pBIND-ID
pBIND-ID
编号 | 载体名称 |
北京华越洋VECT90334 | pBIND-ID |
pBIND-ID载体基本信息:
启动子: | T7,CMV |
复制子: | pUC ori,f1 ori |
终止子: | SV40 poly(A) signal |
质粒分类: | 哺乳细胞,哺乳细胞双杂交载体 |
原核抗性: | Amp |
克隆菌株: | DH5α |
培养条件: | 37℃,有氧 LB |
表达宿主: | 哺乳细胞 |
诱导方式: | 无须诱导,瞬时表达 |
5'测序引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
3'测序引物: | 根据序列设计引物 |
pBIND-ID载体质粒图谱和多克隆位点信息:
pBIND-ID载体简介:
The protein product of the Id cDNA sequence in the pBIND-Id Vector is known to interact with the protein product of the MyoD cDNA sequence in the pACT-MyoD Vector.
pBIND-ID载体序列:
GGCTCGTTTAGTGACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCA
CAGTTAAATTGCTAACGCAGTCAGTGCTTCTGACACGACAGTCTCGAACTTAA
GCTGCAGTGACTCTCTTAAGGTAGCCTTGCAGAAGTTGGTCGTGAGGCACTGG
GCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAAAAACTGG
GCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTT
ACTGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTA
CAGCTCTTAAGGCTAGAGTACTTAATACGACTCACTATAGGCTAGCCAGCTTG
AAGCAAGCCTCCTGAAAGATGAAGCTACTGTCTTCTATCGAACAAGCATGCGA
TATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCA
AGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCT
CCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGA
ACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAA
TGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGAT
AATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATAT
GCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGA
GTAGTAACAAAGGTCAAAGACAGTTGACTGTATCGCCGGAATTCCCGGGGATC
CGTCGACATAAATTCCCACTTGGTCTGTCAGAGCAAAGCGTGGCCATCTCGCG
CTGCGCTGGGACGCGCCTGCCCGCCTTGCTGGACGAGCAGCAGTGAACGTCCT
GCTCTACGACATGAACGGCTGCTACTCACGCCTCAAGGAGCTGGTG
pBIND-ID其他相关哺乳细胞双杂交载体: