

     1501 CCGTACCTTT GGGAGCGCGC GCCCTCGTCG TGTCGTGACG TCACCCGTTC TGTTGGCTTA

     1561 TAATGCAGGG TGGGGCCACC TGCCGGTAGG TGTGCGGTAG GCTTTTCTCC GTCGCAGGAC

     1621 GCAGGGTTCG GGCCTAGGGT AGGCTCTCCT GAATCGACAG GCGCCGGACC TCTGGTGAGG

     1681 GGAGGGATAA GTGAGGCGTC AGTTTCTTTG GTCGGTTTTA TGTACCTATC TTCTTAAGTA

     1741 GCTGAAGCTC CGGTTTTGAA CTATGCGCTC GGGGTTGGCG AGTGTGTTTT GTGAAGTTTT

     1801 TTAGGCACCT TTTGAAATGT AATCATTTGG GTCAATATGT AATTTTCAGT GTTAGACTAG

     1861 TAAATTGTCC GCTAAATTCT GGCCGTTTTT GGCTTTTTTG TTAGACCAAG TTTGTACAAA

     1921 AAAGTTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT

     1981 AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATGC AGTCACTATG AATCAACTAC

     2041 TTAGATGGTA TTAGTGACCT GTAGAATTCG AGCTCTAGAG CTGCAGGGCG GCCGCGATAT

     2101 CCCCTATAGT GAGTCGTATT ACATGGTCAT AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC

     2161 TCAAAATCTC TGATGTTACA TTGCACAAGA TAAAAATATA TCATCATGAA CAATAAAACT

     2221 GTCTGCTTAC ATAAACAGTA ATACAAGGGG TGTTATGAGC CATATTCAAC GGGAAACGTC

     2281 GAGGCCGCGA TTAAATTCCA ACATGGATGC TGATTTATAT GGGTATAAAT GGGCTCGCGA

     2341 TAATGTCGGG CAATCAGGTG CGACAATCTA TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA

     2401 GTTGTTTCTG AAACATGGCA AAGGTAGCGT TGCCAATGAT GTTACAGATG AGATGGTCAG

     2461 ACTAAACTGG CTGACGGAAT TTATGCCTCT TCCGACCATC AAGCATTTTA TCCGTACTCC

     2521 TGATGATGCA TGGTTACTCA CCACTGCGAT CCCCGGAAAA ACAGCATTCC AGGTATTAGA

     2581 AGAATATCCT GATTCAGGTG AAAATATTGT TGATGCGCTG GCAGTGTTCC TGCGCCGGTT

     2641 GCATTCGATT CCTGTTTGTA ATTGTCCTTT TAACAGCGAT CGCGTATTTC GTCTCGCTCA

     2701 GGCGCAATCA CGAATGAATA ACGGTTTGGT TGATGCGAGT GATTTTGATG ACGAGCGTAA

     2761 TGGCTGGCCT GTTGAACAAG TCTGGAAAGA AATGCATAAA CTTTTGCCAT TCTCACCGGA

     2821 TTCAGTCGTC ACTCATGGTG ATTTCTCACT TGATAACCTT ATTTTTGACG AGGGGAAATT

     2881 AATAGGTTGT ATTGATGTTG GACGAGTCGG AATCGCAGAC CGATACCAGG ATCTTGCCAT

     2941 CCTATGGAAC TGCCTCGGTG AGTTTTCTCC TTCATTACAG AAACGGCTTT TTCAAAAATA

     3001 TGGTATTGAT AATCCTGATA TGAATAAATT GCAGTTTCAT TTGATGCTCG ATGAGTTTTT

     3061 CTAATCAGAA TTGGTTAATT GGTTGTAACA CTGGCAGAGC ATTACGCTGA CTTGACGGGA

     3121 CGGCGCAAGC TCATGACCAA AATCCCTTAA CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC

     3181 AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTACTCTG

     3241 CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT

     3301 ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT

     3361 TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT

     3421 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG

     3481 GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC

     3541 GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA

     3601 GCATTGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG

     3661 CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA

     3721 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG

     3781 GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG

     3841 CTGGCCTTTT GCTCACATGT T

//

 

pENTR5'-UbCp其他相关信号通路报告载体:

pEF1/V5-HisA

pEGFP-N3

pEF-GFP

pEGFP-C3

pFLAG-CMV2

pEGFP-N2

pCI-NEO

pEGFP-N1

pCEP4

pEGFP-1

pVAX1

pECFP-C1

pcDNA3.1/V5-His B

pECFP-N1

pCDNA6-Myc-HisB

pDsRed2-Nuc

pCDNA3.1-3×HA

pDsRed2-ER

pCDNA3.1-3×HA

Top-Flash

pCDNA3.1(-)/Myc-His B

pSTAT3-TA-luc

pCDNA3.1(-)/Myc-His A

pSilencer2.1-U6 neo

pCDNA3.1(+)/Myc-His B

psiCHECK-2

pCDNA3.1(+)-Zeo

pRL-TK

pCDNA3.1(+)-EGFP

pRL-SV40

pCDNA3.1(+)

pRL-CMV

pCDNA3.1(-)

pp53-TA-luc

pBudCE4.1-hrGFP

pNF-KB-TA-LUC

pCAGGS-HA

pNF-kB-Luc

pVAX1-FLAG

pMIR-Reporter

pSV2-DHFR

pmirGLO

pCEP4/CAT

pGL4.79[hRluc/Neo]

pCAGGS

pGL4.75[hRluc/CMV]

pCAG-GFP

pGL4.73[hRluc /SV40]

pCDNA3.0

pGL4.29[luc2P/CRE/Hygro]

pCDNA3.0-HA

pGL4.13-luci2

pCDNA3.1(+)-3×FLAG

pGL4.13-luci2

pSV-Beta-Gal

pGL4.10[luc2]

SuperCos1

pGL3-Enhancer

pT2/VENUS

pGL3-Control

pAdVAntage

pGL3-Basic

pEYFP-Tubulin

pGL3-3.6kb human IFN-gamma luc

pEGFP-C1

pENTR5'-UbCp

pmGeos-M-N1

pBiFC-VN173

pEYFP-N1

pBiFC-VC155

pEYFP-C1

pBIFC-bFosVC155

pEGFP-C2

pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP)   VC155

pEYFP-Actin

Laconic/pcDNA3.1(-)

pDsRed2-Peroxi

Fop-Flash

pEBFP-C1

pTet-On-Advanced

pmGeos-M-C1

pmCherry-EGFP-LC3b-r

pCerulean-N1

pmCherry-EGFP-LC3b-m

pmKO2-N1

pmCherry-EGFP-LC3B-h

pMyosin-PSmOrange

pEGFP-LC3B-r

pTagBFP-N

pEGFP-LC3B-m

pCAG-DsRed

pEGFP-LC3B-h

pmCherry-C1

pEGFP-LC3B-c

pmCherry-N1

Super PiggyBac Transposase (PB200PA-1)

pEYFP-Golgi

pTol2-Promoter-T2A-EGFP

pEYFP-Mem

pSP64-Tol2-Transposase

pDsRed2-C1

PiggyBac Dual promoter (PB513B-1)

pEGFP-Actin

pBIND-ID

pEBFP-N1

pG5Luc

pDsRed2-N1

pFUSE-hIgG1e1-Fc2

pN3

pEE12.4

pEGFP-EcoRV

pEE6.4