北京华越洋生物
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首页    载体及质粒    pQE-14
pQE-14

pQE-14

pQE-14

pQE-14

 



编号

载体名称

北京华越洋VECT90093

pQE-14

 

pQE-14  pQE系列表达载体基本信息:

启动子:

T5

复制子:

ColE1 ori

终止子:

Lambda t0 terminatorrrnB T1

质粒分类:

大肠杆菌载体;pQE系列表达质粒

质粒大小:

4002bp

原核抗性:

氨苄青霉素Amp

克隆菌株:

DH5α

培养条件:

37℃,有氧,LB

表达宿主:

M15

诱导方式:

IPTG或乳糖及其类似物

5'测序引物:

pQE30-F:   AGCGGATAACAATTTCACACAG

3'测序引物:

pQE30-R:   TTCTGAGGTCATTACTGGATC

 

pQE-14载体质粒图谱和多克隆位点信息:

 

 

pQE-14载体简介

pQE-14载体序列:

ORIGIN
        1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
       61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAAG AGGAGAAATT AACTATGAGA
      121 GGATCGCATC ACCATCACCA TCACGGATCC GGCATCATGG TTCGACCATT GAACTCGATC
      181 GTCGCCGTGT CCCAAAATAT GGGGATTGGC AAGAACGGAG ACCTACCCTG GCCTCCGCTC
      241 AGGAACGAGT TCAAGTACTT CCAAAGAATG ACCACAACCT CTTCAGTGGA AGGTAAACAG
      301 AATCTGGTGA TTATGGGTAG GAAAACCTGG TTCTCCATTC CTGAGAAGAA TCGACCTTTA
      361 AAGGACAGAA TTAATATAGT TCTCAGTAGA GAACTCAAAG AACCACCACG AGGAGCTCAT
      421 TTTCTTGCCA AAAGTTTGGA TGATGCCTTA AGACTTATTG AACAACCGGA ATTGGCAAGT
      481 AAAGTAGACA TGGTTTGGAT AGTCGGAGGC AGTTCTGTTT ACCAGGAAGC CATGAATCAA
      541 CCAGGCCACC TTAGACTCTT TGTGACAAGG ATCATGCAGG AATTTGAAAG TGACACGTTT
      601 TTCCCAGAAA TTGATTTGGG GAAATATAAA CTTCTCCCAG AATACCCAGG CGTCCTCTCT
      661 GAGGTCCAGG AGGAAAAAGG CATCAAGTAT AAGTTTGAAG TCTACGAGAA GAAAGGTTCA
      721 GATCTTAAGC TTAATTAGCT GAGCTTGGAC TCCTGTTGAT AGATCCAGTA ATGACCTCAG
      781 AACTCCATCT GGATTTGTTC AGAACGCTCG GTTGCCGCCG GGCGTTTTTT ATTGGTGAGA
      841 ATCCAAGCTA GCTTGGCGAG ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC
      901 ACTGGATATA CCACCGTTGA TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT
      961 CAGTCAGTTG CTCAATGTAC CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA
     1021 AAGACCGTAA AGAAAAATAA GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC
     1081 CTGATGAATG CTCATCCGGA ATTTCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG
     1141 GATAGTGTTC ACCCTTGTTA CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC
     1201 TGGAGTGAAT ACCACGACGA TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG
     1261 TGTTACGGTG AAAACCTGGC CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC
     1321 TCAGCCAATC CCTGGGTGAG TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC
     1381 TTCTTCGCCC CCGTTTTCAC CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG
     1441 CCGCTGGCGA TTCAGGTTCA TCATGCCGTC TGTGATGGCT TCCATGTCGG CAGAATGCTT
     1501 AATGAATTAC AACAGTACTG CGATGAGTGG CAGGGCGGGG CGTAATTTTT TTAAGGCAGT
     1561 TATTGGTGCC CTTAAACGCC TGGGGTAATG ACTCTCTAGC TTGAGGCATC AAATAAAACG
     1621 AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT
     1681 CCTGAGTAGG ACAAATCCGC CGCTCTAGAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA
     1741 AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG
     1801 GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT
     1861 GACCCAGTCA CGTAGCGATA GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG
     1921 ATTGTACTGA GAGTGCACCA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA
     1981 TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCTGTCGG
     2041 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
     2101 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG
     2161 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA
     2221 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT
     2281 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC
     2341 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG
     2401 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC
     2461 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA
     2521 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG
     2581 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT
     2641 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC
     2701 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA
     2761 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA
     2821 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT
     2881 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC
     2941 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGCT
     3001 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT
     3061 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG
     3121 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT
     3181 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT
     3241 GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC
     3301 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT
     3361 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG
     3421 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG
     3481 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT
     3541 TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC
     3601 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT
     3661 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT
     3721 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG
     3781 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT
     3841 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG
     3901 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA
     3961 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AC
//

 

pQE-14  其他相关pQE系列表达载体:

pETBlue-2

pQE-30   UA   (linearized)

pET30a-EcoRV

pQE-22

pET28a-SUMO

pQE-19

pET28a-OFP

pQE-18

pET28a-mCherry

pQE-17

pET28a-EYFP

pQE-15

pET28a-EGFP

pQE-14

pET28a-ECFP

pQE-13

pET28a-EBFP

pQE-12

pET28a-DsRed2

pQE-11

pTrc-CKS

pQE-10

pMBP-P

pQE-8

pMBP-C

pQE-7

pLpp-OmpA

pQE-6

pLLP-STII/Plpp-STII

pQE-5

pET-Trx

pQE-4

pETM-30

pQE-3

pETM-11

pCold-SUMO-His-FLAG

pET-GST

pCold-SUMO-His-FLAG

pET-DsbA

pCold-SUMO

pET22b-EGFP

pCold-NusA

TAGZyme     pQE-2

pCold-MBP

TAGZyme     pQE-1

pCold-DsbA

pQE-TriSystem-7

pUC57-Kan

pQE-TriSystem-6

pUC57-Simple

pQE-TriSystem-5

pUC57

pQE-TriSystem     Strep

pUC119

pQE-TriSystem     His-Strep2

pUC118

pQE-TriSystem     His-Strep1

pJET1.2   T   Recombined

pQE-T7-2

pMD-19TSimple

pQE-T7-1

pGEM-3Zf(+)

pQE-T7(cloning     region)

pBluescript     II SK(+)

pQE-100     DoubleTaq

pBluescript     II KS(+)

pQE-82L-Kan

pUC19

pQE-81L-Kan

pUC18

pQE-80L-Kan

pMD-18T

pQE-80

pUC57-Tac-mCherry

pQE-52

pTYB21

pQE-51

pTYB4

pQE-50

pTrcHisC

pQE-42

pTrcHisB

pQE-40

pTrcHisA

pQE-30   Xa

pTrcHis2C

pQE-30   UA

pTrcHis2B

pTrcHis2A

pBAD/Myc-HisA

pRSETC

pBAD/His     /LacZ

pRSETB

pBAD/His-C

pMCSG19

pBAD/His-B

pMCSG9

pBAD/His-A

pLAFR-3

pBAD30

pEGFP-Lac

pGEX-Zsgreen1

pDrive

pGEX-TagRFP

pBV222

pGEX-His

pBAD/Myc-HisC

pGEX-20T

pBAD/Myc-HisB

pGEX-1λT     EcoRI/BAP