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首页    载体及质粒    pKW08-Lx-Int
pKW08-Lx-Int

pKW08-Lx-Int

pKW08-Lx-Int

pKW08-Lx-Int

 



编号

载体名称

北京华越洋VECT75502

pKW08-Lx-Int

 

pKW08-Lx-Int载体基本信息:

复制子:

ColE1 ori

终止子:

rrnB T2 terminator,rrnB T1 terminator

质粒分类:

分枝杆菌质粒

质粒大小:

8535bp

质粒标签:

LuxA,LuxB

原核抗性:

潮霉素Hygromycin

克隆菌株:

DH5α

培养条件:

37℃,有氧,LB

表达宿主:

分枝杆菌

5'测序引物:

根据全序列设计

备注:

低拷贝

 

pKW08-Lx-Int载体图谱:

 

pKW08-Lx-Int载体序列:

ORIGIN

        1 actagttcta gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg

       61 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag

      121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag

      181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc

      241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc

      301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc

      361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt

      421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg

      481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat

      541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag

      601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt

      661 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc

      721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta

      781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag

      841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga

      901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa

      961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa

     1021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc

     1081 ccggacgtgg ccgaccagcc cgtcatcgtc aacgcctgac cgcggtgcgg acaggccgtg

     1141 tcgcgaccgg ccgtgcggaa ttaagccggc ccgtaccctg tgaatagagg tccgctgtga

     1201 cacaagaatc cctgttactt ctcgaccgta ttgattcgga tgattcctac gcgagcctgc

     1261 ggaacgacca ggaattctgg gagccgctgg cccgccgagc cctggaggag ctcgggctgc

     1321 cggtgccgcc ggtgctgcgg gtgcccggcg agagcaccaa ccccgtactg gtcggcgagc

     1381 ccgacccggt gatcaagctg ttcggcgagc actggtgcgg tccggagagc ctcgcgtcgg

     1441 agtcggaggc gtacgcggtc ctggcggacg ccccggtgcc ggtgccccgc ctcctcggcc

     1501 gcggcgagct gcggcccggc accggagcct ggccgtggcc ctacctggtg atgagccgga

     1561 tgaccggcac cacctggcgg tccgcgatgg acggcacgac cgaccggaac gcgctgctcg

     1621 ccctggcccg cgaactcggc cgggtgctcg gccggctgca cagggtgccg ctgaccggga

     1681 acaccgtgct caccccccat tccgaggtct tcccggaact gctgcgggaa cgccgcgcgg

     1741 cgaccgtcga ggaccaccgc gggtggggct acctctcgcc ccggctgctg gaccgcctgg

     1801 aggactggct gccggacgtg gacacgctgc tggccggccg cgaaccccgg ttcgtccacg

     1861 gcgacctgca cgggaccaac atcttcgtgg acctggccgc gaccgaggtc accgggatcg

     1921 tcgacttcac cgacgtctat gcgggagact cccgctacag cctggtgcaa ctgcatctca

     1981 acgccttccg gggcgaccgc gagatcctgg ccgcgctgct cgacggggcg cagtggaagc

     2041 ggaccgagga cttcgcccgc gaactgctcg ccttcacctt cctgcacgac ttcgaggtgt

     2101 tcgaggagac cccgctggat ctctccggct tcaccgatcc ggaggaactg gcgcagttcc

     2161 tctgggggcc gccggacacc gcccccggcg cctgacgccc cgggccgccc ggcgccgccc

     2221 ccggcccccg gcggccgccc ggagccccgc ccgcgctcgg gagccccggg cccgcgccga

     2281 agcccgctgc tgcgagcccg gagcgggccg gccgacggcg gtgcgggccc ggcggcggac

     2341 gctcagcagc ggcgggcgtg aaaggccctg gcatcctcga tcatctcctc cagggtggtc

     2401 ggccgagctt ccatcccagc tcggcaagga tcaacgagga cagtcgcacg acgaagttct

     2461 tctggatcgc gcccgtgctg gaagcactca acctcgaagc gtgtggttgc ggagccatct

     2521 agcaaccaca cgaaacatgc gcaacgaacc gcgcaacgaa caacgcctag aactggccct

     2581 agatgagctg actcgtatcg ttggtaaacc tagtttgacc agcatgtttt aactacgttc

     2641 ggtgagctgt caacggggcc tgtaacggca caacgaaccg tgcaacgaga gtggccacgg

     2701 atgccaccac aggcactaca acggagttcg ccacgtacat caccacaacc accgattctg

     2761 gcggtgagct ccccgatatt cagcggaaat ggcttggtat cgaccaagat tcgtagaacc

     2821 ccgtctcgtc tggctggtat tcaaaacggg cgcaacgaaa cacgcaacga gacaggcatg

     2881 gcccaaacca gaaaactagc gtctaccagg acttttacct gtccgacccg ttgcaacgga

     2941 accccccacg gaacccccgc gacacccgct ccccaattgt tgctagctgg cgggaacggc

     3001 aatgctcctg gcggcggcgg ggctggtgcc caggtctccg cacagagcgg cggtgctggc

     3061 gctcgcggcc aggcgtggtt cttcgcgtac tgacaagaaa cccccctctt taggactcag

     3121 tgtccttggg aggggggctt tttgcgtttc aggaggtctt ggccagcttg gacatcgcct

     3181 cagcgatagc ctcgtcgcgg gcctcagacg ccatctggta cttcatcgcc atcctaggag

     3241 tcgtgtgacc gagacgggcc atcagctcct tggtcgtcgc acctgcctga gcggcgaacg

     3301 tagcgccgac agcgcggagg tcgtggatgc ggagttccgg ccgaccgatc ttggcgtagc

     3361 cacgcttcag cgacttggtg aacgcggact tcgacagccg gttgccctgc gtcgtggtca

     3421 ccaggaatgc ctcggggccc ttgttcatct tcgtacggtc cttcatgtgc gctcggatca

     3481 tctccgcgac gtgaggcgga accgtcacag gacgcttcga ccggacggtc ttggcgttgc

     3541 caacgacgat cttgttcccc acgcgggaag cgccacggcg cacccggagc ttcatcgtca

     3601 tgccgtcgtc cacgatgtcc ttgcggcgaa gctcgatcag ctctccgaac cggaggctcg

     3661 tccacgccag gatgtatgcc gcgatccggt agtgctcgaa gatctcagcg gcgacgatgt

     3721 ccagctcctc aggcgtcagc gcctctacgt cgcgctcatc ggctgccttc tgctcgatcc

     3781 ggcacgggtt ctctgcgatc agcttgtcct cgaccgctgt gttcatcacc gcccggagga

     3841 cgttgtaggc atgccggcgg gcagtcgggt gcttcctacc catcccggcc caccacgcac

     3901 gcaccagagc tggcgtcatc tctgtgaccg ccacttcacc tagcaccggg tagatgcggc

     3961 gctccgcgtg cccgctgtac agatccctgg tgccgtctgc gaggtcgcgc tccacgagcc

     4021 acttccgggt gtactcctcc agcgtgatgg cgctggcggc tgccttcttc gcccggtcct

     4081 gtggaggggt ccaggtctcc atctcgatga gccgcttctc gcccgcgagc caggcttcgg

     4141 cgtccatctt gttgtcgtag gtctgcagcg cgtagtacct cacaccgtcc tgcgggttga

     4201 cgtatgaggc ttggatcctc ccgctgcgct gagtcttcag cgatccccat ccgcgacgtg

     4261 ccaactaggt ctcctctcgt cgtgaacaag gctaccgggt tgcaactcct gtgcaactct

     4321 caggcttcaa cgcgcttcta cgacctgcaa tttctttcca cttagaggat gcagccgaga

     4381 gggggtaaaa acctatcttg accggcccat atatgtggtc ggcagacacc cattcttcca

     4441 aactagctac gcgggttcga ttcccgtcgc ccgctccgct ggtcagaggg tgttttcgcc

     4501 ctctggccat ttttctttcc aggggtctgc aactcttgtg cgactcttct gacctgggca

     4561 tacgcggttg caacgcatcc ctgatctggc tactttcgat gctgacaaac gaatagagcc

     4621 ccccgcctgc gcgaacagac gaggggcatt cacaccagat tggagctggt gcagtgaaga

     4681 gaatagaccg ggacaaggtt gcaccgggag ttgcagcggt cggaaccctc gccgtcggcg

     4741 ggctggcgtt cgccctgtcg ttcacggctc tagctgatca ccgcggccat gatggcataa

     4801 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagtcgatc

     4861 gtacaccggt gtatggtccg acacgagctc caagtcagat atttcgctga ggggccaccc

     4921 cacaactgca cactcccccg ctctcccgtc gagccctggt ggtggaacac cagcgacagc

     4981 cgagcacccc caaccacctg taccaaccag gaggaacaca tgcgtcgttt cgaggacgtt

     5041 tccgggccgc tgagagccgc tgtggcggcc gtacacgccg ccttagaccc gttagacccc

     5101 ctgccgcctg aatgcgcggg tacgagccac acagcgcccg aacttacgga gctggtgggc

     5161 tcacctggct ttatggcgta cgaatcggct gtgtgcgacc tgttgggcga ggtgaggtac

     5221 gcgctactca cgctggcaag ggcgacacag ccgccccacc gagcccgcac ggccgcgcgc

     5281 ggtgtcaaca accgggtgag tcgtgcacac cagcaggtgt tcgaggcttg gctcgaagtg

     5341 caggacatcg tggcgaacgc cgcccgatga gccgcgcctt acgctggctg ccagccgttc

     5401 gcgggctggt tggtgcagcg cgtcgagcgg ttagaggccc tgcggtgttc caccaccgca

     5461 ggcctcgccc tttttaaggc tgaatttgct tgtctccgaa tccaactggc ttgtccaagg

     5521 gtgtatctac gcttaatcca aagttcaaac gaggggatta cacatgacca acttcgataa

     5581 cgttctcggc tcgatgatcc ccggcgggcc ccccctcgag gtcgacggta tcgataaaat

     5641 aaaaaagggg acctctaggg tccccaatta attagctaaa gcttttacga gtggtatttg

     5701 acgatgttgg cgtttaccac atcgataacc gctctttgct gcgctttatc ttccatcgac

     5761 tcaaaagaca tcaataggtc cgcggcacca caacactcaa tcgcaactcg cgctgcctga

     5821 gtactttctt cataagtacc gatggcattt tctgacaaca gctctcccat tttttgctca

     5881 aagtcggtat ttgagtaaga ttcacggaca aactcttcca aatacacgcg agcttctgcc

     5941 cttgctgctt caccatctac attttggttg accagcagcg tcagcttgtg tcgaacctga

     6001 ctaacatcga caccatgtgc ctgagcaact tcgtggtaca aaccggcgta ttcttttctt

     6061 tgagcgtttg agtcgtccca tctaaacacg agtggaagcc ctaacttagc cgcccattca

     6121 accacttctt tgctcgtcgc attcacaaat tgcgcaggac cgccttcagt gaacgcgtgt

     6181 gggttaacgg agattttagg aaaactataa aaatcattgt ttggatggca gtacccagta

     6241 gtgaatgaat cattgatgat cttgtgacac tcactgaaca actgaaactg agaatccgtt

     6301 ggtcgattaa agaagcgcat atctgcactc ttttcacaat cactaaagcc aaaggcaaaa

     6361 cggccttcac tcatttggtc aagtagacac gcttcttccg ccacacgtac tggatgatgc

     6421 gtggtaatga cgtgattcaa cgaagccact ttggcgttct ttgtcatacc aagtaaaaaa

     6481 ccagccactg ttagtggggc accaaccaca ccattgttcg agaaatggtt ttcgtaaaca

     6541 gccaacgtgt caaacttcaa ctgatctacg taatgtgcgg tatctaacat ttcttcgatg

     6601 acttgatcag aagaacgctt tgagttcata aaattgagga agaataatcc aaatttcatg

     6661 tctctttcct tttagaaaat attaattact gtttttcttt gagatatggc atcacatcag

     6721 actggaatag cttcatagat gcgataattt cttcttcaga accgtttgct tcaaaaccac

     6781 aacaaatatt gtcaataccc gtcgcatcaa tatcttgctg gataatcgcg atacactctt

     6841 caggcgtccc tactgggttg atttcgtagc tgtaatcaat tcggcgattg gtgtctttgt

     6901 ggcctttcaa cacaaaatca cgccattgac ctttattgaa gtcgtaacct tttgtttggt

     6961 cagagtcgtc aaaaatcttg gtggcattca cgtatgagtc gtaccaatgg cccaagaagt

     7021 tgcggcaaat atctttggct ctatttgagt catgatcgac ggaggtgatg taagacaaac

     7081 agtggtcaat cttagtcaca tcgtagccat gttcagtcgc gacttcgttg taaagatcaa

     7141 gctgcgcttt cttctcgtga gtgttgatga tccagcttag aatcattggt aggccacgct

     7201 ctgcagccca ttctgtcgtt gatgctgact ccgcgacgac ataaacagga gcaccacctt

     7261 gtgtgtaagc cgatggattc agttggattt tcgggaactt aatatgttcg ttatccgccg

     7321 cgatatagcc ttcattgaag ccttctttca tcaagtcata ccaacagtcc attaaggctc

     7381 ggctgttatc catgtctgta ccaaagacac gaaaatcttt atcgtacaaa ccgcgacaaa

     7441 taccaaaacg gaatcgtcct tttgacattt gatccagtag gtttacgtct tctgcttgtc

     7501 gaaccggatg ggcagtcggc aatacgatag ctgcagtgcc aacgttgagc gtttctgtcg

     7561 cacctaatag gtgtgcggca gcaacataag gattccctaa caacccaaat tcagtgaagt

     7621 ggtgctctag caaccaaacg gtgtcgaagc cacaaccttc agacgctttg cccagattaa

     7681 ccaatcgctt catcacttcg gtctgagata gctcaggtgg ctgataagtg aggaggaagt

     7741 ttccaaattt cataacattt cctccggatc ctgtcaggat tccacgatga gagtagcgag

     7801 cagtcagatg agagtagcga gcagtcactt tatctaagat aaagttatcg atgataaagt

     7861 gcaactatga ctcaccaccg gaagcgtctc gaccgcacgt ccgtgttgct cggggcccgg

     7921 caggtgctcg acgagactgg gctcgatggc ttcaccactc gcgcgctggc ggctcactta

     7981 caggtacagc agcccggcct ctactggcac ttccgcacga aggccgaact cctcgcagcg

     8041 ctggcgagcg acgtgcttga ccgagagcat cacgcttcct tccccgagtc cgacgagaaa

     8101 tgggacgcat ttctcttgcg aaacgcaaga agtttccgcc acgcactaca cgcggtccgt

     8161 gacggtgcgc gtctccacgc tgagcaccac cgcagaccat ccggcgacga caccgacgaa

     8221 ggagctgagg cttccggtca gcaagtcggg cttctcgtgt ctcagggatt cgatgaacag

     8281 acagccatca gcatgctcat cgcggtcagc cgatacaccg tcggtgtcgt actggaggag

     8341 caaaccgcag aggtcggcaa tgcgagcgtt cctgaacgcg accgggaatt cgatttcggc

     8401 ctgacagcac tgatcgacgg attctctcac tcccgcacag cgtcattgac ctcgagcgaa

     8461 tcgtgatcta gaggtttaaa cagtattaaa cgcaaaaagg ccatccgtca ggatggcctt

     8521 ctgcttagct aatca

//

 

 

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