pEGFP-LC3B-c

ꄴ上一个： pEGFP-LC3B-h

ꄲ下一个： Super PiggyBac Transposase (PB200PA-1)

pEGFP-LC3B-c

编号	载体名称
北京华越洋VECT90324	pEGFP-LC3B-c

pEGFP-LC3B-c载体基本信息：

启动子:	CMV
复制子:	pUC ori,f1 ori,SV40 ori
终止子:	SV40 poly(A) signal
质粒分类:	哺乳细胞，自噬表达载体
原核抗性:	Kan
筛选标记:	Neo
克隆菌株:	DH5α
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	无须诱导，瞬时表达
5'测序引物:	pEGFP-C-5:CATGGTCCTGCTGGAGTTCGTG
3'测序引物:	根据序列设计引物

pEGFP-LC3B-c载体质粒图谱和多克隆位点信息：

pEGFP-LC3B-c载体简介

pEGFP-LC3B-c载体序列:

ATGCCCTCGGAGAAGAGCTTCAAGCAGCGCCGCACCTTCGAGCAAAGAGTTGAAGATGTACGACT
GATCCGAGATCAGCATCCAACTAAAATACCGGTGATCATTGAAAGGTACAAGGGGGAGAAGCAG
CTTCCAGTTTTGGATAAGACCAAGTTCCTGGTGCCAGATCACGTCAACATGAGCGAGCTAATCAAAATC
ATCAGGCGACGCCTGCAGCTGAACTCCAACCAGGCCTTCTTCCTCCTGGTGAACGGACACAGCATGG
TGAGCGTCTCCACGCCCATCTCCGAGGTGTACGAGAGCGAGAAGGACGAGGACGGCTTCCTGTACA
TGGTGTATGCCTCTCAGGAGACTTTCGGAGTGCAATCTTCCGTCTAG
CAGGTCTCTCGGCAGGAGAGCTGTACAAGTACTCAGATCTCGAGCTCAA
GCTTATGCCCTCGGAGAAGAGCTTCAGGCAGCGCCGCACCTTCGAGC
AAAGAGTTGAAGATGTACGACTGATCCGAGATCAGCATCCAACTAAAATAC
CGGTGATCATTGAAAGGTACAAGGGGGAGAAGCAGCTTCCAGTTTTGGATA
AGACCAAGTTCCTGGTGCCAGATCACGTCAACATGAGCGAGCTAATCAAA
ATCATCAGGCGACGCCTGCAGCTGAACTCCAACCAGGCCTTCTTCCTCC
TGGTGAACGGACACAGCATGGTGAGCGTCTCCACGCCCATCTCCGAGGT
GTACGAGAGCGAGAAGGACGAGGACGGCTTCCTGTACATGGTGTATGCCT
CTCAGGAGACTTTCGGAGTGCAATCTTCCGTCTAGGTCGACGGTACCGCG
GGCCCGGGATCCACCGGATCTAGATAACTGATCATAATCAGCCATACCACA
TTTGTAGAGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGAAC
CTGAAACATAAAATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTA
TAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTT
TTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTAACG
CGTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAA
TCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATC
AAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAG
TCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTA
TCAGGGCGATGGCCCACTACGTGAACCTCACCCTAATCAAGTTTTTTGGGG
TCGAGGGGCCGAAAAGCCTAAATCGGAACCCTAAAGGGAGCCCCCGATTT
AAAGCTTGACGGGGAAAACCCGCGAACTGGCCAAAA

pEGFP-LC3B-c其他相关四环素调控载体：

pEF1/V5-HisA	pEGFP-N3
pEF-GFP	pEGFP-C3
pFLAG-CMV2	pEGFP-N2
pCI-NEO	pEGFP-N1
pCEP4	pEGFP-1
pVAX1	pECFP-C1
pcDNA3.1/V5-His B	pECFP-N1
pCDNA6-Myc-HisB	pDsRed2-Nuc
pCDNA3.1-3×HA	pDsRed2-ER
pCDNA3.1-3×HA	Top-Flash
pCDNA3.1(-)/Myc-His B	pSTAT3-TA-luc
pCDNA3.1(-)/Myc-His A	pSilencer2.1-U6 neo
pCDNA3.1(+)/Myc-His B	psiCHECK-2
pCDNA3.1(+)-Zeo	pRL-TK
pCDNA3.1(+)-EGFP	pRL-SV40
pCDNA3.1(+)	pRL-CMV
pCDNA3.1(-)	pp53-TA-luc
pBudCE4.1-hrGFP	pNF-KB-TA-LUC
pCAGGS-HA	pNF-kB-Luc
pVAX1-FLAG	pMIR-Reporter
pSV2-DHFR	pmirGLO
pCEP4/CAT	pGL4.79[hRluc/Neo]
pCAGGS	pGL4.75[hRluc/CMV]
pCAG-GFP	pGL4.73[hRluc /SV40]
pCDNA3.0	pGL4.29[luc2P/CRE/Hygro]
pCDNA3.0-HA	pGL4.13-luci2
pCDNA3.1(+)-3×FLAG	pGL4.13-luci2
pSV-Beta-Gal	pGL4.10[luc2]
SuperCos1	pGL3-Enhancer
pT2/VENUS	pGL3-Control
pAdVAntage	pGL3-Basic
pEYFP-Tubulin	pGL3-3.6kb human IFN-gamma luc
pEGFP-C1	pENTR5'-UbCp
pmGeos-M-N1	pBiFC-VN173
pEYFP-N1	pBiFC-VC155
pEYFP-C1	pBIFC-bFosVC155
pEGFP-C2	pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP) VC155
pEYFP-Actin	Laconic/pcDNA3.1(-)
pDsRed2-Peroxi	Fop-Flash
pEBFP-C1	pTet-On-Advanced
pmGeos-M-C1	pmCherry-EGFP-LC3b-r
pCerulean-N1	pmCherry-EGFP-LC3b-m
pmKO2-N1	pmCherry-EGFP-LC3B-h
pMyosin-PSmOrange	pEGFP-LC3B-r
pTagBFP-N	pEGFP-LC3B-m
pCAG-DsRed	pEGFP-LC3B-h
pmCherry-C1	pEGFP-LC3B-c
pmCherry-N1	Super PiggyBac Transposase (PB200PA-1)
pEYFP-Golgi	pTol2-Promoter-T2A-EGFP
pEYFP-Mem	pSP64-Tol2-Transposase
pDsRed2-C1	PiggyBac Dual promoter (PB513B-1)
pEGFP-Actin	pBIND-ID
pEBFP-N1	pG5Luc
pDsRed2-N1	pFUSE-hIgG1e1-Fc2
pN3	pEE12.4
pEGFP-EcoRV	pEE6.4