北京华越洋生物
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首页    载体及质粒    pQE-10
pQE-10

pQE-10

pQE-10

pQE-10

 



编号

载体名称

北京华越洋VECT90098

pQE-10

 

pQE-10  pQE系列表达载体基本信息:

启动子:

T5

复制子:

ColE1 ori

终止子:

Lambda t0 terminatorrrnB T1

质粒分类:

大肠杆菌载体;pQE系列表达质粒

质粒大小:

3442bp

原核抗性:

氨苄青霉素Amp

克隆菌株:

DH5α

培养条件:

37℃,有氧,LB

表达宿主:

M15

诱导方式:

IPTG或乳糖及其类似物

5'测序引物:

pQE30-F:   AGCGGATAACAATTTCACACAG

3'测序引物:

pQE30-R:   TTCTGAGGTCATTACTGGATC

 

pQE-10载体质粒图谱和多克隆位点信息:

 

 

pQE-10载体简介

pQE-10载体序列:

ORIGIN
        1 CTCGAGAAAT CATAAAAAAT TTATTTGCTT TGTGAGCGGA TAACAATTAT AATAGATTCA
       61 ATTGTGAGCG GATAACAATT TCACACAGAA TTCATTAAAG AGGAGAAATT AACTATGAGA
      121 GGATCTCACC ATCACCATCA CCATACGGAT CCGTCGACCT GCAGCCAAGC TTAATTAGCT
      181 GAGCTTGGAC TCCTGTTGAT AGATCCAGTA ATGACCTCAG AACTCCATCT GGATTTGTTC
      241 AGAACGCTCG GTTGCCGCCG GGCGTTTTTT ATTGGTGAGA ATCCAAGCTA GCTTGGCGAG
      301 ATTTTCAGGA GCTAAGGAAG CTAAAATGGA GAAAAAAATC ACTGGATATA CCACCGTTGA
      361 TATATCCCAA TGGCATCGTA AAGAACATTT TGAGGCATTT CAGTCAGTTG CTCAATGTAC
      421 CTATAACCAG ACCGTTCAGC TGGATATTAC GGCCTTTTTA AAGACCGTAA AGAAAAATAA
      481 GCACAAGTTT TATCCGGCCT TTATTCACAT TCTTGCCCGC CTGATGAATG CTCATCCGGA
      541 ATTTCGTATG GCAATGAAAG ACGGTGAGCT GGTGATATGG GATAGTGTTC ACCCTTGTTA
      601 CACCGTTTTC CATGAGCAAA CTGAAACGTT TTCATCGCTC TGGAGTGAAT ACCACGACGA
      661 TTTCCGGCAG TTTCTACACA TATATTCGCA AGATGTGGCG TGTTACGGTG AAAACCTGGC
      721 CTATTTCCCT AAAGGGTTTA TTGAGAATAT GTTTTTCGTC TCAGCCAATC CCTGGGTGAG
      781 TTTCACCAGT TTTGATTTAA ACGTGGCCAA TATGGACAAC TTCTTCGCCC CCGTTTTCAC
      841 CATGGGCAAA TATTATACGC AAGGCGACAA GGTGCTGATG CCGCTGGCGA TTCAGGTTCA
      901 TCATGCCGTC TGTGATGGCT TCCATGTCGG CAGAATGCTT AATGAATTAC AACAGTACTG
      961 CGATGAGTGG CAGGGCGGGG CGTAATTTTT TTAAGGCAGT TATTGGTGCC CTTAAACGCC
     1021 TGGGGTAATG ACTCTCTAGC TTGAGGCATC AAATAAAACG AAAGGCTCAG TCGAAAGACT
     1081 GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG ACAAATCCGC
     1141 CGCTCTAGAG CTGCCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC
     1201 TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG
     1261 GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCGCAGCCAT GACCCAGTCA CGTAGCGATA
     1321 GCGGAGTGTA TACTGGCTTA ACTATGCGGC ATCAGAGCAG ATTGTACTGA GAGTGCACCA
     1381 TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA TACCGCATCA GGCGCTCTTC
     1441 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCTGTCGG CTGCGGCGAG CGGTATCAGC
     1501 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT
     1561 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT
     1621 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG
     1681 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC
     1741 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT
     1801 GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA
     1861 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA
     1921 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA
     1981 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA
     2041 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT
     2101 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
     2161 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
     2221 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
     2281 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC
     2341 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC
     2401 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGCT GCCTGACTCC CCGTCGTGTA
     2461 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA
     2521 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG
     2581 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC
     2641 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT
     2701 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG
     2761 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT
     2821 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA
     2881 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA
     2941 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA
     3001 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG
     3061 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC
     3121 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG
     3181 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT
     3241 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
     3301 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
     3361 GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT
     3421 CACGAGGCCC TTTCGTCTTC AC
//

pQE-10其他相关pQE系列表达载体:

pETBlue-2

pQE-30   UA   (linearized)

pET30a-EcoRV

pQE-22

pET28a-SUMO

pQE-19

pET28a-OFP

pQE-18

pET28a-mCherry

pQE-17

pET28a-EYFP

pQE-15

pET28a-EGFP

pQE-14

pET28a-ECFP

pQE-13

pET28a-EBFP

pQE-12

pET28a-DsRed2

pQE-11

pTrc-CKS

pQE-10

pMBP-P

pQE-8

pMBP-C

pQE-7

pLpp-OmpA

pQE-6

pLLP-STII/Plpp-STII

pQE-5

pET-Trx

pQE-4

pETM-30

pQE-3

pETM-11

pCold-SUMO-His-FLAG

pET-GST

pCold-SUMO-His-FLAG

pET-DsbA

pCold-SUMO

pET22b-EGFP

pCold-NusA

TAGZyme     pQE-2

pCold-MBP

TAGZyme     pQE-1

pCold-DsbA

pQE-TriSystem-7

pUC57-Kan

pQE-TriSystem-6

pUC57-Simple

pQE-TriSystem-5

pUC57

pQE-TriSystem     Strep

pUC119

pQE-TriSystem     His-Strep2

pUC118

pQE-TriSystem     His-Strep1

pJET1.2   T   Recombined

pQE-T7-2

pMD-19TSimple

pQE-T7-1

pGEM-3Zf(+)

pQE-T7(cloning     region)

pBluescript     II SK(+)

pQE-100     DoubleTaq

pBluescript     II KS(+)

pQE-82L-Kan

pUC19

pQE-81L-Kan

pUC18

pQE-80L-Kan

pMD-18T

pQE-80

pUC57-Tac-mCherry

pQE-52

pTYB21

pQE-51

pTYB4

pQE-50

pTrcHisC

pQE-42

pTrcHisB

pQE-40

pTrcHisA

pQE-30   Xa

pTrcHis2C

pQE-30   UA

pTrcHis2B

pTrcHis2A

pBAD/Myc-HisA

pRSETC

pBAD/His     /LacZ

pRSETB

pBAD/His-C

pMCSG19

pBAD/His-B

pMCSG9

pBAD/His-A

pLAFR-3

pBAD30

pEGFP-Lac

pGEX-Zsgreen1

pDrive

pGEX-TagRFP

pBV222

pGEX-His

pBAD/Myc-HisC

pGEX-20T

pBAD/Myc-HisB

pGEX-1λT     EcoRI/BAP