北京华越洋生物
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首页    载体及质粒    pSP64-Tol2-Transposase
pSP64-Tol2-Transposase

pSP64-Tol2-Transposase

pSP64-Tol2-Transposase

pSP64-Tol2-Transposase

 



编号

载体名称

北京华越洋VECT90330

pSP64-Tol2-Transposase

 

pSP64-Tol2-Transposase载体基本信息:

启动子:

SP6

质粒分类:

哺乳细胞,转座子载体

质粒大小:

5142bp

原核抗性:

Kan

筛选标记:

Neo

克隆菌株:

DH5α

培养条件:

37℃,有氧 LB

表达宿主:

哺乳细胞

诱导方式:

无须诱导,瞬时表达

5'测序引物:

sp6ATTTAGGTGACACTATAGAA

3'测序引物:

根据序列设计引物

 

pSP64-Tol2-Transposase载体质粒图谱和多克隆位点信息:

 

 

pSP64-Tol2-Transposase载体简介

pSP64-Tol2-Transposase载体序列:

ORIGIN

        1 GAATACAAGC TTGGGCTGCA GGTCGACATG TTCATTGGTC CTTTGGAAGT GACGTCATGT

       61 CACATCTATT ACCACAATGC ACAGCACCTT GACCTGGAAA TTAGGGAAAT TATAACAGTC

      121 AATCAGTGGA AGAAAATGGA GGAAGTATGT GATTCATCAG CAGCTGCGAG CAGCACAGTC

      181 CAAAATCAGC CACAGGATCA AGAGCACCCG TGGCCGTATC TTCGCGAATT CTTTTCTTTA

      241 AGTGGTGTAA ATAAAGATTC ATTCAAGATG AAATGTGTCC TCTGTCTCCC GCTTAATAAA

      301 GAAATATCGG CCTTCAAAAG TTCGCCATCA AACCTAAGGA AGCATATTGA GAGAATGCAC

      361 CCAAATTACC TCAAAAACTA CTCTAAATTG ACAGCACAGA AGAGAAAGAT CGGGACCTCC

      421 ACCCATGCTT CCAGCAGTAA GCAACTGAAA GTTGACTCAG TTTTCCCAGT CAAACATGTG

      481 TCTCCAGTCA CTGTGAACAA AGCTATATTA AGGTACATCA TTCAAGGACT TCATCCTTTC

      541 AGCACTGTTG ATCTGCCATC ATTTAAAGAG CTGATTAGTA CACTGCAGCC TGGCATTTCT

      601 GTCATTACAA GGCCTACTTT ACGCTCCAAG ATAGCTGAAG CTGCTCTGAT CATGAAACAG

      661 AAAGTGACTG CTGCCATGAG TGAAGTTGAA TGGATTGCAA CCACAACGGA TTGTTGGACT

      721 GCACGTAGAA AGTCATTCAT TGGTGTAACT GCTCACTGGA TCAACCCTGG AAGTCTTGAA

      781 AGACATTCCG CTGCACTTGC CTGCAAAAGA TTAATGGGCT CTCATACTTT TGAGGTACTG

      841 GCCAGTGCCA TGAATGATAT CCACTCAGAG TATGAAATAC GTGACAAGGT TGTTTGCACA

      901 ACCACAGACA GTGGTTCCAA CTTTATGAAG GCTTTCAGAG TTTTTGGTGT GGAAAACAAT

      961 GATATCGAGA CTGAGGCAAG AAGGTGTGAA AGTGATGACA CTGATTCTGA AGGCTGTGGT

     1021 GAGGGAAGTG ATGGTGTGGA ATTCCAAGAT GCCTCACGAG TCCTGGACCA AGACGATGGC

     1081 TTCGAATTCC AGCTACCAAA ACATCAAAAG TGTGCCTGTC ACTTACTTAA CCTAGTCTCA

     1141 AGCGTTGATG CCCAAAAAGC TCTCTCAAAT GAACACTACA AGAAACTCTA CAGATCTGTC

     1201 TTTGGCAAAT GCCAAGCTTT ATGGAATAAA AGCAGCCGAT CGGCTCTAGC AGCTGAAGCT

     1261 GTTGAATCAG AAAGCCGGCT TCAGCTTTTA AGGCCAAACC AAACGCGGTG GAATTCAACT

     1321 TTTATGGCTG TTGACAGAAT TCTTCAAATT TGCAAAGAAG CAGGAGAAGG CGCACTTCGG

     1381 AATATATGCA CCTCTCTTGA GGTTCCAATG TTTAATCCAG CAGAAATGCT GTTCTTGACA

     1441 GAGTGGGCCA ACACAATGCG TCCAGTTGCA AAAGTACTCG ACATCTTGCA AGCGGAAACG

     1501 AATACACAGC TGGGGTGGCT GCTGCCTAGT GTCCATCAGT TAAGCTTGAA ACTTCAGCGA

     1561 CTCCACCATT CTCTCAGGTA CTGTGACCCA CTTGTGGATG CCCTACAACA AGGAATCCAA

     1621 ACACGATTCA AGCATATGTT TGAAGATCCT GAGATCATAG CAGCTGCCAT CCTTCTCCCT

     1681 AAATTTCGGA CCTCTTGGAC AAATGATGAA ACCATCATAA AACGAGGCAT GGACTACATC

     1741 AGAGTGCATC TGGAGCCTTT GGACCACAAG AAGGAATTGG CCAACAGTTC ATCTGATGAT

     1801 GAAGATTTTT TCGCTTCTTT GAAACCGACA ACACATGAAG CCAGCAAAGA GTTGGATGGA

     1861 TATCTGGCCT GTGTTTCAGA CACCAGGGAG TCTCTGCTCA CGTTTCCTGC TATTTGCAGC

     1921 CTCTCTATCA AGACTAATAC ACCTCTTCCC GCATCGGCTG CCTGTGAGAG GCTTTTCAGC

     1981 ACTGCAGGAT TGCTTTTCAG CCCCAAAAGA GCTAGGCTTG ACACTAACAA TTTTGAGAAT

     2041 CAGCTTCTAC TGAAGTTAAA TCTGAGGTTT TACAACTTTG AGGGCGGCGG CAAGAAGAAG

     2101 CGCAAGGTGG GCTAGGGATC CCCGGGCGAG CTCCCAAAAA AAAAAAAAAA AAAAAAAAAA

     2161 AAAAACCGAA TTCTCTAGAG GTACCCCATG GCTCGAGGAA TTCGTAATCA TGTCATAGCT

     2221 GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT

     2281 AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC

     2341 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG

     2401 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT

     2461 GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT

     2521 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC

     2581 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA

     2641 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA

     2701 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC

     2761 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG

     2821 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC

     2881 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG

     2941 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT

     3001 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT

     3061 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG

     3121 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC

     3181 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA

     3241 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC

     3301 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC

     3361 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT

     3421 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT

     3481 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT

     3541 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC

     3601 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA

     3661 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG

     3721 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT

     3781 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC

     3841 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT

     3901 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG

     3961 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC

     4021 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC

     4081 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT

     4141 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG

     4201 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG

     4261 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA

     4321 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCT AAGAAACCAT

     4381 TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG AGGCCCTTTC GTCTCGCGCG

     4441 TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG

     4501 TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG

     4561 GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATTC

     4621 GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT

     4681 TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG

     4741 CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG

     4801 CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC

     4861 CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCTG GCTAGCGATG ACCCTGCTGA

     4921 TTGGTTCGCT GACCATTTCC GGGTGCGGGA CGGCGTTACC AGAAACTCAG AAGGTTCGTC

     4981 CAACCAAACC GACTCTGACG GCAGTTTACG AGAGAGATGA TAGGGTCTGC TTCAGTAAGC

     5041 CAGATGCTAC ACAATTAGGC TTGTACATAT TGTCGTTAGA ACGCGGCTAC AATTAATACA

     5101 TAACCTTATG TATCATACAC ATACGATTTA GGTGACACTA TA

//

 

pSP64-Tol2-Transposase其他相关转座子载体:

pEF1/V5-HisA

pEGFP-N3

pEF-GFP

pEGFP-C3

pFLAG-CMV2

pEGFP-N2

pCI-NEO

pEGFP-N1

pCEP4

pEGFP-1

pVAX1

pECFP-C1

pcDNA3.1/V5-His B

pECFP-N1

pCDNA6-Myc-HisB

pDsRed2-Nuc

pCDNA3.1-3×HA

pDsRed2-ER

pCDNA3.1-3×HA

Top-Flash

pCDNA3.1(-)/Myc-His B

pSTAT3-TA-luc

pCDNA3.1(-)/Myc-His A

pSilencer2.1-U6 neo

pCDNA3.1(+)/Myc-His B

psiCHECK-2

pCDNA3.1(+)-Zeo

pRL-TK

pCDNA3.1(+)-EGFP

pRL-SV40

pCDNA3.1(+)

pRL-CMV

pCDNA3.1(-)

pp53-TA-luc

pBudCE4.1-hrGFP

pNF-KB-TA-LUC

pCAGGS-HA

pNF-kB-Luc

pVAX1-FLAG

pMIR-Reporter

pSV2-DHFR

pmirGLO

pCEP4/CAT

pGL4.79[hRluc/Neo]

pCAGGS

pGL4.75[hRluc/CMV]

pCAG-GFP

pGL4.73[hRluc /SV40]

pCDNA3.0

pGL4.29[luc2P/CRE/Hygro]

pCDNA3.0-HA

pGL4.13-luci2

pCDNA3.1(+)-3×FLAG

pGL4.13-luci2

pSV-Beta-Gal

pGL4.10[luc2]

SuperCos1

pGL3-Enhancer

pT2/VENUS

pGL3-Control

pAdVAntage

pGL3-Basic

pEYFP-Tubulin

pGL3-3.6kb human IFN-gamma luc

pEGFP-C1

pENTR5'-UbCp

pmGeos-M-N1

pBiFC-VN173

pEYFP-N1

pBiFC-VC155

pEYFP-C1

pBIFC-bFosVC155

pEGFP-C2

pBiFC-bFOSDelataZipVC155/pBiFC-bFos(deltaZIP)   VC155

pEYFP-Actin

Laconic/pcDNA3.1(-)

pDsRed2-Peroxi

Fop-Flash

pEBFP-C1

pTet-On-Advanced

pmGeos-M-C1

pmCherry-EGFP-LC3b-r

pCerulean-N1

pmCherry-EGFP-LC3b-m

pmKO2-N1

pmCherry-EGFP-LC3B-h

pMyosin-PSmOrange

pEGFP-LC3B-r

pTagBFP-N

pEGFP-LC3B-m

pCAG-DsRed

pEGFP-LC3B-h

pmCherry-C1

pEGFP-LC3B-c

pmCherry-N1

Super PiggyBac Transposase (PB200PA-1)

pEYFP-Golgi

pTol2-Promoter-T2A-EGFP

pEYFP-Mem

pSP64-Tol2-Transposase

pDsRed2-C1

PiggyBac Dual promoter (PB513B-1)

pEGFP-Actin

pBIND-ID

pEBFP-N1

pG5Luc

pDsRed2-N1

pFUSE-hIgG1e1-Fc2

pN3

pEE12.4

pEGFP-EcoRV

pEE6.4